RchiOBHm_Chr1g0353241

Methionine adenosyltransferase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001020
Physical Location & Seq
Forward (+)
46506186 .. 46508741
2556 bp
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UTR
Exon/CDS
Intron
PRQ57889

Sequence Viewer

Length: 759 bp
ATGGATTTGAAGCCATTTCTCATCAGTTTGGTCCGATCAATTTTGTTTACTGGGTTTGAGTTTTTTGAATGCCATACTGCCCTGTTCTGCTGTTGGCCTGATGTGGTAGTAAACTGTGCTGCACTATCGGTTCATCGTGCCTGTGAAATGGATCCTGCAGCAGCTATGTCAGTTAATGTGCCATCTTCTCTTGTTAATTGGTTATCAAGCTTAGAAGAGAGTAATTATCTACTGATCCATTTGTCAACTGATCAAGTTTATGAAGGGGTGAAGTCTTTTTACAAGGAAGATGATGAAGTTGTACCAGTAAATGTTTATGGGAAATCAAAAGTGGCAGCTGAGCAGTTCATTACTGAGAAATGCTCGAACTTTGCAATTTTGAGAAGCAGTATCATCTTTGGGCCACAGACAATCTCACCGGTTTCAAAATCTCTTCCGATTCAGTGGGTTGATGGTGTCCTCTCCAAAGGAAATACAACCGAATTCTTTCATGATGAGTTTCGATGCCCTGTGTATGTAAAGGATGTCATAGCAATCATACTTGCTTTGTCCAAGACATGGATATCAGAGGCTAAGCAAAGAAATTTGCTACTGAATGTTGGTGGACTGGACAGGGTATCCCGTGTTCAAATGGCTGAGACAGTTGCTGATATAAGGGGATACAACCTCTCATTAATTAAATCTGTATCTGCATCATCGACGCTTGGTATTTCTCCTATTTCATTTCGAGATGGTGTCAGATTGACGCTTGAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RmlD_sub_bind

Protein Analysis

252

Amino Acids

27.95

Weight (kDa)

5.43

Isoelectric Point (pI)

39.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 32 - 232 1.2e-30 RmlD substrate binding domain
GDP_Man_Dehyd PF16363 32 - 123 4.6e-06 GDP-mannose 4,6 dehydratase
Epimerase PF01370 33 - 186 1.1e-12 NAD dependent epimerase/dehydratase family
NAD_binding_4 PF07993 34 - 165 7.5e-06 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 558
AclWI GGATC 3 cut(s) 146, 159, 229
AcsI RAATTY 2 cut(s) 482, 583
AfaI GTAC 1 cut(s) 303
AfiI CCNNNNNNNGG 1 cut(s) 558
AgeI ACCGGT 1 cut(s) 418
AgsI TTSAA 5 cut(s) 10, 68, 426, 629, 752
AluBI AGCT 3 cut(s) 164, 210, 338
AluI AGCT 3 cut(s) 164, 210, 338
Alw26I GTCTC 1 cut(s) 632
AlwI GGATC 3 cut(s) 146, 159, 229
AlwNI CAGNNNCTG 1 cut(s) 647
AoxI GGCC 2 cut(s) 95, 401
ApeKI GCWGC 4 cut(s) 119, 158, 161, 335
ApoI RAATTY 2 cut(s) 482, 583
AseI ATTAAT 1 cut(s) 674
AsiGI ACCGGT 1 cut(s) 418
Asp700I GAANNNNTTC 1 cut(s) 486
AspS9I GGNCC 2 cut(s) 31, 401
AsuHPI GGTGA 2 cut(s) 280, 408
AvaII GGWCC 1 cut(s) 31
BamHI GGATCC 1 cut(s) 151
BarI GAAGNNNNNNTAC 2 cut(s) 263, 295
BbvI GCAGC 4 cut(s) 106, 170, 173, 347
BccI CCATC 3 cut(s) 190, 446, 725
BciVI GTATCC 2 cut(s) 628, 653
BclI TGATCA 1 cut(s) 250
BcoDI GTCTC 1 cut(s) 632
BfmI CTRYAG 1 cut(s) 156
BfuI GTATCC 2 cut(s) 628, 653
BisI GCNGC 4 cut(s) 120, 159, 162, 336
BlpI GCTNAGC 2 cut(s) 339, 573
BlsI GCNGC 4 cut(s) 121, 160, 163, 337
Bme18I GGWCC 1 cut(s) 31
BmgT120I GGNCC 2 cut(s) 31, 401
BmiI GGNNCC 1 cut(s) 153
BmrI ACTGGG 1 cut(s) 60
BmsI GCATC 2 cut(s) 494, 701
BmuI ACTGGG 1 cut(s) 60
BplI GAGNNNNNCTC 2 cut(s) 347, 379
Bpu1102I GCTNAGC 2 cut(s) 339, 573
BsaWI WCCGGW 1 cut(s) 418
Bsc4I CCNNNNNNNGG 1 cut(s) 558
Bse118I RCCGGY 1 cut(s) 418
Bse1I ACTGG 3 cut(s) 55, 305, 612
BseGI GGATG 1 cut(s) 529
BseLI CCNNNNNNNGG 1 cut(s) 558
BseMII CTCAG 3 cut(s) 330, 345, 627
BseNI ACTGG 3 cut(s) 55, 305, 612
BseXI GCAGC 4 cut(s) 106, 170, 173, 347
BsgI GTGCAG 1 cut(s) 105
BshFI GGCC 2 cut(s) 97, 403
BshTI ACCGGT 1 cut(s) 418
BsiSI CCGG 1 cut(s) 419
BslI CCNNNNNNNGG 1 cut(s) 558
BsmAI GTCTC 1 cut(s) 632
BsmI GAATGC 1 cut(s) 74
BsnI GGCC 2 cut(s) 97, 403
Bsp143I GATC 4 cut(s) 35, 151, 234, 250
Bsp1720I GCTNAGC 2 cut(s) 339, 573
BspANI GGCC 2 cut(s) 97, 403
BspCNI CTCAG 3 cut(s) 331, 346, 628
BspHI TCATGA 1 cut(s) 490
BspLI GGNNCC 1 cut(s) 153
BspMAI CTGCAG 1 cut(s) 160
BspPI GGATC 3 cut(s) 146, 159, 229
BsrFI RCCGGY 1 cut(s) 418
BsrI ACTGG 3 cut(s) 55, 305, 612
BssAI RCCGGY 1 cut(s) 418
BssMI GATC 4 cut(s) 35, 151, 234, 250
Bst4CI ACNGT 3 cut(s) 116, 643, 756
Bst6I CTCTTC 2 cut(s) 210, 438
BstDEI CTNAG 5 cut(s) 211, 339, 354, 573, 636
BstF5I GGATG 1 cut(s) 529
BstKTI GATC 4 cut(s) 38, 154, 237, 253
BstMAI GTCTC 1 cut(s) 632
BstMBI GATC 4 cut(s) 35, 151, 234, 250
BstSFI CTRYAG 1 cut(s) 156
BstV1I GCAGC 4 cut(s) 106, 170, 173, 347
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BsuI GTATCC 2 cut(s) 628, 653
BsuRI GGCC 2 cut(s) 97, 403
BtsCI GGATG 1 cut(s) 529
BtsIMutI CAGTG 1 cut(s) 449
CaiI CAGNNNCTG 1 cut(s) 647
CciI TCATGA 1 cut(s) 490
Cfr10I RCCGGY 1 cut(s) 418
Cfr13I GGNCC 2 cut(s) 31, 401
CseI GACGC 2 cut(s) 709, 754
Csp6I GTAC 1 cut(s) 302
CspAI ACCGGT 1 cut(s) 418
CviAII CATG 2 cut(s) 491, 558
CviJI RGCY 8 cut(s) 13, 97, 164, 210, 338, 403, 572, 635
CviKI_1 RGCY 8 cut(s) 13, 97, 164, 210, 338, 403, 572, 635
CviQI GTAC 1 cut(s) 302
DdeI CTNAG 5 cut(s) 211, 339, 354, 573, 636
DpnI GATC 4 cut(s) 37, 153, 236, 252
DpnII GATC 4 cut(s) 35, 151, 234, 250
Eam1104I CTCTTC 2 cut(s) 210, 438
EarI CTCTTC 2 cut(s) 210, 438
Eco32I GATATC 1 cut(s) 564
Eco47I GGWCC 1 cut(s) 31
EcoRI GAATTC 1 cut(s) 482
EcoRV GATATC 1 cut(s) 564
FaeI CATG 2 cut(s) 494, 561
FatI CATG 2 cut(s) 490, 557
FbaI TGATCA 1 cut(s) 250
Fnu4HI GCNGC 4 cut(s) 120, 159, 162, 336
FokI GGATG 1 cut(s) 536
Fsp4HI GCNGC 4 cut(s) 120, 159, 162, 336
GluI GCNGC 4 cut(s) 120, 159, 162, 336
HaeIII GGCC 2 cut(s) 97, 403
HapII CCGG 1 cut(s) 419
HgaI GACGC 2 cut(s) 709, 754
Hin1II CATG 2 cut(s) 494, 561
HincII GTYRAC 1 cut(s) 246
HindII GTYRAC 1 cut(s) 246
HindIII AAGCTT 1 cut(s) 208
HinfI GANTC 1 cut(s) 439
HpaII CCGG 1 cut(s) 419
HphI GGTGA 2 cut(s) 280, 408
Hpy166II GTNNAC 4 cut(s) 48, 112, 246, 605
Hpy188I TCNGA 4 cut(s) 35, 438, 568, 740
Hpy188III TCNNGA 2 cut(s) 491, 728
Hpy8I GTNNAC 4 cut(s) 48, 112, 246, 605
Hpy99I CGWCG 1 cut(s) 703
HpyAV CCTTC 1 cut(s) 257
HpyCH4III ACNGT 3 cut(s) 116, 643, 756
HpyCH4V TGCA 4 cut(s) 122, 158, 374, 692
HpyF3I CTNAG 5 cut(s) 211, 339, 354, 573, 636
Hsp92II CATG 2 cut(s) 494, 561
Ksp22I TGATCA 1 cut(s) 250
Kzo9I GATC 4 cut(s) 35, 151, 234, 250
Lsp1109I GCAGC 4 cut(s) 106, 170, 173, 347
LweI GCATC 2 cut(s) 494, 701
MalI GATC 4 cut(s) 37, 153, 236, 252
MboI GATC 4 cut(s) 35, 151, 234, 250
MboII GAAGA 4 cut(s) 177, 227, 299, 425
MflI RGATCY 1 cut(s) 151
MluCI AATT 7 cut(s) 39, 196, 223, 375, 482, 583, 675
MnlI CCTC 3 cut(s) 470, 562, 677
MroXI GAANNNNTTC 1 cut(s) 486
MseI TTAA 4 cut(s) 174, 195, 674, 678
MspA1I CMGCKG 1 cut(s) 338
MspI CCGG 1 cut(s) 419
Mva1269I GAATGC 1 cut(s) 74
NdeII GATC 4 cut(s) 35, 151, 234, 250
NlaIII CATG 2 cut(s) 494, 561
NlaIV GGNNCC 1 cut(s) 153
PacI TTAATTAA 1 cut(s) 678
PagI TCATGA 1 cut(s) 490
PctI GAATGC 1 cut(s) 74
PdmI GAANNNNTTC 1 cut(s) 486
PfeI GAWTC 1 cut(s) 439
PflMI CCANNNNNTGG 1 cut(s) 558
PinAI ACCGGT 1 cut(s) 418
PkrI GCNGC 4 cut(s) 121, 160, 163, 337
PshBI ATTAAT 1 cut(s) 674
PspN4I GGNNCC 1 cut(s) 153
PspPI GGNCC 2 cut(s) 31, 401
PsrI GAACNNNNNNTAC 2 cut(s) 609, 641
PstI CTGCAG 1 cut(s) 160
PstNI CAGNNNCTG 1 cut(s) 647
PsuI RGATCY 1 cut(s) 151
PvuII CAGCTG 1 cut(s) 338
RsaI GTAC 1 cut(s) 303
RsaNI GTAC 1 cut(s) 302
SaqAI TTAA 4 cut(s) 174, 195, 674, 678
SatI GCNGC 4 cut(s) 120, 159, 162, 336
Sau3AI GATC 4 cut(s) 35, 151, 234, 250
Sau96I GGNCC 2 cut(s) 31, 401
SetI ASST 4 cut(s) 166, 212, 340, 669
SfaNI GCATC 2 cut(s) 494, 701
SfcI CTRYAG 1 cut(s) 156
SinI GGWCC 1 cut(s) 31
Sse9I AATT 7 cut(s) 39, 196, 223, 375, 482, 583, 675
TaaI ACNGT 3 cut(s) 116, 643, 756
TaqI TCGA 4 cut(s) 365, 502, 698, 727
TasI AATT 7 cut(s) 39, 196, 223, 375, 482, 583, 675
TfiI GAWTC 1 cut(s) 439
Tru1I TTAA 4 cut(s) 174, 195, 674, 678
Tru9I TTAA 4 cut(s) 174, 195, 674, 678
TscAI CASTG 1 cut(s) 449
TseI GCWGC 4 cut(s) 119, 158, 161, 335
TspDTI ATGAA 6 cut(s) 122, 276, 309, 337, 479, 711
TspRI CASTG 1 cut(s) 449
Van91I CCANNNNNTGG 1 cut(s) 558
VpaK11BI GGWCC 1 cut(s) 31
VspI ATTAAT 1 cut(s) 674
XapI RAATTY 2 cut(s) 482, 583
XmnI GAANNNNTTC 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.