Rmu_sc0005139.1_g000009

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005139.1
OG0004243
Physical Location & Seq
Forward (+)
33751 .. 35449
1699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005139.1_g000009.1.cds

Sequence Viewer

Length: 576 bp
atgcctataaatacccttccaaatcctccaaaatacggagacgtctggaattcgaagaagacggaaaagagcgagagcgataacagcatgggcgaaaattcgcaggagatttacgaatctgaaactgtgttattcaagaagcagggtctctgcaaagaagagaatctgaaccaagtctggaagaaagaggtggaaacagtctttgaatctcatggcctttcatgcgaattgaactgggatgagtgtaagatgactgtcactgcaacagaacgtactgaaggtggtcttcttcaagatggctttgacaggggttttaatgctctctttgttctggcatgtggtctcggcactgaatggggcacaacattgcaggttgaaccaatcgtctccggtagtgtctatagtgatgtcatgaatattataataccaaatggcatgagtcaggaagcagctgttgttgttctttccgataaagccgggtcaacaagtacagtgaggaatcttgtgtatagttgtcttaatggcgatgatccttttgatgaagaccattttcatgaattgttccttgaagattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

21.26

Weight (kDa)

4.36

Isoelectric Point (pI)

45.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 422
AatII GACGTC 1 cut(s) 45
Acc36I ACCTGC 1 cut(s) 361
AclWI GGATC 1 cut(s) 524
AcsI RAATTY 2 cut(s) 49, 97
AcuI CTGAAG 1 cut(s) 297
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 2 cut(s) 274, 490
AfiI CCNNNNNNNGG 1 cut(s) 35
AgsI TTSAA 6 cut(s) 136, 206, 232, 293, 377, 569
AluBI AGCT 1 cut(s) 452
AluI AGCT 1 cut(s) 452
Alw26I GTCTC 4 cut(s) 33, 152, 347, 391
AlwI GGATC 1 cut(s) 524
AoxI GGCC 1 cut(s) 214
ApeKI GCWGC 1 cut(s) 449
ApoI RAATTY 2 cut(s) 49, 97
AsuC2I CCSGG 1 cut(s) 478
AsuII TTCGAA 1 cut(s) 53
BaeGI GKGCMC 1 cut(s) 362
BbsI GAAGAC 3 cut(s) 65, 278, 549
BbvI GCAGC 1 cut(s) 461
BccI CCATC 1 cut(s) 290
BcnI CCSGG 1 cut(s) 478
BcoDI GTCTC 4 cut(s) 33, 152, 347, 391
BfmI CTRYAG 1 cut(s) 400
BfuAI ACCTGC 1 cut(s) 361
BisI GCNGC 1 cut(s) 450
BlsI GCNGC 1 cut(s) 451
Bme1390I CCNGG 1 cut(s) 478
BmrFI CCNGG 1 cut(s) 478
BmrI ACTGGG 1 cut(s) 244
BmuI ACTGGG 1 cut(s) 244
BpiI GAAGAC 3 cut(s) 65, 278, 549
Bpu14I TTCGAA 1 cut(s) 53
BpuMI CCSGG 1 cut(s) 478
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 2 cut(s) 152, 347
BsaWI WCCGGW 1 cut(s) 389
Bsc4I CCNNNNNNNGG 1 cut(s) 35
Bse1I ACTGG 1 cut(s) 239
Bse3DI GCAATG 1 cut(s) 365
BseGI GGATG 1 cut(s) 244
BseLI CCNNNNNNNGG 1 cut(s) 35
BseMI GCAATG 1 cut(s) 365
BseNI ACTGG 1 cut(s) 239
BseSI GKGCMC 1 cut(s) 362
BseXI GCAGC 1 cut(s) 461
BshFI GGCC 1 cut(s) 216
BsiSI CCGG 2 cut(s) 390, 477
BslI CCNNNNNNNGG 1 cut(s) 35
BsmAI GTCTC 4 cut(s) 33, 152, 347, 391
BsmBI CGTCTC 2 cut(s) 33, 391
BsnI GGCC 1 cut(s) 216
Bso31I GGTCTC 2 cut(s) 152, 347
Bsp119I TTCGAA 1 cut(s) 53
Bsp1286I GDGCHC 1 cut(s) 362
Bsp143I GATC 1 cut(s) 529
BspANI GGCC 1 cut(s) 216
BspHI TCATGA 2 cut(s) 411, 553
BspMI ACCTGC 1 cut(s) 361
BspPI GGATC 1 cut(s) 524
BspT104I TTCGAA 1 cut(s) 53
BspTNI GGTCTC 2 cut(s) 152, 347
BsrDI GCAATG 1 cut(s) 365
BsrI ACTGG 1 cut(s) 239
BssMI GATC 1 cut(s) 529
BssNI GRCGYC 1 cut(s) 42
Bst4CI ACNGT 4 cut(s) 127, 199, 256, 493
Bst6I CTCTTC 1 cut(s) 153
BstACI GRCGYC 1 cut(s) 42
BstBI TTCGAA 1 cut(s) 53
BstF5I GGATG 1 cut(s) 244
BstKTI GATC 1 cut(s) 532
BstMAI GTCTC 4 cut(s) 33, 152, 347, 391
BstMBI GATC 1 cut(s) 529
BstMWI GCNNNNNNNGC 2 cut(s) 84, 222
BstNSI RCATGY 1 cut(s) 339
BstSCI CCNGG 1 cut(s) 476
BstSFI CTRYAG 1 cut(s) 400
BstSLI GKGCMC 1 cut(s) 362
BstV1I GCAGC 1 cut(s) 461
BstV2I GAAGAC 3 cut(s) 65, 278, 549
BsuRI GGCC 1 cut(s) 216
BtgZI GCGATG 1 cut(s) 540
BtsCI GGATG 1 cut(s) 244
BtsI GCAGTG 1 cut(s) 258
BtsIMutI CAGTG 3 cut(s) 258, 348, 498
BveI ACCTGC 1 cut(s) 361
CciI TCATGA 2 cut(s) 411, 553
Csp6I GTAC 2 cut(s) 273, 489
CviAII CATG 7 cut(s) 88, 212, 222, 336, 412, 436, 554
CviJI RGCY 4 cut(s) 216, 300, 452, 476
CviKI_1 RGCY 4 cut(s) 216, 300, 452, 476
CviQI GTAC 2 cut(s) 273, 489
DpnI GATC 1 cut(s) 531
DpnII GATC 1 cut(s) 529
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
Eco31I GGTCTC 2 cut(s) 152, 347
Eco57I CTGAAG 1 cut(s) 297
EcoRI GAATTC 1 cut(s) 49
Esp3I CGTCTC 2 cut(s) 33, 391
FaeI CATG 7 cut(s) 91, 215, 225, 339, 415, 439, 557
FalI AAGNNNNNCTT 2 cut(s) 270, 302
FatI CATG 7 cut(s) 87, 211, 221, 335, 411, 435, 553
Fnu4HI GCNGC 1 cut(s) 450
FokI GGATG 1 cut(s) 251
Fsp4HI GCNGC 1 cut(s) 450
GluI GCNGC 1 cut(s) 450
HaeIII GGCC 1 cut(s) 216
HapII CCGG 2 cut(s) 390, 477
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 7 cut(s) 91, 215, 225, 339, 415, 439, 557
HincII GTYRAC 1 cut(s) 483
HindII GTYRAC 1 cut(s) 483
HinfI GANTC 5 cut(s) 116, 163, 206, 439, 499
HpaII CCGG 2 cut(s) 390, 477
Hpy166II GTNNAC 1 cut(s) 483
Hpy188I TCNGA 3 cut(s) 121, 168, 469
Hpy188III TCNNGA 7 cut(s) 46, 136, 178, 293, 412, 443, 554
Hpy8I GTNNAC 1 cut(s) 483
HpyAV CCTTC 2 cut(s) 26, 272
HpyCH4III ACNGT 4 cut(s) 127, 199, 256, 493
HpyCH4IV ACGT 2 cut(s) 42, 271
HpyCH4V TGCA 3 cut(s) 153, 263, 370
HpyF10VI GCNNNNNNNGC 2 cut(s) 84, 222
HpySE526I ACGT 2 cut(s) 42, 271
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 7 cut(s) 91, 215, 225, 339, 415, 439, 557
Kzo9I GATC 1 cut(s) 529
Lsp1109I GCAGC 1 cut(s) 461
MaeII ACGT 2 cut(s) 42, 271
MaeIII GTNAC 1 cut(s) 256
MalI GATC 1 cut(s) 531
MboI GATC 1 cut(s) 529
MboII GAAGA 7 cut(s) 67, 70, 170, 193, 278, 281, 554
MhlI GDGCHC 1 cut(s) 362
MluCI AATT 4 cut(s) 49, 97, 227, 557
MlyI GAGTC 1 cut(s) 448
MnlI CCTC 3 cut(s) 36, 181, 489
MseI TTAA 2 cut(s) 315, 519
MslI CAYNNNNRTG 1 cut(s) 552
MspA1I CMGCKG 1 cut(s) 452
MspI CCGG 2 cut(s) 390, 477
MspR9I CCNGG 1 cut(s) 478
MwoI GCNNNNNNNGC 2 cut(s) 84, 222
NciI CCSGG 1 cut(s) 478
NdeII GATC 1 cut(s) 529
NlaIII CATG 7 cut(s) 91, 215, 225, 339, 415, 439, 557
NmeAIII GCCGAG 1 cut(s) 324
NmuCI GTSAC 1 cut(s) 256
NspI RCATGY 1 cut(s) 339
NspV TTCGAA 1 cut(s) 53
PagI TCATGA 2 cut(s) 411, 553
PfeI GAWTC 4 cut(s) 116, 163, 206, 499
PkrI GCNGC 1 cut(s) 451
PleI GAGTC 1 cut(s) 447
PpsI GAGTC 1 cut(s) 447
PsiI TTATAA 1 cut(s) 422
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 2 cut(s) 274, 490
RsaNI GTAC 2 cut(s) 273, 489
RseI CAYNNNNRTG 1 cut(s) 552
SaqAI TTAA 2 cut(s) 315, 519
SatI GCNGC 1 cut(s) 450
Sau3AI GATC 1 cut(s) 529
SchI GAGTC 1 cut(s) 448
ScrFI CCNGG 1 cut(s) 478
SduI GDGCHC 1 cut(s) 362
SetI ASST 6 cut(s) 45, 192, 274, 283, 375, 454
SfcI CTRYAG 1 cut(s) 400
SfuI TTCGAA 1 cut(s) 53
SmiMI CAYNNNNRTG 1 cut(s) 552
Sse9I AATT 4 cut(s) 49, 97, 227, 557
SspI AATATT 1 cut(s) 418
StyD4I CCNGG 1 cut(s) 476
TaaI ACNGT 4 cut(s) 127, 199, 256, 493
TaiI ACGT 2 cut(s) 45, 274
TaqI TCGA 1 cut(s) 53
TasI AATT 4 cut(s) 49, 97, 227, 557
TatI WGTACW 1 cut(s) 488
TfiI GAWTC 4 cut(s) 116, 163, 206, 499
Tru1I TTAA 2 cut(s) 315, 519
Tru9I TTAA 2 cut(s) 315, 519
TscAI CASTG 3 cut(s) 265, 355, 498
TseFI GTSAC 1 cut(s) 256
TseI GCWGC 1 cut(s) 449
Tsp45I GTSAC 1 cut(s) 256
TspDTI ATGAA 5 cut(s) 210, 428, 542, 555, 570
TspGWI ACGGA 2 cut(s) 51, 77
TspRI CASTG 3 cut(s) 265, 355, 498
XapI RAATTY 2 cut(s) 49, 97
XceI RCATGY 1 cut(s) 339
ZraI GACGTC 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.