RchiOBHm_Chr1g0353211

Methionine adenosyltransferase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001020
Physical Location & Seq
Forward (+)
46488735 .. 46490503
1769 bp
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UTR
Exon/CDS
Intron
PRQ57886

Sequence Viewer

Length: 378 bp
ATGAGCTTTAGAAACCCATATGATCAAATAAGAAACCATTTATCAATTCATGATGAGTTTCGATGCCCTGTTTATGTAAAGGACGTCGTACCAATCATCCTTGCTTTGTCCAAGACATGGATATCAGAGGCTAAGCATAGAAAATCGCTACTGAATGTTGGTGGACCAGACAGGTTATCCCGTGTTCAAATGGCTGAGACGGTTGCTGATATAAGGGGATACAACCTCTCATTAATTAAATCTGTATCTGCATCATCGGTTGATCACGGAGTTGTGTCTCCTGCTGACATATCCATGGATATATCTAAGCTAGTTCAGACGCCTGGTATTTCTCCAATTTCATTTGGAAATGGTGTCAGATTGACGCTTGAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RmlD_sub_bind

Protein Analysis

125

Amino Acids

13.79

Weight (kDa)

8.85

Isoelectric Point (pI)

26.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 16 - 123 7.2e-07 RmlD substrate binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 87
AccB7I CCANNNNNTGG 1 cut(s) 117
AcyI GRCGYC 2 cut(s) 84, 320
AfaI GTAC 1 cut(s) 90
AfiI CCNNNNNNNGG 1 cut(s) 117
AgsI TTSAA 2 cut(s) 188, 371
AjnI CCWGG 1 cut(s) 322
AluBI AGCT 2 cut(s) 6, 310
AluI AGCT 2 cut(s) 6, 310
Alw26I GTCTC 2 cut(s) 191, 282
AseI ATTAAT 1 cut(s) 233
AspS9I GGNCC 1 cut(s) 164
AvaII GGWCC 1 cut(s) 164
BciT130I CCWGG 1 cut(s) 324
BciVI GTATCC 1 cut(s) 212
BclI TGATCA 2 cut(s) 22, 262
BcoDI GTCTC 2 cut(s) 191, 282
BfaI CTAG 1 cut(s) 311
BfuI GTATCC 1 cut(s) 212
BlpI GCTNAGC 1 cut(s) 132
Bme1390I CCNGG 1 cut(s) 324
Bme18I GGWCC 1 cut(s) 164
BmgT120I GGNCC 1 cut(s) 164
BmrFI CCNGG 1 cut(s) 324
BmsI GCATC 2 cut(s) 53, 260
Bpu1102I GCTNAGC 1 cut(s) 132
BsaHI GRCGYC 2 cut(s) 84, 320
BsaJI CCNNGG 1 cut(s) 294
Bsc4I CCNNNNNNNGG 1 cut(s) 117
BseBI CCWGG 1 cut(s) 324
BseDI CCNNGG 1 cut(s) 294
BseGI GGATG 1 cut(s) 96
BseLI CCNNNNNNNGG 1 cut(s) 117
BseMII CTCAG 1 cut(s) 186
BslI CCNNNNNNNGG 1 cut(s) 117
BsmAI GTCTC 2 cut(s) 191, 282
BsmBI CGTCTC 1 cut(s) 191
Bsp143I GATC 2 cut(s) 22, 262
Bsp1720I GCTNAGC 1 cut(s) 132
Bsp19I CCATGG 1 cut(s) 294
BspCNI CTCAG 1 cut(s) 187
BspHI TCATGA 1 cut(s) 49
BssECI CCNNGG 1 cut(s) 294
BssMI GATC 2 cut(s) 22, 262
BssNI GRCGYC 2 cut(s) 84, 320
BssT1I CCWWGG 1 cut(s) 294
Bst2UI CCWGG 1 cut(s) 324
Bst4CI ACNGT 1 cut(s) 202
BstACI GRCGYC 2 cut(s) 84, 320
BstDEI CTNAG 3 cut(s) 132, 195, 306
BstDSI CCRYGG 1 cut(s) 294
BstF5I GGATG 1 cut(s) 96
BstKTI GATC 2 cut(s) 25, 265
BstMAI GTCTC 2 cut(s) 191, 282
BstMBI GATC 2 cut(s) 22, 262
BstNI CCWGG 1 cut(s) 324
BstSCI CCNGG 1 cut(s) 322
BsuI GTATCC 1 cut(s) 212
BtgI CCRYGG 1 cut(s) 294
BtsCI GGATG 1 cut(s) 96
CciI TCATGA 1 cut(s) 49
Cfr13I GGNCC 1 cut(s) 164
CseI GACGC 2 cut(s) 328, 373
Csp6I GTAC 1 cut(s) 89
CviAII CATG 3 cut(s) 50, 117, 295
CviJI RGCY 4 cut(s) 6, 131, 194, 310
CviKI_1 RGCY 4 cut(s) 6, 131, 194, 310
CviQI GTAC 1 cut(s) 89
DdeI CTNAG 3 cut(s) 132, 195, 306
DpnI GATC 2 cut(s) 24, 264
DpnII GATC 2 cut(s) 22, 262
Eco130I CCWWGG 1 cut(s) 294
Eco32I GATATC 1 cut(s) 123
Eco47I GGWCC 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 322
EcoRV GATATC 1 cut(s) 123
EcoT14I CCWWGG 1 cut(s) 294
ErhI CCWWGG 1 cut(s) 294
Esp3I CGTCTC 1 cut(s) 191
FaeI CATG 3 cut(s) 53, 120, 298
FatI CATG 3 cut(s) 49, 116, 294
FauNDI CATATG 1 cut(s) 19
FbaI TGATCA 2 cut(s) 22, 262
FokI GGATG 1 cut(s) 83
FspBI CTAG 1 cut(s) 311
HgaI GACGC 2 cut(s) 328, 373
Hin1I GRCGYC 2 cut(s) 84, 320
Hin1II CATG 3 cut(s) 53, 120, 298
Hpy166II GTNNAC 1 cut(s) 164
Hpy188I TCNGA 3 cut(s) 127, 318, 359
Hpy188III TCNNGA 1 cut(s) 50
Hpy8I GTNNAC 1 cut(s) 164
Hpy99I CGWCG 1 cut(s) 89
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 1 cut(s) 251
HpyF3I CTNAG 3 cut(s) 132, 195, 306
HpySE526I ACGT 1 cut(s) 84
Hsp92I GRCGYC 2 cut(s) 84, 320
Hsp92II CATG 3 cut(s) 53, 120, 298
Ksp22I TGATCA 2 cut(s) 22, 262
Kzo9I GATC 2 cut(s) 22, 262
LpnPI CCDG 6 cut(s) 81, 157, 180, 294, 309, 336
LweI GCATC 2 cut(s) 53, 260
MaeI CTAG 1 cut(s) 311
MaeII ACGT 1 cut(s) 84
MalI GATC 2 cut(s) 24, 264
MboI GATC 2 cut(s) 22, 262
MluCI AATT 4 cut(s) 45, 234, 336, 371
MnlI CCTC 2 cut(s) 121, 236
MseI TTAA 2 cut(s) 233, 237
MslI CAYNNNNRTG 1 cut(s) 293
MspR9I CCNGG 1 cut(s) 324
MvaI CCWGG 1 cut(s) 324
NcoI CCATGG 1 cut(s) 294
NdeI CATATG 1 cut(s) 19
NdeII GATC 2 cut(s) 22, 262
NlaIII CATG 3 cut(s) 53, 120, 298
PacI TTAATTAA 1 cut(s) 237
PagI TCATGA 1 cut(s) 49
PflMI CCANNNNNTGG 1 cut(s) 117
PshBI ATTAAT 1 cut(s) 233
Psp6I CCWGG 1 cut(s) 322
PspGI CCWGG 1 cut(s) 322
PspPI GGNCC 1 cut(s) 164
RsaI GTAC 1 cut(s) 90
RsaNI GTAC 1 cut(s) 89
RseI CAYNNNNRTG 1 cut(s) 293
SaqAI TTAA 2 cut(s) 233, 237
Sau3AI GATC 2 cut(s) 22, 262
Sau96I GGNCC 1 cut(s) 164
ScrFI CCNGG 1 cut(s) 324
SetI ASST 5 cut(s) 8, 87, 176, 228, 312
SfaNI GCATC 2 cut(s) 53, 260
SinI GGWCC 1 cut(s) 164
SmiMI CAYNNNNRTG 1 cut(s) 293
Sse9I AATT 4 cut(s) 45, 234, 336, 371
SspMI CTAG 1 cut(s) 311
StyD4I CCNGG 1 cut(s) 322
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 1 cut(s) 202
TaiI ACGT 1 cut(s) 87
TaqI TCGA 1 cut(s) 61
TasI AATT 4 cut(s) 45, 234, 336, 371
Tru1I TTAA 2 cut(s) 233, 237
Tru9I TTAA 2 cut(s) 233, 237
TspDTI ATGAA 2 cut(s) 38, 330
TspGWI ACGGA 1 cut(s) 282
Van91I CCANNNNNTGG 1 cut(s) 117
VpaK11BI GGWCC 1 cut(s) 164
VspI ATTAAT 1 cut(s) 233
XspI CTAG 1 cut(s) 311
ZraI GACGTC 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.