Rorug01G0234000

Methionine adenosyltransferase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001020
Physical Location & Seq
Forward (+)
33654181 .. 33655218
1038 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0234000.1

Sequence Viewer

Length: 1038 bp
ATGAACAGGGATCGATGCCAAAGTTATGGTTGGTTGAGGAACTGGTCCCTTCCTATTCCTCCTAAGCTCAAAATATTTCTCTGGTCTTTTGTCTCAAGCAAGCTGCTAACAAATGAACATCGTTTTAAACGAAAGCTCACATCCAATCCTTCCTGCCAGTTCTGCATAAACACCAAGAAAACTATGTTACATCTCTTTCGAGACTGTCCCAAGGCTAAGCAGGTTTGGCATTCCTTTGCTATCCCTGCCAACATGCTAAATACTTTCAATTTAAATTGGGAAGACTGGATTTTTGCCAACCTGTTGCAAAAAGGCTATCACATGAATAACTTGAACTGGAATATTTTCTTCATCTTCTGCTGTTGGTTTATTTGGAAATGGAGGTGCAAGAGTGTTTTTGATGTGGAGTTCCGTTTTCCCTATAATCCCTCTGAAGTTATCTTTAACTATGCTACTGATTGGGTTAAGGCTAATTCTAAGCCTAATACTCATTTGATTAGAAATGCTGATATGCTTCCTTGGCTTAGACCTGCTACTGGAAACCACAAACTTAATGTTGATGGATCCAGAGCCAGCAATGGTGCTATTGGAGCAGGTGGAGTTATAAGAGATGACACTGGGGCTTGGTGTGGTGGCTTTATGATTAATGTTGGGGCAGGTGAGGTCCTTCAAGCTGAGTCCTGGGGCCTCTATCATGGTCTGCAACTAGCGTTGAGCCTAAAGGTTACTAAATTGGAAGTTGAATCTGACTCCTCTGTGCTCATTAATCTTATGCATAGTGACAATATTAATCTCCATCCCTTGGGAACGTTGATCATGAACTGTAGACAACTGATGCATGAGTTTGAGTTCATCCAGATTAAGCACATCAAGCGTGAGCGCAACATGGTTGCTGATCTCCTAGCTAAGAACAGTATCTTGCATGCTAAAGGAATCTGGATCCTGCAGGACCCCCCTGCTTTGATCACTGAATGCCTTTTGGATGATATAATTGGGGTTCCCAGAGCTAGAGGCTTTAAGGCCAGGGGTGCTGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RmlD_sub_bind

Protein Analysis

345

Amino Acids

39.72

Weight (kDa)

9.36

Isoelectric Point (pI)

35.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 11 - 76 4.7e-14 zinc-binding in reverse transcriptase
RVT_3 PF13456 185 - 304 1.2e-26 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 605
AarI CACCTGC 2 cut(s) 584, 647
Acc36I ACCTGC 4 cut(s) 211, 538, 584, 647
AccB7I CCANNNNNTGG 1 cut(s) 802
AccI GTMKAC 1 cut(s) 826
AclI AACGTT 1 cut(s) 809
AclWI GGATC 5 cut(s) 18, 558, 571, 934, 947
AcuI CTGAAG 1 cut(s) 453
AfiI CCNNNNNNNGG 2 cut(s) 536, 802
AgsI TTSAA 4 cut(s) 268, 334, 671, 743
AjnI CCWGG 2 cut(s) 680, 1022
AluBI AGCT 6 cut(s) 67, 103, 136, 674, 905, 1007
AluI AGCT 6 cut(s) 67, 103, 136, 674, 905, 1007
Alw21I GWGCWC 1 cut(s) 762
Alw26I GTCTC 2 cut(s) 97, 195
AlwI GGATC 5 cut(s) 18, 558, 571, 934, 947
AoxI GGCC 2 cut(s) 685, 1020
ApeKI GCWGC 1 cut(s) 103
ArsI GACNNNNNNTTYG 2 cut(s) 275, 307
AseI ATTAAT 3 cut(s) 645, 765, 789
Asp700I GAANNNNTTC 1 cut(s) 344
AspLEI GCGC 1 cut(s) 882
AspS9I GGNCC 4 cut(s) 45, 664, 685, 949
AsuHPI GGTGA 1 cut(s) 671
AvaII GGWCC 3 cut(s) 45, 664, 949
BamHI GGATCC 2 cut(s) 563, 939
BbsI GAAGAC 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 762
BbvI GCAGC 1 cut(s) 90
BccI CCATC 2 cut(s) 554, 804
BciT130I CCWGG 2 cut(s) 682, 1024
BclI TGATCA 2 cut(s) 813, 963
BcoDI GTCTC 2 cut(s) 97, 195
BfaI CTAG 4 cut(s) 707, 902, 1008, 1036
BfmI CTRYAG 2 cut(s) 823, 944
BfuAI ACCTGC 4 cut(s) 211, 538, 584, 647
BisI GCNGC 1 cut(s) 104
BlpI GCTNAGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 105
Bme1390I CCNGG 2 cut(s) 682, 1024
Bme18I GGWCC 3 cut(s) 45, 664, 949
BmgT120I GGNCC 4 cut(s) 45, 664, 685, 949
BmiI GGNNCC 6 cut(s) 47, 565, 686, 941, 951, 999
BmrFI CCNGG 2 cut(s) 682, 1024
BmrI ACTGGG 1 cut(s) 627
BmsI GCATC 2 cut(s) 5, 825
BmuI ACTGGG 1 cut(s) 627
BpiI GAAGAC 1 cut(s) 288
Bpu10I CCTNAGC 1 cut(s) 63
Bpu1102I GCTNAGC 1 cut(s) 216
BpuEI CTTGAG 1 cut(s) 79
Bsa29I ATCGAT 1 cut(s) 13
BsaJI CCNNGG 5 cut(s) 210, 518, 681, 801, 1023
Bsc4I CCNNNNNNNGG 2 cut(s) 536, 802
Bse1I ACTGG 6 cut(s) 47, 157, 290, 341, 541, 622
Bse3DI GCAATG 1 cut(s) 583
BseBI CCWGG 2 cut(s) 682, 1024
BseCI ATCGAT 1 cut(s) 13
BseDI CCNNGG 5 cut(s) 210, 518, 681, 801, 1023
BseGI GGATG 4 cut(s) 140, 796, 852, 988
BseLI CCNNNNNNNGG 2 cut(s) 536, 802
BseMI GCAATG 1 cut(s) 583
BseMII CTCAG 1 cut(s) 666
BseNI ACTGG 6 cut(s) 47, 157, 290, 341, 541, 622
BseRI GAGGAG 1 cut(s) 742
BseXI GCAGC 1 cut(s) 90
BshFI GGCC 2 cut(s) 687, 1022
BshVI ATCGAT 1 cut(s) 13
BsiHKAI GWGCWC 1 cut(s) 762
BslFI GGGAC 2 cut(s) 31, 192
BslI CCNNNNNNNGG 2 cut(s) 536, 802
BsmAI GTCTC 2 cut(s) 97, 195
BsmFI GGGAC 2 cut(s) 31, 192
BsmI GAATGC 2 cut(s) 229, 977
BsnI GGCC 2 cut(s) 687, 1022
Bsp1286I GDGCHC 1 cut(s) 762
Bsp143I GATC 6 cut(s) 10, 563, 813, 895, 939, 963
Bsp1720I GCTNAGC 1 cut(s) 216
BspANI GGCC 2 cut(s) 687, 1022
BspCNI CTCAG 1 cut(s) 667
BspDI ATCGAT 1 cut(s) 13
BspHI TCATGA 1 cut(s) 816
BspLI GGNNCC 6 cut(s) 47, 565, 686, 941, 951, 999
BspMAI CTGCAG 1 cut(s) 948
BspMI ACCTGC 4 cut(s) 211, 538, 584, 647
BspPI GGATC 5 cut(s) 18, 558, 571, 934, 947
BsrDI GCAATG 1 cut(s) 583
BsrI ACTGG 6 cut(s) 47, 157, 290, 341, 541, 622
BssECI CCNNGG 5 cut(s) 210, 518, 681, 801, 1023
BssMI GATC 6 cut(s) 10, 563, 813, 895, 939, 963
BssT1I CCWWGG 3 cut(s) 210, 518, 801
Bst2UI CCWGG 2 cut(s) 682, 1024
Bst4CI ACNGT 3 cut(s) 206, 824, 914
BstC8I GCNNGC 4 cut(s) 101, 574, 924, 1033
BstDEI CTNAG 6 cut(s) 63, 216, 477, 524, 675, 906
BstF5I GGATG 4 cut(s) 140, 796, 852, 988
BstHHI GCGC 1 cut(s) 882
BstKTI GATC 6 cut(s) 13, 566, 816, 898, 942, 966
BstMAI GTCTC 2 cut(s) 97, 195
BstMBI GATC 6 cut(s) 10, 563, 813, 895, 939, 963
BstMWI GCNNNNNNNGC 7 cut(s) 162, 226, 245, 520, 590, 871, 1028
BstNI CCWGG 2 cut(s) 682, 1024
BstNSI RCATGY 2 cut(s) 256, 926
BstSCI CCNGG 2 cut(s) 680, 1022
BstSFI CTRYAG 2 cut(s) 823, 944
BstV1I GCAGC 1 cut(s) 90
BstV2I GAAGAC 1 cut(s) 288
BstX2I RGATCY 2 cut(s) 563, 939
BstXI CCANNNNNNTGG 1 cut(s) 26
BstYI RGATCY 2 cut(s) 563, 939
Bsu15I ATCGAT 1 cut(s) 13
BsuRI GGCC 2 cut(s) 687, 1022
BsuTUI ATCGAT 1 cut(s) 13
BtsCI GGATG 4 cut(s) 140, 796, 852, 988
BtsIMutI CAGTG 2 cut(s) 615, 966
BveI ACCTGC 4 cut(s) 211, 538, 584, 647
Cac8I GCNNGC 4 cut(s) 101, 574, 924, 1033
CciI TCATGA 1 cut(s) 816
CfoI GCGC 1 cut(s) 882
Cfr13I GGNCC 4 cut(s) 45, 664, 685, 949
ClaI ATCGAT 1 cut(s) 13
CviAII CATG 7 cut(s) 253, 322, 695, 817, 839, 886, 923
DdeI CTNAG 6 cut(s) 63, 216, 477, 524, 675, 906
DpnI GATC 6 cut(s) 12, 565, 815, 897, 941, 965
DpnII GATC 6 cut(s) 10, 563, 813, 895, 939, 963
DraI TTTAAA 2 cut(s) 127, 273
Eco130I CCWWGG 3 cut(s) 210, 518, 801
Eco47I GGWCC 3 cut(s) 45, 664, 949
Eco57I CTGAAG 1 cut(s) 453
EcoO109I RGGNCCY 3 cut(s) 664, 685, 949
EcoRII CCWGG 2 cut(s) 680, 1022
EcoT14I CCWWGG 3 cut(s) 210, 518, 801
EcoT22I ATGCAT 2 cut(s) 777, 840
ErhI CCWWGG 3 cut(s) 210, 518, 801
FaeI CATG 7 cut(s) 256, 325, 698, 820, 842, 889, 926
FaqI GGGAC 2 cut(s) 31, 192
FatI CATG 7 cut(s) 252, 321, 694, 816, 838, 885, 922
FbaI TGATCA 2 cut(s) 813, 963
FblI GTMKAC 1 cut(s) 826
Fnu4HI GCNGC 1 cut(s) 104
FokI GGATG 4 cut(s) 127, 783, 839, 995
Fsp4HI GCNGC 1 cut(s) 104
FspBI CTAG 4 cut(s) 707, 902, 1008, 1036
GlaI GCGC 1 cut(s) 881
GluI GCNGC 1 cut(s) 104
HaeIII GGCC 2 cut(s) 687, 1022
HhaI GCGC 1 cut(s) 882
Hin1II CATG 7 cut(s) 256, 325, 698, 820, 842, 889, 926
Hin6I GCGC 1 cut(s) 880
HinP1I GCGC 1 cut(s) 880
HinfI GANTC 4 cut(s) 677, 743, 749, 933
HphI GGTGA 1 cut(s) 671
Hpy166II GTNNAC 1 cut(s) 827
Hpy188I TCNGA 2 cut(s) 433, 748
Hpy188III TCNNGA 5 cut(s) 200, 567, 817, 856, 937
Hpy8I GTNNAC 1 cut(s) 827
HpyAV CCTTC 3 cut(s) 59, 159, 677
HpyCH4III ACNGT 3 cut(s) 206, 824, 914
HpyCH4IV ACGT 1 cut(s) 809
HpyCH4V TGCA 8 cut(s) 165, 307, 387, 703, 775, 838, 922, 946
HpyF10VI GCNNNNNNNGC 7 cut(s) 162, 226, 245, 520, 590, 871, 1028
HpyF3I CTNAG 6 cut(s) 63, 216, 477, 524, 675, 906
HpySE526I ACGT 1 cut(s) 809
Hsp92II CATG 7 cut(s) 256, 325, 698, 820, 842, 889, 926
HspAI GCGC 1 cut(s) 880
Ksp22I TGATCA 2 cut(s) 813, 963
Kzo9I GATC 6 cut(s) 10, 563, 813, 895, 939, 963
LmnI GCTCC 1 cut(s) 590
Lsp1109I GCAGC 1 cut(s) 90
LweI GCATC 2 cut(s) 5, 825
MaeI CTAG 4 cut(s) 707, 902, 1008, 1036
MaeII ACGT 1 cut(s) 809
MaeIII GTNAC 3 cut(s) 186, 724, 779
MalI GATC 6 cut(s) 12, 565, 815, 897, 941, 965
MboI GATC 6 cut(s) 10, 563, 813, 895, 939, 963
MboII GAAGA 3 cut(s) 293, 340, 346
MflI RGATCY 2 cut(s) 563, 939
MhlI GDGCHC 1 cut(s) 762
MluCI AATT 5 cut(s) 268, 274, 472, 731, 990
MlyI GAGTC 2 cut(s) 686, 743
MnlI CCTC 8 cut(s) 30, 69, 375, 439, 655, 698, 763, 1004
Mph1103I ATGCAT 2 cut(s) 777, 840
MroXI GAANNNNTTC 1 cut(s) 344
MspR9I CCNGG 2 cut(s) 682, 1024
Mva1269I GAATGC 2 cut(s) 229, 977
MvaI CCWGG 2 cut(s) 682, 1024
MwoI GCNNNNNNNGC 7 cut(s) 162, 226, 245, 520, 590, 871, 1028
NdeII GATC 6 cut(s) 10, 563, 813, 895, 939, 963
NlaIII CATG 7 cut(s) 256, 325, 698, 820, 842, 889, 926
NlaIV GGNNCC 6 cut(s) 47, 565, 686, 941, 951, 999
NmuCI GTSAC 1 cut(s) 779
NsiI ATGCAT 2 cut(s) 777, 840
NspI RCATGY 2 cut(s) 256, 926
PaeI GCATGC 1 cut(s) 926
PagI TCATGA 1 cut(s) 816
PaqCI CACCTGC 2 cut(s) 584, 647
PcsI WCGNNNNNNNCGW 1 cut(s) 127
PctI GAATGC 2 cut(s) 229, 977
PdmI GAANNNNTTC 1 cut(s) 344
PfeI GAWTC 2 cut(s) 743, 933
PflMI CCANNNNNTGG 1 cut(s) 802
PkrI GCNGC 1 cut(s) 105
PleI GAGTC 2 cut(s) 685, 743
PpsI GAGTC 2 cut(s) 685, 743
PpuMI RGGWCCY 2 cut(s) 664, 949
PshBI ATTAAT 3 cut(s) 645, 765, 789
PsiI TTATAA 1 cut(s) 605
Psp1406I AACGTT 1 cut(s) 809
Psp5II RGGWCCY 2 cut(s) 664, 949
Psp6I CCWGG 2 cut(s) 680, 1022
PspGI CCWGG 2 cut(s) 680, 1022
PspN4I GGNNCC 6 cut(s) 47, 565, 686, 941, 951, 999
PspPI GGNCC 4 cut(s) 45, 664, 685, 949
PspPPI RGGWCCY 2 cut(s) 664, 949
PstI CTGCAG 1 cut(s) 948
PsuI RGATCY 2 cut(s) 563, 939
SatI GCNGC 1 cut(s) 104
Sau3AI GATC 6 cut(s) 10, 563, 813, 895, 939, 963
Sau96I GGNCC 4 cut(s) 45, 664, 685, 949
SbfI CCTGCAGG 1 cut(s) 948
SchI GAGTC 2 cut(s) 686, 743
ScrFI CCNGG 2 cut(s) 682, 1024
SdaI CCTGCAGG 1 cut(s) 948
SduI GDGCHC 1 cut(s) 762
SfaNI GCATC 2 cut(s) 5, 825
SfcI CTRYAG 2 cut(s) 823, 944
SinI GGWCC 3 cut(s) 45, 664, 949
SmiI ATTTAAAT 1 cut(s) 273
SmlI CTYRAG 1 cut(s) 94
SmoI CTYRAG 1 cut(s) 94
SphI GCATGC 1 cut(s) 926
Sse8387I CCTGCAGG 1 cut(s) 948
Sse9I AATT 5 cut(s) 268, 274, 472, 731, 990
SspI AATATT 3 cut(s) 75, 343, 787
SspMI CTAG 4 cut(s) 707, 902, 1008, 1036
StyD4I CCNGG 2 cut(s) 680, 1022
StyI CCWWGG 3 cut(s) 210, 518, 801
SwaI ATTTAAAT 1 cut(s) 273
TaaI ACNGT 3 cut(s) 206, 824, 914
TaiI ACGT 1 cut(s) 812
TaqI TCGA 2 cut(s) 13, 199
TasI AATT 5 cut(s) 268, 274, 472, 731, 990
TfiI GAWTC 2 cut(s) 743, 933
TscAI CASTG 2 cut(s) 622, 973
TseFI GTSAC 1 cut(s) 779
TseI GCWGC 1 cut(s) 103
Tsp45I GTSAC 1 cut(s) 779
TspDTI ATGAA 6 cut(s) 17, 129, 338, 340, 833, 841
TspGWI ACGGA 1 cut(s) 401
TspRI CASTG 2 cut(s) 622, 973
Van91I CCANNNNNTGG 1 cut(s) 802
VpaK11BI GGWCC 3 cut(s) 45, 664, 949
VspI ATTAAT 3 cut(s) 645, 765, 789
XceI RCATGY 2 cut(s) 256, 926
XmiI GTMKAC 1 cut(s) 826
XmnI GAANNNNTTC 1 cut(s) 344
XspI CTAG 4 cut(s) 707, 902, 1008, 1036
Zsp2I ATGCAT 2 cut(s) 777, 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.