Rh1DG243800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001020
Physical Location & Seq
Forward (+)
45907781 .. 45913758
5978 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG243800.1

Sequence Viewer

Length: 360 bp
ATGCTTCGGGCAAAGTGGACGAAGACCTGGGCTGACGAAGACCCGGCCCTGAGAATCAAATTCACACTGAGCTCTACAATCCCCTCAGTCAGAGAAAGAGAGTTGGTGATCGGAGCAAATGGGAGGAGGCGAGCATGCGCACGGCGGAGACTTCAGGGCGAAGGTGTGGAGCATGAGCGGTGGGCCCTACTGCAGGCCCAAGCACTGGAAGCGCAACACGGCCTTCGCCATGTTCGGAGTCTTCCTCATCTGCATTCCCATCGCCATGAAATCTGCCGAGCTCGAGGTTCGTTCATCTCACCTCAATTCATCTCCTTTCTCTTTTGGTTTCGACTTTGGATTTCTGTTTTAGAAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

119

Amino Acids

14.02

Weight (kDa)

11.35

Isoelectric Point (pI)

59.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 139
AccB7I CCANNNNNTGG 1 cut(s) 205
AccBSI CCGCTC 1 cut(s) 178
AciI CCGC 2 cut(s) 145, 178
AcsI RAATTY 1 cut(s) 59
AcuI CTGAAG 1 cut(s) 137
AfiI CCNNNNNNNGG 2 cut(s) 193, 205
AjnI CCWGG 1 cut(s) 26
AluBI AGCT 2 cut(s) 72, 281
AluI AGCT 2 cut(s) 72, 281
Alw21I GWGCWC 2 cut(s) 74, 283
Alw26I GTCTC 1 cut(s) 142
Ama87I CYCGRG 1 cut(s) 282
AoxI GGCC 4 cut(s) 45, 183, 195, 220
ApaI GGGCCC 1 cut(s) 187
ApoI RAATTY 1 cut(s) 59
AspLEI GCGC 2 cut(s) 140, 214
AspS9I GGNCC 4 cut(s) 46, 183, 184, 196
AsuC2I CCSGG 1 cut(s) 44
AsuHPI GGTGA 2 cut(s) 118, 291
AvaI CYCGRG 1 cut(s) 282
BaeGI GKGCMC 1 cut(s) 187
BanII GRGCYC 3 cut(s) 74, 187, 283
BbsI GAAGAC 3 cut(s) 29, 45, 233
Bbv12I GWGCWC 2 cut(s) 74, 283
BccI CCATC 1 cut(s) 267
BceAI ACGGC 2 cut(s) 158, 235
BcgI CGANNNNNNTGC 2 cut(s) 242, 276
BciT130I CCWGG 1 cut(s) 28
BcnI CCSGG 1 cut(s) 44
BcoDI GTCTC 1 cut(s) 142
BfmI CTRYAG 1 cut(s) 191
Bme1390I CCNGG 2 cut(s) 28, 44
BmeT110I CYCGRG 1 cut(s) 282
BmgT120I GGNCC 4 cut(s) 46, 183, 184, 196
BmiI GGNNCC 1 cut(s) 185
BmrFI CCNGG 2 cut(s) 28, 44
BpiI GAAGAC 3 cut(s) 29, 45, 233
BplI GAGNNNNNCTC 2 cut(s) 229, 261
BpuMI CCSGG 1 cut(s) 44
BsaJI CCNNGG 1 cut(s) 27
Bsc4I CCNNNNNNNGG 2 cut(s) 193, 205
Bse1I ACTGG 1 cut(s) 210
BseBI CCWGG 1 cut(s) 28
BseDI CCNNGG 1 cut(s) 27
BseLI CCNNNNNNNGG 2 cut(s) 193, 205
BseMII CTCAG 3 cut(s) 41, 59, 99
BseNI ACTGG 1 cut(s) 210
BseRI GAGGAG 1 cut(s) 139
BseSI GKGCMC 1 cut(s) 187
BshFI GGCC 4 cut(s) 47, 185, 197, 222
BsiHKAI GWGCWC 2 cut(s) 74, 283
BsiHKCI CYCGRG 1 cut(s) 282
BsiSI CCGG 1 cut(s) 44
BslI CCNNNNNNNGG 2 cut(s) 193, 205
BsmAI GTCTC 1 cut(s) 142
BsmI GAATGC 1 cut(s) 253
BsnI GGCC 4 cut(s) 47, 185, 197, 222
BsoBI CYCGRG 1 cut(s) 282
Bsp120I GGGCCC 1 cut(s) 183
Bsp1286I GDGCHC 3 cut(s) 74, 187, 283
Bsp143I GATC 1 cut(s) 108
BspACI CCGC 2 cut(s) 145, 178
BspANI GGCC 4 cut(s) 47, 185, 197, 222
BspCNI CTCAG 3 cut(s) 42, 60, 98
BspLI GGNNCC 1 cut(s) 185
BspMAI CTGCAG 1 cut(s) 195
BsrBI CCGCTC 1 cut(s) 178
BsrI ACTGG 1 cut(s) 210
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 1 cut(s) 108
Bst2UI CCWGG 1 cut(s) 28
BstC8I GCNNGC 3 cut(s) 132, 136, 195
BstDEI CTNAG 3 cut(s) 50, 68, 85
BstENI CCTNNNNNAGG 1 cut(s) 191
BstHHI GCGC 2 cut(s) 140, 214
BstKTI GATC 1 cut(s) 111
BstMAI GTCTC 1 cut(s) 142
BstMBI GATC 1 cut(s) 108
BstMWI GCNNNNNNNGC 1 cut(s) 209
BstNI CCWGG 1 cut(s) 28
BstNSI RCATGY 1 cut(s) 138
BstSCI CCNGG 2 cut(s) 26, 42
BstSFI CTRYAG 1 cut(s) 191
BstSLI GKGCMC 1 cut(s) 187
BstV2I GAAGAC 3 cut(s) 29, 45, 233
BsuRI GGCC 4 cut(s) 47, 185, 197, 222
BtgZI GCGATG 1 cut(s) 245
BtsIMutI CAGTG 2 cut(s) 65, 203
Cac8I GCNNGC 3 cut(s) 132, 136, 195
CfoI GCGC 2 cut(s) 140, 214
Cfr13I GGNCC 4 cut(s) 46, 183, 184, 196
CviAII CATG 4 cut(s) 135, 173, 230, 266
CviJI RGCY 7 cut(s) 32, 47, 72, 185, 197, 222, 281
CviKI_1 RGCY 7 cut(s) 32, 47, 72, 185, 197, 222, 281
DdeI CTNAG 3 cut(s) 50, 68, 85
DpnI GATC 1 cut(s) 110
DpnII GATC 1 cut(s) 108
EciI GGCGGA 1 cut(s) 160
Ecl136II GAGCTC 2 cut(s) 72, 281
Eco24I GRGCYC 3 cut(s) 74, 187, 283
Eco53kI GAGCTC 2 cut(s) 72, 281
Eco57I CTGAAG 1 cut(s) 137
Eco88I CYCGRG 1 cut(s) 282
EcoICRI GAGCTC 2 cut(s) 72, 281
EcoNI CCTNNNNNAGG 1 cut(s) 191
EcoO109I RGGNCCY 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 26
EcoT38I GRGCYC 3 cut(s) 74, 187, 283
FaeI CATG 4 cut(s) 138, 176, 233, 269
FaiI YATR 4 cut(s) 136, 174, 231, 267
FatI CATG 4 cut(s) 134, 172, 229, 265
FriOI GRGCYC 3 cut(s) 74, 187, 283
FspAI RTGCGCAY 1 cut(s) 139
FspI TGCGCA 1 cut(s) 139
GlaI GCGC 2 cut(s) 139, 213
HaeIII GGCC 4 cut(s) 47, 185, 197, 222
HapII CCGG 1 cut(s) 44
HhaI GCGC 2 cut(s) 140, 214
Hin1II CATG 4 cut(s) 138, 176, 233, 269
Hin6I GCGC 2 cut(s) 138, 212
HinP1I GCGC 2 cut(s) 138, 212
HinfI GANTC 2 cut(s) 54, 238
HpaII CCGG 1 cut(s) 44
HphI GGTGA 2 cut(s) 118, 291
Hpy166II GTNNAC 1 cut(s) 18
Hpy188I TCNGA 3 cut(s) 92, 113, 237
Hpy8I GTNNAC 1 cut(s) 18
HpyAV CCTTC 2 cut(s) 155, 233
HpyCH4V TGCA 2 cut(s) 193, 253
HpyF10VI GCNNNNNNNGC 1 cut(s) 209
HpyF3I CTNAG 3 cut(s) 50, 68, 85
Hsp92II CATG 4 cut(s) 138, 176, 233, 269
HspAI GCGC 2 cut(s) 138, 212
Kzo9I GATC 1 cut(s) 108
LmnI GCTCC 2 cut(s) 113, 169
LpnPI CCDG 7 cut(s) 13, 40, 57, 62, 140, 179, 191
MalI GATC 1 cut(s) 110
MbiI CCGCTC 1 cut(s) 178
MboI GATC 1 cut(s) 108
MboII GAAGA 3 cut(s) 34, 50, 233
MhlI GDGCHC 3 cut(s) 74, 187, 283
MluCI AATT 2 cut(s) 59, 305
MlyI GAGTC 1 cut(s) 247
MnlI CCTC 6 cut(s) 94, 117, 120, 255, 278, 312
MslI CAYNNNNRTG 1 cut(s) 264
MspI CCGG 1 cut(s) 44
MspR9I CCNGG 2 cut(s) 28, 44
Mva1269I GAATGC 1 cut(s) 253
MvaI CCWGG 1 cut(s) 28
MwoI GCNNNNNNNGC 1 cut(s) 209
NciI CCSGG 1 cut(s) 44
NdeII GATC 1 cut(s) 108
NlaIII CATG 4 cut(s) 138, 176, 233, 269
NlaIV GGNNCC 1 cut(s) 185
NmeAIII GCCGAG 1 cut(s) 302
NsbI TGCGCA 1 cut(s) 139
NspI RCATGY 1 cut(s) 138
PaeI GCATGC 1 cut(s) 138
PaeR7I CTCGAG 1 cut(s) 282
PctI GAATGC 1 cut(s) 253
PfeI GAWTC 1 cut(s) 54
PflMI CCANNNNNTGG 1 cut(s) 205
PleI GAGTC 1 cut(s) 246
PpsI GAGTC 1 cut(s) 246
Psp124BI GAGCTC 2 cut(s) 74, 283
Psp6I CCWGG 1 cut(s) 26
PspGI CCWGG 1 cut(s) 26
PspN4I GGNNCC 1 cut(s) 185
PspOMI GGGCCC 1 cut(s) 183
PspPI GGNCC 4 cut(s) 46, 183, 184, 196
PspXI VCTCGAGB 1 cut(s) 282
PstI CTGCAG 1 cut(s) 195
RseI CAYNNNNRTG 1 cut(s) 264
SacI GAGCTC 2 cut(s) 74, 283
Sau3AI GATC 1 cut(s) 108
Sau96I GGNCC 4 cut(s) 46, 183, 184, 196
SchI GAGTC 1 cut(s) 247
ScrFI CCNGG 2 cut(s) 28, 44
SduI GDGCHC 3 cut(s) 74, 187, 283
SetI ASST 6 cut(s) 29, 74, 166, 283, 289, 304
SfcI CTRYAG 1 cut(s) 191
Sfr274I CTCGAG 1 cut(s) 282
SlaI CTCGAG 1 cut(s) 282
SmiMI CAYNNNNRTG 1 cut(s) 264
SmlI CTYRAG 1 cut(s) 282
SmoI CTYRAG 1 cut(s) 282
SphI GCATGC 1 cut(s) 138
Sse9I AATT 2 cut(s) 59, 305
SsiI CCGC 2 cut(s) 145, 178
SstI GAGCTC 2 cut(s) 74, 283
StyD4I CCNGG 2 cut(s) 26, 42
TaqI TCGA 2 cut(s) 283, 331
TasI AATT 2 cut(s) 59, 305
TfiI GAWTC 1 cut(s) 54
TscAI CASTG 2 cut(s) 72, 210
TspDTI ATGAA 3 cut(s) 282, 283, 298
TspRI CASTG 2 cut(s) 72, 210
Van91I CCANNNNNTGG 1 cut(s) 205
XagI CCTNNNNNAGG 1 cut(s) 191
XapI RAATTY 1 cut(s) 59
XceI RCATGY 1 cut(s) 138
XhoI CTCGAG 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.