Rmu_sc0005816.1_g000016

Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005816.1
OG0002770
Physical Location & Seq
Forward (+)
52486 .. 53432
947 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 489 bp
atgactctctacggtagaattcaggctattcaggaagaatcgcaacaattctccaatcctaacgaagtagttcgtctagaatccaatgaacaacaaggcaaccaagttgctgaaaaattggcttatgttagtgcttctaatactaatgtttcttggtgggatactcaagtgctcaggctgcctattgccgaaatttgtcccgtcacacgaaatttcggcacacacaaccacgccgacgcctccactctaatcctgccggcgctgtcgatcagaaatgtgggccagctcgccgtggatctcttagtcgcgtcgacgaaggctgagagagtcgggtttctggatagtccgtatgtccttccatgtgttggcaacgatgcatatggccctgttcctcaaggcccccttgctcttcctcttgaagcttatgaatcgtcttcttatggaatggctttaatccagcaaaggtcacccgttgttaaggtatactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAC2

Protein Analysis

162

Amino Acids

17.72

Weight (kDa)

4.92

Isoelectric Point (pI)

38.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAC2 PF09754 83 - 160 1.2e-09 PAC2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 365
AccI GTMKAC 2 cut(s) 311, 483
AccII CGCG 1 cut(s) 308
AclWI GGATC 1 cut(s) 303
AcsI RAATTY 3 cut(s) 18, 192, 211
AcyI GRCGYC 1 cut(s) 237
AfiI CCNNNNNNNGG 1 cut(s) 365
AgsI TTSAA 1 cut(s) 419
AluBI AGCT 2 cut(s) 286, 422
AluI AGCT 2 cut(s) 286, 422
Alw21I GWGCWC 1 cut(s) 174
AlwI GGATC 1 cut(s) 303
AoxI GGCC 3 cut(s) 280, 382, 397
ApeKI GCWGC 1 cut(s) 178
ApoI RAATTY 3 cut(s) 18, 192, 211
Asp700I GAANNNNTTC 1 cut(s) 69
AspLEI GCGC 1 cut(s) 262
AspS9I GGNCC 3 cut(s) 280, 383, 398
AsuHPI GGTGA 1 cut(s) 459
BaeI ACNNNNGTAYC 2 cut(s) 153, 186
BbsI GAAGAC 1 cut(s) 426
Bbv12I GWGCWC 1 cut(s) 174
BbvI GCAGC 1 cut(s) 165
BceAI ACGGC 1 cut(s) 275
BciVI GTATCC 1 cut(s) 154
BfaI CTAG 2 cut(s) 77, 487
BfoI RGCGCY 1 cut(s) 263
BfuI GTATCC 1 cut(s) 154
BisI GCNGC 1 cut(s) 179
BlsI GCNGC 1 cut(s) 180
BmgT120I GGNCC 3 cut(s) 280, 383, 398
BmiI GGNNCC 1 cut(s) 400
BmsI GCATC 1 cut(s) 364
BpiI GAAGAC 1 cut(s) 426
Bpu10I CCTNAGC 1 cut(s) 173
BpuEI CTTGAG 2 cut(s) 150, 378
BsaHI GRCGYC 1 cut(s) 237
BsaJI CCNNGG 1 cut(s) 291
Bsc4I CCNNNNNNNGG 1 cut(s) 365
Bse118I RCCGGY 1 cut(s) 256
BseDI CCNNGG 1 cut(s) 291
BseLI CCNNNNNNNGG 1 cut(s) 365
BseMII CTCAG 2 cut(s) 187, 312
BseXI GCAGC 1 cut(s) 165
Bsh1236I CGCG 1 cut(s) 308
BshFI GGCC 3 cut(s) 282, 384, 399
BsiHKAI GWGCWC 1 cut(s) 174
BsiSI CCGG 1 cut(s) 257
BslFI GGGAC 1 cut(s) 183
BslI CCNNNNNNNGG 1 cut(s) 365
BsmFI GGGAC 1 cut(s) 183
BsnI GGCC 3 cut(s) 282, 384, 399
Bsp1286I GDGCHC 1 cut(s) 174
Bsp143I GATC 2 cut(s) 267, 295
BspANI GGCC 3 cut(s) 282, 384, 399
BspCNI CTCAG 2 cut(s) 186, 313
BspFNI CGCG 1 cut(s) 308
BspLI GGNNCC 1 cut(s) 400
BspPI GGATC 1 cut(s) 303
BspQI GCTCTTC 1 cut(s) 414
BsrFI RCCGGY 1 cut(s) 256
BssAI RCCGGY 1 cut(s) 256
BssECI CCNNGG 1 cut(s) 291
BssMI GATC 2 cut(s) 267, 295
BssNAI GTATAC 1 cut(s) 484
BssNI GRCGYC 1 cut(s) 237
Bst1107I GTATAC 1 cut(s) 484
Bst4CI ACNGT 1 cut(s) 14
Bst6I CTCTTC 1 cut(s) 414
BstACI GRCGYC 1 cut(s) 237
BstC8I GCNNGC 3 cut(s) 258, 284, 288
BstDEI CTNAG 3 cut(s) 173, 301, 321
BstDSI CCRYGG 1 cut(s) 291
BstEII GGTNACC 1 cut(s) 465
BstFNI CGCG 1 cut(s) 308
BstH2I RGCGCY 1 cut(s) 263
BstHHI GCGC 1 cut(s) 262
BstKTI GATC 2 cut(s) 270, 298
BstMBI GATC 2 cut(s) 267, 295
BstMWI GCNNNNNNNGC 1 cut(s) 178
BstPI GGTNACC 1 cut(s) 465
BstUI CGCG 1 cut(s) 308
BstV1I GCAGC 1 cut(s) 165
BstV2I GAAGAC 1 cut(s) 426
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
BstZ17I GTATAC 1 cut(s) 484
BsuI GTATCC 1 cut(s) 154
BsuRI GGCC 3 cut(s) 282, 384, 399
BtgI CCRYGG 1 cut(s) 291
Cac8I GCNNGC 3 cut(s) 258, 284, 288
CfoI GCGC 1 cut(s) 262
Cfr10I RCCGGY 1 cut(s) 256
Cfr13I GGNCC 3 cut(s) 280, 383, 398
CseI GACGC 2 cut(s) 245, 297
CviAII CATG 1 cut(s) 360
DdeI CTNAG 3 cut(s) 173, 301, 321
DpnI GATC 2 cut(s) 269, 297
DpnII GATC 2 cut(s) 267, 295
Eam1104I CTCTTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 414
Eco91I GGTNACC 1 cut(s) 465
EcoO109I RGGNCCY 1 cut(s) 398
EcoO65I GGTNACC 1 cut(s) 465
EcoRI GAATTC 1 cut(s) 18
EcoT22I ATGCAT 1 cut(s) 379
FaeI CATG 1 cut(s) 363
FaiI YATR 8 cut(s) 126, 351, 361, 379, 381, 426, 441, 484
FalI AAGNNNNNCTT 2 cut(s) 387, 419
FaqI GGGAC 1 cut(s) 183
FatI CATG 1 cut(s) 359
FauNDI CATATG 1 cut(s) 379
FblI GTMKAC 2 cut(s) 311, 483
Fnu4HI GCNGC 1 cut(s) 179
Fsp4HI GCNGC 1 cut(s) 179
FspBI CTAG 2 cut(s) 77, 487
GlaI GCGC 1 cut(s) 261
GluI GCNGC 1 cut(s) 179
HaeII RGCGCY 1 cut(s) 263
HaeIII GGCC 3 cut(s) 282, 384, 399
HapII CCGG 1 cut(s) 257
HgaI GACGC 2 cut(s) 245, 297
HhaI GCGC 1 cut(s) 262
Hin1I GRCGYC 1 cut(s) 237
Hin1II CATG 1 cut(s) 363
Hin6I GCGC 1 cut(s) 260
HinP1I GCGC 1 cut(s) 260
HincII GTYRAC 1 cut(s) 312
HindII GTYRAC 1 cut(s) 312
HindIII AAGCTT 1 cut(s) 420
HinfI GANTC 5 cut(s) 4, 38, 80, 327, 428
HpaII CCGG 1 cut(s) 257
HphI GGTGA 1 cut(s) 459
Hpy166II GTNNAC 2 cut(s) 312, 484
Hpy188I TCNGA 1 cut(s) 272
Hpy188III TCNNGA 4 cut(s) 32, 77, 338, 416
Hpy8I GTNNAC 2 cut(s) 312, 484
Hpy99I CGWCG 3 cut(s) 239, 313, 316
HpyAV CCTTC 2 cut(s) 310, 365
HpyCH4III ACNGT 1 cut(s) 14
HpyCH4V TGCA 1 cut(s) 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 178
HpyF3I CTNAG 3 cut(s) 173, 301, 321
Hsp92I GRCGYC 1 cut(s) 237
Hsp92II CATG 1 cut(s) 363
HspAI GCGC 1 cut(s) 260
KroI GCCGGC 1 cut(s) 256
KroNI GCCGGC 1 cut(s) 258
Kzo9I GATC 2 cut(s) 267, 295
LguI GCTCTTC 1 cut(s) 414
LpnPI CCDG 9 cut(s) 8, 17, 160, 266, 270, 296, 323, 399, 470
Lsp1109I GCAGC 1 cut(s) 165
LweI GCATC 1 cut(s) 364
MaeI CTAG 2 cut(s) 77, 487
MaeIII GTNAC 2 cut(s) 202, 465
MalI GATC 2 cut(s) 269, 297
MboI GATC 2 cut(s) 267, 295
MboII GAAGA 3 cut(s) 47, 401, 426
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 1 cut(s) 174
MluCI AATT 5 cut(s) 18, 47, 116, 192, 211
MlyI GAGTC 1 cut(s) 336
MnlI CCTC 3 cut(s) 250, 402, 423
Mph1103I ATGCAT 1 cut(s) 379
MroNI GCCGGC 1 cut(s) 256
MroXI GAANNNNTTC 1 cut(s) 69
MseI TTAA 2 cut(s) 452, 477
MspI CCGG 1 cut(s) 257
MvnI CGCG 1 cut(s) 308
MwoI GCNNNNNNNGC 1 cut(s) 178
NaeI GCCGGC 1 cut(s) 258
NdeI CATATG 1 cut(s) 379
NdeII GATC 2 cut(s) 267, 295
NgoMIV GCCGGC 1 cut(s) 256
NlaIII CATG 1 cut(s) 363
NlaIV GGNNCC 1 cut(s) 400
NmuCI GTSAC 2 cut(s) 202, 465
NsiI ATGCAT 1 cut(s) 379
PciSI GCTCTTC 1 cut(s) 414
PdiI GCCGGC 1 cut(s) 258
PdmI GAANNNNTTC 1 cut(s) 69
PfeI GAWTC 3 cut(s) 38, 80, 428
PflMI CCANNNNNTGG 1 cut(s) 365
PkrI GCNGC 1 cut(s) 180
PleI GAGTC 1 cut(s) 335
PpsI GAGTC 1 cut(s) 335
PspEI GGTNACC 1 cut(s) 465
PspN4I GGNNCC 1 cut(s) 400
PspPI GGNCC 3 cut(s) 280, 383, 398
PsuI RGATCY 1 cut(s) 295
SalI GTCGAC 1 cut(s) 310
SapI GCTCTTC 1 cut(s) 414
SaqAI TTAA 2 cut(s) 452, 477
SatI GCNGC 1 cut(s) 179
Sau3AI GATC 2 cut(s) 267, 295
Sau96I GGNCC 3 cut(s) 280, 383, 398
SchI GAGTC 1 cut(s) 336
SduI GDGCHC 1 cut(s) 174
SetI ASST 4 cut(s) 288, 424, 467, 483
SfaNI GCATC 1 cut(s) 364
SgrDI CGTCGACG 1 cut(s) 310
SmlI CTYRAG 2 cut(s) 165, 393
SmoI CTYRAG 2 cut(s) 165, 393
Sse9I AATT 5 cut(s) 18, 47, 116, 192, 211
SspMI CTAG 2 cut(s) 77, 487
TaaI ACNGT 1 cut(s) 14
TaqI TCGA 2 cut(s) 266, 311
TasI AATT 5 cut(s) 18, 47, 116, 192, 211
TfiI GAWTC 3 cut(s) 38, 80, 428
Tru1I TTAA 2 cut(s) 452, 477
Tru9I TTAA 2 cut(s) 452, 477
TseFI GTSAC 2 cut(s) 202, 465
TseI GCWGC 1 cut(s) 178
Tsp45I GTSAC 2 cut(s) 202, 465
TspDTI ATGAA 2 cut(s) 102, 441
TspGWI ACGGA 1 cut(s) 336
Van91I CCANNNNNTGG 1 cut(s) 365
XapI RAATTY 3 cut(s) 18, 192, 211
XbaI TCTAGA 1 cut(s) 76
XmiI GTMKAC 2 cut(s) 311, 483
XmnI GAANNNNTTC 1 cut(s) 69
XspI CTAG 2 cut(s) 77, 487
Zsp2I ATGCAT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.