RLG00000028284

Methionine adenosyltransferase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001020
Physical Location & Seq
Reverse (-)
21011625 .. 21014331
2707 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028284

Sequence Viewer

Length: 951 bp
ATGAGTAGGAAGAGAGTTTTGATAGTTGGAGGAACAGGTTACTTGGGACAGCATGTATTGCAAGGCTTTTCAGAGATTCAAGAGACCACTCCTTGTGATCTGGCATTTACCCACCACTCAAGTCCTCCTCCCCAAGCTTTGCTCAATGCATTTCCTAGTGCGATGCCTTTCTCTGTCGATTTGAAGCCTGGCAATGGATTTGAAGCCATTTCTCAGCAGTTTGGTCCGCCTGATGTGGTAGTAAACTGTGCTGCACTATCGGTTCCTCGTGCCTGTGAAATGGATCCTGCTGCCGCTATGTCAGTTAATGTGCCATCTTCTCTTGTTAATTGGTTATCGAGCTTGGAAGAGAGTAATTATCTACTGATCCATTTGTCAACTGATCAAGTTTATGAAGGGGTGAAGTCTTTTTACAAGGAAGATGATGAAGTTGTTCCAGTAAATGTTTATGGGAAATCAAAAGTGGCAGCTGAGCGGTTCATTACTGAGAAATGCTCAAACTTTGCAATTTTGAGAAGCAGTATCATCTTTGGGCCACAGACAATCTCACCGGTTGCAAAATCTCTTCCGATTCAGTGGGTTGATGGTGTCCTCTCCAAAGGGAATACAACGGAATTCTTTCATGATGAGTTTCGATGCCCTGTGTATGTAAAGGATGTCGTAGCAATCATACTGGCTTTGTCCAAGACATGGATATCAGAGGCTAAGCAAAGAAAATTGCTACTGAATGTTGGTGGACCGGACAGGGTATCCCGTGTCCAAATGGCTGAGACAGTTGCTGATATAAGGGGATACAACCCCTCATTAATTAAATCTGTATCTGCATCATCGGTTGATCGTGGAGTTATGTCTCCAGCTGACATATCCATGGATATAACTAAGCTAGTTCAGACGCTTGGTATTTCTCCTATTTCATTTCGAGATGGTGTCAGATTGACGCTGGAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RmlD_sub_bind

Protein Analysis

317

Amino Acids

34.39

Weight (kDa)

5.78

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 5 - 314 3.6e-40 RmlD substrate binding domain
Epimerase PF01370 6 - 229 3.4e-18 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 7 - 167 2.5e-07 GDP-mannose 4,6 dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 690
AccBSI CCGCTC 1 cut(s) 475
AciI CCGC 3 cut(s) 227, 294, 475
AclWI GGATC 3 cut(s) 278, 291, 361
AcsI RAATTY 1 cut(s) 614
AfiI CCNNNNNNNGG 2 cut(s) 194, 690
AgeI ACCGGT 1 cut(s) 550
AgsI TTSAA 3 cut(s) 80, 184, 203
AjnI CCWGG 1 cut(s) 187
AluBI AGCT 5 cut(s) 137, 342, 470, 857, 883
AluI AGCT 5 cut(s) 137, 342, 470, 857, 883
Alw26I GTCTC 3 cut(s) 77, 764, 855
AlwI GGATC 3 cut(s) 278, 291, 361
AlwNI CAGNNNCTG 1 cut(s) 779
AoxI GGCC 1 cut(s) 533
ApeKI GCWGC 3 cut(s) 251, 290, 467
ApoI RAATTY 1 cut(s) 614
AseI ATTAAT 1 cut(s) 806
AsiGI ACCGGT 1 cut(s) 550
Asp700I GAANNNNTTC 2 cut(s) 432, 618
AspS9I GGNCC 3 cut(s) 224, 533, 737
AsuHPI GGTGA 2 cut(s) 412, 540
AvaII GGWCC 2 cut(s) 224, 737
BamHI GGATCC 1 cut(s) 283
BarI GAAGNNNNNNTAC 2 cut(s) 395, 427
BauI CACGAG 1 cut(s) 267
BbvI GCAGC 3 cut(s) 238, 277, 479
BccI CCATC 3 cut(s) 322, 578, 917
BciT130I CCWGG 1 cut(s) 189
BciVI GTATCC 2 cut(s) 760, 785
BclI TGATCA 1 cut(s) 382
BcoDI GTCTC 3 cut(s) 77, 764, 855
BfaI CTAG 2 cut(s) 156, 884
BfuI GTATCC 2 cut(s) 760, 785
BisI GCNGC 4 cut(s) 252, 291, 294, 468
BlpI GCTNAGC 2 cut(s) 471, 705
BlsI GCNGC 4 cut(s) 253, 292, 295, 469
Bme1390I CCNGG 1 cut(s) 189
Bme18I GGWCC 2 cut(s) 224, 737
BmgT120I GGNCC 3 cut(s) 224, 533, 737
BmiI GGNNCC 2 cut(s) 264, 285
BmrFI CCNGG 1 cut(s) 189
BmsI GCATC 3 cut(s) 153, 626, 833
BplI GAGNNNNNCTC 2 cut(s) 479, 511
BpmI CTGGAG 1 cut(s) 837
Bpu1102I GCTNAGC 2 cut(s) 471, 705
BpuEI CTTGAG 1 cut(s) 103
BsaI GGTCTC 1 cut(s) 77
BsaJI CCNNGG 1 cut(s) 867
BsaWI WCCGGW 2 cut(s) 550, 739
Bsc4I CCNNNNNNNGG 2 cut(s) 194, 690
Bse118I RCCGGY 1 cut(s) 550
Bse1I ACTGG 2 cut(s) 437, 678
Bse3DI GCAATG 1 cut(s) 199
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 867
BseGI GGATG 1 cut(s) 661
BseLI CCNNNNNNNGG 2 cut(s) 194, 690
BseMI GCAATG 1 cut(s) 199
BseMII CTCAG 4 cut(s) 227, 462, 477, 759
BseNI ACTGG 2 cut(s) 437, 678
BseRI GAGGAG 1 cut(s) 117
BseXI GCAGC 3 cut(s) 238, 277, 479
BsgI GTGCAG 1 cut(s) 237
BshFI GGCC 1 cut(s) 535
BshTI ACCGGT 1 cut(s) 550
BsiSI CCGG 2 cut(s) 551, 740
BslFI GGGAC 1 cut(s) 60
BslI CCNNNNNNNGG 2 cut(s) 194, 690
BsmAI GTCTC 3 cut(s) 77, 764, 855
BsmFI GGGAC 1 cut(s) 60
BsnI GGCC 1 cut(s) 535
Bso31I GGTCTC 1 cut(s) 77
Bsp143I GATC 5 cut(s) 97, 283, 366, 382, 835
Bsp1720I GCTNAGC 2 cut(s) 471, 705
Bsp19I CCATGG 1 cut(s) 867
BspACI CCGC 3 cut(s) 227, 294, 475
BspANI GGCC 1 cut(s) 535
BspCNI CTCAG 4 cut(s) 226, 463, 478, 760
BspHI TCATGA 1 cut(s) 622
BspLI GGNNCC 2 cut(s) 264, 285
BspPI GGATC 3 cut(s) 278, 291, 361
BspTNI GGTCTC 1 cut(s) 77
BsrBI CCGCTC 1 cut(s) 475
BsrDI GCAATG 1 cut(s) 199
BsrFI RCCGGY 1 cut(s) 550
BsrI ACTGG 2 cut(s) 437, 678
BssAI RCCGGY 1 cut(s) 550
BssECI CCNNGG 1 cut(s) 867
BssMI GATC 5 cut(s) 97, 283, 366, 382, 835
BssSI CACGAG 1 cut(s) 267
BssT1I CCWWGG 1 cut(s) 867
Bst2BI CACGAG 1 cut(s) 267
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 3 cut(s) 248, 775, 948
Bst6I CTCTTC 3 cut(s) 5, 342, 570
BstAPI GCANNNNNTGC 1 cut(s) 58
BstDEI CTNAG 6 cut(s) 213, 471, 486, 705, 768, 879
BstDSI CCRYGG 1 cut(s) 867
BstF5I GGATG 1 cut(s) 661
BstKTI GATC 5 cut(s) 100, 286, 369, 385, 838
BstMAI GTCTC 3 cut(s) 77, 764, 855
BstMBI GATC 5 cut(s) 97, 283, 366, 382, 835
BstMWI GCNNNNNNNGC 1 cut(s) 58
BstNI CCWGG 1 cut(s) 189
BstNSI RCATGY 1 cut(s) 56
BstSCI CCNGG 1 cut(s) 187
BstV1I GCAGC 3 cut(s) 238, 277, 479
BstX2I RGATCY 1 cut(s) 283
BstYI RGATCY 1 cut(s) 283
BsuI GTATCC 2 cut(s) 760, 785
BsuRI GGCC 1 cut(s) 535
BtgI CCRYGG 1 cut(s) 867
BtgZI GCGATG 1 cut(s) 176
BtsCI GGATG 1 cut(s) 661
BtsIMutI CAGTG 1 cut(s) 581
CaiI CAGNNNCTG 1 cut(s) 779
CciI TCATGA 1 cut(s) 622
Cfr10I RCCGGY 1 cut(s) 550
Cfr13I GGNCC 3 cut(s) 224, 533, 737
CseI GACGC 2 cut(s) 901, 946
CspAI ACCGGT 1 cut(s) 550
CviAII CATG 4 cut(s) 53, 623, 690, 868
DdeI CTNAG 6 cut(s) 213, 471, 486, 705, 768, 879
DpnI GATC 5 cut(s) 99, 285, 368, 384, 837
DpnII GATC 5 cut(s) 97, 283, 366, 382, 835
Eam1104I CTCTTC 3 cut(s) 5, 342, 570
EarI CTCTTC 3 cut(s) 5, 342, 570
EciI GGCGGA 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 867
Eco31I GGTCTC 1 cut(s) 77
Eco32I GATATC 1 cut(s) 696
Eco47I GGWCC 2 cut(s) 224, 737
EcoRI GAATTC 1 cut(s) 614
EcoRII CCWGG 1 cut(s) 187
EcoRV GATATC 1 cut(s) 696
EcoT14I CCWWGG 1 cut(s) 867
EcoT22I ATGCAT 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 867
FaeI CATG 4 cut(s) 56, 626, 693, 871
FaqI GGGAC 1 cut(s) 60
FatI CATG 4 cut(s) 52, 622, 689, 867
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 4 cut(s) 252, 291, 294, 468
FokI GGATG 1 cut(s) 668
Fsp4HI GCNGC 4 cut(s) 252, 291, 294, 468
FspBI CTAG 2 cut(s) 156, 884
GluI GCNGC 4 cut(s) 252, 291, 294, 468
GsuI CTGGAG 1 cut(s) 837
HaeIII GGCC 1 cut(s) 535
HapII CCGG 2 cut(s) 551, 740
HgaI GACGC 2 cut(s) 901, 946
Hin1II CATG 4 cut(s) 56, 626, 693, 871
HincII GTYRAC 1 cut(s) 378
HindII GTYRAC 1 cut(s) 378
HindIII AAGCTT 1 cut(s) 135
HinfI GANTC 2 cut(s) 76, 571
HpaII CCGG 2 cut(s) 551, 740
HphI GGTGA 2 cut(s) 412, 540
Hpy166II GTNNAC 3 cut(s) 244, 378, 737
Hpy188I TCNGA 5 cut(s) 73, 570, 700, 891, 932
Hpy188III TCNNGA 3 cut(s) 80, 623, 920
Hpy8I GTNNAC 3 cut(s) 244, 378, 737
HpyAV CCTTC 1 cut(s) 389
HpyCH4III ACNGT 3 cut(s) 248, 775, 948
HpyCH4V TGCA 6 cut(s) 61, 149, 254, 506, 557, 824
HpyF10VI GCNNNNNNNGC 1 cut(s) 58
HpyF3I CTNAG 6 cut(s) 213, 471, 486, 705, 768, 879
Hsp92II CATG 4 cut(s) 56, 626, 693, 871
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 5 cut(s) 97, 283, 366, 382, 835
Lsp1109I GCAGC 3 cut(s) 238, 277, 479
LweI GCATC 3 cut(s) 153, 626, 833
MaeI CTAG 2 cut(s) 156, 884
MaeIII GTNAC 1 cut(s) 38
MalI GATC 5 cut(s) 99, 285, 368, 384, 837
MbiI CCGCTC 1 cut(s) 475
MboI GATC 5 cut(s) 97, 283, 366, 382, 835
MboII GAAGA 5 cut(s) 22, 309, 359, 431, 557
MflI RGATCY 1 cut(s) 283
MluCI AATT 6 cut(s) 328, 355, 507, 614, 716, 807
MmeI TCCRAC 1 cut(s) 7
MnlI CCTC 7 cut(s) 23, 135, 138, 276, 602, 694, 811
Mph1103I ATGCAT 1 cut(s) 151
MroXI GAANNNNTTC 2 cut(s) 432, 618
MseI TTAA 4 cut(s) 306, 327, 806, 810
MslI CAYNNNNRTG 1 cut(s) 866
MspA1I CMGCKG 2 cut(s) 470, 857
MspI CCGG 2 cut(s) 551, 740
MspR9I CCNGG 1 cut(s) 189
MvaI CCWGG 1 cut(s) 189
MwoI GCNNNNNNNGC 1 cut(s) 58
NcoI CCATGG 1 cut(s) 867
NdeII GATC 5 cut(s) 97, 283, 366, 382, 835
NlaIII CATG 4 cut(s) 56, 626, 693, 871
NlaIV GGNNCC 2 cut(s) 264, 285
NsiI ATGCAT 1 cut(s) 151
NspI RCATGY 1 cut(s) 56
PacI TTAATTAA 1 cut(s) 810
PagI TCATGA 1 cut(s) 622
PdmI GAANNNNTTC 2 cut(s) 432, 618
PfeI GAWTC 2 cut(s) 76, 571
PflMI CCANNNNNTGG 1 cut(s) 690
PinAI ACCGGT 1 cut(s) 550
PkrI GCNGC 4 cut(s) 253, 292, 295, 469
PshBI ATTAAT 1 cut(s) 806
Psp6I CCWGG 1 cut(s) 187
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 2 cut(s) 264, 285
PspPI GGNCC 3 cut(s) 224, 533, 737
PstNI CAGNNNCTG 1 cut(s) 779
PsuI RGATCY 1 cut(s) 283
PvuII CAGCTG 2 cut(s) 470, 857
RseI CAYNNNNRTG 1 cut(s) 866
SaqAI TTAA 4 cut(s) 306, 327, 806, 810
SatI GCNGC 4 cut(s) 252, 291, 294, 468
Sau3AI GATC 5 cut(s) 97, 283, 366, 382, 835
Sau96I GGNCC 3 cut(s) 224, 533, 737
ScrFI CCNGG 1 cut(s) 189
SetI ASST 6 cut(s) 40, 139, 344, 472, 859, 885
SfaNI GCATC 3 cut(s) 153, 626, 833
SinI GGWCC 2 cut(s) 224, 737
SmiMI CAYNNNNRTG 1 cut(s) 866
SmlI CTYRAG 1 cut(s) 118
SmoI CTYRAG 1 cut(s) 118
Sse9I AATT 6 cut(s) 328, 355, 507, 614, 716, 807
SsiI CCGC 3 cut(s) 227, 294, 475
SspMI CTAG 2 cut(s) 156, 884
StyD4I CCNGG 1 cut(s) 187
StyI CCWWGG 1 cut(s) 867
TaaI ACNGT 3 cut(s) 248, 775, 948
TaqI TCGA 4 cut(s) 177, 338, 634, 919
TasI AATT 6 cut(s) 328, 355, 507, 614, 716, 807
TauI GCSGC 1 cut(s) 296
TfiI GAWTC 2 cut(s) 76, 571
Tru1I TTAA 4 cut(s) 306, 327, 806, 810
Tru9I TTAA 4 cut(s) 306, 327, 806, 810
TscAI CASTG 1 cut(s) 581
TseI GCWGC 3 cut(s) 251, 290, 467
TspDTI ATGAA 5 cut(s) 408, 441, 469, 611, 903
TspGWI ACGGA 1 cut(s) 626
TspRI CASTG 1 cut(s) 581
Van91I CCANNNNNTGG 1 cut(s) 690
VpaK11BI GGWCC 2 cut(s) 224, 737
VspI ATTAAT 1 cut(s) 806
XapI RAATTY 1 cut(s) 614
XceI RCATGY 1 cut(s) 56
XmnI GAANNNNTTC 2 cut(s) 432, 618
XspI CTAG 2 cut(s) 156, 884
Zsp2I ATGCAT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.