Rmu_sc0004191.1_g000013

cytochrome P450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004191.1
OG0002278
Physical Location & Seq
Forward (+)
38221 .. 38943
723 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 603 bp
atggattttctttccactcctttacctcccattgcaataggtttatgtgcagtacttgttttggcagtaatctttctgcggaagaagaccaagaaataccctcctgttgccggaactgtcatacaccaactgatcaatgtgtcaaggctgcaccattacatgactgagcttgcatgcaagtacaaaacttataggatgctcgacttgttcagatatgctgtctacactgtagatcctgcaaatgttgaatacattctcaaaacaaactttgcaaactatggcaaggtatgtaaaggatcatttgaagaggccgtggagagggttttgatccggcaggaggaggaggacatatttgtcttccaatttaaggagatggaggtgaagaatcgggttttctcggggggtccttggttctataacaactccatgctattgttggcagattacgacggagtttctgacctagaagcagcgctgctgcatctccttgaggtgtgggtggctgtgaaagggttgcggatagcgatgcagaatgagaaagccctaaccctaatcggacgagcactgggggactttgttcgagttgatcagggggcggtgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

200

Amino Acids

22.77

Weight (kDa)

7.69

Isoelectric Point (pI)

27.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 105 - 150 1e-05 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 353
AccI GTMKAC 1 cut(s) 222
AciI CCGC 3 cut(s) 79, 517, 596
AclWI GGATC 3 cut(s) 227, 304, 322
AfaI GTAC 2 cut(s) 54, 182
AfeI AGCGCT 1 cut(s) 474
AfiI CCNNNNNNNGG 4 cut(s) 110, 318, 337, 367
AgsI TTSAA 2 cut(s) 248, 305
AluBI AGCT 1 cut(s) 169
AluI AGCT 1 cut(s) 169
Alw21I GWGCWC 1 cut(s) 565
AlwI GGATC 3 cut(s) 227, 304, 322
Ama87I CYCGRG 1 cut(s) 397
Aor51HI AGCGCT 1 cut(s) 474
AoxI GGCC 1 cut(s) 309
ApeKI GCWGC 4 cut(s) 148, 470, 475, 478
Asp700I GAANNNNTTC 1 cut(s) 252
AspLEI GCGC 1 cut(s) 475
AspS9I GGNCC 1 cut(s) 404
AsuHPI GGTGA 1 cut(s) 391
AvaI CYCGRG 1 cut(s) 397
AvaII GGWCC 1 cut(s) 404
BbsI GAAGAC 2 cut(s) 92, 349
Bbv12I GWGCWC 1 cut(s) 565
BbvI GCAGC 4 cut(s) 135, 462, 465, 482
BccI CCATC 1 cut(s) 367
BceAI ACGGC 1 cut(s) 296
BclI TGATCA 2 cut(s) 132, 586
BfaI CTAG 1 cut(s) 464
BfmI CTRYAG 1 cut(s) 228
BfoI RGCGCY 1 cut(s) 476
BisI GCNGC 4 cut(s) 149, 471, 476, 479
BlsI GCNGC 4 cut(s) 150, 472, 477, 480
BmcAI AGTACT 1 cut(s) 54
Bme18I GGWCC 1 cut(s) 404
BmeT110I CYCGRG 1 cut(s) 397
BmgT120I GGNCC 1 cut(s) 404
BmiI GGNNCC 1 cut(s) 405
BmrI ACTGGG 1 cut(s) 575
BmsI GCATC 3 cut(s) 186, 490, 516
BmuI ACTGGG 1 cut(s) 575
BpiI GAAGAC 2 cut(s) 92, 349
BpuEI CTTGAG 1 cut(s) 509
BsaJI CCNNGG 2 cut(s) 312, 407
Bsc4I CCNNNNNNNGG 4 cut(s) 110, 318, 337, 367
Bse1I ACTGG 1 cut(s) 570
Bse3DI GCAATG 1 cut(s) 30
BseDI CCNNGG 2 cut(s) 312, 407
BseGI GGATG 1 cut(s) 201
BseLI CCNNNNNNNGG 4 cut(s) 110, 318, 337, 367
BseMI GCAATG 1 cut(s) 30
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 1 cut(s) 570
BseRI GAGGAG 2 cut(s) 353, 356
BseXI GCAGC 4 cut(s) 135, 462, 465, 482
BsgI GTGCAG 2 cut(s) 69, 134
BshFI GGCC 1 cut(s) 311
BsiHKAI GWGCWC 1 cut(s) 565
BsiHKCI CYCGRG 1 cut(s) 397
BsiSI CCGG 2 cut(s) 111, 331
BslFI GGGAC 1 cut(s) 584
BslI CCNNNNNNNGG 4 cut(s) 110, 318, 337, 367
BsmFI GGGAC 1 cut(s) 584
BsnI GGCC 1 cut(s) 311
BsoBI CYCGRG 1 cut(s) 397
Bsp1286I GDGCHC 1 cut(s) 565
Bsp143I GATC 5 cut(s) 132, 232, 296, 327, 586
BspACI CCGC 3 cut(s) 79, 517, 596
BspANI GGCC 1 cut(s) 311
BspCNI CTCAG 1 cut(s) 157
BspLI GGNNCC 1 cut(s) 405
BspPI GGATC 3 cut(s) 227, 304, 322
BsrDI GCAATG 1 cut(s) 30
BsrI ACTGG 1 cut(s) 570
BssECI CCNNGG 2 cut(s) 312, 407
BssMI GATC 5 cut(s) 132, 232, 296, 327, 586
BssT1I CCWWGG 1 cut(s) 407
Bst4CI ACNGT 2 cut(s) 118, 229
Bst6I CTCTTC 1 cut(s) 300
BstC8I GCNNGC 2 cut(s) 171, 175
BstDEI CTNAG 1 cut(s) 165
BstDSI CCRYGG 1 cut(s) 312
BstF5I GGATG 1 cut(s) 201
BstH2I RGCGCY 1 cut(s) 476
BstHHI GCGC 1 cut(s) 475
BstKTI GATC 5 cut(s) 135, 235, 299, 330, 589
BstMBI GATC 5 cut(s) 132, 232, 296, 327, 586
BstNSI RCATGY 1 cut(s) 177
BstSFI CTRYAG 1 cut(s) 228
BstV1I GCAGC 4 cut(s) 135, 462, 465, 482
BstV2I GAAGAC 2 cut(s) 92, 349
BstX2I RGATCY 1 cut(s) 232
BstYI RGATCY 1 cut(s) 232
BsuRI GGCC 1 cut(s) 311
BtgI CCRYGG 1 cut(s) 312
BtgZI GCGATG 1 cut(s) 539
BtsCI GGATG 1 cut(s) 201
BtsIMutI CAGTG 2 cut(s) 225, 563
Cac8I GCNNGC 2 cut(s) 171, 175
CfoI GCGC 1 cut(s) 475
Cfr13I GGNCC 1 cut(s) 404
Csp6I GTAC 2 cut(s) 53, 181
CviAII CATG 3 cut(s) 160, 174, 427
CviJI RGCY 5 cut(s) 148, 169, 311, 503, 542
CviKI_1 RGCY 5 cut(s) 148, 169, 311, 503, 542
CviQI GTAC 2 cut(s) 53, 181
DdeI CTNAG 1 cut(s) 165
DpnI GATC 5 cut(s) 134, 234, 298, 329, 588
DpnII GATC 5 cut(s) 132, 232, 296, 327, 586
DrdI GACNNNNNNGTC 1 cut(s) 353
DseDI GACNNNNNNGTC 1 cut(s) 353
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
Eco130I CCWWGG 1 cut(s) 407
Eco47I GGWCC 1 cut(s) 404
Eco47III AGCGCT 1 cut(s) 474
Eco88I CYCGRG 1 cut(s) 397
EcoO109I RGGNCCY 1 cut(s) 404
EcoT14I CCWWGG 1 cut(s) 407
ErhI CCWWGG 1 cut(s) 407
FaeI CATG 3 cut(s) 163, 177, 430
FaqI GGGAC 1 cut(s) 584
FatI CATG 3 cut(s) 159, 173, 426
FbaI TGATCA 2 cut(s) 132, 586
FblI GTMKAC 1 cut(s) 222
Fnu4HI GCNGC 4 cut(s) 149, 471, 476, 479
FokI GGATG 1 cut(s) 208
Fsp4HI GCNGC 4 cut(s) 149, 471, 476, 479
FspBI CTAG 1 cut(s) 464
GlaI GCGC 1 cut(s) 474
GluI GCNGC 4 cut(s) 149, 471, 476, 479
HaeII RGCGCY 1 cut(s) 476
HaeIII GGCC 1 cut(s) 311
HapII CCGG 2 cut(s) 111, 331
HhaI GCGC 1 cut(s) 475
Hin1II CATG 3 cut(s) 163, 177, 430
Hin6I GCGC 1 cut(s) 473
HinP1I GCGC 1 cut(s) 473
HinfI GANTC 1 cut(s) 385
HpaII CCGG 2 cut(s) 111, 331
HphI GGTGA 1 cut(s) 391
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 3 cut(s) 212, 460, 557
Hpy8I GTNNAC 1 cut(s) 223
Hpy99I CGWCG 1 cut(s) 452
HpyCH4III ACNGT 2 cut(s) 118, 229
HpyCH4V TGCA 9 cut(s) 35, 50, 151, 173, 177, 239, 272, 481, 529
HpyF3I CTNAG 1 cut(s) 165
Hsp92II CATG 3 cut(s) 163, 177, 430
HspAI GCGC 1 cut(s) 473
Ksp22I TGATCA 2 cut(s) 132, 586
Kzo9I GATC 5 cut(s) 132, 232, 296, 327, 586
LpnPI CCDG 7 cut(s) 117, 124, 249, 320, 344, 551, 575
Lsp1109I GCAGC 4 cut(s) 135, 462, 465, 482
LweI GCATC 3 cut(s) 186, 490, 516
MaeI CTAG 1 cut(s) 464
MalI GATC 5 cut(s) 134, 234, 298, 329, 588
MboI GATC 5 cut(s) 132, 232, 296, 327, 586
MboII GAAGA 5 cut(s) 94, 97, 317, 349, 394
MflI RGATCY 1 cut(s) 232
MhlI GDGCHC 1 cut(s) 565
MluCI AATT 1 cut(s) 362
MnlI CCTC 9 cut(s) 36, 111, 301, 312, 331, 334, 337, 370, 484
MroXI GAANNNNTTC 1 cut(s) 252
MseI TTAA 1 cut(s) 366
MspI CCGG 2 cut(s) 111, 331
NdeII GATC 5 cut(s) 132, 232, 296, 327, 586
NlaIII CATG 3 cut(s) 163, 177, 430
NlaIV GGNNCC 1 cut(s) 405
NspI RCATGY 1 cut(s) 177
PaeI GCATGC 1 cut(s) 177
PdmI GAANNNNTTC 1 cut(s) 252
PfeI GAWTC 1 cut(s) 385
PkrI GCNGC 4 cut(s) 150, 472, 477, 480
PpuMI RGGWCCY 1 cut(s) 404
Psp5II RGGWCCY 1 cut(s) 404
PspN4I GGNNCC 1 cut(s) 405
PspPI GGNCC 1 cut(s) 404
PspPPI RGGWCCY 1 cut(s) 404
PsuI RGATCY 1 cut(s) 232
RsaI GTAC 2 cut(s) 54, 182
RsaNI GTAC 2 cut(s) 53, 181
SaqAI TTAA 1 cut(s) 366
SatI GCNGC 4 cut(s) 149, 471, 476, 479
Sau3AI GATC 5 cut(s) 132, 232, 296, 327, 586
Sau96I GGNCC 1 cut(s) 404
ScaI AGTACT 1 cut(s) 54
SduI GDGCHC 1 cut(s) 565
SetI ASST 7 cut(s) 28, 43, 171, 288, 381, 465, 495
SfaNI GCATC 3 cut(s) 186, 490, 516
SfcI CTRYAG 1 cut(s) 228
SinI GGWCC 1 cut(s) 404
SmlI CTYRAG 1 cut(s) 488
SmoI CTYRAG 1 cut(s) 488
SphI GCATGC 1 cut(s) 177
Sse9I AATT 1 cut(s) 362
SsiI CCGC 3 cut(s) 79, 517, 596
SspMI CTAG 1 cut(s) 464
StyI CCWWGG 1 cut(s) 407
TaaI ACNGT 2 cut(s) 118, 229
TaqI TCGA 2 cut(s) 201, 580
TasI AATT 1 cut(s) 362
TatI WGTACW 2 cut(s) 52, 180
TfiI GAWTC 1 cut(s) 385
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TscAI CASTG 2 cut(s) 232, 570
TseI GCWGC 4 cut(s) 148, 470, 475, 478
TspGWI ACGGA 1 cut(s) 465
TspRI CASTG 2 cut(s) 232, 570
VpaK11BI GGWCC 1 cut(s) 404
XceI RCATGY 1 cut(s) 177
XcmI CCANNNNNNNNNTGG 1 cut(s) 433
XmiI GTMKAC 1 cut(s) 222
XmnI GAANNNNTTC 1 cut(s) 252
XspI CTAG 1 cut(s) 464
ZrmI AGTACT 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.