Rmu_sc0001207.1_g000042

Methionine adenosyltransferase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001207.1
OG0001020
Physical Location & Seq
Reverse (-)
148866 .. 151795
2930 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001207.1_g000042.1.cds

Sequence Viewer

Length: 1014 bp
atggctctctttagcaaccctagtggctattctgtcactctagccgccgacactgtgctagctcctgtccggctacgccccggcaatggcgacggcctttggccttgccaatgcacgttgggtgtggccggacggtcgagccgtggttgcagattgggtcggagtggactcggggctctgctggggagtagcggctttgggttgccggcttggttatggcgtagagttcagggtgcggcctggtgtgggatgatgattggtggtgtttctcgctggtggtccggcagtgacgacgtctttctgtccgacatcagtggaggagacggggtggaggcgcggcaaggctggtctagagtcgggaagggttggggacggccattttctggatttgctttctctgggctttggcaatggctgtgggcctattatgggctgaagttgggttggggttacttgggacagcatgcattgcaaggcttttcagagattcaagaaaccactcctcgtgatctggcatttacccaccactcaagtcctcctccccaagctttgctcaatgcatttcctagtgtgatgcctttctctgtggatttgaagtctggcaatggatttgaagccatctctcgtcagtttggtccgcctgatgtggtagtaaactgtgctgcactttcggttcctcgtgcctgtgaaatggatcctgctgcagctatgtcagttaatgtgccatcttctcttgttaattggttatcgagcttagaagagagtaattatcttctgatccatttgtcaactgatcaagtttatgaaggggtgaagtccttttacaaggaagatgatgaagttgttccagtaaatgtttatgggaaatcaaaagtggcagctgagcagttcattaccgagaaatgctcaaactttgcaattttgagaagcagtatcatctttgggccacagacaatctcaccggtttcaaaatctcttccgattcaggtttgcaaaagagaaaacagacattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RmlD_sub_bind

Protein Analysis

337

Amino Acids

36.49

Weight (kDa)

8.1

Isoelectric Point (pI)

50.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 297
AccB7I CCANNNNNTGG 1 cut(s) 383
AccII CGCG 1 cut(s) 337
AciI CCGC 5 cut(s) 45, 192, 236, 337, 638
AclWI GGATC 3 cut(s) 689, 702, 772
AcoI YGGCCR 2 cut(s) 126, 374
AcuI CTGAAG 1 cut(s) 455
AcyI GRCGYC 1 cut(s) 294
AfiI CCNNNNNNNGG 4 cut(s) 86, 246, 383, 429
AgeI ACCGGT 1 cut(s) 961
AgsI TTSAA 4 cut(s) 491, 595, 614, 969
AjnI CCWGG 1 cut(s) 239
AluBI AGCT 5 cut(s) 62, 548, 707, 753, 881
AluI AGCT 5 cut(s) 62, 548, 707, 753, 881
Alw26I GTCTC 1 cut(s) 315
AlwI GGATC 3 cut(s) 689, 702, 772
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 7 cut(s) 94, 101, 126, 237, 374, 420, 944
ApeKI GCWGC 4 cut(s) 662, 701, 704, 878
AsiGI ACCGGT 1 cut(s) 961
Asp700I GAANNNNTTC 1 cut(s) 843
AspLEI GCGC 1 cut(s) 337
AspS9I GGNCC 4 cut(s) 279, 420, 635, 944
AsuC2I CCSGG 1 cut(s) 81
AsuHPI GGTGA 2 cut(s) 823, 951
AsuNHI GCTAGC 1 cut(s) 58
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 2 cut(s) 279, 635
BamHI GGATCC 1 cut(s) 694
BanII GRGCYC 1 cut(s) 178
BarI GAAGNNNNNNTAC 2 cut(s) 806, 838
BauI CACGAG 2 cut(s) 504, 678
BbvI GCAGC 4 cut(s) 649, 688, 716, 890
BccI CCATC 2 cut(s) 626, 733
BceAI ACGGC 3 cut(s) 109, 126, 389
BciT130I CCWGG 1 cut(s) 241
BclI TGATCA 1 cut(s) 793
BcnI CCSGG 1 cut(s) 81
BcoDI GTCTC 1 cut(s) 315
BfaI CTAG 5 cut(s) 21, 41, 59, 351, 567
BfmI CTRYAG 1 cut(s) 702
BisI GCNGC 8 cut(s) 45, 193, 237, 338, 663, 702, 705, 879
BlpI GCTNAGC 1 cut(s) 882
BlsI GCNGC 8 cut(s) 46, 194, 238, 339, 664, 703, 706, 880
Bme1390I CCNGG 2 cut(s) 81, 241
Bme18I GGWCC 2 cut(s) 279, 635
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 4 cut(s) 279, 420, 635, 944
BmiI GGNNCC 2 cut(s) 675, 696
BmrFI CCNGG 2 cut(s) 81, 241
BmsI GCATC 1 cut(s) 564
BmtI GCTAGC 1 cut(s) 62
BplI GAGNNNNNCTC 2 cut(s) 890, 922
Bpu1102I GCTNAGC 1 cut(s) 882
BpuEI CTTGAG 1 cut(s) 514
BpuMI CCSGG 1 cut(s) 81
BsaHI GRCGYC 1 cut(s) 294
BsaJI CCNNGG 2 cut(s) 79, 142
BsaWI WCCGGW 1 cut(s) 961
BsaXI ACNNNNNCTCC 2 cut(s) 312, 342
Bsc4I CCNNNNNNNGG 4 cut(s) 86, 246, 383, 429
Bse118I RCCGGY 2 cut(s) 205, 961
Bse1I ACTGG 1 cut(s) 848
Bse3DI GCAATG 4 cut(s) 91, 416, 467, 610
BseBI CCWGG 1 cut(s) 241
BseDI CCNNGG 2 cut(s) 79, 142
BseGI GGATG 1 cut(s) 255
BseLI CCNNNNNNNGG 4 cut(s) 86, 246, 383, 429
BseMI GCAATG 4 cut(s) 91, 416, 467, 610
BseMII CTCAG 1 cut(s) 873
BseNI ACTGG 1 cut(s) 848
BseRI GAGGAG 3 cut(s) 333, 492, 528
BseXI GCAGC 4 cut(s) 649, 688, 716, 890
BseYI CCCAGC 1 cut(s) 181
BsgI GTGCAG 1 cut(s) 648
Bsh1236I CGCG 1 cut(s) 337
Bsh1285I CGRYCG 1 cut(s) 137
BshFI GGCC 7 cut(s) 96, 103, 128, 239, 376, 422, 946
BshTI ACCGGT 1 cut(s) 961
BsiEI CGRYCG 1 cut(s) 137
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 6 cut(s) 70, 81, 129, 206, 282, 962
BslFI GGGAC 2 cut(s) 384, 471
BslI CCNNNNNNNGG 4 cut(s) 86, 246, 383, 429
BsmAI GTCTC 1 cut(s) 315
BsmBI CGTCTC 1 cut(s) 315
BsmFI GGGAC 2 cut(s) 384, 471
BsnI GGCC 7 cut(s) 96, 103, 128, 239, 376, 422, 946
BsoBI CYCGRG 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 178
Bsp143I GATC 4 cut(s) 508, 694, 777, 793
Bsp1720I GCTNAGC 1 cut(s) 882
BspACI CCGC 5 cut(s) 45, 192, 236, 337, 638
BspANI GGCC 7 cut(s) 96, 103, 128, 239, 376, 422, 946
BspCNI CTCAG 1 cut(s) 874
BspFNI CGCG 1 cut(s) 337
BspLI GGNNCC 2 cut(s) 675, 696
BspMAI CTGCAG 1 cut(s) 706
BspOI GCTAGC 1 cut(s) 62
BspPI GGATC 3 cut(s) 689, 702, 772
BsrDI GCAATG 4 cut(s) 91, 416, 467, 610
BsrFI RCCGGY 2 cut(s) 205, 961
BsrI ACTGG 1 cut(s) 848
BssAI RCCGGY 2 cut(s) 205, 961
BssECI CCNNGG 2 cut(s) 79, 142
BssMI GATC 4 cut(s) 508, 694, 777, 793
BssNI GRCGYC 1 cut(s) 294
BssSI CACGAG 2 cut(s) 504, 678
Bst2BI CACGAG 2 cut(s) 504, 678
Bst2UI CCWGG 1 cut(s) 241
Bst4CI ACNGT 3 cut(s) 55, 135, 659
Bst6I CTCTTC 2 cut(s) 753, 981
BstACI GRCGYC 1 cut(s) 294
BstAPI GCANNNNNTGC 1 cut(s) 469
BstC8I GCNNGC 3 cut(s) 60, 207, 465
BstDEI CTNAG 2 cut(s) 754, 882
BstDSI CCRYGG 1 cut(s) 142
BstF5I GGATG 1 cut(s) 255
BstFNI CGCG 1 cut(s) 337
BstHHI GCGC 1 cut(s) 337
BstKTI GATC 4 cut(s) 511, 697, 780, 796
BstMAI GTCTC 1 cut(s) 315
BstMBI GATC 4 cut(s) 508, 694, 777, 793
BstMCI CGRYCG 1 cut(s) 137
BstMWI GCNNNNNNNGC 2 cut(s) 147, 469
BstNI CCWGG 1 cut(s) 241
BstNSI RCATGY 1 cut(s) 467
BstSCI CCNGG 2 cut(s) 79, 239
BstSFI CTRYAG 1 cut(s) 702
BstUI CGCG 1 cut(s) 337
BstV1I GCAGC 4 cut(s) 649, 688, 716, 890
BstX2I RGATCY 1 cut(s) 694
BstYI RGATCY 1 cut(s) 694
BsuRI GGCC 7 cut(s) 96, 103, 128, 239, 376, 422, 946
BtgI CCRYGG 1 cut(s) 142
BtsCI GGATG 1 cut(s) 255
BtsI GCAGTG 1 cut(s) 292
BtsIMutI CAGTG 3 cut(s) 51, 292, 319
Cac8I GCNNGC 3 cut(s) 60, 207, 465
CfoI GCGC 1 cut(s) 337
Cfr10I RCCGGY 2 cut(s) 205, 961
Cfr13I GGNCC 4 cut(s) 279, 420, 635, 944
CspAI ACCGGT 1 cut(s) 961
CspCI CAANNNNNGTGG 4 cut(s) 4, 39, 398, 433
CviAII CATG 1 cut(s) 464
DdeI CTNAG 2 cut(s) 754, 882
DpnI GATC 4 cut(s) 510, 696, 779, 795
DpnII GATC 4 cut(s) 508, 694, 777, 793
EaeI YGGCCR 2 cut(s) 126, 374
Eam1104I CTCTTC 2 cut(s) 753, 981
EarI CTCTTC 2 cut(s) 753, 981
EciI GGCGGA 1 cut(s) 627
Eco24I GRGCYC 1 cut(s) 178
Eco47I GGWCC 2 cut(s) 279, 635
Eco57I CTGAAG 1 cut(s) 455
Eco88I CYCGRG 1 cut(s) 170
EcoRII CCWGG 1 cut(s) 239
EcoT22I ATGCAT 2 cut(s) 469, 562
EcoT38I GRGCYC 1 cut(s) 178
Esp3I CGTCTC 1 cut(s) 315
FaeI CATG 1 cut(s) 467
FaiI YATR 6 cut(s) 217, 429, 465, 710, 804, 861
FaqI GGGAC 2 cut(s) 384, 471
FatI CATG 1 cut(s) 463
FbaI TGATCA 1 cut(s) 793
Fnu4HI GCNGC 8 cut(s) 45, 193, 237, 338, 663, 702, 705, 879
FokI GGATG 1 cut(s) 262
FriOI GRGCYC 1 cut(s) 178
Fsp4HI GCNGC 8 cut(s) 45, 193, 237, 338, 663, 702, 705, 879
FspBI CTAG 5 cut(s) 21, 41, 59, 351, 567
GlaI GCGC 1 cut(s) 336
GluI GCNGC 8 cut(s) 45, 193, 237, 338, 663, 702, 705, 879
GsaI CCCAGC 1 cut(s) 185
HaeIII GGCC 7 cut(s) 96, 103, 128, 239, 376, 422, 946
HapII CCGG 6 cut(s) 70, 81, 129, 206, 282, 962
HhaI GCGC 1 cut(s) 337
Hin1I GRCGYC 1 cut(s) 294
Hin1II CATG 1 cut(s) 467
Hin6I GCGC 1 cut(s) 335
HinP1I GCGC 1 cut(s) 335
HincII GTYRAC 1 cut(s) 789
HindII GTYRAC 1 cut(s) 789
HindIII AAGCTT 1 cut(s) 546
HinfI GANTC 4 cut(s) 168, 354, 487, 982
HpaII CCGG 6 cut(s) 70, 81, 129, 206, 282, 962
HphI GGTGA 2 cut(s) 823, 951
Hpy166II GTNNAC 3 cut(s) 167, 655, 789
Hpy188I TCNGA 5 cut(s) 162, 307, 484, 777, 981
Hpy188III TCNNGA 5 cut(s) 351, 358, 384, 491, 506
Hpy8I GTNNAC 3 cut(s) 167, 655, 789
Hpy99I CGWCG 2 cut(s) 95, 296
HpyAV CCTTC 2 cut(s) 355, 800
HpyCH4III ACNGT 3 cut(s) 55, 135, 659
HpyCH4IV ACGT 2 cut(s) 116, 294
HpyCH4V TGCA 9 cut(s) 114, 150, 467, 472, 560, 665, 704, 917, 993
HpyF10VI GCNNNNNNNGC 2 cut(s) 147, 469
HpyF3I CTNAG 2 cut(s) 754, 882
HpySE526I ACGT 2 cut(s) 116, 294
Hsp92I GRCGYC 1 cut(s) 294
Hsp92II CATG 1 cut(s) 467
HspAI GCGC 1 cut(s) 335
KroI GCCGGC 1 cut(s) 205
KroNI GCCGGC 1 cut(s) 207
Ksp22I TGATCA 1 cut(s) 793
Kzo9I GATC 4 cut(s) 508, 694, 777, 793
LmnI GCTCC 1 cut(s) 67
Lsp1109I GCAGC 4 cut(s) 649, 688, 716, 890
LweI GCATC 1 cut(s) 564
MaeI CTAG 5 cut(s) 21, 41, 59, 351, 567
MaeII ACGT 2 cut(s) 116, 294
MaeIII GTNAC 3 cut(s) 34, 287, 449
MalI GATC 4 cut(s) 510, 696, 779, 795
MboI GATC 4 cut(s) 508, 694, 777, 793
MboII GAAGA 5 cut(s) 720, 764, 770, 842, 968
MflI RGATCY 1 cut(s) 694
MhlI GDGCHC 1 cut(s) 178
MluCI AATT 3 cut(s) 739, 766, 918
MlyI GAGTC 2 cut(s) 162, 363
MmeI TCCRAC 2 cut(s) 140, 330
MnlI CCTC 6 cut(s) 311, 325, 513, 546, 549, 687
Mph1103I ATGCAT 2 cut(s) 469, 562
MroNI GCCGGC 1 cut(s) 205
MroXI GAANNNNTTC 1 cut(s) 843
MseI TTAA 3 cut(s) 717, 738, 1012
MspA1I CMGCKG 1 cut(s) 881
MspI CCGG 6 cut(s) 70, 81, 129, 206, 282, 962
MspR9I CCNGG 2 cut(s) 81, 241
MvaI CCWGG 1 cut(s) 241
MvnI CGCG 1 cut(s) 337
MwoI GCNNNNNNNGC 2 cut(s) 147, 469
NaeI GCCGGC 1 cut(s) 207
NciI CCSGG 1 cut(s) 81
NdeII GATC 4 cut(s) 508, 694, 777, 793
NgoMIV GCCGGC 1 cut(s) 205
NheI GCTAGC 1 cut(s) 58
NlaIII CATG 1 cut(s) 467
NlaIV GGNNCC 2 cut(s) 675, 696
NmuCI GTSAC 2 cut(s) 34, 287
NsiI ATGCAT 2 cut(s) 469, 562
NspI RCATGY 1 cut(s) 467
PaeI GCATGC 1 cut(s) 467
PcsI WCGNNNNNNNCGW 1 cut(s) 139
PdiI GCCGGC 1 cut(s) 207
PdmI GAANNNNTTC 1 cut(s) 843
PfeI GAWTC 2 cut(s) 487, 982
PflFI GACNNNGTC 1 cut(s) 293
PflMI CCANNNNNTGG 1 cut(s) 383
PinAI ACCGGT 1 cut(s) 961
PkrI GCNGC 8 cut(s) 46, 194, 238, 339, 664, 703, 706, 880
PleI GAGTC 2 cut(s) 162, 362
PpsI GAGTC 2 cut(s) 162, 362
Psp6I CCWGG 1 cut(s) 239
PspFI CCCAGC 1 cut(s) 181
PspGI CCWGG 1 cut(s) 239
PspN4I GGNNCC 2 cut(s) 675, 696
PspPI GGNCC 4 cut(s) 279, 420, 635, 944
PstI CTGCAG 1 cut(s) 706
PsuI RGATCY 1 cut(s) 694
PsyI GACNNNGTC 1 cut(s) 293
PvuII CAGCTG 1 cut(s) 881
SaqAI TTAA 3 cut(s) 717, 738, 1012
SatI GCNGC 8 cut(s) 45, 193, 237, 338, 663, 702, 705, 879
Sau3AI GATC 4 cut(s) 508, 694, 777, 793
Sau96I GGNCC 4 cut(s) 279, 420, 635, 944
SchI GAGTC 2 cut(s) 162, 363
ScrFI CCNGG 2 cut(s) 81, 241
SduI GDGCHC 1 cut(s) 178
SetI ASST 8 cut(s) 64, 119, 297, 550, 709, 755, 883, 990
SfaNI GCATC 1 cut(s) 564
SfcI CTRYAG 1 cut(s) 702
SinI GGWCC 2 cut(s) 279, 635
SmlI CTYRAG 1 cut(s) 529
SmoI CTYRAG 1 cut(s) 529
SphI GCATGC 1 cut(s) 467
Sse9I AATT 3 cut(s) 739, 766, 918
SsiI CCGC 5 cut(s) 45, 192, 236, 337, 638
SspMI CTAG 5 cut(s) 21, 41, 59, 351, 567
StyD4I CCNGG 2 cut(s) 79, 239
TaaI ACNGT 3 cut(s) 55, 135, 659
TaiI ACGT 2 cut(s) 119, 297
TaqI TCGA 2 cut(s) 137, 749
TasI AATT 3 cut(s) 739, 766, 918
TauI GCSGC 4 cut(s) 47, 195, 239, 340
TfiI GAWTC 2 cut(s) 487, 982
Tru1I TTAA 3 cut(s) 717, 738, 1012
Tru9I TTAA 3 cut(s) 717, 738, 1012
TscAI CASTG 3 cut(s) 58, 292, 319
TseFI GTSAC 2 cut(s) 34, 287
TseI GCWGC 4 cut(s) 662, 701, 704, 878
Tsp45I GTSAC 2 cut(s) 34, 287
TspDTI ATGAA 3 cut(s) 819, 852, 880
TspRI CASTG 3 cut(s) 58, 292, 319
Tth111I GACNNNGTC 1 cut(s) 293
Van91I CCANNNNNTGG 1 cut(s) 383
VpaK11BI GGWCC 2 cut(s) 279, 635
XbaI TCTAGA 1 cut(s) 350
XceI RCATGY 1 cut(s) 467
XmnI GAANNNNTTC 1 cut(s) 843
XspI CTAG 5 cut(s) 21, 41, 59, 351, 567
ZraI GACGTC 1 cut(s) 295
Zsp2I ATGCAT 2 cut(s) 469, 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.