Rroxscaffold_4G00301960

Methionine adenosyltransferase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001020
Physical Location & Seq
Reverse (-)
22330390 .. 22333369
2980 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00301960.1

Sequence Viewer

Length: 576 bp
ATGGGGACTGATTTGAATTCCCATCAGTTTATGTTTTACAAGGAAGATGATGAAGTTGTTCCAGTAAATGTTTATGGGAAATCAAAAGTGGCAGCTGAGCAGTTCATTACTGAGAAATGCTCAAACTTTGCAATTTTGAGAAGCAGTATCATCTTTGGGCCACAGACAATCTCACCGGTTTCAAAATCTCTTCTGATTCAGTGGGTTGATGGTGTCCTCTCCAAAGGAAATACAACCGAATTCTTTCATGATGAGTTTCGATGCCCTGTGTATGTAAAGGATGTTGTAGCAATCATACTTGCTTTGTCCAAGACATGGATATCAGAGGCTAAGCAAAGAAAATTGCTACTGAATGTTGGTGGACCGGACAGGGTATCCCGTGTACAAATGGCTAAGACGGTTGCTGATATAAGGGGATACAACCTCTCATTAATTAAATCTGTATCTGCATCATCGGTTGATCGTGGAGTTATGTCTCCTGCTGACATATCCATGGATATAACTAAGCTAGTTCAGACGCTCGGTATTTCTCCTATTTCATTTCGAGATGGTGTCAGATTGACGCTTGAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RmlD_sub_bind

Protein Analysis

191

Amino Acids

21.13

Weight (kDa)

8.52

Isoelectric Point (pI)

29.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 11 - 189 1.7e-23 RmlD substrate binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 315
AcsI RAATTY 2 cut(s) 16, 239
AfaI GTAC 1 cut(s) 384
AfiI CCNNNNNNNGG 1 cut(s) 315
AgeI ACCGGT 1 cut(s) 175
AgsI TTSAA 3 cut(s) 16, 183, 569
AluBI AGCT 2 cut(s) 95, 508
AluI AGCT 2 cut(s) 95, 508
Alw26I GTCTC 1 cut(s) 480
AoxI GGCC 1 cut(s) 158
ApeKI GCWGC 1 cut(s) 92
ApoI RAATTY 2 cut(s) 16, 239
AseI ATTAAT 1 cut(s) 431
AsiGI ACCGGT 1 cut(s) 175
Asp700I GAANNNNTTC 2 cut(s) 57, 243
AspS9I GGNCC 2 cut(s) 158, 362
AsuHPI GGTGA 1 cut(s) 165
AvaII GGWCC 1 cut(s) 362
BbvI GCAGC 1 cut(s) 104
BccI CCATC 3 cut(s) 30, 203, 542
BciVI GTATCC 2 cut(s) 385, 410
BcoDI GTCTC 1 cut(s) 480
BfaI CTAG 1 cut(s) 509
BfuI GTATCC 2 cut(s) 385, 410
BisI GCNGC 1 cut(s) 93
BlpI GCTNAGC 2 cut(s) 96, 330
BlsI GCNGC 1 cut(s) 94
Bme18I GGWCC 1 cut(s) 362
BmgT120I GGNCC 2 cut(s) 158, 362
BmsI GCATC 2 cut(s) 251, 458
BplI GAGNNNNNCTC 2 cut(s) 104, 136
Bpu1102I GCTNAGC 2 cut(s) 96, 330
BsaJI CCNNGG 1 cut(s) 492
BsaWI WCCGGW 2 cut(s) 175, 364
Bsc4I CCNNNNNNNGG 1 cut(s) 315
Bse118I RCCGGY 1 cut(s) 175
Bse1I ACTGG 1 cut(s) 62
BseDI CCNNGG 1 cut(s) 492
BseGI GGATG 1 cut(s) 286
BseLI CCNNNNNNNGG 1 cut(s) 315
BseMII CTCAG 2 cut(s) 87, 102
BseNI ACTGG 1 cut(s) 62
BseXI GCAGC 1 cut(s) 104
BshFI GGCC 1 cut(s) 160
BshTI ACCGGT 1 cut(s) 175
BsiSI CCGG 2 cut(s) 176, 365
BslFI GGGAC 1 cut(s) 19
BslI CCNNNNNNNGG 1 cut(s) 315
BsmAI GTCTC 1 cut(s) 480
BsmFI GGGAC 1 cut(s) 19
BsnI GGCC 1 cut(s) 160
Bsp1407I TGTACA 1 cut(s) 382
Bsp143I GATC 1 cut(s) 460
Bsp1720I GCTNAGC 2 cut(s) 96, 330
Bsp19I CCATGG 1 cut(s) 492
BspANI GGCC 1 cut(s) 160
BspCNI CTCAG 2 cut(s) 88, 103
BspHI TCATGA 1 cut(s) 247
BsrFI RCCGGY 1 cut(s) 175
BsrGI TGTACA 1 cut(s) 382
BsrI ACTGG 1 cut(s) 62
BssAI RCCGGY 1 cut(s) 175
BssECI CCNNGG 1 cut(s) 492
BssMI GATC 1 cut(s) 460
BssT1I CCWWGG 1 cut(s) 492
Bst4CI ACNGT 2 cut(s) 400, 573
Bst6I CTCTTC 1 cut(s) 195
BstAUI TGTACA 1 cut(s) 382
BstDEI CTNAG 5 cut(s) 96, 111, 330, 393, 504
BstDSI CCRYGG 1 cut(s) 492
BstF5I GGATG 1 cut(s) 286
BstKTI GATC 1 cut(s) 463
BstMAI GTCTC 1 cut(s) 480
BstMBI GATC 1 cut(s) 460
BstV1I GCAGC 1 cut(s) 104
BsuI GTATCC 2 cut(s) 385, 410
BsuRI GGCC 1 cut(s) 160
BtgI CCRYGG 1 cut(s) 492
BtsCI GGATG 1 cut(s) 286
BtsIMutI CAGTG 1 cut(s) 206
CciI TCATGA 1 cut(s) 247
Cfr10I RCCGGY 1 cut(s) 175
Cfr13I GGNCC 2 cut(s) 158, 362
CseI GACGC 2 cut(s) 526, 571
Csp6I GTAC 1 cut(s) 383
CspAI ACCGGT 1 cut(s) 175
CviAII CATG 3 cut(s) 248, 315, 493
CviJI RGCY 5 cut(s) 95, 160, 329, 392, 508
CviKI_1 RGCY 5 cut(s) 95, 160, 329, 392, 508
CviQI GTAC 1 cut(s) 383
DdeI CTNAG 5 cut(s) 96, 111, 330, 393, 504
DpnI GATC 1 cut(s) 462
DpnII GATC 1 cut(s) 460
Eam1104I CTCTTC 1 cut(s) 195
EarI CTCTTC 1 cut(s) 195
Eco130I CCWWGG 1 cut(s) 492
Eco32I GATATC 1 cut(s) 321
Eco47I GGWCC 1 cut(s) 362
EcoRI GAATTC 2 cut(s) 16, 239
EcoRV GATATC 1 cut(s) 321
EcoT14I CCWWGG 1 cut(s) 492
ErhI CCWWGG 1 cut(s) 492
FaeI CATG 3 cut(s) 251, 318, 496
FaqI GGGAC 1 cut(s) 19
FatI CATG 3 cut(s) 247, 314, 492
Fnu4HI GCNGC 1 cut(s) 93
FokI GGATG 1 cut(s) 293
Fsp4HI GCNGC 1 cut(s) 93
FspBI CTAG 1 cut(s) 509
GluI GCNGC 1 cut(s) 93
HaeIII GGCC 1 cut(s) 160
HapII CCGG 2 cut(s) 176, 365
HgaI GACGC 2 cut(s) 526, 571
Hin1II CATG 3 cut(s) 251, 318, 496
HinfI GANTC 1 cut(s) 196
HpaII CCGG 2 cut(s) 176, 365
HphI GGTGA 1 cut(s) 165
Hpy166II GTNNAC 2 cut(s) 362, 383
Hpy188I TCNGA 4 cut(s) 195, 325, 516, 557
Hpy188III TCNNGA 2 cut(s) 248, 545
Hpy8I GTNNAC 2 cut(s) 362, 383
HpyCH4III ACNGT 2 cut(s) 400, 573
HpyCH4V TGCA 2 cut(s) 131, 449
HpyF3I CTNAG 5 cut(s) 96, 111, 330, 393, 504
Hsp92II CATG 3 cut(s) 251, 318, 496
Kzo9I GATC 1 cut(s) 460
LpnPI CCDG 6 cut(s) 75, 189, 279, 355, 378, 492
Lsp1109I GCAGC 1 cut(s) 104
LweI GCATC 2 cut(s) 251, 458
MaeI CTAG 1 cut(s) 509
MalI GATC 1 cut(s) 462
MboI GATC 1 cut(s) 460
MboII GAAGA 2 cut(s) 56, 182
MluCI AATT 5 cut(s) 16, 132, 239, 341, 432
MnlI CCTC 3 cut(s) 227, 319, 434
MroXI GAANNNNTTC 2 cut(s) 57, 243
MseI TTAA 2 cut(s) 431, 435
MslI CAYNNNNRTG 1 cut(s) 491
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 2 cut(s) 176, 365
NcoI CCATGG 1 cut(s) 492
NdeII GATC 1 cut(s) 460
NlaIII CATG 3 cut(s) 251, 318, 496
PacI TTAATTAA 1 cut(s) 435
PagI TCATGA 1 cut(s) 247
PdmI GAANNNNTTC 2 cut(s) 57, 243
PfeI GAWTC 1 cut(s) 196
PflMI CCANNNNNTGG 1 cut(s) 315
PinAI ACCGGT 1 cut(s) 175
PkrI GCNGC 1 cut(s) 94
PshBI ATTAAT 1 cut(s) 431
PspPI GGNCC 2 cut(s) 158, 362
PvuII CAGCTG 1 cut(s) 95
RsaI GTAC 1 cut(s) 384
RsaNI GTAC 1 cut(s) 383
RseI CAYNNNNRTG 1 cut(s) 491
SaqAI TTAA 2 cut(s) 431, 435
SatI GCNGC 1 cut(s) 93
Sau3AI GATC 1 cut(s) 460
Sau96I GGNCC 2 cut(s) 158, 362
SetI ASST 3 cut(s) 97, 426, 510
SfaNI GCATC 2 cut(s) 251, 458
SinI GGWCC 1 cut(s) 362
SmiMI CAYNNNNRTG 1 cut(s) 491
Sse9I AATT 5 cut(s) 16, 132, 239, 341, 432
SspMI CTAG 1 cut(s) 509
StyI CCWWGG 1 cut(s) 492
TaaI ACNGT 2 cut(s) 400, 573
TaqI TCGA 2 cut(s) 259, 544
TasI AATT 5 cut(s) 16, 132, 239, 341, 432
TatI WGTACW 1 cut(s) 382
TfiI GAWTC 1 cut(s) 196
Tru1I TTAA 2 cut(s) 431, 435
Tru9I TTAA 2 cut(s) 431, 435
TscAI CASTG 1 cut(s) 206
TseI GCWGC 1 cut(s) 92
TspDTI ATGAA 4 cut(s) 66, 94, 236, 528
TspRI CASTG 1 cut(s) 206
Van91I CCANNNNNTGG 1 cut(s) 315
VpaK11BI GGWCC 1 cut(s) 362
VspI ATTAAT 1 cut(s) 431
XapI RAATTY 2 cut(s) 16, 239
XmnI GAANNNNTTC 2 cut(s) 57, 243
XspI CTAG 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.