Rh1CG231100

Methionine adenosyltransferase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0001020
Physical Location & Seq
Forward (+)
48581495 .. 48584770
3276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG231100.1

Sequence Viewer

Length: 672 bp
ATGGATCCTGCTGCAGCTATGTCAGTTAATGTGCCATCTTCTATTGTTAATTGGTTATCGAGCTTAGAAGAGAGTAATTATCTTCTGATCCATTTGTCAACTGATCAAGTTTATGAAGGGGTGAAGTCCTTTTACAAGGAAGATGATGAAGTTGTTCCAGTAAATGTTTATGGGAAATCAAAAGTGGCAGCTGAGCAGTTCATTACTGAGAAATGCTCAAACTTTGCAATTTTGAGAAGCAGTATCATCTTTGGGCCACAGACAATCTCACCAGTTTCAAAATCTCTTCCGATTCAGTGGGTTGATGGTGTCCTCTCCAAAGGAAATACAACCGAATTCTTTCATGATGAGTTTCGATGCCCTGTGTATGTAAAGGATGTTGTAGCAATCATACTTGCTTTGTCCGAGACATGGATATCAGAGGCTAAGCAAAGAAAATTGCTACTGAATGTTGGTGGACCAGACAGGGTATCCCGTGTACAAATGGCTGAGACGGTTGCTGATATAAGGGGATACAACCTCTCATTAATTAAATCTGTATCTGCATCATCGGTTGATCGTGGAGTTATGTCTCCTGCTGACATATCCATGGATATAACTAAGCTAGTTCAGAGTCTTGGTATTTCTCCTATTTCATTTCGAGATGGTGTCAGATTGACGCTTGAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RmlD_sub_bind

Protein Analysis

223

Amino Acids

24.52

Weight (kDa)

5.13

Isoelectric Point (pI)

39.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 4 - 221 1.3e-29 RmlD substrate binding domain
Epimerase PF01370 19 - 137 3.8e-08 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 12, 82
AcsI RAATTY 1 cut(s) 335
AfaI GTAC 1 cut(s) 480
AfiI CCNNNNNNNGG 1 cut(s) 411
AgsI TTSAA 2 cut(s) 279, 665
AluBI AGCT 4 cut(s) 17, 63, 191, 604
AluI AGCT 4 cut(s) 17, 63, 191, 604
Alw26I GTCTC 3 cut(s) 401, 485, 576
AlwI GGATC 2 cut(s) 12, 82
AoxI GGCC 1 cut(s) 254
ApeKI GCWGC 3 cut(s) 11, 14, 188
ApoI RAATTY 1 cut(s) 335
AseI ATTAAT 1 cut(s) 527
Asp700I GAANNNNTTC 2 cut(s) 153, 339
AspS9I GGNCC 2 cut(s) 254, 458
AsuHPI GGTGA 2 cut(s) 133, 261
AvaII GGWCC 1 cut(s) 458
BamHI GGATCC 1 cut(s) 4
BarI GAAGNNNNNNTAC 2 cut(s) 116, 148
BbvI GCAGC 2 cut(s) 26, 200
BccI CCATC 3 cut(s) 43, 299, 638
BciVI GTATCC 2 cut(s) 481, 506
BclI TGATCA 1 cut(s) 103
BcoDI GTCTC 3 cut(s) 401, 485, 576
BfaI CTAG 1 cut(s) 605
BfmI CTRYAG 1 cut(s) 12
BfuI GTATCC 2 cut(s) 481, 506
BisI GCNGC 3 cut(s) 12, 15, 189
BlpI GCTNAGC 2 cut(s) 192, 426
BlsI GCNGC 3 cut(s) 13, 16, 190
Bme18I GGWCC 1 cut(s) 458
BmgT120I GGNCC 2 cut(s) 254, 458
BmiI GGNNCC 1 cut(s) 6
BmsI GCATC 2 cut(s) 347, 554
BplI GAGNNNNNCTC 2 cut(s) 200, 232
Bpu1102I GCTNAGC 2 cut(s) 192, 426
BsaJI CCNNGG 1 cut(s) 588
Bsc4I CCNNNNNNNGG 1 cut(s) 411
Bse1I ACTGG 2 cut(s) 158, 272
BseDI CCNNGG 1 cut(s) 588
BseGI GGATG 1 cut(s) 382
BseLI CCNNNNNNNGG 1 cut(s) 411
BseMII CTCAG 3 cut(s) 183, 198, 480
BseNI ACTGG 2 cut(s) 158, 272
BseXI GCAGC 2 cut(s) 26, 200
BshFI GGCC 1 cut(s) 256
BslI CCNNNNNNNGG 1 cut(s) 411
BsmAI GTCTC 3 cut(s) 401, 485, 576
BsmBI CGTCTC 1 cut(s) 485
BsnI GGCC 1 cut(s) 256
Bsp1407I TGTACA 1 cut(s) 478
Bsp143I GATC 4 cut(s) 4, 87, 103, 556
Bsp1720I GCTNAGC 2 cut(s) 192, 426
Bsp19I CCATGG 1 cut(s) 588
BspANI GGCC 1 cut(s) 256
BspCNI CTCAG 3 cut(s) 184, 199, 481
BspHI TCATGA 1 cut(s) 343
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 16
BspPI GGATC 2 cut(s) 12, 82
BsrGI TGTACA 1 cut(s) 478
BsrI ACTGG 2 cut(s) 158, 272
BssECI CCNNGG 1 cut(s) 588
BssMI GATC 4 cut(s) 4, 87, 103, 556
BssT1I CCWWGG 1 cut(s) 588
Bst4CI ACNGT 2 cut(s) 496, 669
Bst6I CTCTTC 2 cut(s) 63, 291
BstAUI TGTACA 1 cut(s) 478
BstDEI CTNAG 6 cut(s) 64, 192, 207, 426, 489, 600
BstDSI CCRYGG 1 cut(s) 588
BstF5I GGATG 1 cut(s) 382
BstKTI GATC 4 cut(s) 7, 90, 106, 559
BstMAI GTCTC 3 cut(s) 401, 485, 576
BstMBI GATC 4 cut(s) 4, 87, 103, 556
BstSFI CTRYAG 1 cut(s) 12
BstV1I GCAGC 2 cut(s) 26, 200
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuI GTATCC 2 cut(s) 481, 506
BsuRI GGCC 1 cut(s) 256
BtgI CCRYGG 1 cut(s) 588
BtsCI GGATG 1 cut(s) 382
BtsIMutI CAGTG 1 cut(s) 302
CciI TCATGA 1 cut(s) 343
Cfr13I GGNCC 2 cut(s) 254, 458
CseI GACGC 1 cut(s) 667
Csp6I GTAC 1 cut(s) 479
CviAII CATG 3 cut(s) 344, 411, 589
CviJI RGCY 7 cut(s) 17, 63, 191, 256, 425, 488, 604
CviKI_1 RGCY 7 cut(s) 17, 63, 191, 256, 425, 488, 604
CviQI GTAC 1 cut(s) 479
DdeI CTNAG 6 cut(s) 64, 192, 207, 426, 489, 600
DpnI GATC 4 cut(s) 6, 89, 105, 558
DpnII GATC 4 cut(s) 4, 87, 103, 556
Eam1104I CTCTTC 2 cut(s) 63, 291
EarI CTCTTC 2 cut(s) 63, 291
Eco130I CCWWGG 1 cut(s) 588
Eco32I GATATC 1 cut(s) 417
Eco47I GGWCC 1 cut(s) 458
EcoRI GAATTC 1 cut(s) 335
EcoRV GATATC 1 cut(s) 417
EcoT14I CCWWGG 1 cut(s) 588
ErhI CCWWGG 1 cut(s) 588
Esp3I CGTCTC 1 cut(s) 485
FaeI CATG 3 cut(s) 347, 414, 592
FatI CATG 3 cut(s) 343, 410, 588
FbaI TGATCA 1 cut(s) 103
Fnu4HI GCNGC 3 cut(s) 12, 15, 189
FokI GGATG 1 cut(s) 389
Fsp4HI GCNGC 3 cut(s) 12, 15, 189
FspBI CTAG 1 cut(s) 605
GluI GCNGC 3 cut(s) 12, 15, 189
HaeIII GGCC 1 cut(s) 256
HgaI GACGC 1 cut(s) 667
Hin1II CATG 3 cut(s) 347, 414, 592
HincII GTYRAC 1 cut(s) 99
HindII GTYRAC 1 cut(s) 99
HinfI GANTC 2 cut(s) 292, 613
HphI GGTGA 2 cut(s) 133, 261
Hpy166II GTNNAC 3 cut(s) 99, 458, 479
Hpy188I TCNGA 6 cut(s) 87, 291, 406, 421, 612, 653
Hpy188III TCNNGA 2 cut(s) 344, 641
Hpy8I GTNNAC 3 cut(s) 99, 458, 479
HpyAV CCTTC 1 cut(s) 110
HpyCH4III ACNGT 2 cut(s) 496, 669
HpyCH4V TGCA 3 cut(s) 14, 227, 545
HpyF3I CTNAG 6 cut(s) 64, 192, 207, 426, 489, 600
Hsp92II CATG 3 cut(s) 347, 414, 592
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 4 cut(s) 4, 87, 103, 556
LpnPI CCDG 7 cut(s) 21, 171, 285, 375, 451, 474, 588
Lsp1109I GCAGC 2 cut(s) 26, 200
LweI GCATC 2 cut(s) 347, 554
MaeI CTAG 1 cut(s) 605
MalI GATC 4 cut(s) 6, 89, 105, 558
MboI GATC 4 cut(s) 4, 87, 103, 556
MboII GAAGA 5 cut(s) 30, 74, 80, 152, 278
MflI RGATCY 1 cut(s) 4
MluCI AATT 6 cut(s) 49, 76, 228, 335, 437, 528
MlyI GAGTC 1 cut(s) 622
MnlI CCTC 3 cut(s) 323, 415, 530
MroXI GAANNNNTTC 2 cut(s) 153, 339
MseI TTAA 4 cut(s) 27, 48, 527, 531
MslI CAYNNNNRTG 1 cut(s) 587
MspA1I CMGCKG 1 cut(s) 191
NcoI CCATGG 1 cut(s) 588
NdeII GATC 4 cut(s) 4, 87, 103, 556
NlaIII CATG 3 cut(s) 347, 414, 592
NlaIV GGNNCC 1 cut(s) 6
PacI TTAATTAA 1 cut(s) 531
PagI TCATGA 1 cut(s) 343
PdmI GAANNNNTTC 2 cut(s) 153, 339
PfeI GAWTC 1 cut(s) 292
PkrI GCNGC 3 cut(s) 13, 16, 190
PleI GAGTC 1 cut(s) 621
PpsI GAGTC 1 cut(s) 621
PshBI ATTAAT 1 cut(s) 527
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 254, 458
PstI CTGCAG 1 cut(s) 16
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 191
RsaI GTAC 1 cut(s) 480
RsaNI GTAC 1 cut(s) 479
RseI CAYNNNNRTG 1 cut(s) 587
SaqAI TTAA 4 cut(s) 27, 48, 527, 531
SatI GCNGC 3 cut(s) 12, 15, 189
Sau3AI GATC 4 cut(s) 4, 87, 103, 556
Sau96I GGNCC 2 cut(s) 254, 458
SchI GAGTC 1 cut(s) 622
SetI ASST 5 cut(s) 19, 65, 193, 522, 606
SfaNI GCATC 2 cut(s) 347, 554
SfcI CTRYAG 1 cut(s) 12
SinI GGWCC 1 cut(s) 458
SmiMI CAYNNNNRTG 1 cut(s) 587
Sse9I AATT 6 cut(s) 49, 76, 228, 335, 437, 528
SspMI CTAG 1 cut(s) 605
StyI CCWWGG 1 cut(s) 588
TaaI ACNGT 2 cut(s) 496, 669
TaqI TCGA 3 cut(s) 59, 355, 640
TasI AATT 6 cut(s) 49, 76, 228, 335, 437, 528
TatI WGTACW 1 cut(s) 478
TfiI GAWTC 1 cut(s) 292
Tru1I TTAA 4 cut(s) 27, 48, 527, 531
Tru9I TTAA 4 cut(s) 27, 48, 527, 531
TscAI CASTG 1 cut(s) 302
TseI GCWGC 3 cut(s) 11, 14, 188
TspDTI ATGAA 5 cut(s) 129, 162, 190, 332, 624
TspRI CASTG 1 cut(s) 302
VpaK11BI GGWCC 1 cut(s) 458
VspI ATTAAT 1 cut(s) 527
XapI RAATTY 1 cut(s) 335
XmnI GAANNNNTTC 2 cut(s) 153, 339
XspI CTAG 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.