Rmu_sc0005767.1_g000024

Inner membrane component of T3SS, cytoplasmic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005767.1
OG0005560
Physical Location & Seq
Reverse (-)
104690 .. 108409
3720 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 735 bp
atgcgtgaagatattttagatcagcaggcatcacttcagcgagctagacttgaagcttctcttgctgatggcatctcttggggtatgggagaggatgccattatagaagaagctgaggatgatggtgaggaagtaacttggcaaacatacaaaggacagctttcggaaaagcagataaaaacccgtgataaaataacgaaaagaatggaaaagattgctcatatgaagaaagagatagatgctatttgtgctaaagacgtctctcaatgtggactgactcaagggcaacaaactcagatcgcgcgaaatgaacaaagaacagaacagattctggaagagcttgaaaatttggaagagactctgaatgagagtattcaagaaagtttacgtgcacatgttgggaagctatctcatggtaagaacaaaggagcaattgaagaagaagaagaagaacttttgagagaattgcagcagcacattttggaacttgctgatattcttgtggagagaccatcacaatatgcaaggagagtggaagaaatatctcttatgttcaagaacttgcatcatcttctgaactcattgcagccccatcaggctagggcaacactagttcacattctagaacttcatatcaaacgacgtaaacaagctgtggaggatgtaaagagaactttgcttattgatctcaaggtgtcatttgagttggtgccaacaggttgcatgtttctttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FHA

Protein Analysis

244

Amino Acids

28.12

Weight (kDa)

5.25

Isoelectric Point (pI)

61.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med7 PF05983 130 - 225 6e-18 MED7 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 261
AccB1I GGYRCC 1 cut(s) 709
AccII CGCG 2 cut(s) 302, 304
AcsI RAATTY 1 cut(s) 346
AcuI CTGAAG 1 cut(s) 20
AcyI GRCGYC 1 cut(s) 258
AflIII ACRYGT 1 cut(s) 394
AgsI TTSAA 5 cut(s) 53, 344, 377, 437, 556
AhlI ACTAGT 1 cut(s) 610
AluBI AGCT 7 cut(s) 44, 56, 113, 160, 340, 406, 653
AluI AGCT 7 cut(s) 44, 56, 113, 160, 340, 406, 653
Alw21I GWGCWC 1 cut(s) 394
Alw26I GTCTC 3 cut(s) 265, 350, 502
Alw44I GTGCAC 1 cut(s) 390
AlwNI CAGNNNCTG 1 cut(s) 331
ApaLI GTGCAC 1 cut(s) 390
ApeKI GCWGC 3 cut(s) 469, 472, 586
ApoI RAATTY 1 cut(s) 346
Asp700I GAANNNNTTC 1 cut(s) 327
AspLEI GCGC 1 cut(s) 304
AsuHPI GGTGA 1 cut(s) 137
BaeGI GKGCMC 1 cut(s) 394
BanI GGYRCC 1 cut(s) 709
Bbv12I GWGCWC 1 cut(s) 394
BbvCI CCTCAGC 1 cut(s) 114
BbvI GCAGC 3 cut(s) 481, 484, 598
BccI CCATC 4 cut(s) 62, 116, 520, 600
BcoDI GTCTC 3 cut(s) 265, 350, 502
BcuI ACTAGT 1 cut(s) 610
BfaI CTAG 4 cut(s) 45, 600, 611, 623
BisI GCNGC 3 cut(s) 470, 473, 587
BlsI GCNGC 3 cut(s) 471, 474, 588
BmiI GGNNCC 1 cut(s) 711
BmsI GCATC 5 cut(s) 38, 81, 85, 229, 574
Bpu10I CCTNAGC 1 cut(s) 114
BpuEI CTTGAG 2 cut(s) 264, 674
BsaAI YACGTR 1 cut(s) 389
BsaHI GRCGYC 1 cut(s) 258
BsaI GGTCTC 1 cut(s) 502
Bse3DI GCAATG 1 cut(s) 581
BseGI GGATG 3 cut(s) 100, 124, 667
BseMI GCAATG 1 cut(s) 581
BseMII CTCAG 2 cut(s) 105, 308
BseSI GKGCMC 1 cut(s) 394
BseXI GCAGC 3 cut(s) 481, 484, 598
Bsh1236I CGCG 2 cut(s) 302, 304
BshNI GGYRCC 1 cut(s) 709
BsiHKAI GWGCWC 1 cut(s) 394
BsmAI GTCTC 3 cut(s) 265, 350, 502
BsmBI CGTCTC 1 cut(s) 265
Bso31I GGTCTC 1 cut(s) 502
Bsp1286I GDGCHC 1 cut(s) 394
Bsp143I GATC 3 cut(s) 19, 297, 685
BspCNI CTCAG 2 cut(s) 106, 307
BspFNI CGCG 2 cut(s) 302, 304
BspLI GGNNCC 1 cut(s) 711
BspQI GCTCTTC 1 cut(s) 330
BspT107I GGYRCC 1 cut(s) 709
BspTNI GGTCTC 1 cut(s) 502
BsrDI GCAATG 1 cut(s) 581
BssMI GATC 3 cut(s) 19, 297, 685
BssNI GRCGYC 1 cut(s) 258
Bst6I CTCTTC 2 cut(s) 330, 348
BstACI GRCGYC 1 cut(s) 258
BstBAI YACGTR 1 cut(s) 389
BstC8I GCNNGC 2 cut(s) 27, 42
BstDEI CTNAG 2 cut(s) 114, 294
BstF5I GGATG 3 cut(s) 100, 124, 667
BstFNI CGCG 2 cut(s) 302, 304
BstHHI GCGC 1 cut(s) 304
BstKTI GATC 3 cut(s) 22, 300, 688
BstMAI GTCTC 3 cut(s) 265, 350, 502
BstMBI GATC 3 cut(s) 19, 297, 685
BstMWI GCNNNNNNNGC 2 cut(s) 62, 248
BstNSI RCATGY 2 cut(s) 398, 727
BstSLI GKGCMC 1 cut(s) 394
BstUI CGCG 2 cut(s) 302, 304
BstV1I GCAGC 3 cut(s) 481, 484, 598
BtsCI GGATG 3 cut(s) 100, 124, 667
Cac8I GCNNGC 2 cut(s) 27, 42
CaiI CAGNNNCTG 1 cut(s) 331
CfoI GCGC 1 cut(s) 304
CspCI CAANNNNNGTGG 2 cut(s) 513, 548
CviAII CATG 3 cut(s) 395, 413, 724
CviJI RGCY 9 cut(s) 44, 56, 113, 160, 340, 406, 589, 599, 653
CviKI_1 RGCY 9 cut(s) 44, 56, 113, 160, 340, 406, 589, 599, 653
DdeI CTNAG 2 cut(s) 114, 294
DpnI GATC 3 cut(s) 21, 299, 687
DpnII GATC 3 cut(s) 19, 297, 685
Eam1104I CTCTTC 2 cut(s) 330, 348
EarI CTCTTC 2 cut(s) 330, 348
Eco31I GGTCTC 1 cut(s) 502
Eco57I CTGAAG 1 cut(s) 20
Esp3I CGTCTC 1 cut(s) 265
FaeI CATG 3 cut(s) 398, 416, 727
FalI AAGNNNNNCTT 6 cut(s) 45, 77, 144, 176, 438, 470
FatI CATG 3 cut(s) 394, 412, 723
FauNDI CATATG 1 cut(s) 222
Fnu4HI GCNGC 3 cut(s) 470, 473, 587
FokI GGATG 3 cut(s) 107, 131, 674
Fsp4HI GCNGC 3 cut(s) 470, 473, 587
FspBI CTAG 4 cut(s) 45, 600, 611, 623
GlaI GCGC 1 cut(s) 303
GluI GCNGC 3 cut(s) 470, 473, 587
HhaI GCGC 1 cut(s) 304
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 3 cut(s) 398, 416, 727
Hin6I GCGC 1 cut(s) 302
HinP1I GCGC 1 cut(s) 302
HindIII AAGCTT 1 cut(s) 54
HinfI GANTC 3 cut(s) 277, 328, 358
HphI GGTGA 1 cut(s) 137
Hpy166II GTNNAC 5 cut(s) 272, 386, 392, 616, 647
Hpy188I TCNGA 4 cut(s) 166, 297, 363, 576
Hpy188III TCNNGA 4 cut(s) 332, 377, 556, 623
Hpy8I GTNNAC 5 cut(s) 272, 386, 392, 616, 647
Hpy99I CGWCG 1 cut(s) 645
HpyCH4IV ACGT 3 cut(s) 258, 388, 643
HpyCH4V TGCA 6 cut(s) 392, 469, 524, 565, 586, 723
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 248
HpyF3I CTNAG 2 cut(s) 114, 294
HpySE526I ACGT 3 cut(s) 258, 388, 643
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 3 cut(s) 398, 416, 727
HspAI GCGC 1 cut(s) 302
Kzo9I GATC 3 cut(s) 19, 297, 685
LguI GCTCTTC 1 cut(s) 330
LmnI GCTCC 1 cut(s) 428
LpnPI CCDG 4 cut(s) 11, 317, 581, 702
Lsp1109I GCAGC 3 cut(s) 481, 484, 598
LweI GCATC 5 cut(s) 38, 81, 85, 229, 574
MaeI CTAG 4 cut(s) 45, 600, 611, 623
MaeII ACGT 3 cut(s) 258, 388, 643
MaeIII GTNAC 1 cut(s) 133
MalI GATC 3 cut(s) 21, 299, 687
MboI GATC 3 cut(s) 19, 297, 685
MfeI CAATTG 1 cut(s) 432
MhlI GDGCHC 1 cut(s) 394
MluCI AATT 3 cut(s) 346, 432, 464
MlyI GAGTC 2 cut(s) 271, 352
MnlI CCTC 4 cut(s) 85, 109, 121, 652
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 1 cut(s) 733
MunI CAATTG 1 cut(s) 432
MvnI CGCG 2 cut(s) 302, 304
MwoI GCNNNNNNNGC 2 cut(s) 62, 248
NdeI CATATG 1 cut(s) 222
NdeII GATC 3 cut(s) 19, 297, 685
NlaIII CATG 3 cut(s) 398, 416, 727
NlaIV GGNNCC 1 cut(s) 711
NspI RCATGY 2 cut(s) 398, 727
PciI ACATGT 1 cut(s) 394
PciSI GCTCTTC 1 cut(s) 330
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 1 cut(s) 328
PkrI GCNGC 3 cut(s) 471, 474, 588
PleI GAGTC 2 cut(s) 271, 352
PpsI GAGTC 2 cut(s) 271, 352
Ppu21I YACGTR 1 cut(s) 389
PscI ACATGT 1 cut(s) 394
PspN4I GGNNCC 1 cut(s) 711
PstNI CAGNNNCTG 1 cut(s) 331
SapI GCTCTTC 1 cut(s) 330
SaqAI TTAA 1 cut(s) 733
SatI GCNGC 3 cut(s) 470, 473, 587
Sau3AI GATC 3 cut(s) 19, 297, 685
SchI GAGTC 2 cut(s) 271, 352
SduI GDGCHC 1 cut(s) 394
SfaNI GCATC 5 cut(s) 38, 81, 85, 229, 574
SmlI CTYRAG 2 cut(s) 279, 689
SmoI CTYRAG 2 cut(s) 279, 689
SpeI ACTAGT 1 cut(s) 610
Sse9I AATT 3 cut(s) 346, 432, 464
SspMI CTAG 4 cut(s) 45, 600, 611, 623
TaiI ACGT 3 cut(s) 261, 391, 646
TasI AATT 3 cut(s) 346, 432, 464
TfiI GAWTC 1 cut(s) 328
Tru1I TTAA 1 cut(s) 733
Tru9I TTAA 1 cut(s) 733
TseI GCWGC 3 cut(s) 469, 472, 586
TspDTI ATGAA 3 cut(s) 239, 324, 620
VneI GTGCAC 1 cut(s) 390
XapI RAATTY 1 cut(s) 346
XbaI TCTAGA 1 cut(s) 622
XceI RCATGY 2 cut(s) 398, 727
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 4 cut(s) 45, 600, 611, 623
ZraI GACGTC 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.