Rroxscaffold_1G00066450

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003327
Physical Location & Seq
Forward (+)
87993029 .. 87993633
605 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00066450.1

Sequence Viewer

Length: 558 bp
ATGCACGAAGGTTCGTGCTGCGCATTTGCGGCCGTAGCAGGAGTCGAAAGGATTGCACAAATCAGGAACAGAGAAAACCCAGCTCGACTATCCGTGCAGCAGCTCGTTGATTGTGATGAGAAGTATAACAGAGGCTGCAGAGGCGGTCTCGCAAGATATGCCTACGACTACATAGTAAGGAACGGAGGCATCACCAGTGCCGAAAATTACCCATATACTGATTCGGAAGGACAGTGCTACCTCGCTAAGGAAAAACAATTTGATGCAAGTATCACTGGGTTTACTCTCGTGCCGCCAAATGACGAGTTTGAGCTGATGAAGGCGCGCTACACTGGTGGAGCATTCAGTGGAGAGTGTGGAACGGAGTTAGGGCACGCTGTGACCATTGTTGGAATGGGGAAGACTGATGACGGCATTAAGTACTGGAAATTGATGAATTCGTGGGGTGAGATGTGGGGCGAAGGAGGGTATATTAGAATTCTCAGAGGAACTGGCAAGCCAGAAGGTCATTGTGGCATAAATATTGAACCTTCTTATCCCGATATTGGTGACCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko04621 ko04924 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map04621 map04924 map05205 map05323 map05418
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

185

Amino Acids

20.31

Weight (kDa)

5.13

Isoelectric Point (pI)

27.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C1 PF00112 4 - 112 2.7e-25 Papain family cysteine protease
Peptidase_C1 PF00112 109 - 180 7.7e-18 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 22
AccII CGCG 1 cut(s) 325
AciI CCGC 3 cut(s) 29, 144, 293
AcoI YGGCCR 1 cut(s) 30
AcsI RAATTY 2 cut(s) 436, 477
AdeI CACNNNGTG 1 cut(s) 379
AfaI GTAC 1 cut(s) 422
AfiI CCNNNNNNNGG 2 cut(s) 247, 545
AgsI TTSAA 1 cut(s) 527
AluBI AGCT 3 cut(s) 83, 103, 313
AluI AGCT 3 cut(s) 83, 103, 313
Alw26I GTCTC 1 cut(s) 152
AlwNI CAGNNNCTG 1 cut(s) 135
AoxI GGCC 1 cut(s) 30
ApeKI GCWGC 4 cut(s) 18, 97, 100, 135
ApoI RAATTY 2 cut(s) 436, 477
AspLEI GCGC 3 cut(s) 23, 325, 327
AsuHPI GGTGA 2 cut(s) 184, 458
BaeGI GKGCMC 1 cut(s) 375
BarI GAAGNNNNNNTAC 2 cut(s) 311, 343
BauI CACGAG 1 cut(s) 287
BbsI GAAGAC 1 cut(s) 407
BbvI GCAGC 4 cut(s) 5, 109, 112, 122
BceAI ACGGC 2 cut(s) 17, 427
BcgI CGANNNNNNTGC 2 cut(s) 35, 69
BcoDI GTCTC 1 cut(s) 152
BfmI CTRYAG 1 cut(s) 136
BisI GCNGC 6 cut(s) 19, 30, 98, 101, 136, 293
BlsI GCNGC 6 cut(s) 20, 31, 99, 102, 137, 294
BmcAI AGTACT 1 cut(s) 422
BmrI ACTGGG 1 cut(s) 285
BmsI GCATC 2 cut(s) 198, 253
BmuI ACTGGG 1 cut(s) 285
BpiI GAAGAC 1 cut(s) 407
BplI GAGNNNNNCTC 2 cut(s) 132, 164
Bpu10I CCTNAGC 1 cut(s) 246
BsaI GGTCTC 1 cut(s) 152
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 545
Bse1I ACTGG 5 cut(s) 195, 280, 337, 428, 496
BseLI CCNNNNNNNGG 2 cut(s) 247, 545
BseMII CTCAG 1 cut(s) 496
BseNI ACTGG 5 cut(s) 195, 280, 337, 428, 496
BsePI GCGCGC 1 cut(s) 323
BseSI GKGCMC 1 cut(s) 375
BseX3I CGGCCG 1 cut(s) 30
BseXI GCAGC 4 cut(s) 5, 109, 112, 122
BseYI CCCAGC 1 cut(s) 79
BsgI GTGCAG 1 cut(s) 116
Bsh1236I CGCG 1 cut(s) 325
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 1 cut(s) 32
BsiEI CGRYCG 1 cut(s) 33
BslI CCNNNNNNNGG 2 cut(s) 247, 545
BsmAI GTCTC 1 cut(s) 152
BsmI GAATGC 1 cut(s) 341
BsnI GGCC 1 cut(s) 32
Bso31I GGTCTC 1 cut(s) 152
Bsp1286I GDGCHC 1 cut(s) 375
BspACI CCGC 3 cut(s) 29, 144, 293
BspANI GGCC 1 cut(s) 32
BspCNI CTCAG 1 cut(s) 495
BspFNI CGCG 1 cut(s) 325
BspMAI CTGCAG 1 cut(s) 140
BspTNI GGTCTC 1 cut(s) 152
BsrI ACTGG 5 cut(s) 195, 280, 337, 428, 496
BssHII GCGCGC 1 cut(s) 323
BssSI CACGAG 1 cut(s) 287
Bst2BI CACGAG 1 cut(s) 287
Bst4CI ACNGT 1 cut(s) 234
BstAPI GCANNNNNTGC 1 cut(s) 158
BstC8I GCNNGC 3 cut(s) 325, 375, 497
BstDEI CTNAG 2 cut(s) 246, 482
BstEII GGTNACC 1 cut(s) 548
BstENI CCTNNNNNAGG 1 cut(s) 245
BstFNI CGCG 1 cut(s) 325
BstHHI GCGC 3 cut(s) 23, 325, 327
BstMAI GTCTC 1 cut(s) 152
BstMCI CGRYCG 1 cut(s) 33
BstMWI GCNNNNNNNGC 4 cut(s) 29, 35, 141, 158
BstPI GGTNACC 1 cut(s) 548
BstSFI CTRYAG 1 cut(s) 136
BstSLI GKGCMC 1 cut(s) 375
BstUI CGCG 1 cut(s) 325
BstV1I GCAGC 4 cut(s) 5, 109, 112, 122
BstV2I GAAGAC 1 cut(s) 407
BstZI CGGCCG 1 cut(s) 30
BsuRI GGCC 1 cut(s) 32
BtsIMutI CAGTG 5 cut(s) 202, 239, 273, 330, 352
Cac8I GCNNGC 3 cut(s) 325, 375, 497
CaiI CAGNNNCTG 1 cut(s) 135
CfoI GCGC 3 cut(s) 23, 325, 327
Csp6I GTAC 1 cut(s) 421
CviJI RGCY 6 cut(s) 32, 83, 103, 135, 313, 499
CviKI_1 RGCY 6 cut(s) 32, 83, 103, 135, 313, 499
CviQI GTAC 1 cut(s) 421
DdeI CTNAG 2 cut(s) 246, 482
DraIII CACNNNGTG 1 cut(s) 379
EaeI YGGCCR 1 cut(s) 30
EagI CGGCCG 1 cut(s) 30
EclXI CGGCCG 1 cut(s) 30
Eco31I GGTCTC 1 cut(s) 152
Eco52I CGGCCG 1 cut(s) 30
Eco91I GGTNACC 1 cut(s) 548
EcoNI CCTNNNNNAGG 1 cut(s) 245
EcoO65I GGTNACC 1 cut(s) 548
EcoRI GAATTC 2 cut(s) 436, 477
FaiI YATR 7 cut(s) 126, 159, 173, 214, 216, 471, 518
Fnu4HI GCNGC 6 cut(s) 19, 30, 98, 101, 136, 293
Fsp4HI GCNGC 6 cut(s) 19, 30, 98, 101, 136, 293
FspI TGCGCA 1 cut(s) 22
GlaI GCGC 3 cut(s) 22, 324, 326
GluI GCNGC 6 cut(s) 19, 30, 98, 101, 136, 293
GsaI CCCAGC 1 cut(s) 83
HaeIII GGCC 1 cut(s) 32
HhaI GCGC 3 cut(s) 23, 325, 327
Hin6I GCGC 3 cut(s) 21, 323, 325
HinP1I GCGC 3 cut(s) 21, 323, 325
HinfI GANTC 2 cut(s) 42, 221
HphI GGTGA 2 cut(s) 184, 458
Hpy166II GTNNAC 1 cut(s) 282
Hpy188I TCNGA 2 cut(s) 226, 485
Hpy188III TCNNGA 2 cut(s) 64, 539
Hpy8I GTNNAC 1 cut(s) 282
HpyAV CCTTC 5 cut(s) 221, 313, 455, 497, 540
HpyCH4III ACNGT 1 cut(s) 234
HpyCH4V TGCA 5 cut(s) 4, 56, 97, 138, 266
HpyF10VI GCNNNNNNNGC 4 cut(s) 29, 35, 141, 158
HpyF3I CTNAG 2 cut(s) 246, 482
HspAI GCGC 3 cut(s) 21, 323, 325
LmnI GCTCC 1 cut(s) 338
LpnPI CCDG 9 cut(s) 24, 49, 93, 208, 261, 318, 409, 477, 513
Lsp1109I GCAGC 4 cut(s) 5, 109, 112, 122
LweI GCATC 2 cut(s) 198, 253
MaeIII GTNAC 2 cut(s) 379, 548
MboII GAAGA 1 cut(s) 412
MhlI GDGCHC 1 cut(s) 375
MluCI AATT 5 cut(s) 205, 257, 428, 436, 477
MlyI GAGTC 1 cut(s) 51
MmeI TCCRAC 1 cut(s) 370
MnlI CCTC 6 cut(s) 125, 134, 179, 251, 458, 479
MseI TTAA 1 cut(s) 417
Mva1269I GAATGC 1 cut(s) 341
MvnI CGCG 1 cut(s) 325
MwoI GCNNNNNNNGC 4 cut(s) 29, 35, 141, 158
NmuCI GTSAC 2 cut(s) 379, 548
NsbI TGCGCA 1 cut(s) 22
PauI GCGCGC 1 cut(s) 323
PctI GAATGC 1 cut(s) 341
PfeI GAWTC 1 cut(s) 221
PkrI GCNGC 6 cut(s) 20, 31, 99, 102, 137, 294
PleI GAGTC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 50
PspEI GGTNACC 1 cut(s) 548
PspFI CCCAGC 1 cut(s) 79
PstI CTGCAG 1 cut(s) 140
PstNI CAGNNNCTG 1 cut(s) 135
PteI GCGCGC 1 cut(s) 323
RsaI GTAC 1 cut(s) 422
RsaNI GTAC 1 cut(s) 421
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 6 cut(s) 19, 30, 98, 101, 136, 293
ScaI AGTACT 1 cut(s) 422
SchI GAGTC 1 cut(s) 51
SduI GDGCHC 1 cut(s) 375
SetI ASST 7 cut(s) 13, 85, 105, 243, 315, 508, 532
SfaNI GCATC 2 cut(s) 198, 253
SfcI CTRYAG 1 cut(s) 136
Sse9I AATT 5 cut(s) 205, 257, 428, 436, 477
SsiI CCGC 3 cut(s) 29, 144, 293
SspI AATATT 1 cut(s) 523
TaaI ACNGT 1 cut(s) 234
TaqI TCGA 2 cut(s) 45, 85
TasI AATT 5 cut(s) 205, 257, 428, 436, 477
TatI WGTACW 1 cut(s) 420
TauI GCSGC 2 cut(s) 32, 295
TfiI GAWTC 1 cut(s) 221
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TscAI CASTG 5 cut(s) 202, 239, 280, 337, 352
TseFI GTSAC 2 cut(s) 379, 548
TseI GCWGC 4 cut(s) 18, 97, 100, 135
Tsp45I GTSAC 2 cut(s) 379, 548
TspDTI ATGAA 2 cut(s) 332, 449
TspGWI ACGGA 3 cut(s) 82, 198, 377
TspRI CASTG 5 cut(s) 202, 239, 280, 337, 352
XagI CCTNNNNNAGG 1 cut(s) 245
XapI RAATTY 2 cut(s) 436, 477
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
ZrmI AGTACT 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.