pycom01g07780

Methionine adenosyltransferase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001020
Physical Location & Seq
Forward (+)
8779766 .. 8781740
1975 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g07780.3

Sequence Viewer

Length: 606 bp
ATGACAAGTAAGAAGAGAGTGCTGGTAGTTAGAGGCACAGGTTACTTGGGCCAGCATGTACTGCAAGAGTTCTCAGAGATTGAAGGGATCACAGCAACCACGCCTTTCGGTCTGGCTTTTACCCACCACTCCAGTCCTCCTCCCCAAGCATTGCTCGATGCATTTCCCCAGTTGCTGCATTTCCATGTCGATTTGCAGACCGGCCATGGATTTCAAGCCATTTCTCGGACGTTTGGCCCGCCTGATGTGGTTGTAAACTGCGCTGCACTCTCCGTTCCTCGTGCCTGTGAAATGGATCCTGCTGCTGCTATGTCTGTGAATGTTCCATCTTCTCTTGTGAATTGGTTATCAAGCTTTGAAGAGAGTAGTTCTCTTCTGATACATTTGTCAACTGATCAAGGGGTGAAGTCCTTTTACAAGGAAGATGATGAAACCGCTCCTGTAAATGTATATGGGAAATCGAAAGTGGCAGCAGAGCAGTTCATAACTGAAAAATGCTCCAACTTTGCAATTTTGAGAAGCAGTATCATCTTTGGGCCACAGACAATCTCACTGGTTTCAAAATCTCTTCCAATCCAGGTATACAGAAGACAGTCCTTCGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RmlD_sub_bind

Protein Analysis

202

Amino Acids

21.9

Weight (kDa)

6.37

Isoelectric Point (pI)

56.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 437
AccI GTMKAC 1 cut(s) 582
AciI CCGC 2 cut(s) 239, 435
AclWI GGATC 3 cut(s) 95, 290, 303
AcoI YGGCCR 1 cut(s) 202
AfaI GTAC 1 cut(s) 60
AfiI CCNNNNNNNGG 1 cut(s) 225
AgsI TTSAA 4 cut(s) 83, 215, 359, 561
AjnI CCWGG 1 cut(s) 576
AluBI AGCT 1 cut(s) 354
AluI AGCT 1 cut(s) 354
AlwI GGATC 3 cut(s) 95, 290, 303
AlwNI CAGNNNCTG 1 cut(s) 175
AoxI GGCC 4 cut(s) 49, 202, 235, 536
ApeKI GCWGC 5 cut(s) 175, 263, 302, 305, 470
AspLEI GCGC 1 cut(s) 263
AspS9I GGNCC 3 cut(s) 49, 236, 536
AsuHPI GGTGA 1 cut(s) 415
BaeI ACNNNNGTAYC 2 cut(s) 371, 404
BamHI GGATCC 1 cut(s) 295
BarI GAAGNNNNNNTAC 2 cut(s) 398, 430
BauI CACGAG 1 cut(s) 279
BbsI GAAGAC 1 cut(s) 595
BbvI GCAGC 5 cut(s) 162, 250, 289, 292, 482
BccI CCATC 1 cut(s) 334
BciT130I CCWGG 1 cut(s) 578
BclI TGATCA 1 cut(s) 394
BisI GCNGC 5 cut(s) 176, 264, 303, 306, 471
BlsI GCNGC 5 cut(s) 177, 265, 304, 307, 472
Bme1390I CCNGG 1 cut(s) 578
BmgT120I GGNCC 3 cut(s) 49, 236, 536
BmiI GGNNCC 1 cut(s) 297
BmrFI CCNGG 1 cut(s) 578
BmrI ACTGGG 1 cut(s) 163
BmsI GCATC 1 cut(s) 148
BmuI ACTGGG 1 cut(s) 163
BpiI GAAGAC 1 cut(s) 595
BplI GAGNNNNNCTC 2 cut(s) 355, 387
BpmI CTGGAG 1 cut(s) 115
BsaJI CCNNGG 1 cut(s) 205
Bsc4I CCNNNNNNNGG 1 cut(s) 225
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 3 cut(s) 132, 169, 558
Bse3DI GCAATG 1 cut(s) 149
BseBI CCWGG 1 cut(s) 578
BseDI CCNNGG 1 cut(s) 205
BseLI CCNNNNNNNGG 1 cut(s) 225
BseMI GCAATG 1 cut(s) 149
BseMII CTCAG 1 cut(s) 87
BseNI ACTGG 3 cut(s) 132, 169, 558
BseRI GAGGAG 1 cut(s) 129
BseXI GCAGC 5 cut(s) 162, 250, 289, 292, 482
BsgI GTGCAG 1 cut(s) 249
BshFI GGCC 4 cut(s) 51, 204, 237, 538
BsiSI CCGG 1 cut(s) 201
BslI CCNNNNNNNGG 1 cut(s) 225
BsnI GGCC 4 cut(s) 51, 204, 237, 538
Bsp143I GATC 3 cut(s) 87, 295, 394
Bsp19I CCATGG 1 cut(s) 205
BspACI CCGC 2 cut(s) 239, 435
BspANI GGCC 4 cut(s) 51, 204, 237, 538
BspCNI CTCAG 1 cut(s) 86
BspLI GGNNCC 1 cut(s) 297
BspPI GGATC 3 cut(s) 95, 290, 303
BsrBI CCGCTC 1 cut(s) 437
BsrDI GCAATG 1 cut(s) 149
BsrFI RCCGGY 1 cut(s) 200
BsrI ACTGG 3 cut(s) 132, 169, 558
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 3 cut(s) 87, 295, 394
BssNAI GTATAC 1 cut(s) 583
BssSI CACGAG 1 cut(s) 279
BssT1I CCWWGG 1 cut(s) 205
Bst1107I GTATAC 1 cut(s) 583
Bst2BI CACGAG 1 cut(s) 279
Bst2UI CCWGG 1 cut(s) 578
Bst4CI ACNGT 1 cut(s) 594
Bst6I CTCTTC 4 cut(s) 8, 354, 378, 573
BstAPI GCANNNNNTGC 1 cut(s) 61
BstC8I GCNNGC 2 cut(s) 53, 239
BstDEI CTNAG 1 cut(s) 73
BstDSI CCRYGG 1 cut(s) 205
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 3 cut(s) 90, 298, 397
BstMBI GATC 3 cut(s) 87, 295, 394
BstMWI GCNNNNNNNGC 1 cut(s) 61
BstNI CCWGG 1 cut(s) 578
BstNSI RCATGY 1 cut(s) 59
BstSCI CCNGG 1 cut(s) 576
BstV1I GCAGC 5 cut(s) 162, 250, 289, 292, 482
BstV2I GAAGAC 1 cut(s) 595
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
BstZ17I GTATAC 1 cut(s) 583
BsuRI GGCC 4 cut(s) 51, 204, 237, 538
BtgI CCRYGG 1 cut(s) 205
BtsIMutI CAGTG 1 cut(s) 551
Cac8I GCNNGC 2 cut(s) 53, 239
CaiI CAGNNNCTG 1 cut(s) 175
CfoI GCGC 1 cut(s) 263
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 3 cut(s) 49, 236, 536
Csp6I GTAC 1 cut(s) 59
CviAII CATG 3 cut(s) 56, 185, 206
CviJI RGCY 7 cut(s) 51, 116, 204, 218, 237, 354, 538
CviKI_1 RGCY 7 cut(s) 51, 116, 204, 218, 237, 354, 538
CviQI GTAC 1 cut(s) 59
DdeI CTNAG 1 cut(s) 73
DpnI GATC 3 cut(s) 89, 297, 396
DpnII GATC 3 cut(s) 87, 295, 394
EaeI YGGCCR 1 cut(s) 202
Eam1104I CTCTTC 4 cut(s) 8, 354, 378, 573
EarI CTCTTC 4 cut(s) 8, 354, 378, 573
Eco130I CCWWGG 1 cut(s) 205
EcoRII CCWGG 1 cut(s) 576
EcoT14I CCWWGG 1 cut(s) 205
EcoT22I ATGCAT 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 205
FaeI CATG 3 cut(s) 59, 188, 209
FaiI YATR 8 cut(s) 57, 186, 207, 311, 451, 453, 485, 583
FatI CATG 3 cut(s) 55, 184, 205
FauI CCCGC 1 cut(s) 246
FbaI TGATCA 1 cut(s) 394
FblI GTMKAC 1 cut(s) 582
Fnu4HI GCNGC 5 cut(s) 176, 264, 303, 306, 471
Fsp4HI GCNGC 5 cut(s) 176, 264, 303, 306, 471
GlaI GCGC 1 cut(s) 262
GluI GCNGC 5 cut(s) 176, 264, 303, 306, 471
GsuI CTGGAG 1 cut(s) 115
HaeIII GGCC 4 cut(s) 51, 204, 237, 538
HapII CCGG 1 cut(s) 201
HhaI GCGC 1 cut(s) 263
Hin1II CATG 3 cut(s) 59, 188, 209
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HincII GTYRAC 1 cut(s) 390
HindII GTYRAC 1 cut(s) 390
HindIII AAGCTT 1 cut(s) 352
HpaII CCGG 1 cut(s) 201
HphI GGTGA 1 cut(s) 415
Hpy166II GTNNAC 3 cut(s) 256, 390, 583
Hpy188I TCNGA 3 cut(s) 76, 228, 378
Hpy8I GTNNAC 3 cut(s) 256, 390, 583
HpyAV CCTTC 1 cut(s) 77
HpyCH4III ACNGT 1 cut(s) 594
HpyCH4IV ACGT 1 cut(s) 230
HpyCH4V TGCA 6 cut(s) 64, 161, 178, 196, 266, 509
HpyF10VI GCNNNNNNNGC 1 cut(s) 61
HpyF3I CTNAG 1 cut(s) 73
HpySE526I ACGT 1 cut(s) 230
Hsp92II CATG 3 cut(s) 59, 188, 209
HspAI GCGC 1 cut(s) 261
Ksp22I TGATCA 1 cut(s) 394
Kzo9I GATC 3 cut(s) 87, 295, 394
LmnI GCTCC 2 cut(s) 442, 503
Lsp1109I GCAGC 5 cut(s) 162, 250, 289, 292, 482
LweI GCATC 1 cut(s) 148
MaeII ACGT 1 cut(s) 230
MaeIII GTNAC 1 cut(s) 41
MalI GATC 3 cut(s) 89, 297, 396
MbiI CCGCTC 1 cut(s) 437
MboI GATC 3 cut(s) 87, 295, 394
MboII GAAGA 7 cut(s) 25, 321, 365, 371, 434, 560, 600
MflI RGATCY 1 cut(s) 295
MluCI AATT 2 cut(s) 340, 510
MmeI TCCRAC 1 cut(s) 525
MnlI CCTC 4 cut(s) 26, 147, 150, 288
Mph1103I ATGCAT 1 cut(s) 163
MslI CAYNNNNRTG 1 cut(s) 183
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 1 cut(s) 578
MvaI CCWGG 1 cut(s) 578
MwoI GCNNNNNNNGC 1 cut(s) 61
NcoI CCATGG 1 cut(s) 205
NdeII GATC 3 cut(s) 87, 295, 394
NlaIII CATG 3 cut(s) 59, 188, 209
NlaIV GGNNCC 1 cut(s) 297
NsiI ATGCAT 1 cut(s) 163
NspI RCATGY 1 cut(s) 59
PkrI GCNGC 5 cut(s) 177, 265, 304, 307, 472
Psp6I CCWGG 1 cut(s) 576
PspGI CCWGG 1 cut(s) 576
PspN4I GGNNCC 1 cut(s) 297
PspPI GGNCC 3 cut(s) 49, 236, 536
PstNI CAGNNNCTG 1 cut(s) 175
PsuI RGATCY 1 cut(s) 295
RsaI GTAC 1 cut(s) 60
RsaNI GTAC 1 cut(s) 59
RseI CAYNNNNRTG 1 cut(s) 183
SatI GCNGC 5 cut(s) 176, 264, 303, 306, 471
Sau3AI GATC 3 cut(s) 87, 295, 394
Sau96I GGNCC 3 cut(s) 49, 236, 536
ScrFI CCNGG 1 cut(s) 578
SetI ASST 4 cut(s) 43, 233, 356, 582
SfaNI GCATC 1 cut(s) 148
SmiMI CAYNNNNRTG 1 cut(s) 183
Sse9I AATT 2 cut(s) 340, 510
SsiI CCGC 2 cut(s) 239, 435
StyD4I CCNGG 1 cut(s) 576
StyI CCWWGG 1 cut(s) 205
TaaI ACNGT 1 cut(s) 594
TaiI ACGT 1 cut(s) 233
TaqI TCGA 3 cut(s) 156, 189, 461
TaqII GACCGA 1 cut(s) 98
TasI AATT 2 cut(s) 340, 510
TatI WGTACW 1 cut(s) 58
TscAI CASTG 1 cut(s) 558
TseI GCWGC 5 cut(s) 175, 263, 302, 305, 470
TspDTI ATGAA 2 cut(s) 444, 472
TspGWI ACGGA 1 cut(s) 262
TspRI CASTG 1 cut(s) 558
XceI RCATGY 1 cut(s) 59
XmiI GTMKAC 1 cut(s) 582
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.