Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0001020

Physical Location & Seq

Genomic Coordinates Forward (+)

18400954 .. 18404495

Gene Structure 3542 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1128800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 978 bp

ATGACGAGTAAGAAGAGAGTTCTGGTAGTTGGGGGAACAGGTTACTTGGGCCAGCATGTACTGCAAGGGTTCTCACAGATTGAAGGGAAAGGGATCACATCAACCTCCCCTTTCGATCTGGCCTTTACCCACCACTCCAATCCTCCCCCCCAGGCGTTGCTCGACGCACATTTGCTGCCTTTCCAAGTCGATTTGAAGACCGGCCATGGATTTCAAGCCATTTCTCAGACGTTTGGCCGGCCTGATGTGGTCATAAACTGCGCTGCACTATCCGTACCTCGTGCCTGTGAAATGGATCCTGCTGCTGCTATGTCTGTGAATGTTCCATCTTCTCTTGTGAACTGGTTATCAAGCTTTGAAGAGAGTAGTTCTCTTCTGATCCATTTGTCAACTGATCAAGTTTATGAAGGGGTGAAGTCCTTTTACAAGGAAGATGATGAAACTGCTCCCGTAAATGTATATGGGAAATCGAAAGTGGCAGCAGAGCAGTTCATATCCGAAAAATGCTCAAACTTTGCAATTTTGAGAAGCAGCATCATCTTTGGGCCACAGACAATCTCACCGGTTCCAAAATCTCTTCCAATCCAGTGGATAGATGGTGTCCTCTCCAAAGGAAAGACGAGCGAGTTTTTCCATGACGAGTTCCGGTGTCCAGTGTATGTTAAAGATGTTGTAGCTGTCATATTAGCTCTGTCCAAGGGATGGATATATGAAAGTAAGCAAACAAAGGTGCTACTAAATGTCGGTGGACCAGATAGGGTATCCCGCCTTCAAATGGCTGAGATTGTTGCCAATGTTAGAGGATACAACCCCTCATTAATCAAATCAGTGTCTGCATCATCAGTCGATCGTGGAGTAATGTCTCCTGCCGACATATCCATGGATATAACTAAGCTGGTTCGGACACTTGGTATTGCTCCAACTTCATTTAGAGACGGTGTCAGATTGACGCTTGAAACTGAGGCTAAGTCGTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RmlD_sub_bind

Protein Analysis

326

Amino Acids

35.23

Weight (kDa)

7.05

Isoelectric Point (pI)

42.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RmlD_sub_bind	PF04321	6 - 320	2.1e-41	RmlD substrate binding domain
Epimerase	PF01370	7 - 231	2.8e-19	NAD dependent epimerase/dehydratase family
Polysacc_synt_2	PF02719	7 - 167	7.5e-06	Polysaccharide biosynthesis protein
GDP_Man_Dehyd	PF16363	8 - 179	6.1e-11	GDP-mannose 4,6 dehydratase
NAD_binding_4	PF07993	79 - 213	6.4e-06	Male sterility protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001020)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00560 AT4G00560 AT4G00560 AT4G00560
fragaria_vesca	FvH4_7g13560
malus_domestica	MD01G1052100.v1.1 MD07G1128800.v1.1
prunus_persica	Prupe.2G159800_v2.0.a1 Prupe.2G159800_v2.0.a1
pyrus_communis	pycom01g07780 pycom07g12450
rosa_chinensis	RchiOBHm_Chr0c39g0503211 RchiOBHm_Chr0c39g0503281 RchiOBHm_Chr0c39g0503291 RchiOBHm_Chr0c39g0503341 RchiOBHm_Chr1g0353111 RchiOBHm_Chr1g0353131 RchiOBHm_Chr1g0353141 RchiOBHm_Chr1g0353211 RchiOBHm_Chr1g0353241 RchiOBHm_Chr1g0353261 RchiOBHm_Chr1g0353281 RchiOBHm_Chr1g0353321 RchiOBHm_Chr1g0353331
rosa_laevigata	RLG00000009762 RLG00000028278 RLG00000028280 RLG00000028284 RLG00000028286 RLG00000028288
rosa_multiflora	Rmu_sc0001207.1_g000042 Rmu_sc0001207.1_g000081 Rmu_sc0011028.1_g000005
rosa_roxburghii	Rroxscaffold_4G00301960 Rroxscaffold_4G00301980 Rroxscaffold_4G00302000 Rroxscaffold_4G00302020 Rroxscaffold_4G00302040
rosa_rugosa	Rorug01G0233600 Rorug01G0233700 Rorug01G0233800 Rorug01G0234000 Rorug01G0234100 Rorug01G0234100 Rorug01G0234300 Rorug01G0235000
rosa_samantha	Rh1AG247600 Rh1BG020800 Rh1BG217400 Rh1BG217500 Rh1CG231100 Rh1DG243500 Rh1DG243800 Rh7CG311900
rosa_wichuraiana	Rw0G018080 Rw1G021400 Rw1G021430 Rw1G021450 Rw1G021460 Rw1G021530 Rw1G021550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	702
AciI	CCGC	1 cut(s)	766
AclWI	GGATC	4 cut(s)	101, 290, 303, 373
AcoI	YGGCCR	2 cut(s)	202, 235
AfaI	GTAC	2 cut(s)	60, 276
AfiI	CCNNNNNNNGG	2 cut(s)	702, 775
AgeI	ACCGGT	1 cut(s)	562
AgsI	TTSAA	6 cut(s)	83, 196, 215, 359, 773, 956
AjnI	CCWGG	1 cut(s)	150
AluBI	AGCT	4 cut(s)	354, 677, 689, 895
AluI	AGCT	4 cut(s)	354, 677, 689, 895
Alw26I	GTCTC	2 cut(s)	867, 927
AlwI	GGATC	4 cut(s)	101, 290, 303, 373
AlwNI	CAGNNNCTG	1 cut(s)	833
AoxI	GGCC	6 cut(s)	49, 120, 202, 235, 239, 545
ApeKI	GCWGC	6 cut(s)	175, 263, 302, 305, 479, 531
AseI	ATTAAT	1 cut(s)	818
AsiGI	ACCGGT	1 cut(s)	562
AspLEI	GCGC	1 cut(s)	263
AspS9I	GGNCC	3 cut(s)	49, 545, 749
AsuHPI	GGTGA	2 cut(s)	424, 552
AvaII	GGWCC	1 cut(s)	749
BamHI	GGATCC	1 cut(s)	295
BarI	GAAGNNNNNNTAC	4 cut(s)	407, 439, 753, 785
BauI	CACGAG	1 cut(s)	279
BbsI	GAAGAC	1 cut(s)	203
BbvI	GCAGC	6 cut(s)	162, 250, 289, 292, 491, 543
BccI	CCATC	3 cut(s)	334, 590, 696
BciT130I	CCWGG	1 cut(s)	152
BciVI	GTATCC	2 cut(s)	772, 797
BclI	TGATCA	1 cut(s)	394
BcoDI	GTCTC	2 cut(s)	867, 927
BfuI	GTATCC	2 cut(s)	772, 797
BisI	GCNGC	6 cut(s)	176, 264, 303, 306, 480, 532
BlsI	GCNGC	6 cut(s)	177, 265, 304, 307, 481, 533
Bme1390I	CCNGG	1 cut(s)	152
Bme18I	GGWCC	1 cut(s)	749
BmgT120I	GGNCC	3 cut(s)	49, 545, 749
BmiI	GGNNCC	2 cut(s)	297, 567
BmrFI	CCNGG	1 cut(s)	152
BmsI	GCATC	2 cut(s)	543, 845
BpiI	GAAGAC	1 cut(s)	203
BplI	GAGNNNNNCTC	2 cut(s)	355, 387
BsaJI	CCNNGG	4 cut(s)	150, 205, 696, 879
BsaWI	WCCGGW	2 cut(s)	562, 645
Bsc4I	CCNNNNNNNGG	2 cut(s)	702, 775
Bse118I	RCCGGY	3 cut(s)	200, 237, 562
Bse1I	ACTGG	3 cut(s)	347, 586, 653
BseBI	CCWGG	1 cut(s)	152
BseDI	CCNNGG	4 cut(s)	150, 205, 696, 879
BseGI	GGATG	1 cut(s)	707
BseLI	CCNNNNNNNGG	2 cut(s)	702, 775
BseMII	CTCAG	3 cut(s)	239, 771, 951
BseNI	ACTGG	3 cut(s)	347, 586, 653
BseXI	GCAGC	6 cut(s)	162, 250, 289, 292, 491, 543
BsgI	GTGCAG	1 cut(s)	249
Bsh1285I	CGRYCG	1 cut(s)	850
BshFI	GGCC	6 cut(s)	51, 122, 204, 237, 241, 547
BshTI	ACCGGT	1 cut(s)	562
BsiEI	CGRYCG	1 cut(s)	850
BsiSI	CCGG	4 cut(s)	201, 238, 563, 646
BslI	CCNNNNNNNGG	2 cut(s)	702, 775
BsmAI	GTCTC	2 cut(s)	867, 927
BsmBI	CGTCTC	1 cut(s)	927
BsnI	GGCC	6 cut(s)	51, 122, 204, 237, 241, 547
Bsp143I	GATC	6 cut(s)	93, 115, 295, 378, 394, 847
Bsp19I	CCATGG	2 cut(s)	205, 879
BspACI	CCGC	1 cut(s)	766
BspANI	GGCC	6 cut(s)	51, 122, 204, 237, 241, 547
BspCNI	CTCAG	3 cut(s)	238, 772, 952
BspLI	GGNNCC	2 cut(s)	297, 567
BspPI	GGATC	4 cut(s)	101, 290, 303, 373
BsrFI	RCCGGY	3 cut(s)	200, 237, 562
BsrI	ACTGG	3 cut(s)	347, 586, 653
BssAI	RCCGGY	3 cut(s)	200, 237, 562
BssECI	CCNNGG	4 cut(s)	150, 205, 696, 879
BssMI	GATC	6 cut(s)	93, 115, 295, 378, 394, 847
BssSI	CACGAG	1 cut(s)	279
BssT1I	CCWWGG	3 cut(s)	205, 696, 879
Bst2BI	CACGAG	1 cut(s)	279
Bst2UI	CCWGG	1 cut(s)	152
Bst4CI	ACNGT	1 cut(s)	938
Bst6I	CTCTTC	4 cut(s)	8, 354, 378, 582
BstAPI	GCANNNNNTGC	1 cut(s)	61
BstC8I	GCNNGC	2 cut(s)	53, 239
BstDEI	CTNAG	5 cut(s)	225, 780, 891, 960, 966
BstDSI	CCRYGG	2 cut(s)	205, 879
BstF5I	GGATG	1 cut(s)	707
BstHHI	GCGC	1 cut(s)	263
BstKTI	GATC	6 cut(s)	96, 118, 298, 381, 397, 850
BstMAI	GTCTC	2 cut(s)	867, 927
BstMBI	GATC	6 cut(s)	93, 115, 295, 378, 394, 847
BstMCI	CGRYCG	1 cut(s)	850
BstMWI	GCNNNNNNNGC	1 cut(s)	61
BstNI	CCWGG	1 cut(s)	152
BstNSI	RCATGY	1 cut(s)	59
BstSCI	CCNGG	1 cut(s)	150
BstV1I	GCAGC	6 cut(s)	162, 250, 289, 292, 491, 543
BstV2I	GAAGAC	1 cut(s)	203
BstX2I	RGATCY	1 cut(s)	295
BstXI	CCANNNNNNTGG	1 cut(s)	588
BstYI	RGATCY	1 cut(s)	295
BsuI	GTATCC	2 cut(s)	772, 797
BsuRI	GGCC	6 cut(s)	51, 122, 204, 237, 241, 547
BtgI	CCRYGG	2 cut(s)	205, 879
BtsCI	GGATG	1 cut(s)	707
BtsIMutI	CAGTG	3 cut(s)	593, 660, 834
Cac8I	GCNNGC	2 cut(s)	53, 239
CaiI	CAGNNNCTG	1 cut(s)	833
CfoI	GCGC	1 cut(s)	263
Cfr10I	RCCGGY	3 cut(s)	200, 237, 562
Cfr13I	GGNCC	3 cut(s)	49, 545, 749
CseI	GACGC	2 cut(s)	173, 958
Csp6I	GTAC	2 cut(s)	59, 275
CspAI	ACCGGT	1 cut(s)	562
CviAII	CATG	4 cut(s)	56, 206, 635, 880
CviQI	GTAC	2 cut(s)	59, 275
DdeI	CTNAG	5 cut(s)	225, 780, 891, 960, 966
DpnI	GATC	6 cut(s)	95, 117, 297, 380, 396, 849
DpnII	GATC	6 cut(s)	93, 115, 295, 378, 394, 847
EaeI	YGGCCR	2 cut(s)	202, 235
Eam1104I	CTCTTC	4 cut(s)	8, 354, 378, 582
EarI	CTCTTC	4 cut(s)	8, 354, 378, 582
Eco130I	CCWWGG	3 cut(s)	205, 696, 879
Eco47I	GGWCC	1 cut(s)	749
EcoRII	CCWGG	1 cut(s)	150
EcoT14I	CCWWGG	3 cut(s)	205, 696, 879
ErhI	CCWWGG	3 cut(s)	205, 696, 879
Esp3I	CGTCTC	1 cut(s)	927
FaeI	CATG	4 cut(s)	59, 209, 638, 883
FatI	CATG	4 cut(s)	55, 205, 634, 879
FauI	CCCGC	1 cut(s)	773
FbaI	TGATCA	1 cut(s)	394
Fnu4HI	GCNGC	6 cut(s)	176, 264, 303, 306, 480, 532
FokI	GGATG	1 cut(s)	714
FseI	GGCCGGCC	1 cut(s)	241
Fsp4HI	GCNGC	6 cut(s)	176, 264, 303, 306, 480, 532
GlaI	GCGC	1 cut(s)	262
GluI	GCNGC	6 cut(s)	176, 264, 303, 306, 480, 532
HaeIII	GGCC	6 cut(s)	51, 122, 204, 237, 241, 547
HapII	CCGG	4 cut(s)	201, 238, 563, 646
HgaI	GACGC	2 cut(s)	173, 958
HhaI	GCGC	1 cut(s)	263
Hin1II	CATG	4 cut(s)	59, 209, 638, 883
Hin6I	GCGC	1 cut(s)	261
HinP1I	GCGC	1 cut(s)	261
HincII	GTYRAC	1 cut(s)	390
HindII	GTYRAC	1 cut(s)	390
HindIII	AAGCTT	1 cut(s)	352
HpaII	CCGG	4 cut(s)	201, 238, 563, 646
HphI	GGTGA	2 cut(s)	424, 552
Hpy166II	GTNNAC	3 cut(s)	340, 390, 749
Hpy188I	TCNGA	5 cut(s)	228, 378, 499, 903, 944
Hpy188III	TCNNGA	1 cut(s)	975
Hpy8I	GTNNAC	3 cut(s)	340, 390, 749
Hpy99I	CGWCG	1 cut(s)	167
HpyAV	CCTTC	3 cut(s)	77, 401, 779
HpyCH4III	ACNGT	1 cut(s)	938
HpyCH4IV	ACGT	1 cut(s)	230
HpyCH4V	TGCA	4 cut(s)	64, 266, 518, 836
HpyF10VI	GCNNNNNNNGC	1 cut(s)	61
HpyF3I	CTNAG	5 cut(s)	225, 780, 891, 960, 966
HpySE526I	ACGT	1 cut(s)	230
Hsp92II	CATG	4 cut(s)	59, 209, 638, 883
HspAI	GCGC	1 cut(s)	261
KroI	GCCGGC	1 cut(s)	237
KroNI	GCCGGC	1 cut(s)	239
Ksp22I	TGATCA	1 cut(s)	394
Kzo9I	GATC	6 cut(s)	93, 115, 295, 378, 394, 847
LmnI	GCTCC	2 cut(s)	451, 922
Lsp1109I	GCAGC	6 cut(s)	162, 250, 289, 292, 491, 543
LweI	GCATC	2 cut(s)	543, 845
MaeII	ACGT	1 cut(s)	230
MaeIII	GTNAC	1 cut(s)	41
MalI	GATC	6 cut(s)	95, 117, 297, 380, 396, 849
MboI	GATC	6 cut(s)	93, 115, 295, 378, 394, 847
MboII	GAAGA	7 cut(s)	25, 208, 321, 365, 371, 443, 569
MflI	RGATCY	1 cut(s)	295
MluCI	AATT	1 cut(s)	519
MmeI	TCCRAC	1 cut(s)	944
MnlI	CCTC	7 cut(s)	115, 153, 288, 614, 794, 823, 955
MroNI	GCCGGC	1 cut(s)	237
MseI	TTAA	2 cut(s)	663, 818
MslI	CAYNNNNRTG	1 cut(s)	878
MspI	CCGG	4 cut(s)	201, 238, 563, 646
MspR9I	CCNGG	1 cut(s)	152
MvaI	CCWGG	1 cut(s)	152
MwoI	GCNNNNNNNGC	1 cut(s)	61
NaeI	GCCGGC	1 cut(s)	239
NcoI	CCATGG	2 cut(s)	205, 879
NdeII	GATC	6 cut(s)	93, 115, 295, 378, 394, 847
NgoMIV	GCCGGC	1 cut(s)	237
NlaIII	CATG	4 cut(s)	59, 209, 638, 883
NlaIV	GGNNCC	2 cut(s)	297, 567
NspI	RCATGY	1 cut(s)	59
PdiI	GCCGGC	1 cut(s)	239
PflFI	GACNNNGTC	1 cut(s)	938
PflMI	CCANNNNNTGG	1 cut(s)	702
PinAI	ACCGGT	1 cut(s)	562
PkrI	GCNGC	6 cut(s)	177, 265, 304, 307, 481, 533
Ple19I	CGATCG	1 cut(s)	850
PshBI	ATTAAT	1 cut(s)	818
Psp6I	CCWGG	1 cut(s)	150
PspGI	CCWGG	1 cut(s)	150
PspN4I	GGNNCC	2 cut(s)	297, 567
PspPI	GGNCC	3 cut(s)	49, 545, 749
PstNI	CAGNNNCTG	1 cut(s)	833
PsuI	RGATCY	1 cut(s)	295
PsyI	GACNNNGTC	1 cut(s)	938
PvuI	CGATCG	1 cut(s)	850
RigI	GGCCGGCC	1 cut(s)	241
RsaI	GTAC	2 cut(s)	60, 276
RsaNI	GTAC	2 cut(s)	59, 275
RseI	CAYNNNNRTG	1 cut(s)	878
SaqAI	TTAA	2 cut(s)	663, 818
SatI	GCNGC	6 cut(s)	176, 264, 303, 306, 480, 532
Sau3AI	GATC	6 cut(s)	93, 115, 295, 378, 394, 847
Sau96I	GGNCC	3 cut(s)	49, 545, 749
ScrFI	CCNGG	1 cut(s)	152
SetI	ASST	9 cut(s)	43, 107, 233, 280, 356, 679, 691, 732, 897
SfaNI	GCATC	2 cut(s)	543, 845
SinI	GGWCC	1 cut(s)	749
SmiMI	CAYNNNNRTG	1 cut(s)	878
Sse9I	AATT	1 cut(s)	519
SsiI	CCGC	1 cut(s)	766
StyD4I	CCNGG	1 cut(s)	150
StyI	CCWWGG	3 cut(s)	205, 696, 879
TaaI	ACNGT	1 cut(s)	938
TaiI	ACGT	1 cut(s)	233
TaqI	TCGA	5 cut(s)	114, 162, 189, 470, 846
TasI	AATT	1 cut(s)	519
TatI	WGTACW	1 cut(s)	58
Tru1I	TTAA	2 cut(s)	663, 818
Tru9I	TTAA	2 cut(s)	663, 818
TscAI	CASTG	3 cut(s)	593, 660, 834
TseI	GCWGC	6 cut(s)	175, 263, 302, 305, 479, 531
TspDTI	ATGAA	5 cut(s)	420, 453, 481, 726, 915
TspGWI	ACGGA	1 cut(s)	262
TspRI	CASTG	3 cut(s)	593, 660, 834
Tth111I	GACNNNGTC	1 cut(s)	938
Van91I	CCANNNNNTGG	1 cut(s)	702
VpaK11BI	GGWCC	1 cut(s)	749
VspI	ATTAAT	1 cut(s)	818
XceI	RCATGY	1 cut(s)	59
XcmI	CCANNNNNNNNNTGG	1 cut(s)	593

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.