Rmu_sc0009386.1_g000005

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009386.1
OG0005558
Physical Location & Seq
Forward (+)
21098 .. 28908
7811 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009386.1_g000005.1.cds

Sequence Viewer

Length: 654 bp
atgtttcgtccggcggcgtgtcttcaaggcgctgtcgtcagacaggtaggtagaatgtctcagtccgccggcaaaggtcaggagacgtgtatggccttagaggagaacgtttgttgggggaggtctcactatctgcaaacgccgagatcgaaggaaggtcggcggacccagaacggctgcattccgtcagtaatggcagttcggtttggacgaccttgcttgtcgggtacattgcaatattactgtgatttgacaacccggcttggtcgtctatgtggtttggtggtgacgtgggtcaaaggtggcgttgtccggccactggcttcaacggcggtcggatggtatggtggttctgcagatgcgatgccacaattccgacgaataatcattgaaagtcgaggtgttaagaagcttcacatcacaagcattgacagcttccagattgcaaaaagcaagaacaacttccaaatgttgattaatcatgcaggtgaggctgcagttcgttcttggctgaagagaactaagaaggttctgaagaggagtgcaaaatcgaatcagaaactggcaaagaaatataagaaaaacaaagacgtcgaagagtatcaagaagaagtcgatgaagatgagaaggaagaggatgatgcccaggactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

217

Amino Acids

24.38

Weight (kDa)

9.62

Isoelectric Point (pI)

62.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 476
AatII GACGTC 1 cut(s) 594
Acc36I ACCTGC 1 cut(s) 476
AciI CCGC 4 cut(s) 14, 66, 163, 332
AclI AACGTT 1 cut(s) 108
AcoI YGGCCR 1 cut(s) 314
AcuI CTGAAG 2 cut(s) 533, 554
AcyI GRCGYC 1 cut(s) 591
AfaI GTAC 1 cut(s) 229
AfiI CCNNNNNNNGG 1 cut(s) 319
AflIII ACRYGT 1 cut(s) 86
AgsI TTSAA 3 cut(s) 26, 327, 392
AjiI CACGTC 2 cut(s) 87, 291
AjnI CCWGG 1 cut(s) 645
AluBI AGCT 2 cut(s) 412, 435
AluI AGCT 2 cut(s) 412, 435
Alw26I GTCTC 3 cut(s) 63, 77, 129
AlwNI CAGNNNCTG 1 cut(s) 562
AoxI GGCC 2 cut(s) 93, 314
ApeKI GCWGC 2 cut(s) 177, 494
AseI ATTAAT 1 cut(s) 477
Asp700I GAANNNNTTC 1 cut(s) 461
AspLEI GCGC 1 cut(s) 32
AspS9I GGNCC 1 cut(s) 165
AsuC2I CCSGG 1 cut(s) 259
AsuHPI GGTGA 2 cut(s) 298, 500
AvaII GGWCC 1 cut(s) 165
BbsI GAAGAC 1 cut(s) 14
BbvI GCAGC 2 cut(s) 164, 481
BccI CCATC 1 cut(s) 333
BceAI ACGGC 2 cut(s) 190, 345
BciT130I CCWGG 1 cut(s) 647
BcnI CCSGG 1 cut(s) 259
BcoDI GTCTC 3 cut(s) 63, 77, 129
BfmI CTRYAG 2 cut(s) 354, 495
BfoI RGCGCY 1 cut(s) 33
BfuAI ACCTGC 1 cut(s) 476
BisI GCNGC 3 cut(s) 15, 178, 495
BlsI GCNGC 3 cut(s) 16, 179, 496
Bme1390I CCNGG 2 cut(s) 259, 647
Bme18I GGWCC 1 cut(s) 165
BmgBI CACGTC 2 cut(s) 87, 291
BmgT120I GGNCC 1 cut(s) 165
BmiI GGNNCC 1 cut(s) 167
BmrFI CCNGG 2 cut(s) 259, 647
BmsI GCATC 3 cut(s) 349, 354, 631
BoxI GACNNNNGTC 1 cut(s) 293
BpiI GAAGAC 1 cut(s) 14
BpuMI CCSGG 1 cut(s) 259
BsaHI GRCGYC 1 cut(s) 591
BsaI GGTCTC 1 cut(s) 129
BsaJI CCNNGG 1 cut(s) 645
Bsc4I CCNNNNNNNGG 1 cut(s) 319
Bse118I RCCGGY 1 cut(s) 68
Bse1I ACTGG 2 cut(s) 324, 567
Bse3DI GCAATG 1 cut(s) 230
BseBI CCWGG 1 cut(s) 647
BseDI CCNNGG 1 cut(s) 645
BseGI GGATG 2 cut(s) 344, 643
BseLI CCNNNNNNNGG 1 cut(s) 319
BseMI GCAATG 1 cut(s) 230
BseMII CTCAG 1 cut(s) 74
BseNI ACTGG 2 cut(s) 324, 567
BseRI GAGGAG 2 cut(s) 116, 553
BseXI GCAGC 2 cut(s) 164, 481
Bsh1285I CGRYCG 1 cut(s) 336
BshFI GGCC 2 cut(s) 95, 316
BsiEI CGRYCG 1 cut(s) 336
BsiSI CCGG 4 cut(s) 11, 69, 259, 313
BslI CCNNNNNNNGG 1 cut(s) 319
BsmAI GTCTC 3 cut(s) 63, 77, 129
BsmBI CGTCTC 1 cut(s) 77
BsmI GAATGC 1 cut(s) 180
BsnI GGCC 2 cut(s) 95, 316
Bso31I GGTCTC 1 cut(s) 129
Bsp143I GATC 1 cut(s) 146
BspACI CCGC 4 cut(s) 14, 66, 163, 332
BspANI GGCC 2 cut(s) 95, 316
BspCNI CTCAG 1 cut(s) 73
BspLI GGNNCC 1 cut(s) 167
BspMAI CTGCAG 2 cut(s) 358, 499
BspMI ACCTGC 1 cut(s) 476
BspTNI GGTCTC 1 cut(s) 129
BsrDI GCAATG 1 cut(s) 230
BsrFI RCCGGY 1 cut(s) 68
BsrI ACTGG 2 cut(s) 324, 567
BssAI RCCGGY 1 cut(s) 68
BssECI CCNNGG 1 cut(s) 645
BssMI GATC 1 cut(s) 146
BssNI GRCGYC 1 cut(s) 591
Bst2UI CCWGG 1 cut(s) 647
Bst4CI ACNGT 1 cut(s) 245
Bst6I CTCTTC 4 cut(s) 509, 530, 591, 627
BstACI GRCGYC 1 cut(s) 591
BstC8I GCNNGC 1 cut(s) 70
BstDEI CTNAG 3 cut(s) 60, 97, 522
BstF5I GGATG 2 cut(s) 344, 643
BstH2I RGCGCY 1 cut(s) 33
BstHHI GCGC 1 cut(s) 32
BstKTI GATC 1 cut(s) 149
BstMAI GTCTC 3 cut(s) 63, 77, 129
BstMBI GATC 1 cut(s) 146
BstMCI CGRYCG 1 cut(s) 336
BstMWI GCNNNNNNNGC 3 cut(s) 329, 432, 491
BstNI CCWGG 1 cut(s) 647
BstPAI GACNNNNGTC 1 cut(s) 293
BstSCI CCNGG 2 cut(s) 257, 645
BstSFI CTRYAG 2 cut(s) 354, 495
BstV1I GCAGC 2 cut(s) 164, 481
BstV2I GAAGAC 1 cut(s) 14
BsuRI GGCC 2 cut(s) 95, 316
BtgZI GCGATG 1 cut(s) 377
BtrI CACGTC 2 cut(s) 87, 291
BtsCI GGATG 2 cut(s) 344, 643
BtsIMutI CAGTG 1 cut(s) 317
BveI ACCTGC 1 cut(s) 476
Cac8I GCNNGC 1 cut(s) 70
CaiI CAGNNNCTG 1 cut(s) 562
CfoI GCGC 1 cut(s) 32
Cfr10I RCCGGY 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 165
Csp6I GTAC 1 cut(s) 228
CviAII CATG 1 cut(s) 482
CviJI RGCY 9 cut(s) 95, 177, 262, 316, 323, 412, 435, 494, 511
CviKI_1 RGCY 9 cut(s) 95, 177, 262, 316, 323, 412, 435, 494, 511
CviQI GTAC 1 cut(s) 228
DdeI CTNAG 3 cut(s) 60, 97, 522
DpnI GATC 1 cut(s) 148
DpnII GATC 1 cut(s) 146
EaeI YGGCCR 1 cut(s) 314
Eam1104I CTCTTC 4 cut(s) 509, 530, 591, 627
EarI CTCTTC 4 cut(s) 509, 530, 591, 627
EciI GGCGGA 2 cut(s) 55, 178
Eco31I GGTCTC 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 165
Eco57I CTGAAG 2 cut(s) 533, 554
EcoRII CCWGG 1 cut(s) 645
Esp3I CGTCTC 1 cut(s) 77
FaeI CATG 1 cut(s) 485
FaiI YATR 5 cut(s) 92, 274, 345, 483, 576
FalI AAGNNNNNCTT 2 cut(s) 446, 478
FatI CATG 1 cut(s) 481
Fnu4HI GCNGC 3 cut(s) 15, 178, 495
FokI GGATG 2 cut(s) 351, 650
Fsp4HI GCNGC 3 cut(s) 15, 178, 495
GlaI GCGC 1 cut(s) 31
GluI GCNGC 3 cut(s) 15, 178, 495
HaeII RGCGCY 1 cut(s) 33
HaeIII GGCC 2 cut(s) 95, 316
HapII CCGG 4 cut(s) 11, 69, 259, 313
HhaI GCGC 1 cut(s) 32
Hin1I GRCGYC 1 cut(s) 591
Hin1II CATG 1 cut(s) 485
Hin6I GCGC 1 cut(s) 30
HinP1I GCGC 1 cut(s) 30
HindIII AAGCTT 1 cut(s) 410
HinfI GANTC 1 cut(s) 553
HpaII CCGG 4 cut(s) 11, 69, 259, 313
HphI GGTGA 2 cut(s) 298, 500
Hpy188I TCNGA 5 cut(s) 41, 338, 377, 534, 558
Hpy188III TCNNGA 3 cut(s) 80, 439, 605
Hpy99I CGWCG 2 cut(s) 381, 596
HpyAV CCTTC 4 cut(s) 145, 149, 520, 622
HpyCH4III ACNGT 1 cut(s) 245
HpyCH4IV ACGT 4 cut(s) 86, 108, 290, 591
HpyCH4V TGCA 8 cut(s) 136, 180, 235, 356, 446, 485, 497, 545
HpyF10VI GCNNNNNNNGC 3 cut(s) 329, 432, 491
HpyF3I CTNAG 3 cut(s) 60, 97, 522
HpySE526I ACGT 4 cut(s) 86, 108, 290, 591
Hsp92I GRCGYC 1 cut(s) 591
Hsp92II CATG 1 cut(s) 485
HspAI GCGC 1 cut(s) 30
KroI GCCGGC 1 cut(s) 68
KroNI GCCGGC 1 cut(s) 70
Kzo9I GATC 1 cut(s) 146
Lsp1109I GCAGC 2 cut(s) 164, 481
LweI GCATC 3 cut(s) 349, 354, 631
MaeII ACGT 4 cut(s) 86, 108, 290, 591
MaeIII GTNAC 1 cut(s) 286
MalI GATC 1 cut(s) 148
MboI GATC 1 cut(s) 146
MboII GAAGA 7 cut(s) 14, 526, 547, 608, 620, 632, 644
MluCI AATT 1 cut(s) 371
MmeI TCCRAC 2 cut(s) 316, 400
MnlI CCTC 6 cut(s) 94, 114, 392, 484, 531, 628
MroNI GCCGGC 1 cut(s) 68
MroXI GAANNNNTTC 1 cut(s) 461
MseI TTAA 2 cut(s) 405, 477
MslI CAYNNNNRTG 1 cut(s) 486
MspI CCGG 4 cut(s) 11, 69, 259, 313
MspR9I CCNGG 2 cut(s) 259, 647
Mva1269I GAATGC 1 cut(s) 180
MvaI CCWGG 1 cut(s) 647
MwoI GCNNNNNNNGC 3 cut(s) 329, 432, 491
NaeI GCCGGC 1 cut(s) 70
NciI CCSGG 1 cut(s) 259
NdeII GATC 1 cut(s) 146
NgoMIV GCCGGC 1 cut(s) 68
NlaIII CATG 1 cut(s) 485
NlaIV GGNNCC 1 cut(s) 167
NmeAIII GCCGAG 1 cut(s) 168
NmuCI GTSAC 1 cut(s) 286
PaqCI CACCTGC 1 cut(s) 476
PcsI WCGNNNNNNNCGW 2 cut(s) 146, 208
PctI GAATGC 1 cut(s) 180
PdiI GCCGGC 1 cut(s) 70
PdmI GAANNNNTTC 1 cut(s) 461
PfeI GAWTC 1 cut(s) 553
PkrI GCNGC 3 cut(s) 16, 179, 496
PshAI GACNNNNGTC 1 cut(s) 293
PshBI ATTAAT 1 cut(s) 477
Psp1406I AACGTT 1 cut(s) 108
Psp6I CCWGG 1 cut(s) 645
PspGI CCWGG 1 cut(s) 645
PspN4I GGNNCC 1 cut(s) 167
PspPI GGNCC 1 cut(s) 165
PsrI GAACNNNNNNTAC 2 cut(s) 183, 215
PstI CTGCAG 2 cut(s) 358, 499
PstNI CAGNNNCTG 1 cut(s) 562
RsaI GTAC 1 cut(s) 229
RsaNI GTAC 1 cut(s) 228
RseI CAYNNNNRTG 1 cut(s) 486
SaqAI TTAA 2 cut(s) 405, 477
SatI GCNGC 3 cut(s) 15, 178, 495
Sau3AI GATC 1 cut(s) 146
Sau96I GGNCC 1 cut(s) 165
ScrFI CCNGG 2 cut(s) 259, 647
SfaNI GCATC 3 cut(s) 349, 354, 631
SfcI CTRYAG 2 cut(s) 354, 495
SinI GGWCC 1 cut(s) 165
SmiMI CAYNNNNRTG 1 cut(s) 486
Sse9I AATT 1 cut(s) 371
SsiI CCGC 4 cut(s) 14, 66, 163, 332
SspI AATATT 1 cut(s) 239
StyD4I CCNGG 2 cut(s) 257, 645
TaaI ACNGT 1 cut(s) 245
TaiI ACGT 4 cut(s) 89, 111, 293, 594
TaqI TCGA 5 cut(s) 149, 397, 551, 594, 615
TasI AATT 1 cut(s) 371
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 1 cut(s) 553
Tru1I TTAA 2 cut(s) 405, 477
Tru9I TTAA 2 cut(s) 405, 477
TscAI CASTG 1 cut(s) 324
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 2 cut(s) 177, 494
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 1 cut(s) 633
TspGWI ACGGA 1 cut(s) 174
TspRI CASTG 1 cut(s) 324
VpaK11BI GGWCC 1 cut(s) 165
VspI ATTAAT 1 cut(s) 477
XmnI GAANNNNTTC 1 cut(s) 461
ZraI GACGTC 1 cut(s) 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.