Rorug01G0233700

Methionine adenosyltransferase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001020
Physical Location & Seq
Forward (+)
33606673 .. 33607758
1086 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0233700.1

Sequence Viewer

Length: 702 bp
ATGGAGGACATGTACGGTCTCCCCTCCTCATCATCATCAGCCCAACCCCATGACTACTTCCCCATCGACGACCTCCTCGATTTATCCAACGACGAAATCTTCTCCAACTCCTCCTCCACTACTTCCTCCACCGACTCCCACCCCAACCACCACCACCACTACACTCCACCTCCTCCTCCTCCCCCAGCTGTCTTCCACCCCCCCTCCACCACCGACTTCACCAACGCCCTCTGCCTCCCGAGCGAGGATGTGGCGGAGCTGGAGTGGCTGTCGCGCTTCGTGGACGACTCCTTCACCGACTTCCCGGGGCCGCCGGAGCTCTCAGGATCCATGTCCCTCCCCAACGACAAGTCGTTCTCCAACCGCGGCCACGCCAAGCCCATTCGCCAAGCGGAAGGCTCCGGGTCCATCCGGAGGTGCACCCACTGCGCCTCGGAGAAGACGCCGCAGTGGCGGACGGGCCCATTGGGCCCGAAGACGCTGTGCAATGCCTGCGGCGTGCGGTACAAGTCGGGTCGGCTCGTGCCCGAGTACCGGCCCGCGGCGAGCCCGACGTTCGTGCTGACTCAGCATTCGAACTCGCACCGGAAGGTGATGGAGCTCCGTCGGCAGAAGGAGGTGTCGTCGCAGCAGGAGTCGGAGGAGGAGGAGGAGCAGCAGAGGCGATTCTATCTTCACCCGAATGGGTTTCGAGTGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RmlD_sub_bind

Protein Analysis

233

Amino Acids

26.11

Weight (kDa)

5.81

Isoelectric Point (pI)

69.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATA PF00320 140 - 174 8.9e-15 GATA zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 274, 366, 542
AccIII TCCGGA 1 cut(s) 411
AclWI GGATC 2 cut(s) 321, 334
AcoI YGGCCR 1 cut(s) 367
AcyI GRCGYC 1 cut(s) 443
AfaI GTAC 3 cut(s) 14, 506, 533
AfiI CCNNNNNNNGG 4 cut(s) 244, 414, 534, 541
AflIII ACRYGT 1 cut(s) 9
AluBI AGCT 4 cut(s) 188, 259, 319, 601
AluI AGCT 4 cut(s) 188, 259, 319, 601
Alw21I GWGCWC 3 cut(s) 321, 422, 603
Alw26I GTCTC 1 cut(s) 23
Alw44I GTGCAC 1 cut(s) 418
AlwI GGATC 2 cut(s) 321, 334
Ama87I CYCGRG 3 cut(s) 238, 304, 527
Aor13HI TCCGGA 1 cut(s) 411
AoxI GGCC 5 cut(s) 308, 367, 460, 469, 536
ApaI GGGCCC 2 cut(s) 464, 473
ApaLI GTGCAC 1 cut(s) 418
ApeKI GCWGC 2 cut(s) 628, 655
ArsI GACNNNNNNTTYG 2 cut(s) 556, 588
AspLEI GCGC 2 cut(s) 276, 431
AspS9I GGNCC 7 cut(s) 308, 405, 460, 461, 469, 470, 537
AsuC2I CCSGG 3 cut(s) 305, 306, 403
AsuHPI GGTGA 4 cut(s) 211, 286, 604, 668
AsuII TTCGAA 1 cut(s) 575
AvaI CYCGRG 3 cut(s) 238, 304, 527
AvaII GGWCC 1 cut(s) 405
BaeGI GKGCMC 4 cut(s) 422, 464, 473, 528
BamHI GGATCC 1 cut(s) 326
BanII GRGCYC 5 cut(s) 321, 464, 473, 551, 603
BauI CACGAG 1 cut(s) 521
BbsI GAAGAC 3 cut(s) 184, 446, 482
Bbv12I GWGCWC 3 cut(s) 321, 422, 603
BbvI GCAGC 2 cut(s) 640, 667
BccI CCATC 3 cut(s) 71, 416, 589
BcgI CGANNNNNNTGC 2 cut(s) 541, 575
BcnI CCSGG 3 cut(s) 305, 306, 403
BcoDI GTCTC 1 cut(s) 23
BglI GCCNNNNNGGC 2 cut(s) 451, 468
BisI GCNGC 7 cut(s) 311, 367, 446, 496, 543, 629, 656
BlsI GCNGC 7 cut(s) 312, 368, 447, 497, 544, 630, 657
Bme1390I CCNGG 3 cut(s) 305, 306, 403
Bme18I GGWCC 1 cut(s) 405
BmeT110I CYCGRG 3 cut(s) 238, 304, 527
BmgT120I GGNCC 7 cut(s) 308, 405, 460, 461, 469, 470, 537
BmiI GGNNCC 6 cut(s) 309, 328, 400, 406, 462, 471
BmrFI CCNGG 3 cut(s) 305, 306, 403
BpiI GAAGAC 3 cut(s) 184, 446, 482
BpmI CTGGAG 1 cut(s) 281
Bpu14I TTCGAA 1 cut(s) 575
BpuMI CCSGG 3 cut(s) 305, 306, 403
BsaHI GRCGYC 1 cut(s) 443
BsaI GGTCTC 1 cut(s) 23
BsaJI CCNNGG 5 cut(s) 304, 305, 364, 432, 540
BsaWI WCCGGW 2 cut(s) 411, 585
Bsc4I CCNNNNNNNGG 4 cut(s) 244, 414, 534, 541
Bse118I RCCGGY 1 cut(s) 534
Bse3DI GCAATG 1 cut(s) 493
BseAI TCCGGA 1 cut(s) 411
BseDI CCNNGG 5 cut(s) 304, 305, 364, 432, 540
BseGI GGATG 2 cut(s) 253, 408
BseLI CCNNNNNNNGG 4 cut(s) 244, 414, 534, 541
BseMI GCAATG 1 cut(s) 493
BseMII CTCAG 2 cut(s) 336, 581
BseSI GKGCMC 4 cut(s) 422, 464, 473, 528
BseXI GCAGC 2 cut(s) 640, 667
BseYI CCCAGC 1 cut(s) 184
Bsh1236I CGCG 3 cut(s) 274, 366, 542
BshFI GGCC 5 cut(s) 310, 369, 462, 471, 538
BsiHKAI GWGCWC 3 cut(s) 321, 422, 603
BsiHKCI CYCGRG 3 cut(s) 238, 304, 527
BsiSI CCGG 6 cut(s) 305, 314, 402, 412, 535, 586
BslFI GGGAC 1 cut(s) 319
BslI CCNNNNNNNGG 4 cut(s) 244, 414, 534, 541
BsmAI GTCTC 1 cut(s) 23
BsmFI GGGAC 1 cut(s) 319
BsmI GAATGC 1 cut(s) 571
BsnI GGCC 5 cut(s) 310, 369, 462, 471, 538
Bso31I GGTCTC 1 cut(s) 23
BsoBI CYCGRG 3 cut(s) 238, 304, 527
Bsp119I TTCGAA 1 cut(s) 575
Bsp120I GGGCCC 2 cut(s) 460, 469
Bsp1286I GDGCHC 7 cut(s) 321, 422, 464, 473, 528, 551, 603
Bsp13I TCCGGA 1 cut(s) 411
Bsp143I GATC 1 cut(s) 326
BspANI GGCC 5 cut(s) 310, 369, 462, 471, 538
BspCNI CTCAG 2 cut(s) 335, 580
BspEI TCCGGA 1 cut(s) 411
BspFNI CGCG 3 cut(s) 274, 366, 542
BspLI GGNNCC 6 cut(s) 309, 328, 400, 406, 462, 471
BspPI GGATC 2 cut(s) 321, 334
BspT104I TTCGAA 1 cut(s) 575
BspTNI GGTCTC 1 cut(s) 23
BsrDI GCAATG 1 cut(s) 493
BsrFI RCCGGY 1 cut(s) 534
BssAI RCCGGY 1 cut(s) 534
BssECI CCNNGG 5 cut(s) 304, 305, 364, 432, 540
BssMI GATC 1 cut(s) 326
BssNI GRCGYC 1 cut(s) 443
BssSI CACGAG 1 cut(s) 521
Bst2BI CACGAG 1 cut(s) 521
Bst4CI ACNGT 1 cut(s) 17
BstACI GRCGYC 1 cut(s) 443
BstAPI GCANNNNNTGC 2 cut(s) 426, 492
BstBI TTCGAA 1 cut(s) 575
BstC8I GCNNGC 4 cut(s) 493, 500, 540, 547
BstDEI CTNAG 2 cut(s) 322, 567
BstDSI CCRYGG 2 cut(s) 364, 540
BstF5I GGATG 2 cut(s) 253, 408
BstFNI CGCG 3 cut(s) 274, 366, 542
BstHHI GCGC 2 cut(s) 276, 431
BstKTI GATC 1 cut(s) 329
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 1 cut(s) 326
BstNSI RCATGY 1 cut(s) 13
BstSCI CCNGG 3 cut(s) 303, 304, 401
BstSLI GKGCMC 4 cut(s) 422, 464, 473, 528
BstUI CGCG 3 cut(s) 274, 366, 542
BstV1I GCAGC 2 cut(s) 640, 667
BstV2I GAAGAC 3 cut(s) 184, 446, 482
BstX2I RGATCY 1 cut(s) 326
BstYI RGATCY 1 cut(s) 326
BsuRI GGCC 5 cut(s) 310, 369, 462, 471, 538
BtgI CCRYGG 2 cut(s) 364, 540
BtsCI GGATG 2 cut(s) 253, 408
BtsI GCAGTG 2 cut(s) 424, 455
BtsIMutI CAGTG 2 cut(s) 424, 455
Cac8I GCNNGC 4 cut(s) 493, 500, 540, 547
CfoI GCGC 2 cut(s) 276, 431
Cfr10I RCCGGY 1 cut(s) 534
Cfr13I GGNCC 7 cut(s) 308, 405, 460, 461, 469, 470, 537
Cfr42I CCGCGG 2 cut(s) 367, 543
Cfr9I CCCGGG 1 cut(s) 304
CseI GACGC 2 cut(s) 451, 487
Csp6I GTAC 3 cut(s) 13, 505, 532
CviAII CATG 3 cut(s) 10, 50, 331
CviQI GTAC 3 cut(s) 13, 505, 532
DdeI CTNAG 2 cut(s) 322, 567
DpnI GATC 1 cut(s) 328
DpnII GATC 1 cut(s) 326
EaeI YGGCCR 1 cut(s) 367
EciI GGCGGA 2 cut(s) 269, 469
Ecl136II GAGCTC 2 cut(s) 319, 601
Eco24I GRGCYC 5 cut(s) 321, 464, 473, 551, 603
Eco31I GGTCTC 1 cut(s) 23
Eco47I GGWCC 1 cut(s) 405
Eco53kI GAGCTC 2 cut(s) 319, 601
Eco88I CYCGRG 3 cut(s) 238, 304, 527
EcoICRI GAGCTC 2 cut(s) 319, 601
EcoT38I GRGCYC 5 cut(s) 321, 464, 473, 551, 603
FaeI CATG 3 cut(s) 13, 53, 334
FaiI YATR 3 cut(s) 11, 51, 332
FaqI GGGAC 1 cut(s) 319
FatI CATG 3 cut(s) 9, 49, 330
FauI CCCGC 1 cut(s) 547
Fnu4HI GCNGC 7 cut(s) 311, 367, 446, 496, 543, 629, 656
FokI GGATG 2 cut(s) 260, 395
FriOI GRGCYC 5 cut(s) 321, 464, 473, 551, 603
Fsp4HI GCNGC 7 cut(s) 311, 367, 446, 496, 543, 629, 656
GlaI GCGC 2 cut(s) 275, 430
GluI GCNGC 7 cut(s) 311, 367, 446, 496, 543, 629, 656
GsaI CCCAGC 1 cut(s) 188
GsuI CTGGAG 1 cut(s) 281
HaeIII GGCC 5 cut(s) 310, 369, 462, 471, 538
HapII CCGG 6 cut(s) 305, 314, 402, 412, 535, 586
HgaI GACGC 2 cut(s) 451, 487
HhaI GCGC 2 cut(s) 276, 431
Hin1I GRCGYC 1 cut(s) 443
Hin1II CATG 3 cut(s) 13, 53, 334
Hin6I GCGC 2 cut(s) 274, 429
HinP1I GCGC 2 cut(s) 274, 429
HinfI GANTC 5 cut(s) 134, 287, 565, 635, 666
HpaII CCGG 6 cut(s) 305, 314, 402, 412, 535, 586
HphI GGTGA 4 cut(s) 211, 286, 604, 668
Hpy166II GTNNAC 2 cut(s) 283, 420
Hpy188I TCNGA 2 cut(s) 436, 640
Hpy188III TCNNGA 3 cut(s) 238, 324, 412
Hpy8I GTNNAC 2 cut(s) 283, 420
Hpy99I CGWCG 5 cut(s) 71, 95, 556, 609, 628
HpyAV CCTTC 4 cut(s) 301, 389, 583, 607
HpyCH4III ACNGT 1 cut(s) 17
HpyCH4IV ACGT 1 cut(s) 554
HpyCH4V TGCA 2 cut(s) 420, 486
HpyF3I CTNAG 2 cut(s) 322, 567
HpySE526I ACGT 1 cut(s) 554
Hsp92I GRCGYC 1 cut(s) 443
Hsp92II CATG 3 cut(s) 13, 53, 334
HspAI GCGC 2 cut(s) 274, 429
Kpn2I TCCGGA 1 cut(s) 411
KspI CCGCGG 2 cut(s) 367, 543
Kzo9I GATC 1 cut(s) 326
LmnI GCTCC 6 cut(s) 256, 316, 404, 598, 606, 652
Lsp1109I GCAGC 2 cut(s) 640, 667
MaeII ACGT 1 cut(s) 554
MalI GATC 1 cut(s) 328
MboI GATC 1 cut(s) 326
MboII GAAGA 5 cut(s) 91, 184, 451, 487, 665
MflI RGATCY 1 cut(s) 326
MhlI GDGCHC 7 cut(s) 321, 422, 464, 473, 528, 551, 603
MlyI GAGTC 4 cut(s) 128, 281, 559, 644
MmeI TCCRAC 4 cut(s) 111, 129, 384, 618
MroI TCCGGA 1 cut(s) 411
MslI CAYNNNNRTG 1 cut(s) 681
MspA1I CMGCKG 3 cut(s) 188, 366, 542
MspI CCGG 6 cut(s) 305, 314, 402, 412, 535, 586
MspR9I CCNGG 3 cut(s) 305, 306, 403
Mva1269I GAATGC 1 cut(s) 571
MvnI CGCG 3 cut(s) 274, 366, 542
NciI CCSGG 3 cut(s) 305, 306, 403
NdeII GATC 1 cut(s) 326
NlaIII CATG 3 cut(s) 13, 53, 334
NlaIV GGNNCC 6 cut(s) 309, 328, 400, 406, 462, 471
NspI RCATGY 1 cut(s) 13
NspV TTCGAA 1 cut(s) 575
PciI ACATGT 1 cut(s) 9
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 571
PfeI GAWTC 1 cut(s) 666
PkrI GCNGC 7 cut(s) 312, 368, 447, 497, 544, 630, 657
PleI GAGTC 4 cut(s) 128, 281, 559, 643
PpsI GAGTC 4 cut(s) 128, 281, 559, 643
PscI ACATGT 1 cut(s) 9
Psp124BI GAGCTC 2 cut(s) 321, 603
PspFI CCCAGC 1 cut(s) 184
PspN4I GGNNCC 6 cut(s) 309, 328, 400, 406, 462, 471
PspOMI GGGCCC 2 cut(s) 460, 469
PspPI GGNCC 7 cut(s) 308, 405, 460, 461, 469, 470, 537
PsuI RGATCY 1 cut(s) 326
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 3 cut(s) 14, 506, 533
RsaNI GTAC 3 cut(s) 13, 505, 532
RseI CAYNNNNRTG 1 cut(s) 681
SacI GAGCTC 2 cut(s) 321, 603
SacII CCGCGG 2 cut(s) 367, 543
SatI GCNGC 7 cut(s) 311, 367, 446, 496, 543, 629, 656
Sau3AI GATC 1 cut(s) 326
Sau96I GGNCC 7 cut(s) 308, 405, 460, 461, 469, 470, 537
SchI GAGTC 4 cut(s) 128, 281, 559, 644
ScrFI CCNGG 3 cut(s) 305, 306, 403
SduI GDGCHC 7 cut(s) 321, 422, 464, 473, 528, 551, 603
SfiI GGCCNNNNNGGCC 1 cut(s) 468
Sfr303I CCGCGG 2 cut(s) 367, 543
SfuI TTCGAA 1 cut(s) 575
SgrBI CCGCGG 2 cut(s) 367, 543
SinI GGWCC 1 cut(s) 405
SmaI CCCGGG 1 cut(s) 306
SmiMI CAYNNNNRTG 1 cut(s) 681
SstI GAGCTC 2 cut(s) 321, 603
StyD4I CCNGG 3 cut(s) 303, 304, 401
TaaI ACNGT 1 cut(s) 17
TaiI ACGT 1 cut(s) 557
TaqI TCGA 4 cut(s) 66, 78, 575, 691
TauI GCSGC 5 cut(s) 313, 369, 448, 498, 545
TfiI GAWTC 1 cut(s) 666
TscAI CASTG 2 cut(s) 431, 455
TseI GCWGC 2 cut(s) 628, 655
TspGWI ACGGA 1 cut(s) 593
TspMI CCCGGG 1 cut(s) 304
TspRI CASTG 2 cut(s) 431, 455
VneI GTGCAC 1 cut(s) 418
VpaK11BI GGWCC 1 cut(s) 405
XceI RCATGY 1 cut(s) 13
XmaI CCCGGG 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.