Rw0G018080

Methionine adenosyltransferase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00801
OG0001020
Physical Location & Seq
Reverse (-)
20530 .. 23290
2761 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G018080.1

Sequence Viewer

Length: 951 bp
ATGAGTAGGAAGAGAGTTTTGATAGTTGGAGGCACAGGTTACTTGGGACAGCATGTATTGCAAGGCTTTTCAGAGATTCAAGAAACCACTCCTTGTGATCTGGCATTCACCCACCACTCAAGTCCTCCTCCCCAAGCTTTGCTCAATGCATTTCCTAGTGTGATGCCTTTCTCTGTGGATTTGAAGTCTGGCAATGGATTTGAAGCCATTTCTCATTGGTTTGGTCCGCCTGATGTGGTAGTAAACTGTGCTGCACTTTCGGTTCCTCGTGCCTGTGAAATGGATCCTGCTGCAGCTATGTCAGTTAATGTGCCATCTTCTCTTGTTAATTGGTTATCGAGCTTAGAAGAGAGTAATTATCTTCTGATCCATTTGTCAACTGATCAAGTTTATGAAGGGGTGAAGTCTTTTTACAAGGAAGATGATGAAGTTGTTCCAGTAAATGTTTATGGGAAATCAAAAGTGGCAGCTGAGCAGTTCATTACTGAGAAATGCTCAAACTTTGCAATTTTGAGAAGCAGTATCATCTTTGGGCCACAGACAATCTCACCGGTTTCAAAATCTCTTCCGATTCAGTGGGTTGATGGTGTCCTCTCCAAAGGAAATAGAACCGAATTCTTTGATGATGAGTTTCGATGCCCTGTGTATGTAAAGGATGTCGTAGCAATCATACTTGCTTTGTCCAAGACATGGATATCAGAGGCTAAGCAAAGAAAATTGCTACTGAATGTTGGTGGACCGGACAGGGTATCCCGTGTTCAAATGGCTGAGACAGTTGCTGATATAAGGGGATACAACCTCTCATTAATTAAATCTGTATCTGCATCATCGGTTGATCGTGGAGTTATGTCTCCTGCTGACATATCCATGGATATAACTAAGCTAGTTCAGACGCTTGGTATTTCTCCTATTTCATTTCGAGATGGTGTCAGATTGACGCTTGAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RmlD_sub_bind

Protein Analysis

316

Amino Acids

34.51

Weight (kDa)

5.6

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 5 - 314 1.3e-39 RmlD substrate binding domain
Epimerase PF01370 6 - 229 1.1e-17 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 7 - 170 9.5e-07 GDP-mannose 4,6 dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 690
AciI CCGC 1 cut(s) 227
AclWI GGATC 3 cut(s) 278, 291, 361
AcsI RAATTY 1 cut(s) 614
AfiI CCNNNNNNNGG 1 cut(s) 690
AgeI ACCGGT 1 cut(s) 550
AgsI TTSAA 6 cut(s) 80, 184, 203, 558, 761, 944
AluBI AGCT 5 cut(s) 137, 296, 342, 470, 883
AluI AGCT 5 cut(s) 137, 296, 342, 470, 883
Alw26I GTCTC 2 cut(s) 764, 855
AlwI GGATC 3 cut(s) 278, 291, 361
AlwNI CAGNNNCTG 1 cut(s) 779
AoxI GGCC 1 cut(s) 533
ApeKI GCWGC 4 cut(s) 251, 290, 293, 467
ApoI RAATTY 1 cut(s) 614
AseI ATTAAT 1 cut(s) 806
AsiGI ACCGGT 1 cut(s) 550
Asp700I GAANNNNTTC 1 cut(s) 432
AspS9I GGNCC 3 cut(s) 224, 533, 737
AsuHPI GGTGA 3 cut(s) 100, 412, 540
AvaII GGWCC 2 cut(s) 224, 737
BamHI GGATCC 1 cut(s) 283
BarI GAAGNNNNNNTAC 2 cut(s) 395, 427
BauI CACGAG 1 cut(s) 267
BbvI GCAGC 4 cut(s) 238, 277, 305, 479
BccI CCATC 3 cut(s) 322, 578, 917
BciVI GTATCC 2 cut(s) 760, 785
BclI TGATCA 1 cut(s) 382
BcoDI GTCTC 2 cut(s) 764, 855
BfaI CTAG 2 cut(s) 156, 884
BfmI CTRYAG 1 cut(s) 291
BfuI GTATCC 2 cut(s) 760, 785
BisI GCNGC 4 cut(s) 252, 291, 294, 468
BlpI GCTNAGC 2 cut(s) 471, 705
BlsI GCNGC 4 cut(s) 253, 292, 295, 469
Bme18I GGWCC 2 cut(s) 224, 737
BmgT120I GGNCC 3 cut(s) 224, 533, 737
BmiI GGNNCC 2 cut(s) 264, 285
BmsI GCATC 3 cut(s) 153, 626, 833
BplI GAGNNNNNCTC 2 cut(s) 479, 511
Bpu1102I GCTNAGC 2 cut(s) 471, 705
BpuEI CTTGAG 1 cut(s) 103
BsaJI CCNNGG 1 cut(s) 867
BsaWI WCCGGW 2 cut(s) 550, 739
Bsc4I CCNNNNNNNGG 1 cut(s) 690
Bse118I RCCGGY 1 cut(s) 550
Bse1I ACTGG 1 cut(s) 437
Bse3DI GCAATG 1 cut(s) 199
BseDI CCNNGG 1 cut(s) 867
BseGI GGATG 1 cut(s) 661
BseLI CCNNNNNNNGG 1 cut(s) 690
BseMI GCAATG 1 cut(s) 199
BseMII CTCAG 3 cut(s) 462, 477, 759
BseNI ACTGG 1 cut(s) 437
BseRI GAGGAG 1 cut(s) 117
BseXI GCAGC 4 cut(s) 238, 277, 305, 479
BsgI GTGCAG 1 cut(s) 237
BshFI GGCC 1 cut(s) 535
BshTI ACCGGT 1 cut(s) 550
BsiSI CCGG 2 cut(s) 551, 740
BslFI GGGAC 1 cut(s) 60
BslI CCNNNNNNNGG 1 cut(s) 690
BsmAI GTCTC 2 cut(s) 764, 855
BsmFI GGGAC 1 cut(s) 60
BsmI GAATGC 1 cut(s) 104
BsnI GGCC 1 cut(s) 535
Bsp143I GATC 5 cut(s) 97, 283, 366, 382, 835
Bsp1720I GCTNAGC 2 cut(s) 471, 705
Bsp19I CCATGG 1 cut(s) 867
BspACI CCGC 1 cut(s) 227
BspANI GGCC 1 cut(s) 535
BspCNI CTCAG 3 cut(s) 463, 478, 760
BspLI GGNNCC 2 cut(s) 264, 285
BspMAI CTGCAG 1 cut(s) 295
BspPI GGATC 3 cut(s) 278, 291, 361
BsrDI GCAATG 1 cut(s) 199
BsrFI RCCGGY 1 cut(s) 550
BsrI ACTGG 1 cut(s) 437
BssAI RCCGGY 1 cut(s) 550
BssECI CCNNGG 1 cut(s) 867
BssMI GATC 5 cut(s) 97, 283, 366, 382, 835
BssSI CACGAG 1 cut(s) 267
BssT1I CCWWGG 1 cut(s) 867
Bst2BI CACGAG 1 cut(s) 267
Bst4CI ACNGT 3 cut(s) 248, 775, 948
Bst6I CTCTTC 3 cut(s) 5, 342, 570
BstAPI GCANNNNNTGC 1 cut(s) 58
BstDEI CTNAG 6 cut(s) 343, 471, 486, 705, 768, 879
BstDSI CCRYGG 1 cut(s) 867
BstF5I GGATG 1 cut(s) 661
BstKTI GATC 5 cut(s) 100, 286, 369, 385, 838
BstMAI GTCTC 2 cut(s) 764, 855
BstMBI GATC 5 cut(s) 97, 283, 366, 382, 835
BstMWI GCNNNNNNNGC 1 cut(s) 58
BstNSI RCATGY 1 cut(s) 56
BstSFI CTRYAG 1 cut(s) 291
BstV1I GCAGC 4 cut(s) 238, 277, 305, 479
BstX2I RGATCY 1 cut(s) 283
BstYI RGATCY 1 cut(s) 283
BsuI GTATCC 2 cut(s) 760, 785
BsuRI GGCC 1 cut(s) 535
BtgI CCRYGG 1 cut(s) 867
BtsCI GGATG 1 cut(s) 661
BtsIMutI CAGTG 1 cut(s) 581
CaiI CAGNNNCTG 1 cut(s) 779
Cfr10I RCCGGY 1 cut(s) 550
Cfr13I GGNCC 3 cut(s) 224, 533, 737
CseI GACGC 2 cut(s) 901, 946
CspAI ACCGGT 1 cut(s) 550
CviAII CATG 3 cut(s) 53, 690, 868
DdeI CTNAG 6 cut(s) 343, 471, 486, 705, 768, 879
DpnI GATC 5 cut(s) 99, 285, 368, 384, 837
DpnII GATC 5 cut(s) 97, 283, 366, 382, 835
Eam1104I CTCTTC 3 cut(s) 5, 342, 570
EarI CTCTTC 3 cut(s) 5, 342, 570
EciI GGCGGA 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 867
Eco32I GATATC 1 cut(s) 696
Eco47I GGWCC 2 cut(s) 224, 737
EcoRI GAATTC 1 cut(s) 614
EcoRV GATATC 1 cut(s) 696
EcoT14I CCWWGG 1 cut(s) 867
EcoT22I ATGCAT 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 867
FaeI CATG 3 cut(s) 56, 693, 871
FaqI GGGAC 1 cut(s) 60
FatI CATG 3 cut(s) 52, 689, 867
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 4 cut(s) 252, 291, 294, 468
FokI GGATG 1 cut(s) 668
Fsp4HI GCNGC 4 cut(s) 252, 291, 294, 468
FspBI CTAG 2 cut(s) 156, 884
GluI GCNGC 4 cut(s) 252, 291, 294, 468
HaeIII GGCC 1 cut(s) 535
HapII CCGG 2 cut(s) 551, 740
HgaI GACGC 2 cut(s) 901, 946
Hin1II CATG 3 cut(s) 56, 693, 871
HincII GTYRAC 1 cut(s) 378
HindII GTYRAC 1 cut(s) 378
HindIII AAGCTT 1 cut(s) 135
HinfI GANTC 2 cut(s) 76, 571
HpaII CCGG 2 cut(s) 551, 740
HphI GGTGA 3 cut(s) 100, 412, 540
Hpy166II GTNNAC 3 cut(s) 244, 378, 737
Hpy188I TCNGA 6 cut(s) 73, 366, 570, 700, 891, 932
Hpy188III TCNNGA 2 cut(s) 80, 920
Hpy8I GTNNAC 3 cut(s) 244, 378, 737
HpyAV CCTTC 1 cut(s) 389
HpyCH4III ACNGT 3 cut(s) 248, 775, 948
HpyCH4V TGCA 6 cut(s) 61, 149, 254, 293, 506, 824
HpyF10VI GCNNNNNNNGC 1 cut(s) 58
HpyF3I CTNAG 6 cut(s) 343, 471, 486, 705, 768, 879
Hsp92II CATG 3 cut(s) 56, 693, 871
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 5 cut(s) 97, 283, 366, 382, 835
Lsp1109I GCAGC 4 cut(s) 238, 277, 305, 479
LweI GCATC 3 cut(s) 153, 626, 833
MaeI CTAG 2 cut(s) 156, 884
MaeIII GTNAC 1 cut(s) 38
MalI GATC 5 cut(s) 99, 285, 368, 384, 837
MboI GATC 5 cut(s) 97, 283, 366, 382, 835
MboII GAAGA 6 cut(s) 22, 309, 353, 359, 431, 557
MflI RGATCY 1 cut(s) 283
MluCI AATT 6 cut(s) 328, 355, 507, 614, 716, 807
MmeI TCCRAC 1 cut(s) 7
MnlI CCTC 7 cut(s) 23, 135, 138, 276, 602, 694, 809
Mph1103I ATGCAT 1 cut(s) 151
MroXI GAANNNNTTC 1 cut(s) 432
MseI TTAA 4 cut(s) 306, 327, 806, 810
MslI CAYNNNNRTG 1 cut(s) 866
MspA1I CMGCKG 1 cut(s) 470
MspI CCGG 2 cut(s) 551, 740
Mva1269I GAATGC 1 cut(s) 104
MwoI GCNNNNNNNGC 1 cut(s) 58
NcoI CCATGG 1 cut(s) 867
NdeII GATC 5 cut(s) 97, 283, 366, 382, 835
NlaIII CATG 3 cut(s) 56, 693, 871
NlaIV GGNNCC 2 cut(s) 264, 285
NsiI ATGCAT 1 cut(s) 151
NspI RCATGY 1 cut(s) 56
PacI TTAATTAA 1 cut(s) 810
PctI GAATGC 1 cut(s) 104
PdmI GAANNNNTTC 1 cut(s) 432
PfeI GAWTC 2 cut(s) 76, 571
PflMI CCANNNNNTGG 1 cut(s) 690
PinAI ACCGGT 1 cut(s) 550
PkrI GCNGC 4 cut(s) 253, 292, 295, 469
PshBI ATTAAT 1 cut(s) 806
PspN4I GGNNCC 2 cut(s) 264, 285
PspPI GGNCC 3 cut(s) 224, 533, 737
PsrI GAACNNNNNNTAC 2 cut(s) 741, 773
PstI CTGCAG 1 cut(s) 295
PstNI CAGNNNCTG 1 cut(s) 779
PsuI RGATCY 1 cut(s) 283
PvuII CAGCTG 1 cut(s) 470
RseI CAYNNNNRTG 1 cut(s) 866
SaqAI TTAA 4 cut(s) 306, 327, 806, 810
SatI GCNGC 4 cut(s) 252, 291, 294, 468
Sau3AI GATC 5 cut(s) 97, 283, 366, 382, 835
Sau96I GGNCC 3 cut(s) 224, 533, 737
SetI ASST 7 cut(s) 40, 139, 298, 344, 472, 801, 885
SfaNI GCATC 3 cut(s) 153, 626, 833
SfcI CTRYAG 1 cut(s) 291
SinI GGWCC 2 cut(s) 224, 737
SmiMI CAYNNNNRTG 1 cut(s) 866
SmlI CTYRAG 1 cut(s) 118
SmoI CTYRAG 1 cut(s) 118
Sse9I AATT 6 cut(s) 328, 355, 507, 614, 716, 807
SsiI CCGC 1 cut(s) 227
SspMI CTAG 2 cut(s) 156, 884
StyI CCWWGG 1 cut(s) 867
TaaI ACNGT 3 cut(s) 248, 775, 948
TaqI TCGA 3 cut(s) 338, 634, 919
TasI AATT 6 cut(s) 328, 355, 507, 614, 716, 807
TfiI GAWTC 2 cut(s) 76, 571
Tru1I TTAA 4 cut(s) 306, 327, 806, 810
Tru9I TTAA 4 cut(s) 306, 327, 806, 810
TscAI CASTG 1 cut(s) 581
TseI GCWGC 4 cut(s) 251, 290, 293, 467
TspDTI ATGAA 4 cut(s) 408, 441, 469, 903
TspRI CASTG 1 cut(s) 581
Van91I CCANNNNNTGG 1 cut(s) 690
VpaK11BI GGWCC 2 cut(s) 224, 737
VspI ATTAAT 1 cut(s) 806
XapI RAATTY 1 cut(s) 614
XceI RCATGY 1 cut(s) 56
XmnI GAANNNNTTC 1 cut(s) 432
XspI CTAG 2 cut(s) 156, 884
Zsp2I ATGCAT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.