Rroxscaffold_4G00301980

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001020
Physical Location & Seq
Reverse (-)
22342624 .. 22345981
3358 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00301980.1

Sequence Viewer

Length: 441 bp
ATGGGAGGAGGCGAGCATGCGCACGGCGAAGGGGCCCACGGCGGAGACTTCAGGGCGAAGGTGTGGAGCATGAGCGGTGGGCCCTACTGCAGGCCCAAGCACTGGAAGCGCAACACGGCCTTCGCCATGTTCGGAGTCTTCCTCATCTGTATTCCCATCGCCATGAAATCTGCCGAGCTCGAGATTAAATCTTTTGCTGTCTTGAATGCTTTGAATATCTACGACAAGCTACTAGTGGTATCCCGTGTTCAAATGGCTGAGACGGTTGCTGATATAAGGGCATACAATCTCTCATTAATTAAATCTGTATCCGCATCATCGGTTGATCGTGGAGTTATGTCTCCTGCTGACATATCCATGGATAGAACTAAGCTAGTTCAGATGCTTGGTATTTCTCCTATTTCATTTATAGATGGTGTCAGATTGACGCTTGAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

146

Amino Acids

15.89

Weight (kDa)

9.0

Isoelectric Point (pI)

26.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 21
AccB7I CCANNNNNTGG 1 cut(s) 102
AccBSI CCGCTC 1 cut(s) 75
AciI CCGC 3 cut(s) 42, 75, 312
AcuI CTGAAG 1 cut(s) 34
AfiI CCNNNNNNNGG 2 cut(s) 90, 102
AgsI TTSAA 4 cut(s) 205, 214, 251, 434
AhlI ACTAGT 1 cut(s) 232
AluBI AGCT 3 cut(s) 178, 229, 373
AluI AGCT 3 cut(s) 178, 229, 373
Alw21I GWGCWC 1 cut(s) 180
Alw26I GTCTC 3 cut(s) 39, 254, 345
Ama87I CYCGRG 1 cut(s) 179
AoxI GGCC 4 cut(s) 33, 80, 92, 117
ApaI GGGCCC 2 cut(s) 37, 84
AseI ATTAAT 1 cut(s) 296
AspLEI GCGC 2 cut(s) 22, 111
AspS9I GGNCC 5 cut(s) 33, 34, 80, 81, 93
AvaI CYCGRG 1 cut(s) 179
BaeGI GKGCMC 2 cut(s) 37, 84
BanII GRGCYC 3 cut(s) 37, 84, 180
BbsI GAAGAC 1 cut(s) 130
Bbv12I GWGCWC 1 cut(s) 180
BccI CCATC 2 cut(s) 164, 407
BceAI ACGGC 3 cut(s) 40, 55, 132
BciVI GTATCC 2 cut(s) 250, 319
BcoDI GTCTC 3 cut(s) 39, 254, 345
BcuI ACTAGT 1 cut(s) 232
BfaI CTAG 2 cut(s) 233, 374
BfmI CTRYAG 1 cut(s) 88
BfuI GTATCC 2 cut(s) 250, 319
BmeT110I CYCGRG 1 cut(s) 179
BmgT120I GGNCC 5 cut(s) 33, 34, 80, 81, 93
BmiI GGNNCC 3 cut(s) 34, 35, 82
BmsI GCATC 2 cut(s) 323, 372
BpiI GAAGAC 1 cut(s) 130
BplI GAGNNNNNCTC 2 cut(s) 126, 158
BsaJI CCNNGG 2 cut(s) 37, 357
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 102
Bse1I ACTGG 1 cut(s) 107
BseDI CCNNGG 2 cut(s) 37, 357
BseLI CCNNNNNNNGG 2 cut(s) 90, 102
BseMII CTCAG 1 cut(s) 249
BseNI ACTGG 1 cut(s) 107
BseRI GAGGAG 1 cut(s) 21
BseSI GKGCMC 2 cut(s) 37, 84
BshFI GGCC 4 cut(s) 35, 82, 94, 119
BsiHKAI GWGCWC 1 cut(s) 180
BsiHKCI CYCGRG 1 cut(s) 179
BslI CCNNNNNNNGG 2 cut(s) 90, 102
BsmAI GTCTC 3 cut(s) 39, 254, 345
BsmBI CGTCTC 1 cut(s) 254
BsmI GAATGC 1 cut(s) 211
BsnI GGCC 4 cut(s) 35, 82, 94, 119
BsoBI CYCGRG 1 cut(s) 179
Bsp120I GGGCCC 2 cut(s) 33, 80
Bsp1286I GDGCHC 3 cut(s) 37, 84, 180
Bsp143I GATC 1 cut(s) 325
Bsp19I CCATGG 1 cut(s) 357
BspACI CCGC 3 cut(s) 42, 75, 312
BspANI GGCC 4 cut(s) 35, 82, 94, 119
BspCNI CTCAG 1 cut(s) 250
BspLI GGNNCC 3 cut(s) 34, 35, 82
BspMAI CTGCAG 1 cut(s) 92
BsrBI CCGCTC 1 cut(s) 75
BsrI ACTGG 1 cut(s) 107
BssECI CCNNGG 2 cut(s) 37, 357
BssMI GATC 1 cut(s) 325
BssT1I CCWWGG 1 cut(s) 357
Bst4CI ACNGT 2 cut(s) 265, 438
BstC8I GCNNGC 3 cut(s) 14, 18, 92
BstDEI CTNAG 2 cut(s) 258, 369
BstDSI CCRYGG 2 cut(s) 37, 357
BstENI CCTNNNNNAGG 1 cut(s) 88
BstHHI GCGC 2 cut(s) 22, 111
BstKTI GATC 1 cut(s) 328
BstMAI GTCTC 3 cut(s) 39, 254, 345
BstMBI GATC 1 cut(s) 325
BstMWI GCNNNNNNNGC 1 cut(s) 106
BstNSI RCATGY 1 cut(s) 20
BstSFI CTRYAG 1 cut(s) 88
BstSLI GKGCMC 2 cut(s) 37, 84
BstV2I GAAGAC 1 cut(s) 130
BsuI GTATCC 2 cut(s) 250, 319
BsuRI GGCC 4 cut(s) 35, 82, 94, 119
BtgI CCRYGG 2 cut(s) 37, 357
BtgZI GCGATG 1 cut(s) 142
BtsIMutI CAGTG 1 cut(s) 100
Cac8I GCNNGC 3 cut(s) 14, 18, 92
CfoI GCGC 2 cut(s) 22, 111
Cfr13I GGNCC 5 cut(s) 33, 34, 80, 81, 93
CseI GACGC 1 cut(s) 436
CviAII CATG 5 cut(s) 17, 70, 127, 163, 358
CviJI RGCY 8 cut(s) 35, 82, 94, 119, 178, 229, 257, 373
CviKI_1 RGCY 8 cut(s) 35, 82, 94, 119, 178, 229, 257, 373
DdeI CTNAG 2 cut(s) 258, 369
DpnI GATC 1 cut(s) 327
DpnII GATC 1 cut(s) 325
EciI GGCGGA 1 cut(s) 57
Ecl136II GAGCTC 1 cut(s) 178
Eco130I CCWWGG 1 cut(s) 357
Eco24I GRGCYC 3 cut(s) 37, 84, 180
Eco53kI GAGCTC 1 cut(s) 178
Eco57I CTGAAG 1 cut(s) 34
Eco88I CYCGRG 1 cut(s) 179
EcoICRI GAGCTC 1 cut(s) 178
EcoNI CCTNNNNNAGG 1 cut(s) 88
EcoO109I RGGNCCY 2 cut(s) 33, 81
EcoT14I CCWWGG 1 cut(s) 357
EcoT38I GRGCYC 3 cut(s) 37, 84, 180
ErhI CCWWGG 1 cut(s) 357
Esp3I CGTCTC 1 cut(s) 254
FaeI CATG 5 cut(s) 20, 73, 130, 166, 361
FatI CATG 5 cut(s) 16, 69, 126, 162, 357
FriOI GRGCYC 3 cut(s) 37, 84, 180
FspAI RTGCGCAY 1 cut(s) 21
FspBI CTAG 2 cut(s) 233, 374
FspI TGCGCA 1 cut(s) 21
GlaI GCGC 2 cut(s) 21, 110
HaeIII GGCC 4 cut(s) 35, 82, 94, 119
HgaI GACGC 1 cut(s) 436
HhaI GCGC 2 cut(s) 22, 111
Hin1II CATG 5 cut(s) 20, 73, 130, 166, 361
Hin6I GCGC 2 cut(s) 20, 109
HinP1I GCGC 2 cut(s) 20, 109
HinfI GANTC 1 cut(s) 135
Hpy188I TCNGA 3 cut(s) 134, 381, 422
Hpy188III TCNNGA 2 cut(s) 181, 202
HpyAV CCTTC 3 cut(s) 23, 52, 130
HpyCH4III ACNGT 2 cut(s) 265, 438
HpyCH4V TGCA 1 cut(s) 90
HpyF10VI GCNNNNNNNGC 1 cut(s) 106
HpyF3I CTNAG 2 cut(s) 258, 369
Hsp92II CATG 5 cut(s) 20, 73, 130, 166, 361
HspAI GCGC 2 cut(s) 20, 109
Kzo9I GATC 1 cut(s) 325
LmnI GCTCC 1 cut(s) 66
LpnPI CCDG 4 cut(s) 37, 76, 88, 357
LweI GCATC 2 cut(s) 323, 372
MaeI CTAG 2 cut(s) 233, 374
MalI GATC 1 cut(s) 327
MbiI CCGCTC 1 cut(s) 75
MboI GATC 1 cut(s) 325
MboII GAAGA 1 cut(s) 130
MhlI GDGCHC 3 cut(s) 37, 84, 180
MluCI AATT 1 cut(s) 297
MlyI GAGTC 1 cut(s) 144
MnlI CCTC 1 cut(s) 152
MseI TTAA 3 cut(s) 186, 296, 300
MslI CAYNNNNRTG 2 cut(s) 161, 356
Mva1269I GAATGC 1 cut(s) 211
MwoI GCNNNNNNNGC 1 cut(s) 106
NcoI CCATGG 1 cut(s) 357
NdeII GATC 1 cut(s) 325
NlaIII CATG 5 cut(s) 20, 73, 130, 166, 361
NlaIV GGNNCC 3 cut(s) 34, 35, 82
NmeAIII GCCGAG 1 cut(s) 199
NsbI TGCGCA 1 cut(s) 21
NspI RCATGY 1 cut(s) 20
PacI TTAATTAA 1 cut(s) 300
PaeI GCATGC 1 cut(s) 20
PaeR7I CTCGAG 1 cut(s) 179
PctI GAATGC 1 cut(s) 211
PflMI CCANNNNNTGG 1 cut(s) 102
PleI GAGTC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 143
PshBI ATTAAT 1 cut(s) 296
Psp124BI GAGCTC 1 cut(s) 180
PspN4I GGNNCC 3 cut(s) 34, 35, 82
PspOMI GGGCCC 2 cut(s) 33, 80
PspPI GGNCC 5 cut(s) 33, 34, 80, 81, 93
PsrI GAACNNNNNNTAC 2 cut(s) 231, 263
PstI CTGCAG 1 cut(s) 92
RseI CAYNNNNRTG 2 cut(s) 161, 356
SacI GAGCTC 1 cut(s) 180
SaqAI TTAA 3 cut(s) 186, 296, 300
Sau3AI GATC 1 cut(s) 325
Sau96I GGNCC 5 cut(s) 33, 34, 80, 81, 93
SchI GAGTC 1 cut(s) 144
SduI GDGCHC 3 cut(s) 37, 84, 180
SetI ASST 4 cut(s) 63, 180, 231, 375
SfaNI GCATC 2 cut(s) 323, 372
SfcI CTRYAG 1 cut(s) 88
Sfr274I CTCGAG 1 cut(s) 179
SlaI CTCGAG 1 cut(s) 179
SmiMI CAYNNNNRTG 2 cut(s) 161, 356
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
SpeI ACTAGT 1 cut(s) 232
SphI GCATGC 1 cut(s) 20
Sse9I AATT 1 cut(s) 297
SsiI CCGC 3 cut(s) 42, 75, 312
SspMI CTAG 2 cut(s) 233, 374
SstI GAGCTC 1 cut(s) 180
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 2 cut(s) 265, 438
TaqI TCGA 1 cut(s) 180
TasI AATT 1 cut(s) 297
Tru1I TTAA 3 cut(s) 186, 296, 300
Tru9I TTAA 3 cut(s) 186, 296, 300
TscAI CASTG 1 cut(s) 107
TspDTI ATGAA 2 cut(s) 179, 393
TspRI CASTG 1 cut(s) 107
Van91I CCANNNNNTGG 1 cut(s) 102
VspI ATTAAT 1 cut(s) 296
XagI CCTNNNNNAGG 1 cut(s) 88
XceI RCATGY 1 cut(s) 20
XhoI CTCGAG 1 cut(s) 179
XspI CTAG 2 cut(s) 233, 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.