RCHIOBHM_CHR0C39G0503341

Methionine adenosyltransferase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_chinensis
Unknown
OG0001020
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 957 bp
ATGAGTAGGAAGAGAGTTTTGATAGTTGGAGGAACAGGTTACTTGGGACAGCATGTATTGCAAGGCTTTTCAGAGATTCAAGAAACCACTCCTTGTGATCTGGCATTTACCCACCACTCAAGTCCTCCTCCCCAAGCTTTGCTCAATGCATTTCCTAGTGTGCTGCCTTTCTCTGTGGATTTGAAGTCTGGCAATGGATTTGAAGCCATTTCTCATCAGTTTGGTCCGCCTGATGTGGTAGTAAACTGTGCTGCACTTTCGGTTCCTCGTGCCTGTGAAATGGATCCTGCTGCAGCTATGTCAGTTAATGTGCCATCTTCTCTTGTTAATTGGTTATCGAGCTTAGAAGAGAGTAATTATCTTCTGATCCATTTGTCAACTGATCAAGACCCCTGTTGGTCTACAGTTCAGTCCTTTTACAAGGAAGATGATGAAGTTGTTCCAGTAAATGTTTATGGGAAATCAAAAGTGGCAGCTGAGCAGTTCATTACTGAGAAATGCTCAAACTTTGCAATTTTGAGAAGCAGTATCATCTTTGGGCCACAGACAATCTCACCGGTTTCAAAATCTCTTCCGATTCAGTGGGTTGATGGTGTCCTCTCCAAAGGAAATACAACTGAATTCTTTCATGATGAGTTTCGGTGCCCTGTGTATGTAAAGGATGTCGTAGCAATCATACTTGCTTTGTCCAAGACATGGATATCAGAGGCTAAGCAAAGAAAATTGGTATTGAATGTTGGTGGACCGGACCGGGTATCCCGTGTTCAAATGGCTGAGACAGTTGCTGATATAAGGGGATACAACCTCTCATTAATTAAATCTGTATCTTCATCATCGCTTGATCGTGGAGTTATGTCTCCTGCTGACATATCCATGGATATAACTAAGTTAGTTCAGACGCTTGGTATTTCTCCTATTTCATTTCGAGATGGTGTCAGATTGACGCTTGAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RmlD_sub_bind

Protein Analysis

318

Amino Acids

34.66

Weight (kDa)

5.55

Isoelectric Point (pI)

45.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 642
AccB7I CCANNNNNTGG 1 cut(s) 696
AccI GTMKAC 1 cut(s) 401
AciI CCGC 1 cut(s) 227
AclWI GGATC 3 cut(s) 278, 291, 361
AcsI RAATTY 1 cut(s) 620
AfiI CCNNNNNNNGG 1 cut(s) 696
AgeI ACCGGT 1 cut(s) 556
AgsI TTSAA 7 cut(s) 80, 184, 203, 564, 733, 767, 950
AluBI AGCT 4 cut(s) 137, 296, 342, 476
AluI AGCT 4 cut(s) 137, 296, 342, 476
Alw26I GTCTC 2 cut(s) 770, 861
AlwI GGATC 3 cut(s) 278, 291, 361
AlwNI CAGNNNCTG 1 cut(s) 785
AoxI GGCC 1 cut(s) 539
ApeKI GCWGC 5 cut(s) 163, 251, 290, 293, 473
ApoI RAATTY 1 cut(s) 620
AseI ATTAAT 1 cut(s) 812
AsiGI ACCGGT 1 cut(s) 556
Asp700I GAANNNNTTC 2 cut(s) 438, 624
AspS9I GGNCC 4 cut(s) 224, 539, 743, 748
AsuC2I CCSGG 1 cut(s) 752
AsuHPI GGTGA 1 cut(s) 546
AvaII GGWCC 3 cut(s) 224, 743, 748
BaeGI GKGCMC 1 cut(s) 647
BamHI GGATCC 1 cut(s) 283
BanI GGYRCC 1 cut(s) 642
BauI CACGAG 1 cut(s) 267
BbvI GCAGC 5 cut(s) 150, 238, 277, 305, 485
BccI CCATC 3 cut(s) 322, 584, 923
BciVI GTATCC 2 cut(s) 766, 791
BclI TGATCA 1 cut(s) 382
BcnI CCSGG 1 cut(s) 752
BcoDI GTCTC 2 cut(s) 770, 861
BfaI CTAG 1 cut(s) 156
BfmI CTRYAG 2 cut(s) 291, 402
BfuI GTATCC 2 cut(s) 766, 791
BisI GCNGC 5 cut(s) 164, 252, 291, 294, 474
BlpI GCTNAGC 2 cut(s) 477, 711
BlsI GCNGC 5 cut(s) 165, 253, 292, 295, 475
Bme1390I CCNGG 1 cut(s) 752
Bme18I GGWCC 3 cut(s) 224, 743, 748
BmgT120I GGNCC 4 cut(s) 224, 539, 743, 748
BmiI GGNNCC 3 cut(s) 264, 285, 644
BmrFI CCNGG 1 cut(s) 752
BplI GAGNNNNNCTC 2 cut(s) 485, 517
Bpu1102I GCTNAGC 2 cut(s) 477, 711
BpuEI CTTGAG 1 cut(s) 103
BpuMI CCSGG 1 cut(s) 752
BsaJI CCNNGG 1 cut(s) 873
BsaWI WCCGGW 2 cut(s) 556, 745
Bsc4I CCNNNNNNNGG 1 cut(s) 696
Bse118I RCCGGY 1 cut(s) 556
Bse1I ACTGG 1 cut(s) 443
Bse3DI GCAATG 1 cut(s) 199
BseDI CCNNGG 1 cut(s) 873
BseGI GGATG 1 cut(s) 667
BseLI CCNNNNNNNGG 1 cut(s) 696
BseMI GCAATG 1 cut(s) 199
BseMII CTCAG 3 cut(s) 468, 483, 765
BseNI ACTGG 1 cut(s) 443
BseRI GAGGAG 1 cut(s) 117
BseSI GKGCMC 1 cut(s) 647
BseXI GCAGC 5 cut(s) 150, 238, 277, 305, 485
BsgI GTGCAG 1 cut(s) 237
BshFI GGCC 1 cut(s) 541
BshNI GGYRCC 1 cut(s) 642
BshTI ACCGGT 1 cut(s) 556
BsiSI CCGG 3 cut(s) 557, 746, 751
BslFI GGGAC 1 cut(s) 60
BslI CCNNNNNNNGG 1 cut(s) 696
BsmAI GTCTC 2 cut(s) 770, 861
BsmFI GGGAC 1 cut(s) 60
BsnI GGCC 1 cut(s) 541
Bsp1286I GDGCHC 1 cut(s) 647
Bsp143I GATC 5 cut(s) 97, 283, 366, 382, 841
Bsp1720I GCTNAGC 2 cut(s) 477, 711
Bsp19I CCATGG 1 cut(s) 873
BspACI CCGC 1 cut(s) 227
BspANI GGCC 1 cut(s) 541
BspCNI CTCAG 3 cut(s) 469, 484, 766
BspHI TCATGA 1 cut(s) 628
BspLI GGNNCC 3 cut(s) 264, 285, 644
BspMAI CTGCAG 1 cut(s) 295
BspPI GGATC 3 cut(s) 278, 291, 361
BspT107I GGYRCC 1 cut(s) 642
BsrDI GCAATG 1 cut(s) 199
BsrFI RCCGGY 1 cut(s) 556
BsrI ACTGG 1 cut(s) 443
BssAI RCCGGY 1 cut(s) 556
BssECI CCNNGG 1 cut(s) 873
BssMI GATC 5 cut(s) 97, 283, 366, 382, 841
BssSI CACGAG 1 cut(s) 267
BssT1I CCWWGG 1 cut(s) 873
Bst2BI CACGAG 1 cut(s) 267
Bst4CI ACNGT 4 cut(s) 248, 406, 781, 954
Bst6I CTCTTC 3 cut(s) 5, 342, 576
BstAPI GCANNNNNTGC 1 cut(s) 58
BstDEI CTNAG 6 cut(s) 343, 477, 492, 711, 774, 885
BstDSI CCRYGG 1 cut(s) 873
BstF5I GGATG 1 cut(s) 667
BstKTI GATC 5 cut(s) 100, 286, 369, 385, 844
BstMAI GTCTC 2 cut(s) 770, 861
BstMBI GATC 5 cut(s) 97, 283, 366, 382, 841
BstMWI GCNNNNNNNGC 1 cut(s) 58
BstNSI RCATGY 1 cut(s) 56
BstSCI CCNGG 1 cut(s) 750
BstSFI CTRYAG 2 cut(s) 291, 402
BstSLI GKGCMC 1 cut(s) 647
BstV1I GCAGC 5 cut(s) 150, 238, 277, 305, 485
BstX2I RGATCY 1 cut(s) 283
BstYI RGATCY 1 cut(s) 283
BsuI GTATCC 2 cut(s) 766, 791
BsuRI GGCC 1 cut(s) 541
BtgI CCRYGG 1 cut(s) 873
BtgZI GCGATG 1 cut(s) 819
BtsCI GGATG 1 cut(s) 667
BtsIMutI CAGTG 1 cut(s) 587
CaiI CAGNNNCTG 1 cut(s) 785
CciI TCATGA 1 cut(s) 628
Cfr10I RCCGGY 1 cut(s) 556
Cfr13I GGNCC 4 cut(s) 224, 539, 743, 748
CpoI CGGWCCG 1 cut(s) 748
CseI GACGC 2 cut(s) 907, 952
CspAI ACCGGT 1 cut(s) 556
CspI CGGWCCG 1 cut(s) 748
CviAII CATG 4 cut(s) 53, 629, 696, 874
CviJI RGCY 9 cut(s) 66, 137, 206, 296, 342, 476, 541, 710, 773
CviKI_1 RGCY 9 cut(s) 66, 137, 206, 296, 342, 476, 541, 710, 773
DdeI CTNAG 6 cut(s) 343, 477, 492, 711, 774, 885
DpnI GATC 5 cut(s) 99, 285, 368, 384, 843
DpnII GATC 5 cut(s) 97, 283, 366, 382, 841
Eam1104I CTCTTC 3 cut(s) 5, 342, 576
EarI CTCTTC 3 cut(s) 5, 342, 576
EciI GGCGGA 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 873
Eco32I GATATC 1 cut(s) 702
Eco47I GGWCC 3 cut(s) 224, 743, 748
EcoRI GAATTC 1 cut(s) 620
EcoRV GATATC 1 cut(s) 702
EcoT14I CCWWGG 1 cut(s) 873
EcoT22I ATGCAT 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 873
FaeI CATG 4 cut(s) 56, 632, 699, 877
FaqI GGGAC 1 cut(s) 60
FatI CATG 4 cut(s) 52, 628, 695, 873
FbaI TGATCA 1 cut(s) 382
FblI GTMKAC 1 cut(s) 401
Fnu4HI GCNGC 5 cut(s) 164, 252, 291, 294, 474
FokI GGATG 1 cut(s) 674
Fsp4HI GCNGC 5 cut(s) 164, 252, 291, 294, 474
FspBI CTAG 1 cut(s) 156
GluI GCNGC 5 cut(s) 164, 252, 291, 294, 474
HaeIII GGCC 1 cut(s) 541
HapII CCGG 3 cut(s) 557, 746, 751
HgaI GACGC 2 cut(s) 907, 952
Hin1II CATG 4 cut(s) 56, 632, 699, 877
HincII GTYRAC 1 cut(s) 378
HindII GTYRAC 1 cut(s) 378
HindIII AAGCTT 1 cut(s) 135
HinfI GANTC 2 cut(s) 76, 577
HpaII CCGG 3 cut(s) 557, 746, 751
HphI GGTGA 1 cut(s) 546
Hpy166II GTNNAC 4 cut(s) 244, 378, 402, 743
Hpy188I TCNGA 6 cut(s) 73, 366, 576, 706, 897, 938
Hpy188III TCNNGA 4 cut(s) 80, 386, 629, 926
Hpy8I GTNNAC 4 cut(s) 244, 378, 402, 743
HpyCH4III ACNGT 4 cut(s) 248, 406, 781, 954
HpyCH4V TGCA 5 cut(s) 61, 149, 254, 293, 512
HpyF10VI GCNNNNNNNGC 1 cut(s) 58
HpyF3I CTNAG 6 cut(s) 343, 477, 492, 711, 774, 885
Hsp92II CATG 4 cut(s) 56, 632, 699, 877
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 5 cut(s) 97, 283, 366, 382, 841
Lsp1109I GCAGC 5 cut(s) 150, 238, 277, 305, 485
MaeI CTAG 1 cut(s) 156
MaeIII GTNAC 1 cut(s) 38
MalI GATC 5 cut(s) 99, 285, 368, 384, 843
MboI GATC 5 cut(s) 97, 283, 366, 382, 841
MboII GAAGA 7 cut(s) 22, 309, 353, 359, 437, 563, 819
MflI RGATCY 1 cut(s) 283
MhlI GDGCHC 1 cut(s) 647
MluCI AATT 6 cut(s) 328, 355, 513, 620, 722, 813
MmeI TCCRAC 1 cut(s) 7
MnlI CCTC 7 cut(s) 23, 135, 138, 276, 608, 700, 815
Mph1103I ATGCAT 1 cut(s) 151
MroXI GAANNNNTTC 2 cut(s) 438, 624
MseI TTAA 4 cut(s) 306, 327, 812, 816
MslI CAYNNNNRTG 1 cut(s) 872
MspA1I CMGCKG 1 cut(s) 476
MspI CCGG 3 cut(s) 557, 746, 751
MspR9I CCNGG 1 cut(s) 752
MwoI GCNNNNNNNGC 1 cut(s) 58
NciI CCSGG 1 cut(s) 752
NcoI CCATGG 1 cut(s) 873
NdeII GATC 5 cut(s) 97, 283, 366, 382, 841
NlaIII CATG 4 cut(s) 56, 632, 699, 877
NlaIV GGNNCC 3 cut(s) 264, 285, 644
NsiI ATGCAT 1 cut(s) 151
NspI RCATGY 1 cut(s) 56
PacI TTAATTAA 1 cut(s) 816
PagI TCATGA 1 cut(s) 628
PdmI GAANNNNTTC 2 cut(s) 438, 624
PfeI GAWTC 2 cut(s) 76, 577
PflMI CCANNNNNTGG 1 cut(s) 696
PinAI ACCGGT 1 cut(s) 556
PkrI GCNGC 5 cut(s) 165, 253, 292, 295, 475
PshBI ATTAAT 1 cut(s) 812
PspN4I GGNNCC 3 cut(s) 264, 285, 644
PspPI GGNCC 4 cut(s) 224, 539, 743, 748
PsrI GAACNNNNNNTAC 2 cut(s) 747, 779
PstI CTGCAG 1 cut(s) 295
PstNI CAGNNNCTG 1 cut(s) 785
PsuI RGATCY 1 cut(s) 283
PvuII CAGCTG 1 cut(s) 476
RseI CAYNNNNRTG 1 cut(s) 872
Rsr2I CGGWCCG 1 cut(s) 748
RsrII CGGWCCG 1 cut(s) 748
SaqAI TTAA 4 cut(s) 306, 327, 812, 816
SatI GCNGC 5 cut(s) 164, 252, 291, 294, 474
Sau3AI GATC 5 cut(s) 97, 283, 366, 382, 841
Sau96I GGNCC 4 cut(s) 224, 539, 743, 748
ScrFI CCNGG 1 cut(s) 752
SduI GDGCHC 1 cut(s) 647
SetI ASST 6 cut(s) 40, 139, 298, 344, 478, 807
SfcI CTRYAG 2 cut(s) 291, 402
SinI GGWCC 3 cut(s) 224, 743, 748
SmiMI CAYNNNNRTG 1 cut(s) 872
SmlI CTYRAG 1 cut(s) 118
SmoI CTYRAG 1 cut(s) 118
Sse9I AATT 6 cut(s) 328, 355, 513, 620, 722, 813
SsiI CCGC 1 cut(s) 227
SspMI CTAG 1 cut(s) 156
StyD4I CCNGG 1 cut(s) 750
StyI CCWWGG 1 cut(s) 873
TaaI ACNGT 4 cut(s) 248, 406, 781, 954
TaqI TCGA 2 cut(s) 338, 925
TasI AATT 6 cut(s) 328, 355, 513, 620, 722, 813
TfiI GAWTC 2 cut(s) 76, 577
Tru1I TTAA 4 cut(s) 306, 327, 812, 816
Tru9I TTAA 4 cut(s) 306, 327, 812, 816
TscAI CASTG 1 cut(s) 587
TseI GCWGC 5 cut(s) 163, 251, 290, 293, 473
TspDTI ATGAA 5 cut(s) 447, 475, 617, 819, 909
TspRI CASTG 1 cut(s) 587
Van91I CCANNNNNTGG 1 cut(s) 696
VpaK11BI GGWCC 3 cut(s) 224, 743, 748
VspI ATTAAT 1 cut(s) 812
XapI RAATTY 1 cut(s) 620
XceI RCATGY 1 cut(s) 56
XmiI GTMKAC 1 cut(s) 401
XmnI GAANNNNTTC 2 cut(s) 438, 624
XspI CTAG 1 cut(s) 156
Zsp2I ATGCAT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.