RchiOBHm_Chr1g0353141

Methionine adenosyltransferase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001020
Physical Location & Seq
Forward (+)
46466567 .. 46469306
2740 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57879

Sequence Viewer

Length: 771 bp
ATGGAATTGAAGTCATTTCTCATCTGTTTGGTCCGGTCACCTGATGTGGTAGTAAACTGTGCTGCACTTTCGGTTCCTCGTGCCTGTGAAATGGATCCTGCTGCAGCTATGTCAGTTAATGTGCCATCTTCTCTTGTTAATTGGTTATCGAGCTTAGAAGAGACTCAAGAGAGTAATTACCTTCTGATCCATTTGTCAACTGATCAAGTTTATGAAGGGGTGAAGTCCTTTTACAAGGAAGATGGTGAAGTTGTTCCAGTAAATGTTTATGGGAAATCAAAAGTGGCAGCTGAGCAGTTCATTTCCGAGAAATGCTCAAACTTTGCAATTTTGAGAAGCAGTATCATCTTTGGGCCACAAACAATCTCACCGGTTTCAAAATCTCTTCCGATTCAGTGGGTTGATGGTGTCCTCTCCAAAGGAAATACAACGGAATTCTTTCATGATGAGTTTCGATGCCCTGTGTATGTAAAGGATGTCGTAGCAATCATACTTGCTTTGTCCAAGACATGGATATCAGAGGCTAAGCAAAGAAAATTGCTACTGAATGTTGGTGGACCAGACAGGGTATCCCGTGTACAAATGGCTGAGACGGTTGCTGATATAAGGGGACACAACCTCTCATTAATTAAATCTGTATCTGCATCATCGGTTGATCGTGGAGTTATGTCTCCTGCTGACATATCCATGGATATAACTAAGCTAGTTCAGACGCTTGGTATTTCTCCTATTTCATTTCGAGATGGTGTCAGATTGACGCTTGAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RmlD_sub_bind

Protein Analysis

256

Amino Acids

28.04

Weight (kDa)

5.7

Isoelectric Point (pI)

40.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 13 - 254 1.3e-35 RmlD substrate binding domain
Epimerase PF01370 13 - 169 2.5e-12 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 13 - 110 2.1e-07 GDP-mannose 4,6 dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 510
AclWI GGATC 3 cut(s) 89, 102, 181
AcsI RAATTY 1 cut(s) 434
AfaI GTAC 1 cut(s) 579
AfiI CCNNNNNNNGG 1 cut(s) 510
AgeI ACCGGT 1 cut(s) 370
AgsI TTSAA 3 cut(s) 10, 378, 764
AluBI AGCT 4 cut(s) 107, 153, 290, 703
AluI AGCT 4 cut(s) 107, 153, 290, 703
Alw26I GTCTC 3 cut(s) 155, 584, 675
AlwI GGATC 3 cut(s) 89, 102, 181
AoxI GGCC 1 cut(s) 353
ApeKI GCWGC 4 cut(s) 62, 101, 104, 287
ApoI RAATTY 1 cut(s) 434
AseI ATTAAT 1 cut(s) 626
AsiGI ACCGGT 1 cut(s) 370
Asp700I GAANNNNTTC 2 cut(s) 252, 438
AspS9I GGNCC 3 cut(s) 31, 353, 557
AsuHPI GGTGA 4 cut(s) 30, 232, 257, 360
AvaII GGWCC 2 cut(s) 31, 557
BamHI GGATCC 1 cut(s) 94
BarI GAAGNNNNNNTAC 2 cut(s) 215, 247
BauI CACGAG 1 cut(s) 78
BbvI GCAGC 4 cut(s) 49, 88, 116, 299
BccI CCATC 4 cut(s) 133, 236, 398, 737
BciVI GTATCC 1 cut(s) 580
BclI TGATCA 1 cut(s) 202
BcoDI GTCTC 3 cut(s) 155, 584, 675
BfaI CTAG 1 cut(s) 704
BfmI CTRYAG 1 cut(s) 102
BfuI GTATCC 1 cut(s) 580
BisI GCNGC 4 cut(s) 63, 102, 105, 288
BlpI GCTNAGC 2 cut(s) 291, 525
BlsI GCNGC 4 cut(s) 64, 103, 106, 289
Bme18I GGWCC 2 cut(s) 31, 557
BmgT120I GGNCC 3 cut(s) 31, 353, 557
BmiI GGNNCC 2 cut(s) 75, 96
BmsI GCATC 2 cut(s) 446, 653
BplI GAGNNNNNCTC 2 cut(s) 299, 331
Bpu1102I GCTNAGC 2 cut(s) 291, 525
BpuEI CTTGAG 1 cut(s) 150
BsaJI CCNNGG 1 cut(s) 687
BsaWI WCCGGW 2 cut(s) 33, 370
Bsc4I CCNNNNNNNGG 1 cut(s) 510
Bse118I RCCGGY 1 cut(s) 370
Bse1I ACTGG 1 cut(s) 257
BseDI CCNNGG 1 cut(s) 687
BseGI GGATG 1 cut(s) 481
BseLI CCNNNNNNNGG 1 cut(s) 510
BseMII CTCAG 2 cut(s) 282, 579
BseNI ACTGG 1 cut(s) 257
BseXI GCAGC 4 cut(s) 49, 88, 116, 299
BsgI GTGCAG 1 cut(s) 48
BshFI GGCC 1 cut(s) 355
BshTI ACCGGT 1 cut(s) 370
BsiSI CCGG 2 cut(s) 34, 371
BslFI GGGAC 1 cut(s) 624
BslI CCNNNNNNNGG 1 cut(s) 510
BsmAI GTCTC 3 cut(s) 155, 584, 675
BsmBI CGTCTC 1 cut(s) 584
BsmFI GGGAC 1 cut(s) 624
BsnI GGCC 1 cut(s) 355
Bsp1407I TGTACA 1 cut(s) 577
Bsp143I GATC 4 cut(s) 94, 186, 202, 655
Bsp1720I GCTNAGC 2 cut(s) 291, 525
Bsp19I CCATGG 1 cut(s) 687
BspANI GGCC 1 cut(s) 355
BspCNI CTCAG 2 cut(s) 283, 580
BspHI TCATGA 1 cut(s) 442
BspLI GGNNCC 2 cut(s) 75, 96
BspMAI CTGCAG 1 cut(s) 106
BspPI GGATC 3 cut(s) 89, 102, 181
BsrFI RCCGGY 1 cut(s) 370
BsrGI TGTACA 1 cut(s) 577
BsrI ACTGG 1 cut(s) 257
BssAI RCCGGY 1 cut(s) 370
BssECI CCNNGG 1 cut(s) 687
BssMI GATC 4 cut(s) 94, 186, 202, 655
BssSI CACGAG 1 cut(s) 78
BssT1I CCWWGG 1 cut(s) 687
Bst2BI CACGAG 1 cut(s) 78
Bst4CI ACNGT 3 cut(s) 59, 595, 768
Bst6I CTCTTC 2 cut(s) 153, 390
BstAUI TGTACA 1 cut(s) 577
BstDEI CTNAG 5 cut(s) 154, 291, 525, 588, 699
BstDSI CCRYGG 1 cut(s) 687
BstEII GGTNACC 1 cut(s) 36
BstF5I GGATG 1 cut(s) 481
BstKTI GATC 4 cut(s) 97, 189, 205, 658
BstMAI GTCTC 3 cut(s) 155, 584, 675
BstMBI GATC 4 cut(s) 94, 186, 202, 655
BstPI GGTNACC 1 cut(s) 36
BstSFI CTRYAG 1 cut(s) 102
BstV1I GCAGC 4 cut(s) 49, 88, 116, 299
BstX2I RGATCY 1 cut(s) 94
BstYI RGATCY 1 cut(s) 94
BsuI GTATCC 1 cut(s) 580
BsuRI GGCC 1 cut(s) 355
BtgI CCRYGG 1 cut(s) 687
BtsCI GGATG 1 cut(s) 481
BtsIMutI CAGTG 1 cut(s) 401
CciI TCATGA 1 cut(s) 442
Cfr10I RCCGGY 1 cut(s) 370
Cfr13I GGNCC 3 cut(s) 31, 353, 557
CseI GACGC 2 cut(s) 721, 766
Csp6I GTAC 1 cut(s) 578
CspAI ACCGGT 1 cut(s) 370
CviAII CATG 3 cut(s) 443, 510, 688
CviJI RGCY 7 cut(s) 107, 153, 290, 355, 524, 587, 703
CviKI_1 RGCY 7 cut(s) 107, 153, 290, 355, 524, 587, 703
CviQI GTAC 1 cut(s) 578
DdeI CTNAG 5 cut(s) 154, 291, 525, 588, 699
DpnI GATC 4 cut(s) 96, 188, 204, 657
DpnII GATC 4 cut(s) 94, 186, 202, 655
Eam1104I CTCTTC 2 cut(s) 153, 390
EarI CTCTTC 2 cut(s) 153, 390
Eco130I CCWWGG 1 cut(s) 687
Eco32I GATATC 1 cut(s) 516
Eco47I GGWCC 2 cut(s) 31, 557
Eco91I GGTNACC 1 cut(s) 36
EcoO65I GGTNACC 1 cut(s) 36
EcoRI GAATTC 1 cut(s) 434
EcoRV GATATC 1 cut(s) 516
EcoT14I CCWWGG 1 cut(s) 687
ErhI CCWWGG 1 cut(s) 687
Esp3I CGTCTC 1 cut(s) 584
FaeI CATG 3 cut(s) 446, 513, 691
FaqI GGGAC 1 cut(s) 624
FatI CATG 3 cut(s) 442, 509, 687
FbaI TGATCA 1 cut(s) 202
Fnu4HI GCNGC 4 cut(s) 63, 102, 105, 288
FokI GGATG 1 cut(s) 488
Fsp4HI GCNGC 4 cut(s) 63, 102, 105, 288
FspBI CTAG 1 cut(s) 704
GluI GCNGC 4 cut(s) 63, 102, 105, 288
HaeIII GGCC 1 cut(s) 355
HapII CCGG 2 cut(s) 34, 371
HgaI GACGC 2 cut(s) 721, 766
Hin1II CATG 3 cut(s) 446, 513, 691
HincII GTYRAC 1 cut(s) 198
HindII GTYRAC 1 cut(s) 198
HinfI GANTC 2 cut(s) 163, 391
HpaII CCGG 2 cut(s) 34, 371
HphI GGTGA 4 cut(s) 30, 232, 257, 360
Hpy166II GTNNAC 4 cut(s) 55, 198, 557, 578
Hpy188I TCNGA 6 cut(s) 186, 307, 390, 520, 711, 752
Hpy188III TCNNGA 3 cut(s) 167, 443, 740
Hpy8I GTNNAC 4 cut(s) 55, 198, 557, 578
HpyAV CCTTC 2 cut(s) 191, 209
HpyCH4III ACNGT 3 cut(s) 59, 595, 768
HpyCH4V TGCA 4 cut(s) 65, 104, 326, 644
HpyF3I CTNAG 5 cut(s) 154, 291, 525, 588, 699
Hsp92II CATG 3 cut(s) 446, 513, 691
Ksp22I TGATCA 1 cut(s) 202
Kzo9I GATC 4 cut(s) 94, 186, 202, 655
Lsp1109I GCAGC 4 cut(s) 49, 88, 116, 299
LweI GCATC 2 cut(s) 446, 653
MaeI CTAG 1 cut(s) 704
MaeIII GTNAC 1 cut(s) 36
MalI GATC 4 cut(s) 96, 188, 204, 657
MboI GATC 4 cut(s) 94, 186, 202, 655
MboII GAAGA 4 cut(s) 120, 170, 251, 377
MflI RGATCY 1 cut(s) 94
MluCI AATT 7 cut(s) 5, 139, 175, 327, 434, 536, 627
MlyI GAGTC 1 cut(s) 157
MnlI CCTC 4 cut(s) 87, 422, 514, 629
MroXI GAANNNNTTC 2 cut(s) 252, 438
MseI TTAA 4 cut(s) 117, 138, 626, 630
MslI CAYNNNNRTG 1 cut(s) 686
MspA1I CMGCKG 1 cut(s) 290
MspI CCGG 2 cut(s) 34, 371
NcoI CCATGG 1 cut(s) 687
NdeII GATC 4 cut(s) 94, 186, 202, 655
NlaIII CATG 3 cut(s) 446, 513, 691
NlaIV GGNNCC 2 cut(s) 75, 96
NmuCI GTSAC 1 cut(s) 36
PacI TTAATTAA 1 cut(s) 630
PagI TCATGA 1 cut(s) 442
PdmI GAANNNNTTC 2 cut(s) 252, 438
PfeI GAWTC 1 cut(s) 391
PflMI CCANNNNNTGG 1 cut(s) 510
PinAI ACCGGT 1 cut(s) 370
PkrI GCNGC 4 cut(s) 64, 103, 106, 289
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
PshBI ATTAAT 1 cut(s) 626
PspEI GGTNACC 1 cut(s) 36
PspN4I GGNNCC 2 cut(s) 75, 96
PspPI GGNCC 3 cut(s) 31, 353, 557
PstI CTGCAG 1 cut(s) 106
PsuI RGATCY 1 cut(s) 94
PvuII CAGCTG 1 cut(s) 290
RsaI GTAC 1 cut(s) 579
RsaNI GTAC 1 cut(s) 578
RseI CAYNNNNRTG 1 cut(s) 686
SaqAI TTAA 4 cut(s) 117, 138, 626, 630
SatI GCNGC 4 cut(s) 63, 102, 105, 288
Sau3AI GATC 4 cut(s) 94, 186, 202, 655
Sau96I GGNCC 3 cut(s) 31, 353, 557
SchI GAGTC 1 cut(s) 157
SetI ASST 7 cut(s) 43, 109, 155, 183, 292, 621, 705
SfaNI GCATC 2 cut(s) 446, 653
SfcI CTRYAG 1 cut(s) 102
SinI GGWCC 2 cut(s) 31, 557
SmiMI CAYNNNNRTG 1 cut(s) 686
SmlI CTYRAG 1 cut(s) 165
SmoI CTYRAG 1 cut(s) 165
Sse9I AATT 7 cut(s) 5, 139, 175, 327, 434, 536, 627
SspMI CTAG 1 cut(s) 704
StyI CCWWGG 1 cut(s) 687
TaaI ACNGT 3 cut(s) 59, 595, 768
TaqI TCGA 3 cut(s) 149, 454, 739
TasI AATT 7 cut(s) 5, 139, 175, 327, 434, 536, 627
TatI WGTACW 1 cut(s) 577
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 4 cut(s) 117, 138, 626, 630
Tru9I TTAA 4 cut(s) 117, 138, 626, 630
TscAI CASTG 1 cut(s) 401
TseFI GTSAC 1 cut(s) 36
TseI GCWGC 4 cut(s) 62, 101, 104, 287
Tsp45I GTSAC 1 cut(s) 36
TspDTI ATGAA 4 cut(s) 228, 289, 431, 723
TspGWI ACGGA 1 cut(s) 446
TspRI CASTG 1 cut(s) 401
Van91I CCANNNNNTGG 1 cut(s) 510
VpaK11BI GGWCC 2 cut(s) 31, 557
VspI ATTAAT 1 cut(s) 626
XapI RAATTY 1 cut(s) 434
XmnI GAANNNNTTC 2 cut(s) 252, 438
XspI CTAG 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.