Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0005558

Physical Location & Seq

Genomic Coordinates Forward (+)

35745653 .. 35752122

Gene Structure 6470 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0311000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2547 bp

ATGAGTGCCCTCAAGATCACCAAAAAGCACCACAAACACTTGAACAATCCTTTCCCTTCCGCACCTCTCTCTCTCCCGCTCATCCAAGGAAACCTTGTCTTTAATTCTGAAACGGTGCCTTCACCCCAACGTTTTGATATTGGAAAGGATTTTGAGCTGTCTTGGAACACCAGCAATGGCGGGTCTTTGTCAATTTCTCACCGATCGCAGCCCAGGAGAGCCATATGGTCCACCGTTCCTGGTCAAGCCTTTGTCTCTGCAGCAGTGGCTCAAACACGAGTGGAAGAAAGCAGGGGTTCTTTTGTGGTCGAAGACATTTGTGTTGATCTGGTTTGCCATCACCAAACCATTCAAGACATAAGGGTGATCAGTCAGTCTGATCATCATGATTCTCCATCTGGGTTTTCAGGGCTGGACCTGGAATCAGATTCTCCAGGTACCCAATTCCCTATTGTCCTTGTAACTGGCTGGGTTTTCAATACGGAGAGGAGGAAGAAGCGTTTTCTGAAATTTGAGGGGAAGGGATCTTCTGCTTGTGCAAAGTATTGGGTTTTGTTTGAACAGAAATGCAGTCATCAAATTGGGTTCCAAGTGAAGCTCGGACAACCCAACTTTGAATTTCCCACCAAAGCAGCTACTTACAAGGGTTTTAGAACTAGGCTGCTGGGTCGGTTTCAGAAACCCAGGCTGCGGTGGTCTTGGTCCTTTACGAGGCCCAGAGGGTTTGTCTTGCTTCCCTCTGAGGCTCAACAATTCAAAGAATTCAACAGGGTGTGCCTGACATATTCAAGTGAAGAAAAGGAGAGGTTTTATGGATTTGGGGAGCAATTCTCTTTTATGGATTTTAAGGGCAAAAGGGTGCCCATTTTTGTTCAGGAACAGGGTATTGGGAGAGGAGATCAGCCTATTACCTTTGCAGCTAACTTAGTCAGTTACAGGGCTGGCGGCGATTGGAGTACAACCTATGCTCCTTCCCCATTCTATATGACTTCAAAGATGAAATCTCTTTACCTTGAAGGATATGATTATTGCGTATTTGATCTAACACAACATGACAAAGTTCAGATAAAGATACATGGGAATTCAGTAGAGGGACGGATATTGCATGGAAACTCACCGACTGAGCTCATTGAATGTTTCACTGAAACCATTGGGAGACCTCCTAAACTTCCGGACTGGATTATTTCTGGAGCTGTTGTTGGCATGCAGGGTGGCACAAAATCTGTACGTCGTATTTGGAATGAGCTTAAGAGTTACAATGCTCCAGTTTCAGCATTCTGGTTGCAGGATTGGGTGGGCCACAGAGAGACATTGGTTGGATCACAACTCTGGTGGAATTGGGAAGTAGATTCAACAAGGTATATGGGATGGCAACAACTAATCAAAGATCTTAGTGCTCAGCATATTAAAGTGATGACATACTGCAACCCATGTCTAGCTCCGTGTCATGAAAAGTCAAACATAAGGAGAAACCTCTTTGAGGAAGCAAAGAAGTTGGACATATTAGTGAAAGACAAGCAAGGAGAACCTTATATGGTTCCAAATACTGCTTTTGATGTAGGAATGTTGGACTTGACACATCCAGATACTGGAAATTGGTTCAAGCAGATTCTTCAGGAAATGGTTGATGATGGAGTCAGAGGGTGGATGGCTGATTTTGGTGAAGGCCTGCCTGTGGATGCCACCCTCTATTCAGGCGAAGATCCTATTTCAGCCCACAACAAGTACCCAGAGCTATGGGCCCAAATAAATAGAGAGTTTGTGGAAGAATGGAAAGCTAACCGTGTGGGAAAGGAGAAAGAAGATCCACAAGAGGCCTTGGTCTTTTTCATGAGGGCTGGTTTCAGAGATAGCCCCAAATGGGGAATGCTATTTTGGGAAGGAGATCAGATGGTAAGTTGGCAGATTCATGACGGAATAAAGAGTGCTGTTGTTGGTCTACTCAGCAGTGGAATTTCAGGGTATGCTTTCAATCACAGTGATATTGGAGGGTACTGTGCTGTAAATTTACCTTTCATCAAGTATCGAAGAAGTGAAGAATTGCTTTTGCGATGGATGGAGCTAAATGCTTTCACCACTGTTTTCCGGACACATGAAGGAAACAAACCATCTTGCAACAGTCAATTCTACTCAAATGAGAGAACTCTAGCTCATTTTGCACGATTTGCTAAAGTCTATATAGCTTGGAAGTTCTATAGGATCCAGCTCGTCGAGGAAGCGACTCATAGAGGCCTGCCTGTATGCCGCCACTTATTTCTGCATTACCCTAATGATGAACATGTTCATAATTTGAGTTACCAGCAGTTCTTGATTGGTACTGAGATTCTGGTGGTGCCTGTCCTCGACAAAGGTATGAACAATGTGAAGGCTTATTTTCCAACTGGTAATGACTGTTGGCAACATGTATGGACTGGAAAGCAGTTTACTGAAGAGGGTTTTGAAACCATAGTAGAAGCTCAAATTGGTTACCCTGCTGTATTTGTTAAGACTGGTTCCGTTGTTGGAGAGACGTTTTTGAAAAACCTCAGAGATTTGAAAATTCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

848

Amino Acids

97.13

Weight (kDa)

8.06

Isoelectric Point (pI)

45.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_31_2nd	PF01055	405 - 714	1.1e-36	Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd	PF21365	744 - 834	3.4e-16	Glycosyl hydrolase family 31 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005558)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr1g0329171 RchiOBHm_Chr3g0465871 RchiOBHm_Chr3g0465881 RchiOBHm_Chr5g0077491
rosa_laevigata	RLG00000003324 RLG00000018512 RLG00000022062
rosa_multiflora	Rmu_co8434517.1_g000001 Rmu_sc0000704.1_g000010 Rmu_sc0001524.1_g000020 Rmu_sc0003323.1_g000019 Rmu_sc0005816.1_g000009 Rmu_sc0007705.1_g000011 Rmu_sc0009386.1_g000005 Rmu_sc0011593.1_g000008
rosa_roxburghii	Rroxscaffold_5G00362020
rosa_rugosa	Rorug02G0311000
rosa_samantha	Rh1BG155000 Rh1CG078400 Rh1DG084800 Rh1DG455000 Rh2BG224200 Rh3BG145100 Rh3DG146200 Rh5AG378500 Rh5AG506900 Rh5CG262800 Rh5CG400500
rosa_wichuraiana	Rw3G011730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	437
AccB1I	GGYRCC	4 cut(s)	115, 437, 859, 2332
AccB7I	CCANNNNNTGG	1 cut(s)	1589
AccBSI	CCGCTC	1 cut(s)	79
AccI	GTMKAC	1 cut(s)	1939
AccIII	TCCGGA	2 cut(s)	1171, 2085
AciI	CCGC	6 cut(s)	60, 77, 180, 691, 945, 2245
AclI	AACGTT	1 cut(s)	130
AclWI	GGATC	6 cut(s)	532, 1327, 1697, 1799, 2194, 2207
AcsI	RAATTY	7 cut(s)	509, 617, 761, 1081, 1953, 2005, 2538
AcuI	CTGAAG	2 cut(s)	1598, 2448
AfaI	GTAC	6 cut(s)	439, 958, 1227, 1727, 1994, 2317
AfiI	CCNNNNNNNGG	7 cut(s)	690, 711, 1480, 1589, 1675, 1861, 1862
AflII	CTTAAG	1 cut(s)	1247
AflIII	ACRYGT	2 cut(s)	2278, 2401
AjnI	CCWGG	5 cut(s)	212, 238, 417, 433, 683
AjuI	GAANNNNNNNTTGG	6 cut(s)	870, 902, 1858, 1890, 2445, 2477
Alw21I	GWGCWC	2 cut(s)	1128, 1399
Alw26I	GTCTC	4 cut(s)	259, 1150, 1301, 2501
AlwI	GGATC	6 cut(s)	532, 1327, 1697, 1799, 2194, 2207
AlwNI	CAGNNNCTG	1 cut(s)	1589
Aor13HI	TCCGGA	2 cut(s)	1171, 2085
AoxI	GGCC	6 cut(s)	713, 1297, 1666, 1740, 1815, 2230
ApaI	GGGCCC	1 cut(s)	1744
ApeKI	GCWGC	6 cut(s)	208, 260, 632, 661, 688, 917
ApoI	RAATTY	7 cut(s)	509, 617, 761, 1081, 1953, 2005, 2538
Asp700I	GAANNNNTTC	1 cut(s)	2280
Asp718I	GGTACC	1 cut(s)	437
AspS9I	GGNCC	7 cut(s)	228, 415, 702, 714, 1297, 1740, 1741
AsuHPI	GGTGA	8 cut(s)	10, 114, 191, 332, 376, 1107, 1673, 2065
AvaII	GGWCC	3 cut(s)	228, 415, 702
BaeGI	GKGCMC	3 cut(s)	10, 864, 1744
BamHI	GGATCC	1 cut(s)	2199
BanI	GGYRCC	4 cut(s)	115, 437, 859, 2332
BanII	GRGCYC	2 cut(s)	1128, 1744
BauI	CACGAG	1 cut(s)	276
BbsI	GAAGAC	1 cut(s)	318
Bbv12I	GWGCWC	2 cut(s)	1128, 1399
BbvI	GCAGC	6 cut(s)	220, 272, 644, 648, 675, 929
BccI	CCATC	9 cut(s)	345, 403, 1362, 1625, 1642, 1885, 2046, 2050, 2116
BciT130I	CCWGG	5 cut(s)	214, 240, 419, 435, 685
BclI	TGATCA	2 cut(s)	366, 379
BcoDI	GTCTC	4 cut(s)	259, 1150, 1301, 2501
BfaI	CTAG	3 cut(s)	657, 1436, 2147
BfmI	CTRYAG	2 cut(s)	258, 2194
BfrI	CTTAAG	1 cut(s)	1247
BglII	AGATCT	1 cut(s)	1387
BisI	GCNGC	8 cut(s)	209, 261, 633, 662, 689, 918, 946, 2245
BlpI	GCTNAGC	1 cut(s)	1398
BlsI	GCNGC	8 cut(s)	210, 262, 634, 663, 690, 919, 947, 2246
Bme1390I	CCNGG	5 cut(s)	214, 240, 419, 435, 685
Bme18I	GGWCC	3 cut(s)	228, 415, 702
BmgT120I	GGNCC	7 cut(s)	228, 415, 702, 714, 1297, 1740, 1741
BmiI	GGNNCC	9 cut(s)	117, 439, 587, 861, 1539, 1742, 2201, 2334, 2494
BmrFI	CCNGG	5 cut(s)	214, 240, 419, 435, 685
BmsI	GCATC	1 cut(s)	1669
BpiI	GAAGAC	1 cut(s)	318
BplI	GAGNNNNNCTC	2 cut(s)	815, 847
BpmI	CTGGAG	3 cut(s)	417, 1209, 1248
Bpu1102I	GCTNAGC	1 cut(s)	1398
BsaI	GGTCTC	1 cut(s)	1150
BsaJI	CCNNGG	4 cut(s)	85, 212, 683, 1818
BsaWI	WCCGGW	2 cut(s)	1171, 2085
BsaXI	ACNNNNNCTCC	4 cut(s)	952, 982, 1182, 1212
Bsc4I	CCNNNNNNNGG	7 cut(s)	690, 711, 1480, 1589, 1675, 1861, 1862
Bse1I	ACTGG	7 cut(s)	469, 1181, 1265, 1594, 2386, 2416, 2494
Bse3DI	GCAATG	1 cut(s)	181
BseAI	TCCGGA	2 cut(s)	1171, 2085
BseBI	CCWGG	5 cut(s)	214, 240, 419, 435, 685
BseDI	CCNNGG	4 cut(s)	85, 212, 683, 1818
BseGI	GGATG	6 cut(s)	81, 1373, 1579, 1653, 1684, 2061
BseLI	CCNNNNNNNGG	7 cut(s)	690, 711, 1480, 1589, 1675, 1861, 1862
BseMI	GCAATG	1 cut(s)	181
BseMII	CTCAG	6 cut(s)	732, 1113, 1412, 1957, 2310, 2539
BseNI	ACTGG	7 cut(s)	469, 1181, 1265, 1594, 2386, 2416, 2494
BseRI	GAGGAG	2 cut(s)	502, 909
BseSI	GKGCMC	3 cut(s)	10, 864, 1744
BseXI	GCAGC	6 cut(s)	220, 272, 644, 648, 675, 929
BseYI	CCCAGC	2 cut(s)	468, 664
Bsh1285I	CGRYCG	1 cut(s)	206
BshFI	GGCC	6 cut(s)	715, 1299, 1668, 1742, 1817, 2232
BshNI	GGYRCC	4 cut(s)	115, 437, 859, 2332
BsiEI	CGRYCG	1 cut(s)	206
BsiHKAI	GWGCWC	2 cut(s)	1128, 1399
BsiSI	CCGG	2 cut(s)	1172, 2086
BslFI	GGGAC	1 cut(s)	1107
BslI	CCNNNNNNNGG	7 cut(s)	690, 711, 1480, 1589, 1675, 1861, 1862
BsmAI	GTCTC	4 cut(s)	259, 1150, 1301, 2501
BsmBI	CGTCTC	1 cut(s)	2501
BsmFI	GGGAC	1 cut(s)	1107
BsmI	GAATGC	2 cut(s)	1274, 1872
BsnI	GGCC	6 cut(s)	715, 1299, 1668, 1742, 1817, 2232
Bso31I	GGTCTC	1 cut(s)	1150
Bsp120I	GGGCCC	1 cut(s)	1740
Bsp1286I	GDGCHC	5 cut(s)	10, 864, 1128, 1399, 1744
Bsp13I	TCCGGA	2 cut(s)	1171, 2085
Bsp1720I	GCTNAGC	1 cut(s)	1398
BspACI	CCGC	6 cut(s)	60, 77, 180, 691, 945, 2245
BspANI	GGCC	6 cut(s)	715, 1299, 1668, 1742, 1817, 2232
BspCNI	CTCAG	6 cut(s)	733, 1114, 1411, 1956, 2311, 2538
BspEI	TCCGGA	2 cut(s)	1171, 2085
BspHI	TCATGA	4 cut(s)	385, 1447, 1830, 1909
BspLI	GGNNCC	9 cut(s)	117, 439, 587, 861, 1539, 1742, 2201, 2334, 2494
BspMAI	CTGCAG	1 cut(s)	262
BspPI	GGATC	6 cut(s)	532, 1327, 1697, 1799, 2194, 2207
BspT107I	GGYRCC	4 cut(s)	115, 437, 859, 2332
BspTI	CTTAAG	1 cut(s)	1247
BspTNI	GGTCTC	1 cut(s)	1150
BsrBI	CCGCTC	1 cut(s)	79
BsrDI	GCAATG	1 cut(s)	181
BsrI	ACTGG	7 cut(s)	469, 1181, 1265, 1594, 2386, 2416, 2494
BssECI	CCNNGG	4 cut(s)	85, 212, 683, 1818
BssSI	CACGAG	1 cut(s)	276
BssT1I	CCWWGG	2 cut(s)	85, 1818
Bst2BI	CACGAG	1 cut(s)	276
Bst2UI	CCWGG	5 cut(s)	214, 240, 419, 435, 685
Bst4CI	ACNGT	8 cut(s)	115, 235, 1784, 1979, 1997, 2080, 2120, 2393
Bst6I	CTCTTC	1 cut(s)	2424
BstAFI	CTTAAG	1 cut(s)	1247
BstAPI	GCANNNNNTGC	1 cut(s)	2165
BstC8I	GCNNGC	4 cut(s)	943, 1205, 1670, 2234
BstDEI	CTNAG	8 cut(s)	741, 925, 1122, 1391, 1398, 1943, 2319, 2525
BstEII	GGTNACC	1 cut(s)	2465
BstENI	CCTNNNNNAGG	2 cut(s)	709, 1478
BstF5I	GGATG	6 cut(s)	81, 1373, 1579, 1653, 1684, 2061
BstMAI	GTCTC	4 cut(s)	259, 1150, 1301, 2501
BstMCI	CGRYCG	1 cut(s)	206
BstMWI	GCNNNNNNNGC	3 cut(s)	266, 2156, 2165
BstNI	CCWGG	5 cut(s)	214, 240, 419, 435, 685
BstNSI	RCATGY	3 cut(s)	1207, 2282, 2405
BstPI	GGTNACC	1 cut(s)	2465
BstSCI	CCNGG	5 cut(s)	212, 238, 417, 433, 683
BstSFI	CTRYAG	2 cut(s)	258, 2194
BstSLI	GKGCMC	3 cut(s)	10, 864, 1744
BstV1I	GCAGC	6 cut(s)	220, 272, 644, 648, 675, 929
BstV2I	GAAGAC	1 cut(s)	318
BstX2I	RGATCY	5 cut(s)	524, 1387, 1702, 1804, 2199
BstXI	CCANNNNNNTGG	1 cut(s)	1737
BstYI	RGATCY	5 cut(s)	524, 1387, 1702, 1804, 2199
BsuRI	GGCC	6 cut(s)	715, 1299, 1668, 1742, 1817, 2232
BtgZI	GCGATG	1 cut(s)	2065
BtsCI	GGATG	6 cut(s)	81, 1373, 1579, 1653, 1684, 2061
BtsI	GCAGTG	2 cut(s)	270, 1954
BtsIMutI	CAGTG	5 cut(s)	270, 1140, 1954, 1984, 2076
Cac8I	GCNNGC	4 cut(s)	943, 1205, 1670, 2234
CaiI	CAGNNNCTG	1 cut(s)	1589
CciI	TCATGA	4 cut(s)	385, 1447, 1830, 1909
Cfr13I	GGNCC	7 cut(s)	228, 415, 702, 714, 1297, 1740, 1741
Csp6I	GTAC	6 cut(s)	438, 957, 1226, 1726, 1993, 2316
CspCI	CAANNNNNGTGG	2 cut(s)	1313, 1348
CviQI	GTAC	6 cut(s)	438, 957, 1226, 1726, 1993, 2316
DdeI	CTNAG	8 cut(s)	741, 925, 1122, 1391, 1398, 1943, 2319, 2525
Eam1104I	CTCTTC	1 cut(s)	2424
EarI	CTCTTC	1 cut(s)	2424
Ecl136II	GAGCTC	1 cut(s)	1126
Eco130I	CCWWGG	2 cut(s)	85, 1818
Eco147I	AGGCCT	3 cut(s)	1668, 1817, 2232
Eco24I	GRGCYC	2 cut(s)	1128, 1744
Eco31I	GGTCTC	1 cut(s)	1150
Eco47I	GGWCC	3 cut(s)	228, 415, 702
Eco53kI	GAGCTC	1 cut(s)	1126
Eco57I	CTGAAG	2 cut(s)	1598, 2448
Eco91I	GGTNACC	1 cut(s)	2465
EcoICRI	GAGCTC	1 cut(s)	1126
EcoNI	CCTNNNNNAGG	2 cut(s)	709, 1478
EcoO65I	GGTNACC	1 cut(s)	2465
EcoRI	GAATTC	2 cut(s)	761, 1081
EcoRII	CCWGG	5 cut(s)	212, 238, 417, 433, 683
EcoT14I	CCWWGG	2 cut(s)	85, 1818
EcoT38I	GRGCYC	2 cut(s)	1128, 1744
ErhI	CCWWGG	2 cut(s)	85, 1818
Esp3I	CGTCTC	1 cut(s)	2501
FalI	AAGNNNNNCTT	4 cut(s)	78, 110, 2028, 2060
FaqI	GGGAC	1 cut(s)	1107
FauI	CCCGC	2 cut(s)	84, 173
FauNDI	CATATG	1 cut(s)	224
FbaI	TGATCA	2 cut(s)	366, 379
FblI	GTMKAC	1 cut(s)	1939
Fnu4HI	GCNGC	8 cut(s)	209, 261, 633, 662, 689, 918, 946, 2245
FokI	GGATG	6 cut(s)	68, 1380, 1566, 1660, 1691, 2068
FriOI	GRGCYC	2 cut(s)	1128, 1744
Fsp4HI	GCNGC	8 cut(s)	209, 261, 633, 662, 689, 918, 946, 2245
FspBI	CTAG	3 cut(s)	657, 1436, 2147
GluI	GCNGC	8 cut(s)	209, 261, 633, 662, 689, 918, 946, 2245
GsaI	CCCAGC	2 cut(s)	472, 668
GsuI	CTGGAG	3 cut(s)	417, 1209, 1248
HaeIII	GGCC	6 cut(s)	715, 1299, 1668, 1742, 1817, 2232
HapII	CCGG	2 cut(s)	1172, 2086
HinfI	GANTC	9 cut(s)	389, 422, 428, 1349, 1609, 1635, 1906, 2221, 2323
HpaII	CCGG	2 cut(s)	1172, 2086
HphI	GGTGA	8 cut(s)	10, 114, 191, 332, 376, 1107, 1673, 2065
Hpy166II	GTNNAC	3 cut(s)	231, 1940, 2424
Hpy8I	GTNNAC	3 cut(s)	231, 1940, 2424
Hpy99I	CGWCG	2 cut(s)	1233, 2213
HpyAV	CCTTC	9 cut(s)	66, 129, 514, 981, 1010, 1658, 1874, 2090, 2359
HpyCH4III	ACNGT	8 cut(s)	115, 235, 1784, 1979, 1997, 2080, 2120, 2393
HpyCH4IV	ACGT	3 cut(s)	130, 1228, 2510
HpyF10VI	GCNNNNNNNGC	3 cut(s)	266, 2156, 2165
HpyF3I	CTNAG	8 cut(s)	741, 925, 1122, 1391, 1398, 1943, 2319, 2525
HpySE526I	ACGT	3 cut(s)	130, 1228, 2510
Kpn2I	TCCGGA	2 cut(s)	1171, 2085
KpnI	GGTACC	1 cut(s)	441
Ksp22I	TGATCA	2 cut(s)	366, 379
LmnI	GCTCC	6 cut(s)	823, 973, 1190, 1267, 1444, 2059
Lsp1109I	GCAGC	6 cut(s)	220, 272, 644, 648, 675, 929
LweI	GCATC	1 cut(s)	1669
MaeI	CTAG	3 cut(s)	657, 1436, 2147
MaeII	ACGT	3 cut(s)	130, 1228, 2510
MaeIII	GTNAC	5 cut(s)	460, 932, 1253, 2294, 2465
MbiI	CCGCTC	1 cut(s)	79
MflI	RGATCY	5 cut(s)	524, 1387, 1702, 1804, 2199
MhlI	GDGCHC	5 cut(s)	10, 864, 1128, 1399, 1744
MlyI	GAGTC	2 cut(s)	1644, 2215
MmeI	TCCRAC	5 cut(s)	1297, 1476, 1548, 2402, 2482
MroI	TCCGGA	2 cut(s)	1171, 2085
MroXI	GAANNNNTTC	1 cut(s)	2280
MseI	TTAA	6 cut(s)	102, 846, 1248, 1407, 2484, 2545
MslI	CAYNNNNRTG	2 cut(s)	362, 1505
MspCI	CTTAAG	1 cut(s)	1247
MspI	CCGG	2 cut(s)	1172, 2086
MspR9I	CCNGG	5 cut(s)	214, 240, 419, 435, 685
Mva1269I	GAATGC	2 cut(s)	1274, 1872
MvaI	CCWGG	5 cut(s)	214, 240, 419, 435, 685
MwoI	GCNNNNNNNGC	3 cut(s)	266, 2156, 2165
NdeI	CATATG	1 cut(s)	224
NlaIV	GGNNCC	9 cut(s)	117, 439, 587, 861, 1539, 1742, 2201, 2334, 2494
NspI	RCATGY	3 cut(s)	1207, 2282, 2405
PaeI	GCATGC	1 cut(s)	1207
PagI	TCATGA	4 cut(s)	385, 1447, 1830, 1909
PceI	AGGCCT	3 cut(s)	1668, 1817, 2232
PciI	ACATGT	2 cut(s)	2278, 2401
PctI	GAATGC	2 cut(s)	1274, 1872
PdmI	GAANNNNTTC	1 cut(s)	2280
PfeI	GAWTC	7 cut(s)	389, 422, 428, 1349, 1609, 1906, 2323
PflMI	CCANNNNNTGG	1 cut(s)	1589
PkrI	GCNGC	8 cut(s)	210, 262, 634, 663, 690, 919, 947, 2246
Ple19I	CGATCG	1 cut(s)	206
PleI	GAGTC	2 cut(s)	1643, 2215
PpsI	GAGTC	2 cut(s)	1643, 2215
PscI	ACATGT	2 cut(s)	2278, 2401
Psp124BI	GAGCTC	1 cut(s)	1128
Psp1406I	AACGTT	1 cut(s)	130
Psp6I	CCWGG	5 cut(s)	212, 238, 417, 433, 683
PspEI	GGTNACC	1 cut(s)	2465
PspFI	CCCAGC	2 cut(s)	468, 664
PspGI	CCWGG	5 cut(s)	212, 238, 417, 433, 683
PspN4I	GGNNCC	9 cut(s)	117, 439, 587, 861, 1539, 1742, 2201, 2334, 2494
PspOMI	GGGCCC	1 cut(s)	1740
PspPI	GGNCC	7 cut(s)	228, 415, 702, 714, 1297, 1740, 1741
PstI	CTGCAG	1 cut(s)	262
PstNI	CAGNNNCTG	1 cut(s)	1589
PsuI	RGATCY	5 cut(s)	524, 1387, 1702, 1804, 2199
PvuI	CGATCG	1 cut(s)	206
RsaI	GTAC	6 cut(s)	439, 958, 1227, 1727, 1994, 2317
RsaNI	GTAC	6 cut(s)	438, 957, 1226, 1726, 1993, 2316
RseI	CAYNNNNRTG	2 cut(s)	362, 1505
SacI	GAGCTC	1 cut(s)	1128
SaqAI	TTAA	6 cut(s)	102, 846, 1248, 1407, 2484, 2545
SatI	GCNGC	8 cut(s)	209, 261, 633, 662, 689, 918, 946, 2245
Sau96I	GGNCC	7 cut(s)	228, 415, 702, 714, 1297, 1740, 1741
SchI	GAGTC	2 cut(s)	1644, 2215
ScrFI	CCNGG	5 cut(s)	214, 240, 419, 435, 685
SduI	GDGCHC	5 cut(s)	10, 864, 1128, 1399, 1744
SfaNI	GCATC	1 cut(s)	1669
SfcI	CTRYAG	2 cut(s)	258, 2194
SinI	GGWCC	3 cut(s)	228, 415, 702
SmiMI	CAYNNNNRTG	2 cut(s)	362, 1505
SmlI	CTYRAG	2 cut(s)	11, 1247
SmoI	CTYRAG	2 cut(s)	11, 1247
SphI	GCATGC	1 cut(s)	1207
SseBI	AGGCCT	3 cut(s)	1668, 1817, 2232
SsiI	CCGC	6 cut(s)	60, 77, 180, 691, 945, 2245
SspMI	CTAG	3 cut(s)	657, 1436, 2147
SstI	GAGCTC	1 cut(s)	1128
StuI	AGGCCT	3 cut(s)	1668, 1817, 2232
StyD4I	CCNGG	5 cut(s)	212, 238, 417, 433, 683
StyI	CCWWGG	2 cut(s)	85, 1818
TaaI	ACNGT	8 cut(s)	115, 235, 1784, 1979, 1997, 2080, 2120, 2393
TaiI	ACGT	3 cut(s)	133, 1231, 2513
TaqI	TCGA	4 cut(s)	309, 2026, 2211, 2343
TatI	WGTACW	1 cut(s)	956
TauI	GCSGC	2 cut(s)	948, 2247
TfiI	GAWTC	7 cut(s)	389, 422, 428, 1349, 1609, 1906, 2323
Tru1I	TTAA	6 cut(s)	102, 846, 1248, 1407, 2484, 2545
Tru9I	TTAA	6 cut(s)	102, 846, 1248, 1407, 2484, 2545
TscAI	CASTG	5 cut(s)	270, 1147, 1954, 1984, 2083
TseI	GCWGC	6 cut(s)	208, 260, 632, 661, 688, 917
TspDTI	ATGAA	9 cut(s)	1013, 1464, 1819, 1898, 2005, 2109, 2273, 2289, 2369
TspGWI	ACGGA	5 cut(s)	497, 1111, 1431, 1929, 2485
TspRI	CASTG	5 cut(s)	270, 1147, 1954, 1984, 2083
Van91I	CCANNNNNTGG	1 cut(s)	1589
Vha464I	CTTAAG	1 cut(s)	1247
VpaK11BI	GGWCC	3 cut(s)	228, 415, 702
XagI	CCTNNNNNAGG	2 cut(s)	709, 1478
XapI	RAATTY	7 cut(s)	509, 617, 761, 1081, 1953, 2005, 2538
XceI	RCATGY	3 cut(s)	1207, 2282, 2405
XmiI	GTMKAC	1 cut(s)	1939
XmnI	GAANNNNTTC	1 cut(s)	2280
XspI	CTAG	3 cut(s)	657, 1436, 2147

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.