Rroxscaffold_4G00276950

Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0004243
Physical Location & Seq
Forward (+)
465252 .. 467083
1832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00276950.1

Sequence Viewer

Length: 672 bp
ATGAATGCCTATAAATACTCTCGTCCTCTACCAAATCCTCCAAGATACGGAGACGTCTGGAATTCCAAGACGATGGAAAAGAGCGAGAGCGATAACAGTATGCACGAAAATTCGCAGAAGATCGACAACACTGGCTACGAATCAATGACTCTGTTATTCAAGAAGCAGGGTCTCTGCAAAGAAGAGAATCTGAACGAAGTGTGGAAGAAAGAGGTGGAAACAGTCTTTGAATCTCATGGCCTTTCATGCGAATTGAACTGGGATGAGTGTAAGATGACTGTTAGTGCAACAGAACGTACCCTGGACCGAAGGATCATTTACAGGGGTGCACGTGCTCTCTGTGTTCTGGCATGTGGTCTTGGCACTGAATGGGTTGAACCAATCGTCTCCGGCAGTGTCCATAGTGATGTCATGAAAATTACAATACCAGATGGCATGACTGAGGATGATTTTTCAAGCAAGTATCATGATTTCATATTCAAATTTGAAGACAAATTTGGACTACCTCGAAAGCTGTCCTGCTTTGTTCTTTATCAAGAAGCAGCTGTTGTTGTTCTTTCCGATAAATTCGGGTCAACAAGTATAGTGAGGAATCTTGTGACTAGTTGTCTTCTTGGCGATGATCCTTTTGATGAAGACCATTTTCATGAATTGTTCCTTGATGATGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

223

Amino Acids

25.54

Weight (kDa)

4.9

Isoelectric Point (pI)

33.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_KRR1_1st PF17903 60 - 118 4e-06 Krr1 KH1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 57
AclWI GGATC 2 cut(s) 320, 617
AcsI RAATTY 5 cut(s) 61, 109, 482, 494, 566
AcvI CACGTG 1 cut(s) 332
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 1 cut(s) 298
AfiI CCNNNNNNNGG 1 cut(s) 47
AgsI TTSAA 7 cut(s) 160, 230, 256, 377, 456, 481, 488
AhdI GACNNNNNGTC 1 cut(s) 606
AhlI ACTAGT 1 cut(s) 602
AjnI CCWGG 1 cut(s) 300
AjuI GAANNNNNNNTTGG 2 cut(s) 480, 512
AluBI AGCT 2 cut(s) 514, 545
AluI AGCT 2 cut(s) 514, 545
Alw21I GWGCWC 2 cut(s) 331, 337
Alw26I GTCTC 3 cut(s) 45, 176, 391
Alw44I GTGCAC 1 cut(s) 327
AlwI GGATC 2 cut(s) 320, 617
AoxI GGCC 1 cut(s) 238
ApaLI GTGCAC 1 cut(s) 327
ApeKI GCWGC 1 cut(s) 542
ApoI RAATTY 5 cut(s) 61, 109, 482, 494, 566
AspS9I GGNCC 1 cut(s) 304
AvaII GGWCC 1 cut(s) 304
BaeGI GKGCMC 1 cut(s) 331
BbrPI CACGTG 1 cut(s) 332
BbsI GAAGAC 3 cut(s) 495, 602, 642
Bbv12I GWGCWC 2 cut(s) 331, 337
BbvI GCAGC 1 cut(s) 554
BccI CCATC 2 cut(s) 67, 425
BciT130I CCWGG 1 cut(s) 302
BcoDI GTCTC 3 cut(s) 45, 176, 391
BcuI ACTAGT 1 cut(s) 602
BfaI CTAG 1 cut(s) 603
BisI GCNGC 1 cut(s) 543
BlsI GCNGC 1 cut(s) 544
Bme1390I CCNGG 1 cut(s) 302
Bme18I GGWCC 1 cut(s) 304
BmeRI GACNNNNNGTC 1 cut(s) 606
BmgT120I GGNCC 1 cut(s) 304
BmrFI CCNGG 1 cut(s) 302
BmrI ACTGGG 1 cut(s) 268
BmuI ACTGGG 1 cut(s) 268
BpiI GAAGAC 3 cut(s) 495, 602, 642
BsaAI YACGTR 1 cut(s) 332
BsaHI GRCGYC 1 cut(s) 54
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 1 cut(s) 300
Bsc4I CCNNNNNNNGG 1 cut(s) 47
Bse1I ACTGG 2 cut(s) 136, 263
BseBI CCWGG 1 cut(s) 302
BseDI CCNNGG 1 cut(s) 300
BseGI GGATG 2 cut(s) 268, 451
BseLI CCNNNNNNNGG 1 cut(s) 47
BseMII CTCAG 1 cut(s) 432
BseNI ACTGG 2 cut(s) 136, 263
BseSI GKGCMC 1 cut(s) 331
BseXI GCAGC 1 cut(s) 554
BshFI GGCC 1 cut(s) 240
BsiHKAI GWGCWC 2 cut(s) 331, 337
BsiSI CCGG 1 cut(s) 390
BslI CCNNNNNNNGG 1 cut(s) 47
BsmAI GTCTC 3 cut(s) 45, 176, 391
BsmBI CGTCTC 2 cut(s) 45, 391
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 1 cut(s) 240
Bso31I GGTCTC 1 cut(s) 176
Bsp1286I GDGCHC 2 cut(s) 331, 337
Bsp143I GATC 3 cut(s) 120, 312, 622
BspANI GGCC 1 cut(s) 240
BspCNI CTCAG 1 cut(s) 433
BspHI TCATGA 3 cut(s) 411, 466, 646
BspPI GGATC 2 cut(s) 320, 617
BspTNI GGTCTC 1 cut(s) 176
BsrI ACTGG 2 cut(s) 136, 263
BssECI CCNNGG 1 cut(s) 300
BssMI GATC 3 cut(s) 120, 312, 622
BssNI GRCGYC 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 302
Bst4CI ACNGT 3 cut(s) 98, 223, 280
Bst6I CTCTTC 1 cut(s) 177
BstACI GRCGYC 1 cut(s) 54
BstBAI YACGTR 1 cut(s) 332
BstDEI CTNAG 1 cut(s) 441
BstF5I GGATG 2 cut(s) 268, 451
BstKTI GATC 3 cut(s) 123, 315, 625
BstMAI GTCTC 3 cut(s) 45, 176, 391
BstMBI GATC 3 cut(s) 120, 312, 622
BstMWI GCNNNNNNNGC 1 cut(s) 246
BstNI CCWGG 1 cut(s) 302
BstNSI RCATGY 1 cut(s) 354
BstSCI CCNGG 1 cut(s) 300
BstSLI GKGCMC 1 cut(s) 331
BstV1I GCAGC 1 cut(s) 554
BstV2I GAAGAC 3 cut(s) 495, 602, 642
BstXI CCANNNNNNTGG 1 cut(s) 73
BsuRI GGCC 1 cut(s) 240
BtgZI GCGATG 1 cut(s) 633
BtsCI GGATG 2 cut(s) 268, 451
BtsI GCAGTG 1 cut(s) 400
BtsIMutI CAGTG 3 cut(s) 129, 363, 400
CciI TCATGA 3 cut(s) 411, 466, 646
Cfr13I GGNCC 1 cut(s) 304
Csp6I GTAC 1 cut(s) 297
CviAII CATG 7 cut(s) 236, 246, 351, 412, 436, 467, 647
CviJI RGCY 4 cut(s) 135, 240, 514, 545
CviKI_1 RGCY 4 cut(s) 135, 240, 514, 545
CviQI GTAC 1 cut(s) 297
DdeI CTNAG 1 cut(s) 441
DpnI GATC 3 cut(s) 122, 314, 624
DpnII GATC 3 cut(s) 120, 312, 622
DriI GACNNNNNGTC 1 cut(s) 606
Eam1104I CTCTTC 1 cut(s) 177
Eam1105I GACNNNNNGTC 1 cut(s) 606
EarI CTCTTC 1 cut(s) 177
Eco31I GGTCTC 1 cut(s) 176
Eco47I GGWCC 1 cut(s) 304
Eco72I CACGTG 1 cut(s) 332
EcoRI GAATTC 1 cut(s) 61
EcoRII CCWGG 1 cut(s) 300
Esp3I CGTCTC 2 cut(s) 45, 391
FaeI CATG 7 cut(s) 239, 249, 354, 415, 439, 470, 650
FatI CATG 7 cut(s) 235, 245, 350, 411, 435, 466, 646
Fnu4HI GCNGC 1 cut(s) 543
FokI GGATG 2 cut(s) 275, 458
Fsp4HI GCNGC 1 cut(s) 543
FspBI CTAG 1 cut(s) 603
GluI GCNGC 1 cut(s) 543
HaeIII GGCC 1 cut(s) 240
HapII CCGG 1 cut(s) 390
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 7 cut(s) 239, 249, 354, 415, 439, 470, 650
HincII GTYRAC 1 cut(s) 576
HindII GTYRAC 1 cut(s) 576
HinfI GANTC 5 cut(s) 140, 148, 187, 230, 592
HpaII CCGG 1 cut(s) 390
Hpy166II GTNNAC 2 cut(s) 329, 576
Hpy188I TCNGA 2 cut(s) 192, 562
Hpy188III TCNNGA 6 cut(s) 58, 160, 412, 467, 536, 647
Hpy8I GTNNAC 2 cut(s) 329, 576
HpyAV CCTTC 1 cut(s) 303
HpyCH4III ACNGT 3 cut(s) 98, 223, 280
HpyCH4IV ACGT 3 cut(s) 54, 295, 331
HpyCH4V TGCA 4 cut(s) 103, 177, 287, 329
HpyF10VI GCNNNNNNNGC 1 cut(s) 246
HpyF3I CTNAG 1 cut(s) 441
HpySE526I ACGT 3 cut(s) 54, 295, 331
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 7 cut(s) 239, 249, 354, 415, 439, 470, 650
Kzo9I GATC 3 cut(s) 120, 312, 622
Lsp1109I GCAGC 1 cut(s) 554
MaeI CTAG 1 cut(s) 603
MaeII ACGT 3 cut(s) 54, 295, 331
MaeIII GTNAC 1 cut(s) 598
MalI GATC 3 cut(s) 122, 314, 624
MboI GATC 3 cut(s) 120, 312, 622
MboII GAAGA 6 cut(s) 130, 194, 217, 500, 602, 647
MhlI GDGCHC 2 cut(s) 331, 337
MluCI AATT 8 cut(s) 61, 109, 251, 417, 482, 494, 566, 650
MlyI GAGTC 1 cut(s) 142
MnlI CCTC 6 cut(s) 36, 48, 205, 436, 516, 582
MseI TTAA 1 cut(s) 670
MslI CAYNNNNRTG 2 cut(s) 405, 645
MspA1I CMGCKG 1 cut(s) 545
MspI CCGG 1 cut(s) 390
MspR9I CCNGG 1 cut(s) 302
Mva1269I GAATGC 1 cut(s) 10
MvaI CCWGG 1 cut(s) 302
MwoI GCNNNNNNNGC 1 cut(s) 246
NdeII GATC 3 cut(s) 120, 312, 622
NlaIII CATG 7 cut(s) 239, 249, 354, 415, 439, 470, 650
NmuCI GTSAC 1 cut(s) 598
NspI RCATGY 1 cut(s) 354
PagI TCATGA 3 cut(s) 411, 466, 646
PctI GAATGC 1 cut(s) 10
PfeI GAWTC 4 cut(s) 140, 187, 230, 592
PkrI GCNGC 1 cut(s) 544
PleI GAGTC 1 cut(s) 142
PmaCI CACGTG 1 cut(s) 332
PmlI CACGTG 1 cut(s) 332
PpsI GAGTC 1 cut(s) 142
Ppu21I YACGTR 1 cut(s) 332
Psp6I CCWGG 1 cut(s) 300
PspCI CACGTG 1 cut(s) 332
PspGI CCWGG 1 cut(s) 300
PspPI GGNCC 1 cut(s) 304
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 1 cut(s) 298
RsaNI GTAC 1 cut(s) 297
RseI CAYNNNNRTG 2 cut(s) 405, 645
SaqAI TTAA 1 cut(s) 670
SatI GCNGC 1 cut(s) 543
Sau3AI GATC 3 cut(s) 120, 312, 622
Sau96I GGNCC 1 cut(s) 304
SchI GAGTC 1 cut(s) 142
ScrFI CCNGG 1 cut(s) 302
SduI GDGCHC 2 cut(s) 331, 337
SetI ASST 7 cut(s) 57, 216, 298, 334, 508, 516, 547
SinI GGWCC 1 cut(s) 304
SmiMI CAYNNNNRTG 2 cut(s) 405, 645
SpeI ACTAGT 1 cut(s) 602
Sse9I AATT 8 cut(s) 61, 109, 251, 417, 482, 494, 566, 650
SspMI CTAG 1 cut(s) 603
StyD4I CCNGG 1 cut(s) 300
TaaI ACNGT 3 cut(s) 98, 223, 280
TaiI ACGT 3 cut(s) 57, 298, 334
TaqI TCGA 2 cut(s) 123, 508
TaqII GACCGA 1 cut(s) 321
TasI AATT 8 cut(s) 61, 109, 251, 417, 482, 494, 566, 650
TfiI GAWTC 4 cut(s) 140, 187, 230, 592
Tru1I TTAA 1 cut(s) 670
Tru9I TTAA 1 cut(s) 670
TscAI CASTG 3 cut(s) 136, 370, 400
TseFI GTSAC 1 cut(s) 598
TseI GCWGC 1 cut(s) 542
Tsp45I GTSAC 1 cut(s) 598
TspDTI ATGAA 7 cut(s) 17, 234, 428, 463, 635, 648, 663
TspGWI ACGGA 1 cut(s) 63
TspRI CASTG 3 cut(s) 136, 370, 400
VneI GTGCAC 1 cut(s) 327
VpaK11BI GGWCC 1 cut(s) 304
XapI RAATTY 5 cut(s) 61, 109, 482, 494, 566
XceI RCATGY 1 cut(s) 354
XspI CTAG 1 cut(s) 603
ZraI GACGTC 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.