Rmu_sc0004695.1_g000003

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004695.1
OG0003355
Physical Location & Seq
Reverse (-)
8022 .. 10264
2243 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1089 bp
atgttgggcttccaagctcctttcttgggtggaactgatggcttcacgtcgggcttcctccacctcgtgttgggcttccaccacctcgagttggcggagctctatggaaaggactatgcattttggcatagctttcggagagatgagaaaatgtcacaactatgtggtctcttcctctcacgtcactttcggaccatgccctccgaccatacctatcatcagagcgtgacacctggcctaataggtaagagttggcccttgatttccgctaactatgtctcctttgcaatcactttgtctaagagtgatcctggtagtcgaggccttagccttgaaggcttaggtgaagaaacttcttgcccaagaaacatcgtccaaggccgagaagaatgtccaactcgagaagattcgctcgcagaaatggatgaaatcatctttgccacagttgacaagctttttgccaggaacagttcaatccctccatcacaaattgacattctcgtcgtcaatgtcttcatgcataatttgttcaagtcatacaagaattcatatgctgttgttgtcagcactgaatccatggctcctaataggtattgtgggatagaaaaatccatgatgctcacgaactgtttgtttcgggcaggagggtgttcgatgctgttcacaaatagaagatcccttcagcacctagcaatgttgaaattgaagtggttggtcagaacccatattggctcaagtgacaaggcttatgaatgttgcatacaacttgaagatgaaagtggacatctaggctttcgacttaccaaacacgtcacagaagcagcagctcttgctttcacagtgaacctccaagttttagctggaattcaacacttctgtctccaccctggtggaagagcaattataaatgggataggtaagagcttatcattaagcgactatgatcttgagccatctagaatggcgctttacagatttggtaacacaccagctgctggcttttggtatgctttgggatacatggaagccaaacaaaggcttaagaggggtgataggattctaatgagtgtggctttggagcagttttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

362

Amino Acids

40.52

Weight (kDa)

8.05

Isoelectric Point (pI)

48.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 111 - 175 4.9e-13 FAE1/Type III polyketide synthase-like protein
FAE1_CUT1_RppA PF08392 174 - 287 3e-39 FAE1/Type III polyketide synthase-like protein
Chal_sti_synt_C PF02797 292 - 346 9.1e-07 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 905
AasI GACNNNNNNGTC 1 cut(s) 500
AccB7I CCANNNNNTGG 1 cut(s) 995
AciI CCGC 2 cut(s) 95, 267
AclWI GGATC 2 cut(s) 302, 669
AcsI RAATTY 2 cut(s) 544, 864
AcuI CTGAAG 1 cut(s) 665
AdeI CACNNNGTG 1 cut(s) 67
AfiI CCNNNNNNNGG 5 cut(s) 26, 70, 91, 995, 1035
AflII CTTAAG 1 cut(s) 1040
AflIII ACRYGT 1 cut(s) 808
AgsI TTSAA 7 cut(s) 335, 474, 532, 700, 706, 770, 869
AjiI CACGTC 3 cut(s) 48, 182, 811
AjnI CCWGG 4 cut(s) 232, 310, 461, 886
AleI CACNNNNGTG 1 cut(s) 888
AluBI AGCT 8 cut(s) 17, 100, 132, 454, 827, 860, 924, 992
AluI AGCT 8 cut(s) 17, 100, 132, 454, 827, 860, 924, 992
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 3 cut(s) 173, 283, 884
AlwI GGATC 2 cut(s) 302, 669
AlwNI CAGNNNCTG 1 cut(s) 995
Ama87I CYCGRG 2 cut(s) 86, 399
AoxI GGCC 4 cut(s) 235, 254, 322, 379
ApeKI GCWGC 3 cut(s) 821, 824, 992
ApoI RAATTY 2 cut(s) 544, 864
ArsI GACNNNNNNTTYG 2 cut(s) 440, 472
AspLEI GCGC 1 cut(s) 967
AspS9I GGNCC 2 cut(s) 192, 255
AsuHPI GGTGA 2 cut(s) 356, 1061
AvaI CYCGRG 2 cut(s) 86, 399
AvaII GGWCC 1 cut(s) 192
BanII GRGCYC 1 cut(s) 102
BauI CACGAG 1 cut(s) 65
BbsI GAAGAC 1 cut(s) 505
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 3 cut(s) 833, 836, 979
BccI CCATC 3 cut(s) 32, 490, 961
BcgI CGANNNNNNTGC 2 cut(s) 116, 150
BciT130I CCWGG 4 cut(s) 234, 312, 463, 888
BciVI GTATCC 1 cut(s) 1010
BcoDI GTCTC 3 cut(s) 173, 283, 884
BfaI CTAG 3 cut(s) 689, 788, 957
BfoI RGCGCY 1 cut(s) 968
BfrI CTTAAG 1 cut(s) 1040
BfuI GTATCC 1 cut(s) 1010
BglI GCCNNNNNGGC 1 cut(s) 336
BisI GCNGC 3 cut(s) 822, 825, 993
BlsI GCNGC 3 cut(s) 823, 826, 994
Bme1390I CCNGG 4 cut(s) 234, 312, 463, 888
Bme18I GGWCC 1 cut(s) 192
BmeT110I CYCGRG 2 cut(s) 86, 399
BmgBI CACGTC 3 cut(s) 48, 182, 811
BmgT120I GGNCC 2 cut(s) 192, 255
BmiI GGNNCC 1 cut(s) 582
BmrFI CCNGG 4 cut(s) 234, 312, 463, 888
BmsI GCATC 2 cut(s) 606, 645
BpiI GAAGAC 1 cut(s) 505
Bpu10I CCTNAGC 2 cut(s) 326, 340
BpuEI CTTGAG 2 cut(s) 718, 968
BsaI GGTCTC 1 cut(s) 173
BsaJI CCNNGG 3 cut(s) 376, 576, 886
BsaXI ACNNNNNCTCC 2 cut(s) 263, 293
Bsc4I CCNNNNNNNGG 5 cut(s) 26, 70, 91, 995, 1035
Bse3DI GCAATG 1 cut(s) 699
BseBI CCWGG 4 cut(s) 234, 312, 463, 888
BseDI CCNNGG 3 cut(s) 376, 576, 886
BseGI GGATG 1 cut(s) 430
BseLI CCNNNNNNNGG 5 cut(s) 26, 70, 91, 995, 1035
BseMI GCAATG 1 cut(s) 699
BseXI GCAGC 3 cut(s) 833, 836, 979
BshFI GGCC 4 cut(s) 237, 256, 324, 381
BsiHKAI GWGCWC 1 cut(s) 102
BsiHKCI CYCGRG 2 cut(s) 86, 399
BslI CCNNNNNNNGG 5 cut(s) 26, 70, 91, 995, 1035
BsmAI GTCTC 3 cut(s) 173, 283, 884
BsnI GGCC 4 cut(s) 237, 256, 324, 381
Bso31I GGTCTC 1 cut(s) 173
BsoBI CYCGRG 2 cut(s) 86, 399
Bsp1286I GDGCHC 1 cut(s) 102
Bsp143I GATC 3 cut(s) 307, 674, 943
Bsp19I CCATGG 1 cut(s) 576
BspACI CCGC 2 cut(s) 95, 267
BspANI GGCC 4 cut(s) 237, 256, 324, 381
BspLI GGNNCC 1 cut(s) 582
BspPI GGATC 2 cut(s) 302, 669
BspQI GCTCTTC 1 cut(s) 889
BspTI CTTAAG 1 cut(s) 1040
BspTNI GGTCTC 1 cut(s) 173
BsrDI GCAATG 1 cut(s) 699
BssECI CCNNGG 3 cut(s) 376, 576, 886
BssMI GATC 3 cut(s) 307, 674, 943
BssSI CACGAG 1 cut(s) 65
BssT1I CCWWGG 2 cut(s) 376, 576
Bst2BI CACGAG 1 cut(s) 65
Bst2UI CCWGG 4 cut(s) 234, 312, 463, 888
Bst4CI ACNGT 4 cut(s) 445, 470, 629, 841
Bst6I CTCTTC 2 cut(s) 176, 889
BstAFI CTTAAG 1 cut(s) 1040
BstAPI GCANNNNNTGC 1 cut(s) 830
BstC8I GCNNGC 2 cut(s) 414, 997
BstDEI CTNAG 3 cut(s) 300, 326, 340
BstDSI CCRYGG 1 cut(s) 576
BstF5I GGATG 1 cut(s) 430
BstH2I RGCGCY 1 cut(s) 968
BstHHI GCGC 1 cut(s) 967
BstKTI GATC 3 cut(s) 310, 677, 946
BstMAI GTCTC 3 cut(s) 173, 283, 884
BstMBI GATC 3 cut(s) 307, 674, 943
BstMWI GCNNNNNNNGC 2 cut(s) 336, 830
BstNI CCWGG 4 cut(s) 234, 312, 463, 888
BstSCI CCNGG 4 cut(s) 232, 310, 461, 886
BstV1I GCAGC 3 cut(s) 833, 836, 979
BstV2I GAAGAC 1 cut(s) 505
BstX2I RGATCY 1 cut(s) 674
BstXI CCANNNNNNTGG 1 cut(s) 890
BstYI RGATCY 1 cut(s) 674
BsuI GTATCC 1 cut(s) 1010
BsuRI GGCC 4 cut(s) 237, 256, 324, 381
BtgI CCRYGG 1 cut(s) 576
BtrI CACGTC 3 cut(s) 48, 182, 811
BtsCI GGATG 1 cut(s) 430
BtsIMutI CAGTG 2 cut(s) 567, 846
Cac8I GCNNGC 2 cut(s) 414, 997
CaiI CAGNNNCTG 1 cut(s) 995
CfoI GCGC 1 cut(s) 967
Cfr13I GGNCC 2 cut(s) 192, 255
CviAII CATG 5 cut(s) 196, 517, 577, 613, 1021
DdeI CTNAG 3 cut(s) 300, 326, 340
DpnI GATC 3 cut(s) 309, 676, 945
DpnII GATC 3 cut(s) 307, 674, 943
DraIII CACNNNGTG 1 cut(s) 67
DrdI GACNNNNNNGTC 1 cut(s) 500
DseDI GACNNNNNNGTC 1 cut(s) 500
Eam1104I CTCTTC 2 cut(s) 176, 889
EarI CTCTTC 2 cut(s) 176, 889
EciI GGCGGA 1 cut(s) 110
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 2 cut(s) 376, 576
Eco147I AGGCCT 1 cut(s) 324
Eco24I GRGCYC 1 cut(s) 102
Eco31I GGTCTC 1 cut(s) 173
Eco47I GGWCC 1 cut(s) 192
Eco53kI GAGCTC 1 cut(s) 100
Eco57I CTGAAG 1 cut(s) 665
Eco88I CYCGRG 2 cut(s) 86, 399
EcoICRI GAGCTC 1 cut(s) 100
EcoRI GAATTC 2 cut(s) 544, 864
EcoRII CCWGG 4 cut(s) 232, 310, 461, 886
EcoT14I CCWWGG 2 cut(s) 376, 576
EcoT22I ATGCAT 2 cut(s) 121, 522
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 2 cut(s) 376, 576
FaeI CATG 5 cut(s) 199, 520, 580, 616, 1024
FatI CATG 5 cut(s) 195, 516, 576, 612, 1020
FauNDI CATATG 1 cut(s) 550
Fnu4HI GCNGC 3 cut(s) 822, 825, 993
FokI GGATG 1 cut(s) 437
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 3 cut(s) 822, 825, 993
FspBI CTAG 3 cut(s) 689, 788, 957
GlaI GCGC 1 cut(s) 966
GluI GCNGC 3 cut(s) 822, 825, 993
HaeII RGCGCY 1 cut(s) 968
HaeIII GGCC 4 cut(s) 237, 256, 324, 381
HhaI GCGC 1 cut(s) 967
Hin1II CATG 5 cut(s) 199, 520, 580, 616, 1024
Hin6I GCGC 1 cut(s) 965
HinP1I GCGC 1 cut(s) 965
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 3 cut(s) 407, 572, 1057
HphI GGTGA 2 cut(s) 356, 1061
Hpy166II GTNNAC 4 cut(s) 448, 663, 782, 844
Hpy188I TCNGA 5 cut(s) 138, 192, 205, 222, 719
Hpy188III TCNNGA 4 cut(s) 401, 622, 947, 957
Hpy8I GTNNAC 4 cut(s) 448, 663, 782, 844
Hpy99I CGWCG 2 cut(s) 52, 506
HpyAV CCTTC 2 cut(s) 329, 689
HpyCH4III ACNGT 4 cut(s) 445, 470, 629, 841
HpyCH4IV ACGT 3 cut(s) 47, 181, 810
HpyCH4V TGCA 4 cut(s) 119, 287, 520, 759
HpyF10VI GCNNNNNNNGC 2 cut(s) 336, 830
HpyF3I CTNAG 3 cut(s) 300, 326, 340
HpySE526I ACGT 3 cut(s) 47, 181, 810
Hsp92II CATG 5 cut(s) 199, 520, 580, 616, 1024
HspAI GCGC 1 cut(s) 965
Kzo9I GATC 3 cut(s) 307, 674, 943
LguI GCTCTTC 1 cut(s) 889
LmnI GCTCC 4 cut(s) 22, 97, 586, 1078
Lsp1109I GCAGC 3 cut(s) 833, 836, 979
LweI GCATC 2 cut(s) 606, 645
MaeI CTAG 3 cut(s) 689, 788, 957
MaeII ACGT 3 cut(s) 47, 181, 810
MaeIII GTNAC 6 cut(s) 153, 182, 226, 737, 811, 980
MalI GATC 3 cut(s) 309, 676, 945
MboI GATC 3 cut(s) 307, 674, 943
MboII GAAGA 8 cut(s) 163, 359, 398, 416, 505, 684, 782, 906
MflI RGATCY 1 cut(s) 674
MhlI GDGCHC 1 cut(s) 102
MluCI AATT 6 cut(s) 489, 523, 544, 701, 864, 900
MmeI TCCRAC 2 cut(s) 228, 419
Mph1103I ATGCAT 2 cut(s) 121, 522
MseI TTAA 3 cut(s) 932, 1041, 1087
MslI CAYNNNNRTG 2 cut(s) 160, 888
MspA1I CMGCKG 1 cut(s) 992
MspCI CTTAAG 1 cut(s) 1040
MspR9I CCNGG 4 cut(s) 234, 312, 463, 888
MvaI CCWGG 4 cut(s) 234, 312, 463, 888
MwoI GCNNNNNNNGC 2 cut(s) 336, 830
NcoI CCATGG 1 cut(s) 576
NdeI CATATG 1 cut(s) 550
NdeII GATC 3 cut(s) 307, 674, 943
NlaIII CATG 5 cut(s) 199, 520, 580, 616, 1024
NlaIV GGNNCC 1 cut(s) 582
NmeAIII GCCGAG 1 cut(s) 407
NmuCI GTSAC 5 cut(s) 153, 182, 226, 737, 811
NsiI ATGCAT 2 cut(s) 121, 522
OliI CACNNNNGTG 1 cut(s) 888
PaeR7I CTCGAG 2 cut(s) 86, 399
PceI AGGCCT 1 cut(s) 324
PciSI GCTCTTC 1 cut(s) 889
PfeI GAWTC 3 cut(s) 407, 572, 1057
PflMI CCANNNNNTGG 1 cut(s) 995
PkrI GCNGC 3 cut(s) 823, 826, 994
PsiI TTATAA 1 cut(s) 905
Psp124BI GAGCTC 1 cut(s) 102
Psp6I CCWGG 4 cut(s) 232, 310, 461, 886
PspGI CCWGG 4 cut(s) 232, 310, 461, 886
PspN4I GGNNCC 1 cut(s) 582
PspPI GGNCC 2 cut(s) 192, 255
PspXI VCTCGAGB 1 cut(s) 86
PstNI CAGNNNCTG 1 cut(s) 995
PsuI RGATCY 1 cut(s) 674
PvuII CAGCTG 1 cut(s) 992
RseI CAYNNNNRTG 2 cut(s) 160, 888
SacI GAGCTC 1 cut(s) 102
SapI GCTCTTC 1 cut(s) 889
SaqAI TTAA 3 cut(s) 932, 1041, 1087
SatI GCNGC 3 cut(s) 822, 825, 993
Sau3AI GATC 3 cut(s) 307, 674, 943
Sau96I GGNCC 2 cut(s) 192, 255
ScrFI CCNGG 4 cut(s) 234, 312, 463, 888
SduI GDGCHC 1 cut(s) 102
SfaNI GCATC 2 cut(s) 606, 645
Sfr274I CTCGAG 2 cut(s) 86, 399
SinI GGWCC 1 cut(s) 192
SlaI CTCGAG 2 cut(s) 86, 399
SmiMI CAYNNNNRTG 2 cut(s) 160, 888
SmlI CTYRAG 5 cut(s) 86, 399, 733, 947, 1040
SmoI CTYRAG 5 cut(s) 86, 399, 733, 947, 1040
Sse9I AATT 6 cut(s) 489, 523, 544, 701, 864, 900
SseBI AGGCCT 1 cut(s) 324
SsiI CCGC 2 cut(s) 95, 267
SspMI CTAG 3 cut(s) 689, 788, 957
SstI GAGCTC 1 cut(s) 102
StuI AGGCCT 1 cut(s) 324
StyD4I CCNGG 4 cut(s) 232, 310, 461, 886
StyI CCWWGG 2 cut(s) 376, 576
TaaI ACNGT 4 cut(s) 445, 470, 629, 841
TaiI ACGT 3 cut(s) 50, 184, 813
TaqI TCGA 5 cut(s) 87, 319, 400, 653, 796
TasI AATT 6 cut(s) 489, 523, 544, 701, 864, 900
TfiI GAWTC 3 cut(s) 407, 572, 1057
Tru1I TTAA 3 cut(s) 932, 1041, 1087
Tru9I TTAA 3 cut(s) 932, 1041, 1087
TscAI CASTG 2 cut(s) 574, 846
TseFI GTSAC 5 cut(s) 153, 182, 226, 737, 811
TseI GCWGC 3 cut(s) 821, 824, 992
Tsp45I GTSAC 5 cut(s) 153, 182, 226, 737, 811
TspDTI ATGAA 5 cut(s) 441, 505, 537, 765, 789
TspRI CASTG 2 cut(s) 574, 846
Van91I CCANNNNNTGG 1 cut(s) 995
Vha464I CTTAAG 1 cut(s) 1040
VpaK11BI GGWCC 1 cut(s) 192
XapI RAATTY 2 cut(s) 544, 864
XbaI TCTAGA 1 cut(s) 956
XcmI CCANNNNNNNNNTGG 1 cut(s) 857
XhoI CTCGAG 2 cut(s) 86, 399
XspI CTAG 3 cut(s) 689, 788, 957
Zsp2I ATGCAT 2 cut(s) 121, 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.