Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0005783.1

Orthogroup ID

OG0001947

Physical Location & Seq

Genomic Coordinates Reverse (-)

22588 .. 27762

Gene Structure 5175 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2871 bp

atggctcctttctctttgtttatttcatttcttctcactaatcttgctgttcttcaccctgctaaagcagagagaaactacagactttgcccaccctactcctgtaatgatgtcagcatctctttccccttcaaatataaggaacatcctcctgaatgcggattgtattctgttaattgtcctgtaggctctcctccgacgatccaactcaaaaatagaggatcctggcatgaatttacaagcatctctccgtcgaatgagatctcgatcaatgacaaagcgcttcagcagcgactgggaagagattcctgcgatgatgaaagcttcgagtatttaagtcttcccgacccttctccaatatctaatgtgtccaccacacacaatctgaccctgttcaaatgcagcaacacgctggacaaaactcctcatagggatcttaaaatctgctgcagcagtgcttcccatagttattattttttttctcccaattatataccaccaaagtgttcagttgttcagctccccgttcttccgggttggaatctttcgaatcaacctcaccctaacctcactgttaacttcacccttcaagtgacggtatccgaaaaatgttctgaatgctatgatataggagacaggtgccagctggcagacaagggagaaactacttgttcacctgcaccaaaaggtatcggacttggagtaccaacacttcttgcagcatgcttctttattctgtggtgttacagacaaaaatgtgcttctttaaacttggtatcatcaaggaacatttcaaattcagacccagaagggcgcagtgtctcctttggtgtttcagtcttcacctacaaagaacttgaggaagcaactaatcattttgatagtgaaaaagaaattggagatggaggttttggaattgtataccatggtaagagcaagcattctatgtttctgctgatttctttttctttaagctacattaggcaagtaacttctgatatagtacaccgagatgtgaagactaccaacattctcctggatgaaaacttctgtgtaaaagttgcggattttgggatctctcgcctcttcccccttaatgccactcatgtctcaactgctccgcaggggacccctggttatgttgatccagagtatcacgaatgctaccagctaactcccaagagtgatgtttatagttttggggttgttctcattgagctaatatcatctttaccagcagttgatataacaaggcatagggatgaaatcaatctgtctaacatagcaataaacaagattcaaaatggcttatactgtgagctggtggattcacgtcttggctttgagtcagaccctggagttaaaaggatgacaattgcagtagcagacttggcttttcaatgtcttcggcaggatagggatgcaaggcctaacatgtgggaggttttggaggctttggagaagaccgaaagcaggaacgatgtaccaaagaatctaaacccagcattctgtgatgcagggatgggagagagtatccagccatctccttcaccagattgtgaccggattcggttgttgaacagcacgaaggcacaatcttcaccaaattccgtgatggaaaaatggtctagcactagctggaaagtttcgatggctctggtggctttgtttacgtactctgttctcagtcatcttctagttcttcactacgtcgaggggatgaagaagtgtccatcctactactgtaatgaaatggtaggctatattcatttccctttcaaaaatagttcacatcctcctgaatgcgggctgtacacagtcgattgctccaaccctttgagtccgaagatccaactgagagagggaggatactggcatgactttgatagcatttctcaaacaaattcggttcatatcaacgacaaagagctagtgaagcccttgccaatagattactgcaatgatgaaattctcgatgatttgagtcttcccagcccttcttcactctttgatctattgttcaccaacaatctgaccctgttcaaatgcaacacctccaaggggacagcgcctactttggtgtttcggtctttgcctatagtgaacttgaagaagccactaatcattttgatagcaaaaaggaacttggagatggggggttctggaactgtataccatggcaagctcaaagatggccgagaagttgcagtcaagcgcttacacgagcacaactacaaacgtgaggaactgtttatgaatgaaattgaaattatcacccgcctgcactacaaaaatttagtcaccctttatggctgcacttcacgccgcagccatgagctcctccttgtgtatgaatacattcccaatggcactgtttctgatcatctccatggtgacctagcaaaccgtggtttactcacttggcctattcgtataagcattgccatagaaactgccaatgcattgtcttacctccatgcttctgatatcctgcatcgtgatgtgaagactagcaacattctccttgatatcaatttgtgtgtaaaagttgcagattttgggctctcctggattttcccgcttgacatcactcatgtctcaactgctccacaagggacccctggttatgttgacccacagtatcatcaatgttaccagctaactagtaagagtgatgtttatagttttggggttgtactcattgagcttataacgtctatgccagcagttgatataactaggcagcgggatgagatcaatttatctaacttagccgtaaacaagattcgaaaaggattatacaatgagctgttagattcatctcttgggtttgagtcagataatgaagttaaaaggatgacaattgcgtag

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

GUB_WAK_bind Pkinase Pkinase_Tyr WAK_assoc

Protein Analysis

956

Amino Acids

107.11

Weight (kDa)

6.04

Isoelectric Point (pI)

43.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GUB_WAK_bind	PF13947	30 - 91	6.1e-07	Wall-associated receptor kinase galacturonan-binding
PK_Tyr_Ser-Thr	PF07714	315 - 485	9.1e-19	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	322 - 484	1.6e-21	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	712 - 913	1.3e-39	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	716 - 917	1.4e-37	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001947)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G25390 AT1G25390
fragaria_vesca	FvH4_4g26830 FvH4_4g26840 FvH4_4g26841 FvH4_4g26841
malus_domestica	MD13G1036100.v1.1 MD16G1037800.v1.1 MD16G1038800.v1.1
prunus_persica	Prupe.1G315200_v2.0.a1 Prupe.1G315900_v2.0.a1 Prupe.1G315900_v2.0.a1 Prupe.1G315900_v2.0.a1
pyrus_communis	pycom13g03150
rosa_chinensis	RchiOBHm_Chr4g0434491 RchiOBHm_Chr4g0434531 RchiOBHm_Chr4g0434541 RchiOBHm_Chr4g0434651
rosa_laevigata	RLG00000006663 RLG00000006664 RLG00000006665
rosa_multiflora	Rmu_co8505535.1_g000001 Rmu_sc0005783.1_g000005 Rmu_sc0005783.1_g000006 Rmu_sc0005783.1_g000008 Rmu_sc0005899.1_g000015 Rmu_sc0008439.1_g000007
rosa_roxburghii	Rroxscaffold_5G00375850
rosa_rugosa	Rorug04G0277200 Rorug04G0277200 Rorug04G0277200
rosa_samantha	Rh4AG333000 Rh4AG333900 Rh4BG340700 Rh4BG340800 Rh4BG341100 Rh4BG341600 Rh4BG341900 Rh4BG342100 Rh4BG342300 Rh4DG336000
rosa_wichuraiana	Rw4G028860 Rw4G028870 Rw4G028880 Rw4G028890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2711
AarI	CACCTGC	1 cut(s)	685
Acc36I	ACCTGC	1 cut(s)	685
AccB1I	GGYRCC	1 cut(s)	639
AccI	GTMKAC	2 cut(s)	921, 2172
AciI	CCGC	8 cut(s)	159, 1064, 1121, 1806, 2278, 2326, 2579, 2746
AclWI	GGATC	7 cut(s)	196, 216, 229, 441, 1082, 1139, 1843
AcoI	YGGCCR	1 cut(s)	2194
AcsI	RAATTY	6 cut(s)	233, 796, 1606, 1903, 1968, 2293
AcuI	CTGAAG	1 cut(s)	269
AfaI	GTAC	6 cut(s)	705, 1005, 1485, 1676, 1814, 2697
AfeI	AGCGCT	2 cut(s)	282, 2216
AfiI	CCNNNNNNNGG	4 cut(s)	158, 1473, 1805, 2061
AflIII	ACRYGT	1 cut(s)	1434
AhlI	ACTAGT	1 cut(s)	2663
AjiI	CACGTC	1 cut(s)	1334
AjnI	CCWGG	6 cut(s)	224, 1035, 1132, 1354, 2567, 2620
AjuI	GAANNNNNNNTTGG	2 cut(s)	879, 911
AleI	CACNNNNGTG	1 cut(s)	502
Alw21I	GWGCWC	2 cut(s)	2229, 2340
Alw26I	GTCTC	4 cut(s)	627, 826, 1114, 2602
AlwI	GGATC	7 cut(s)	196, 216, 229, 441, 1082, 1139, 1843
AlwNI	CAGNNNCTG	2 cut(s)	295, 1355
Aor51HI	AGCGCT	2 cut(s)	282, 2216
AoxI	GGCC	3 cut(s)	1427, 2194, 2423
ApeKI	GCWGC	8 cut(s)	289, 402, 447, 450, 719, 2313, 2328, 2743
ApoI	RAATTY	6 cut(s)	233, 796, 1606, 1903, 1968, 2293
ArsI	GACNNNNNNTTYG	2 cut(s)	2283, 2315
Asp700I	GAANNNNTTC	2 cut(s)	304, 2358
AspLEI	GCGC	4 cut(s)	283, 816, 2071, 2217
AspS9I	GGNCC	2 cut(s)	1128, 2616
AsuC2I	CCSGG	1 cut(s)	534
AsuII	TTCGAA	2 cut(s)	548, 2788
AvaII	GGWCC	2 cut(s)	1128, 2616
BamHI	GGATCC	1 cut(s)	221
BanI	GGYRCC	1 cut(s)	639
BanII	GRGCYC	2 cut(s)	2340, 2565
BarI	GAAGNNNNNNTAC	2 cut(s)	696, 728
BauI	CACGAG	1 cut(s)	2222
BbsI	GAAGAC	7 cut(s)	332, 832, 1025, 1397, 1469, 1979, 2513
Bbv12I	GWGCWC	2 cut(s)	2229, 2340
BbvI	GCAGC	8 cut(s)	301, 414, 434, 462, 731, 2300, 2340, 2755
BccI	CCATC	8 cut(s)	896, 1516, 1549, 1609, 1645, 1741, 2146, 2186
BceAI	ACGGC	1 cut(s)	2759
BciT130I	CCWGG	6 cut(s)	226, 1037, 1134, 1356, 2569, 2622
BciVI	GTATCC	3 cut(s)	610, 1544, 1862
BclI	TGATCA	1 cut(s)	2380
BcnI	CCSGG	1 cut(s)	534
BcoDI	GTCTC	4 cut(s)	627, 826, 1114, 2602
BcuI	ACTAGT	1 cut(s)	2663
BfaI	CTAG	8 cut(s)	1629, 1635, 1697, 1931, 2399, 2511, 2664, 2739
BfmI	CTRYAG	4 cut(s)	79, 183, 448, 2097
BfoI	RGCGCY	3 cut(s)	284, 2072, 2218
BfuAI	ACCTGC	1 cut(s)	685
BfuI	GTATCC	3 cut(s)	610, 1544, 1862
BglII	AGATCT	1 cut(s)	261
BisI	GCNGC	9 cut(s)	290, 403, 448, 451, 720, 2314, 2326, 2329, 2744
BlsI	GCNGC	9 cut(s)	291, 404, 449, 452, 721, 2315, 2327, 2330, 2745
Bme1390I	CCNGG	7 cut(s)	226, 534, 1037, 1134, 1356, 2569, 2622
Bme18I	GGWCC	2 cut(s)	1128, 2616
BmgBI	CACGTC	1 cut(s)	1334
BmgT120I	GGNCC	2 cut(s)	1128, 2616
BmiI	GGNNCC	7 cut(s)	6, 223, 641, 1129, 1130, 2617, 2618
BmrFI	CCNGG	7 cut(s)	226, 534, 1037, 1134, 1356, 2569, 2622
BmrI	ACTGGG	1 cut(s)	305
BmsI	GCATC	5 cut(s)	126, 252, 1411, 1504, 2503
BmuI	ACTGGG	1 cut(s)	305
BpiI	GAAGAC	7 cut(s)	332, 832, 1025, 1397, 1469, 1979, 2513
BpmI	CTGGAG	1 cut(s)	1377
Bpu14I	TTCGAA	2 cut(s)	548, 2788
BpuEI	CTTGAG	1 cut(s)	878
BpuMI	CCSGG	1 cut(s)	534
BsaAI	YACGTR	1 cut(s)	1674
BsaBI	GATNNNNATC	1 cut(s)	266
BsaJI	CCNNGG	8 cut(s)	925, 1132, 1354, 2058, 2176, 2389, 2407, 2620
BsaWI	WCCGGW	1 cut(s)	1563
Bsc4I	CCNNNNNNNGG	4 cut(s)	158, 1473, 1805, 2061
Bse1I	ACTGG	2 cut(s)	300, 1877
Bse3DI	GCAATG	2 cut(s)	1966, 2439
Bse8I	GATNNNNATC	1 cut(s)	266
BseBI	CCWGG	6 cut(s)	226, 1037, 1134, 1356, 2569, 2622
BseDI	CCNNGG	8 cut(s)	925, 1132, 1354, 2058, 2176, 2389, 2407, 2620
BseJI	GATNNNNATC	1 cut(s)	266
BseLI	CCNNNNNNNGG	4 cut(s)	158, 1473, 1805, 2061
BseMI	GCAATG	2 cut(s)	1966, 2439
BseMII	CTCAG	2 cut(s)	1699, 1847
BseNI	ACTGG	2 cut(s)	300, 1877
BseRI	GAGGAG	3 cut(s)	183, 414, 2330
BseXI	GCAGC	8 cut(s)	301, 414, 434, 462, 731, 2300, 2340, 2755
BseYI	CCCAGC	2 cut(s)	1501, 1991
BsgI	GTGCAG	3 cut(s)	663, 2267, 2299
BshFI	GGCC	3 cut(s)	1429, 2196, 2425
BshNI	GGYRCC	1 cut(s)	639
BsiHKAI	GWGCWC	2 cut(s)	2229, 2340
BsiSI	CCGG	2 cut(s)	533, 1564
BslFI	GGGAC	3 cut(s)	1141, 2077, 2629
BslI	CCNNNNNNNGG	4 cut(s)	158, 1473, 1805, 2061
BsmAI	GTCTC	4 cut(s)	627, 826, 1114, 2602
BsmFI	GGGAC	3 cut(s)	1141, 2077, 2629
BsmI	GAATGC	6 cut(s)	161, 623, 940, 1166, 1505, 1808
BsnI	GGCC	3 cut(s)	1429, 2196, 2425
Bsp119I	TTCGAA	2 cut(s)	548, 2788
Bsp1286I	GDGCHC	3 cut(s)	2229, 2340, 2565
Bsp1407I	TGTACA	1 cut(s)	1812
Bsp19I	CCATGG	3 cut(s)	925, 2176, 2389
BspACI	CCGC	8 cut(s)	159, 1064, 1121, 1806, 2278, 2326, 2579, 2746
BspANI	GGCC	3 cut(s)	1429, 2196, 2425
BspCNI	CTCAG	2 cut(s)	1698, 1848
BspLI	GGNNCC	7 cut(s)	6, 223, 641, 1129, 1130, 2617, 2618
BspMAI	CTGCAG	1 cut(s)	452
BspMI	ACCTGC	1 cut(s)	685
BspPI	GGATC	7 cut(s)	196, 216, 229, 441, 1082, 1139, 1843
BspT104I	TTCGAA	2 cut(s)	548, 2788
BspT107I	GGYRCC	1 cut(s)	639
BsrDI	GCAATG	2 cut(s)	1966, 2439
BsrGI	TGTACA	1 cut(s)	1812
BsrI	ACTGG	2 cut(s)	300, 1877
BssECI	CCNNGG	8 cut(s)	925, 1132, 1354, 2058, 2176, 2389, 2407, 2620
BssNAI	GTATAC	2 cut(s)	922, 2173
BssSI	CACGAG	1 cut(s)	2222
BssT1I	CCWWGG	4 cut(s)	925, 2058, 2176, 2389
Bst1107I	GTATAC	2 cut(s)	922, 2173
Bst2BI	CACGAG	1 cut(s)	2222
Bst2UI	CCWGG	6 cut(s)	226, 1037, 1134, 1356, 2569, 2622
Bst6I	CTCTTC	2 cut(s)	295, 1091
BstAUI	TGTACA	1 cut(s)	1812
BstBAI	YACGTR	1 cut(s)	1674
BstBI	TTCGAA	2 cut(s)	548, 2788
BstC8I	GCNNGC	8 cut(s)	644, 648, 724, 938, 1808, 2183, 2282, 2724
BstDEI	CTNAG	3 cut(s)	1685, 1856, 2770
BstDSI	CCRYGG	4 cut(s)	925, 2176, 2389, 2407
BstEII	GGTNACC	1 cut(s)	2393
BstH2I	RGCGCY	3 cut(s)	284, 2072, 2218
BstHHI	GCGC	4 cut(s)	283, 816, 2071, 2217
BstMAI	GTCTC	4 cut(s)	627, 826, 1114, 2602
BstMWI	GCNNNNNNNGC	5 cut(s)	289, 1391, 1661, 1936, 2322
BstNI	CCWGG	6 cut(s)	226, 1037, 1134, 1356, 2569, 2622
BstNSI	RCATGY	2 cut(s)	726, 1438
BstPI	GGTNACC	1 cut(s)	2393
BstSCI	CCNGG	7 cut(s)	224, 532, 1035, 1132, 1354, 2567, 2620
BstSFI	CTRYAG	4 cut(s)	79, 183, 448, 2097
BstSNI	TACGTA	1 cut(s)	1674
BstV1I	GCAGC	8 cut(s)	301, 414, 434, 462, 731, 2300, 2340, 2755
BstV2I	GAAGAC	7 cut(s)	332, 832, 1025, 1397, 1469, 1979, 2513
BstX2I	RGATCY	5 cut(s)	221, 261, 433, 1074, 1848
BstYI	RGATCY	5 cut(s)	221, 261, 433, 1074, 1848
BstZ17I	GTATAC	2 cut(s)	922, 2173
BsuI	GTATCC	3 cut(s)	610, 1544, 1862
BsuRI	GGCC	3 cut(s)	1429, 2196, 2425
BtgI	CCRYGG	4 cut(s)	925, 2176, 2389, 2407
BtgZI	GCGATG	1 cut(s)	327
BtrI	CACGTC	1 cut(s)	1334
BtsI	GCAGTG	2 cut(s)	460, 823
BtsIMutI	CAGTG	4 cut(s)	460, 570, 823, 2370
BveI	ACCTGC	1 cut(s)	685
Cac8I	GCNNGC	8 cut(s)	644, 648, 724, 938, 1808, 2183, 2282, 2724
CaiI	CAGNNNCTG	2 cut(s)	295, 1355
CfoI	GCGC	4 cut(s)	283, 816, 2071, 2217
Cfr13I	GGNCC	2 cut(s)	1128, 2616
Csp6I	GTAC	6 cut(s)	704, 1004, 1484, 1675, 1813, 2696
CviQI	GTAC	6 cut(s)	704, 1004, 1484, 1675, 1813, 2696
DdeI	CTNAG	3 cut(s)	1685, 1856, 2770
DraI	TTTAAA	1 cut(s)	768
EaeI	YGGCCR	1 cut(s)	2194
Eam1104I	CTCTTC	2 cut(s)	295, 1091
EarI	CTCTTC	2 cut(s)	295, 1091
Ecl136II	GAGCTC	1 cut(s)	2338
Eco105I	TACGTA	1 cut(s)	1674
Eco130I	CCWWGG	4 cut(s)	925, 2058, 2176, 2389
Eco147I	AGGCCT	1 cut(s)	1429
Eco24I	GRGCYC	2 cut(s)	2340, 2565
Eco32I	GATATC	2 cut(s)	2488, 2530
Eco47I	GGWCC	2 cut(s)	1128, 2616
Eco47III	AGCGCT	2 cut(s)	282, 2216
Eco53kI	GAGCTC	1 cut(s)	2338
Eco57I	CTGAAG	1 cut(s)	269
Eco91I	GGTNACC	1 cut(s)	2393
EcoICRI	GAGCTC	1 cut(s)	2338
EcoO109I	RGGNCCY	2 cut(s)	1128, 2616
EcoO65I	GGTNACC	1 cut(s)	2393
EcoRII	CCWGG	6 cut(s)	224, 1035, 1132, 1354, 2567, 2620
EcoRV	GATATC	2 cut(s)	2488, 2530
EcoT14I	CCWWGG	4 cut(s)	925, 2058, 2176, 2389
EcoT22I	ATGCAT	1 cut(s)	2464
EcoT38I	GRGCYC	2 cut(s)	2340, 2565
ErhI	CCWWGG	4 cut(s)	925, 2058, 2176, 2389
FaqI	GGGAC	3 cut(s)	1141, 2077, 2629
FauI	CCCGC	4 cut(s)	1799, 2285, 2586, 2739
FbaI	TGATCA	1 cut(s)	2380
FblI	GTMKAC	2 cut(s)	921, 2172
Fnu4HI	GCNGC	9 cut(s)	290, 403, 448, 451, 720, 2314, 2326, 2329, 2744
FriOI	GRGCYC	2 cut(s)	2340, 2565
Fsp4HI	GCNGC	9 cut(s)	290, 403, 448, 451, 720, 2314, 2326, 2329, 2744
FspBI	CTAG	8 cut(s)	1629, 1635, 1697, 1931, 2399, 2511, 2664, 2739
GlaI	GCGC	4 cut(s)	282, 815, 2070, 2216
GluI	GCNGC	9 cut(s)	290, 403, 448, 451, 720, 2314, 2326, 2329, 2744
GsaI	CCCAGC	2 cut(s)	1505, 1995
GsuI	CTGGAG	1 cut(s)	1377
HaeII	RGCGCY	3 cut(s)	284, 2072, 2218
HaeIII	GGCC	3 cut(s)	1429, 2196, 2425
HapII	CCGG	2 cut(s)	533, 1564
HhaI	GCGC	4 cut(s)	283, 816, 2071, 2217
Hin6I	GCGC	4 cut(s)	281, 814, 2069, 2215
HinP1I	GCGC	4 cut(s)	281, 814, 2069, 2215
HincII	GTYRAC	2 cut(s)	577, 2632
HindII	GTYRAC	2 cut(s)	577, 2632
HindIII	AAGCTT	1 cut(s)	322
HpaI	GTTAAC	1 cut(s)	577
HpaII	CCGG	2 cut(s)	533, 1564
Hpy188III	TCNNGA	9 cut(s)	152, 265, 344, 1148, 1157, 1799, 1973, 2163, 2498
Hpy99I	CGWCG	3 cut(s)	202, 256, 1715
HpyAV	CCTTC	7 cut(s)	139, 360, 596, 803, 1557, 1582, 2007
HpyCH4IV	ACGT	5 cut(s)	1333, 1673, 1710, 2239, 2714
HpyF10VI	GCNNNNNNNGC	5 cut(s)	289, 1391, 1661, 1936, 2322
HpyF3I	CTNAG	3 cut(s)	1685, 1856, 2770
HpySE526I	ACGT	5 cut(s)	1333, 1673, 1710, 2239, 2714
HspAI	GCGC	4 cut(s)	281, 814, 2069, 2215
KflI	GGGWCCC	2 cut(s)	1128, 2616
Ksp22I	TGATCA	1 cut(s)	2380
KspAI	GTTAAC	1 cut(s)	577
LmnI	GCTCC	6 cut(s)	10, 525, 1123, 1832, 2343, 2611
Lsp1109I	GCAGC	8 cut(s)	301, 414, 434, 462, 731, 2300, 2340, 2755
LweI	GCATC	5 cut(s)	126, 252, 1411, 1504, 2503
MaeI	CTAG	8 cut(s)	1629, 1635, 1697, 1931, 2399, 2511, 2664, 2739
MaeII	ACGT	5 cut(s)	1333, 1673, 1710, 2239, 2714
MaeIII	GTNAC	7 cut(s)	592, 743, 988, 1559, 2299, 2393, 2651
MfeI	CAATTG	2 cut(s)	1374, 2862
MflI	RGATCY	5 cut(s)	221, 261, 433, 1074, 1848
MhlI	GDGCHC	3 cut(s)	2229, 2340, 2565
MlyI	GAGTC	4 cut(s)	1355, 1849, 1993, 2843
MmeI	TCCRAC	5 cut(s)	221, 229, 518, 1854, 1876
Mph1103I	ATGCAT	1 cut(s)	2464
MroXI	GAANNNNTTC	2 cut(s)	304, 2358
MseI	TTAA	9 cut(s)	174, 335, 438, 576, 767, 971, 1095, 1362, 2850
MslI	CAYNNNNRTG	5 cut(s)	502, 1011, 1260, 2388, 2499
MspA1I	CMGCKG	2 cut(s)	646, 2746
MspI	CCGG	2 cut(s)	533, 1564
MspR9I	CCNGG	7 cut(s)	226, 534, 1037, 1134, 1356, 2569, 2622
MunI	CAATTG	2 cut(s)	1374, 2862
Mva1269I	GAATGC	6 cut(s)	161, 623, 940, 1166, 1505, 1808
MvaI	CCWGG	6 cut(s)	226, 1037, 1134, 1356, 2569, 2622
MwoI	GCNNNNNNNGC	5 cut(s)	289, 1391, 1661, 1936, 2322
NciI	CCSGG	1 cut(s)	534
NcoI	CCATGG	3 cut(s)	925, 2176, 2389
NlaIV	GGNNCC	7 cut(s)	6, 223, 641, 1129, 1130, 2617, 2618
NmeAIII	GCCGAG	1 cut(s)	2222
NmuCI	GTSAC	4 cut(s)	592, 1559, 2299, 2393
NsiI	ATGCAT	1 cut(s)	2464
NspI	RCATGY	2 cut(s)	726, 1438
NspV	TTCGAA	2 cut(s)	548, 2788
OliI	CACNNNNGTG	1 cut(s)	502
PaeI	GCATGC	1 cut(s)	726
PaqCI	CACCTGC	1 cut(s)	685
PceI	AGGCCT	1 cut(s)	1429
PciI	ACATGT	1 cut(s)	1434
PctI	GAATGC	6 cut(s)	161, 623, 940, 1166, 1505, 1808
PdmI	GAANNNNTTC	2 cut(s)	304, 2358
PfeI	GAWTC	9 cut(s)	305, 541, 550, 1297, 1328, 1492, 1567, 2785, 2816
PfoI	TCCNGGA	2 cut(s)	1035, 2567
PkrI	GCNGC	9 cut(s)	291, 404, 449, 452, 721, 2315, 2327, 2330, 2745
PleI	GAGTC	4 cut(s)	1354, 1848, 1992, 2842
PpsI	GAGTC	4 cut(s)	1354, 1848, 1992, 2842
Ppu21I	YACGTR	1 cut(s)	1674
PpuMI	RGGWCCY	2 cut(s)	1128, 2616
PscI	ACATGT	1 cut(s)	1434
PsiI	TTATAA	1 cut(s)	2711
Psp124BI	GAGCTC	1 cut(s)	2340
Psp5II	RGGWCCY	2 cut(s)	1128, 2616
Psp6I	CCWGG	6 cut(s)	224, 1035, 1132, 1354, 2567, 2620
PspEI	GGTNACC	1 cut(s)	2393
PspFI	CCCAGC	2 cut(s)	1501, 1991
PspGI	CCWGG	6 cut(s)	224, 1035, 1132, 1354, 2567, 2620
PspN4I	GGNNCC	7 cut(s)	6, 223, 641, 1129, 1130, 2617, 2618
PspPI	GGNCC	2 cut(s)	1128, 2616
PspPPI	RGGWCCY	2 cut(s)	1128, 2616
PstI	CTGCAG	1 cut(s)	452
PstNI	CAGNNNCTG	2 cut(s)	295, 1355
PsuI	RGATCY	5 cut(s)	221, 261, 433, 1074, 1848
PvuII	CAGCTG	1 cut(s)	646
RsaI	GTAC	6 cut(s)	705, 1005, 1485, 1676, 1814, 2697
RsaNI	GTAC	6 cut(s)	704, 1004, 1484, 1675, 1813, 2696
RseI	CAYNNNNRTG	5 cut(s)	502, 1011, 1260, 2388, 2499
SacI	GAGCTC	1 cut(s)	2340
SaqAI	TTAA	9 cut(s)	174, 335, 438, 576, 767, 971, 1095, 1362, 2850
SatI	GCNGC	9 cut(s)	290, 403, 448, 451, 720, 2314, 2326, 2329, 2744
Sau96I	GGNCC	2 cut(s)	1128, 2616
SchI	GAGTC	4 cut(s)	1355, 1849, 1993, 2843
ScrFI	CCNGG	7 cut(s)	226, 534, 1037, 1134, 1356, 2569, 2622
SduI	GDGCHC	3 cut(s)	2229, 2340, 2565
SfaNI	GCATC	5 cut(s)	126, 252, 1411, 1504, 2503
SfcI	CTRYAG	4 cut(s)	79, 183, 448, 2097
SfuI	TTCGAA	2 cut(s)	548, 2788
SinI	GGWCC	2 cut(s)	1128, 2616
SmiMI	CAYNNNNRTG	5 cut(s)	502, 1011, 1260, 2388, 2499
SmlI	CTYRAG	1 cut(s)	857
SmoI	CTYRAG	1 cut(s)	857
SnaBI	TACGTA	1 cut(s)	1674
SpeI	ACTAGT	1 cut(s)	2663
SphI	GCATGC	1 cut(s)	726
SseBI	AGGCCT	1 cut(s)	1429
SsiI	CCGC	8 cut(s)	159, 1064, 1121, 1806, 2278, 2326, 2579, 2746
SspMI	CTAG	8 cut(s)	1629, 1635, 1697, 1931, 2399, 2511, 2664, 2739
SstI	GAGCTC	1 cut(s)	2340
StuI	AGGCCT	1 cut(s)	1429
StyD4I	CCNGG	7 cut(s)	224, 532, 1035, 1132, 1354, 2567, 2620
StyI	CCWWGG	4 cut(s)	925, 2058, 2176, 2389
TaiI	ACGT	5 cut(s)	1336, 1676, 1713, 2242, 2717
TaqI	TCGA	9 cut(s)	254, 266, 327, 548, 1649, 1713, 1821, 1974, 2788
TaqII	GACCGA	2 cut(s)	1481, 2076
TatI	WGTACW	3 cut(s)	1003, 1812, 2695
TauI	GCSGC	1 cut(s)	2328
TfiI	GAWTC	9 cut(s)	305, 541, 550, 1297, 1328, 1492, 1567, 2785, 2816
Tru1I	TTAA	9 cut(s)	174, 335, 438, 576, 767, 971, 1095, 1362, 2850
Tru9I	TTAA	9 cut(s)	174, 335, 438, 576, 767, 971, 1095, 1362, 2850
TscAI	CASTG	4 cut(s)	460, 577, 823, 2377
TseFI	GTSAC	4 cut(s)	592, 1559, 2299, 2393
TseI	GCWGC	8 cut(s)	289, 402, 447, 450, 719, 2313, 2328, 2743
Tsp45I	GTSAC	4 cut(s)	592, 1559, 2299, 2393
TspGWI	ACGGA	2 cut(s)	240, 1600
TspRI	CASTG	4 cut(s)	460, 577, 823, 2377
VpaK11BI	GGWCC	2 cut(s)	1128, 2616
XapI	RAATTY	6 cut(s)	233, 796, 1606, 1903, 1968, 2293
XceI	RCATGY	2 cut(s)	726, 1438
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1033
XmiI	GTMKAC	2 cut(s)	921, 2172
XmnI	GAANNNNTTC	2 cut(s)	304, 2358
XspI	CTAG	8 cut(s)	1629, 1635, 1697, 1931, 2399, 2511, 2664, 2739
Zsp2I	ATGCAT	1 cut(s)	2464

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.