Rh2BG224200

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0005558
Physical Location & Seq
Reverse (-)
21741867 .. 21743281
1415 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG224200.1

Sequence Viewer

Length: 609 bp
ATGGCGAGTGGTGAGGGGGAAGTGATGAGGAACAAGCAGGTGATATTCAGGGACTATGTGACTGGCAATCCCAAAGAGTCGGACATGTACGTGACAGACAGTAGTACCATTAAGCTCAAGGTTCCACAGGGCTCAGTCGCGGTGCTGGTCAAGAACCTTTACTTGGCGTGTGACCCTTATTTTAGGATCCGAATGGGGCGAATTGAAGGTTCAAGTGTCTTCACTTCCTTCACCCATGGCTCTGGGAAGGTCATTTTCAGATGCACAAAGACAAACCAAAGAGGGGCAAGGACCAAAGCCACGCCCCAAACTCGCAAGCAGCTGCCATCCTTGCCCAGCGTCGGAGGCCAGCAGCGCAGCGCATCACTACACCTCCCAGGGCATCATGACAGGCTTGACAGCAACCCCTCCCATCTACACCTCACATATGCACCCAACGCAGCAGGCACCCTTTCGCCTCACTCCCTTGAACCTCAGAGGCCAACTTCACCATCTCCACCAACAGGTGGATATGATAAGGACACCCACAAGGAAAGAAAGCAAGCTGAAAAGGAAACCATGCCATCCCAAATAAGCAATCCAACCATAACCCATTACCTGAACCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

202

Amino Acids

22.28

Weight (kDa)

9.75

Isoelectric Point (pI)

51.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 12 - 68 8.3e-06 N-terminal domain of oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 28
Acc36I ACCTGC 1 cut(s) 28
AccB1I GGYRCC 1 cut(s) 446
AccB7I CCANNNNNTGG 1 cut(s) 506
AccII CGCG 1 cut(s) 140
AciI CCGC 1 cut(s) 140
AclWI GGATC 2 cut(s) 181, 194
AfaI GTAC 2 cut(s) 89, 106
AfiI CCNNNNNNNGG 5 cut(s) 163, 283, 341, 503, 506
AflIII ACRYGT 1 cut(s) 84
AgsI TTSAA 3 cut(s) 206, 213, 470
AjnI CCWGG 1 cut(s) 376
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
AluBI AGCT 3 cut(s) 115, 322, 545
AluI AGCT 3 cut(s) 115, 322, 545
AlwI GGATC 2 cut(s) 181, 194
AoxI GGCC 2 cut(s) 346, 479
ApeKI GCWGC 5 cut(s) 319, 322, 352, 357, 440
AspLEI GCGC 2 cut(s) 357, 362
AspS9I GGNCC 1 cut(s) 291
AsuHPI GGTGA 4 cut(s) 23, 52, 223, 480
AvaII GGWCC 1 cut(s) 291
BaeI ACNNNNGTAYC 2 cut(s) 88, 121
BamHI GGATCC 1 cut(s) 186
BanI GGYRCC 1 cut(s) 446
BanII GRGCYC 1 cut(s) 134
BbsI GAAGAC 1 cut(s) 211
BbvI GCAGC 5 cut(s) 309, 331, 364, 369, 452
BccI CCATC 4 cut(s) 334, 420, 499, 571
BciT130I CCWGG 1 cut(s) 378
BfmI CTRYAG 1 cut(s) 605
BfuAI ACCTGC 1 cut(s) 28
BisI GCNGC 5 cut(s) 320, 323, 353, 358, 441
BlsI GCNGC 5 cut(s) 321, 324, 354, 359, 442
Bme1390I CCNGG 1 cut(s) 378
Bme18I GGWCC 1 cut(s) 291
BmgT120I GGNCC 1 cut(s) 291
BmiI GGNNCC 3 cut(s) 123, 188, 448
BmrFI CCNGG 1 cut(s) 378
BmsI GCATC 3 cut(s) 251, 371, 391
BpiI GAAGAC 1 cut(s) 211
BpuEI CTTGAG 1 cut(s) 101
BsaAI YACGTR 1 cut(s) 91
BsaJI CCNNGG 3 cut(s) 235, 376, 377
BsaXI ACNNNNNCTCC 2 cut(s) 357, 387
Bsc4I CCNNNNNNNGG 5 cut(s) 163, 283, 341, 503, 506
Bse1I ACTGG 1 cut(s) 67
BseBI CCWGG 1 cut(s) 378
BseDI CCNNGG 3 cut(s) 235, 376, 377
BseGI GGATG 2 cut(s) 326, 563
BseLI CCNNNNNNNGG 5 cut(s) 163, 283, 341, 503, 506
BseMII CTCAG 2 cut(s) 147, 488
BseNI ACTGG 1 cut(s) 67
BseXI GCAGC 5 cut(s) 309, 331, 364, 369, 452
BseYI CCCAGC 1 cut(s) 335
Bsh1236I CGCG 1 cut(s) 140
BshFI GGCC 2 cut(s) 348, 481
BshNI GGYRCC 1 cut(s) 446
BslFI GGGAC 1 cut(s) 65
BslI CCNNNNNNNGG 5 cut(s) 163, 283, 341, 503, 506
BsmFI GGGAC 1 cut(s) 65
BsnI GGCC 2 cut(s) 348, 481
Bsp1286I GDGCHC 1 cut(s) 134
Bsp143I GATC 1 cut(s) 186
Bsp19I CCATGG 1 cut(s) 235
BspACI CCGC 1 cut(s) 140
BspANI GGCC 2 cut(s) 348, 481
BspCNI CTCAG 2 cut(s) 146, 487
BspFNI CGCG 1 cut(s) 140
BspHI TCATGA 1 cut(s) 385
BspLI GGNNCC 3 cut(s) 123, 188, 448
BspMI ACCTGC 1 cut(s) 28
BspPI GGATC 2 cut(s) 181, 194
BspT107I GGYRCC 1 cut(s) 446
BsrI ACTGG 1 cut(s) 67
BssECI CCNNGG 3 cut(s) 235, 376, 377
BssMI GATC 1 cut(s) 186
BssT1I CCWWGG 1 cut(s) 235
Bst2UI CCWGG 1 cut(s) 378
Bst4CI ACNGT 1 cut(s) 101
BstBAI YACGTR 1 cut(s) 91
BstC8I GCNNGC 4 cut(s) 317, 350, 445, 543
BstDEI CTNAG 2 cut(s) 133, 474
BstDSI CCRYGG 1 cut(s) 235
BstF5I GGATG 2 cut(s) 326, 563
BstFNI CGCG 1 cut(s) 140
BstHHI GCGC 2 cut(s) 357, 362
BstKTI GATC 1 cut(s) 189
BstMBI GATC 1 cut(s) 186
BstMWI GCNNNNNNNGC 4 cut(s) 331, 345, 354, 437
BstNI CCWGG 1 cut(s) 378
BstNSI RCATGY 1 cut(s) 88
BstSCI CCNGG 1 cut(s) 376
BstSFI CTRYAG 1 cut(s) 605
BstUI CGCG 1 cut(s) 140
BstV1I GCAGC 5 cut(s) 309, 331, 364, 369, 452
BstV2I GAAGAC 1 cut(s) 211
BstX2I RGATCY 1 cut(s) 186
BstXI CCANNNNNNTGG 1 cut(s) 242
BstYI RGATCY 1 cut(s) 186
BsuRI GGCC 2 cut(s) 348, 481
BtgI CCRYGG 1 cut(s) 235
BtsCI GGATG 2 cut(s) 326, 563
BveI ACCTGC 1 cut(s) 28
Cac8I GCNNGC 4 cut(s) 317, 350, 445, 543
CciI TCATGA 1 cut(s) 385
CfoI GCGC 2 cut(s) 357, 362
Cfr13I GGNCC 1 cut(s) 291
CseI GACGC 1 cut(s) 328
Csp6I GTAC 2 cut(s) 88, 105
CviAII CATG 4 cut(s) 85, 236, 386, 559
CviJI RGCY 9 cut(s) 115, 132, 240, 299, 322, 348, 394, 481, 545
CviKI_1 RGCY 9 cut(s) 115, 132, 240, 299, 322, 348, 394, 481, 545
CviQI GTAC 2 cut(s) 88, 105
DdeI CTNAG 2 cut(s) 133, 474
DpnI GATC 1 cut(s) 188
DpnII GATC 1 cut(s) 186
Eco130I CCWWGG 1 cut(s) 235
Eco24I GRGCYC 1 cut(s) 134
Eco47I GGWCC 1 cut(s) 291
EcoRII CCWGG 1 cut(s) 376
EcoT14I CCWWGG 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 134
ErhI CCWWGG 1 cut(s) 235
FaeI CATG 4 cut(s) 88, 239, 389, 562
FaiI YATR 9 cut(s) 57, 86, 237, 387, 427, 429, 513, 560, 587
FaqI GGGAC 1 cut(s) 65
FatI CATG 4 cut(s) 84, 235, 385, 558
FauNDI CATATG 1 cut(s) 427
Fnu4HI GCNGC 5 cut(s) 320, 323, 353, 358, 441
FokI GGATG 2 cut(s) 313, 550
FriOI GRGCYC 1 cut(s) 134
Fsp4HI GCNGC 5 cut(s) 320, 323, 353, 358, 441
GlaI GCGC 2 cut(s) 356, 361
GluI GCNGC 5 cut(s) 320, 323, 353, 358, 441
GsaI CCCAGC 1 cut(s) 339
HaeIII GGCC 2 cut(s) 348, 481
HgaI GACGC 1 cut(s) 328
HhaI GCGC 2 cut(s) 357, 362
Hin1II CATG 4 cut(s) 88, 239, 389, 562
Hin6I GCGC 2 cut(s) 355, 360
HinP1I GCGC 2 cut(s) 355, 360
HinfI GANTC 1 cut(s) 77
HphI GGTGA 4 cut(s) 23, 52, 223, 480
Hpy188I TCNGA 5 cut(s) 82, 191, 260, 344, 477
Hpy188III TCNNGA 2 cut(s) 151, 386
Hpy99I CGWCG 1 cut(s) 344
HpyAV CCTTC 3 cut(s) 200, 238, 241
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 2 cut(s) 264, 431
HpyF10VI GCNNNNNNNGC 4 cut(s) 331, 345, 354, 437
HpyF3I CTNAG 2 cut(s) 133, 474
HpySE526I ACGT 1 cut(s) 90
Hsp92II CATG 4 cut(s) 88, 239, 389, 562
HspAI GCGC 2 cut(s) 355, 360
Kzo9I GATC 1 cut(s) 186
Lsp1109I GCAGC 5 cut(s) 309, 331, 364, 369, 452
LweI GCATC 3 cut(s) 251, 371, 391
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 3 cut(s) 58, 91, 170
MalI GATC 1 cut(s) 188
MboI GATC 1 cut(s) 186
MboII GAAGA 1 cut(s) 211
MflI RGATCY 1 cut(s) 186
MhlI GDGCHC 1 cut(s) 134
MluCI AATT 1 cut(s) 201
MlyI GAGTC 1 cut(s) 86
MmeI TCCRAC 3 cut(s) 60, 322, 605
MseI TTAA 1 cut(s) 111
MslI CAYNNNNRTG 1 cut(s) 89
MspA1I CMGCKG 1 cut(s) 322
MspR9I CCNGG 1 cut(s) 378
MteI GCGCNGCGC 1 cut(s) 358
MvaI CCWGG 1 cut(s) 378
MvnI CGCG 1 cut(s) 140
MwoI GCNNNNNNNGC 4 cut(s) 331, 345, 354, 437
NcoI CCATGG 1 cut(s) 235
NdeI CATATG 1 cut(s) 427
NdeII GATC 1 cut(s) 186
NlaIII CATG 4 cut(s) 88, 239, 389, 562
NlaIV GGNNCC 3 cut(s) 123, 188, 448
NmuCI GTSAC 3 cut(s) 58, 91, 170
NspI RCATGY 1 cut(s) 88
PagI TCATGA 1 cut(s) 385
PaqCI CACCTGC 1 cut(s) 28
PasI CCCWGGG 1 cut(s) 377
PciI ACATGT 1 cut(s) 84
PflMI CCANNNNNTGG 1 cut(s) 506
PkrI GCNGC 5 cut(s) 321, 324, 354, 359, 442
PleI GAGTC 1 cut(s) 85
PpsI GAGTC 1 cut(s) 85
Ppu21I YACGTR 1 cut(s) 91
PscI ACATGT 1 cut(s) 84
Psp6I CCWGG 1 cut(s) 376
PspFI CCCAGC 1 cut(s) 335
PspGI CCWGG 1 cut(s) 376
PspN4I GGNNCC 3 cut(s) 123, 188, 448
PspPI GGNCC 1 cut(s) 291
PsuI RGATCY 1 cut(s) 186
PvuII CAGCTG 1 cut(s) 322
RsaI GTAC 2 cut(s) 89, 106
RsaNI GTAC 2 cut(s) 88, 105
RseI CAYNNNNRTG 1 cut(s) 89
SaqAI TTAA 1 cut(s) 111
SatI GCNGC 5 cut(s) 320, 323, 353, 358, 441
Sau3AI GATC 1 cut(s) 186
Sau96I GGNCC 1 cut(s) 291
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 1 cut(s) 378
SduI GDGCHC 1 cut(s) 134
SfaNI GCATC 3 cut(s) 251, 371, 391
SfcI CTRYAG 1 cut(s) 605
SinI GGWCC 1 cut(s) 291
SmiMI CAYNNNNRTG 1 cut(s) 89
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
Sse9I AATT 1 cut(s) 201
SsiI CCGC 1 cut(s) 140
StyD4I CCNGG 1 cut(s) 376
StyI CCWWGG 1 cut(s) 235
TaaI ACNGT 1 cut(s) 101
TaiI ACGT 1 cut(s) 93
TasI AATT 1 cut(s) 201
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TseFI GTSAC 3 cut(s) 58, 91, 170
TseI GCWGC 5 cut(s) 319, 322, 352, 357, 440
Tsp45I GTSAC 3 cut(s) 58, 91, 170
Van91I CCANNNNNTGG 1 cut(s) 506
VpaK11BI GGWCC 1 cut(s) 291
XceI RCATGY 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.