Rroxscaffold_2G00093750

F-box Kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004848
Physical Location & Seq
Forward (+)
15130303 .. 15132364
2062 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00093750.1

Sequence Viewer

Length: 762 bp
ATGAATTATGGCAAGTCGAAGTTGAACTTCGGCGAGGGGAAAGAGATTGTCTTGTTTGAACCCACGACGCCGGCTTCGAGTCTTTTAGAGAATGGACTGATGGTGGATTTGAATAGGAAAGGAAATTGCAAGAGGTTGAATTTGATAAGAGATGAAATCAGAACAATGATAGGTATAGAGACGCAGTTGTCATATTTGTCTTTAGAGATAGGCGTCTCTTATAGAGATGCAGACACTTTAGGGTTTCTGCGCTATAATCGCCAACAAACTAAGAGGGAAAGTAACAAGGCCTACGGCCAGCGGCAGCCGAAGCGGAAGCGGAAGCAGAAGGGGAAGAAGAAAATAGTCCCCCCCCCCCCCCCCCCGGATGGCGGCGTTGCTGAACGGCCTCACACGCAGCTGTCATCAGTAGATAACATCCCTAAGGTATATTGTGCCGGTGGAGTCCATAAACCTGAATCGAAGGTTACAAATTACAGTGAGCCTGATTTGAAGGTTGCAAGCACAAAGCTTGATGTACCAAGATGGTGCTCTGCTGACGTATTTTCCCTTAGCCTATTGTTTTATGGCTTTGGTTATGATTCTGCTATCGAAGATTACAAGGTCTTAATGGGCAGTGAATCTAAATCTGCATGTGGTTCCCTTACGACCAAAGTTTCCGTTTTTACTCTGAAAACGGGTTCATGGAGGATACTTGAAGGTCTCGAGCATGTTAGCTTGACAGAGAGTGGGTGCATGCTTATTAAACGGTGCTCTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1

Protein Analysis

253

Amino Acids

28.19

Weight (kDa)

9.39

Isoelectric Point (pI)

40.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 189 - 245 6.5e-06 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 187
AciI CCGC 4 cut(s) 301, 313, 319, 372
AcoI YGGCCR 1 cut(s) 295
AcsI RAATTY 1 cut(s) 139
AcyI GRCGYC 2 cut(s) 68, 213
AfaI GTAC 1 cut(s) 519
AfiI CCNNNNNNNGG 3 cut(s) 364, 368, 371
AgsI TTSAA 6 cut(s) 25, 59, 112, 139, 493, 698
AluBI AGCT 3 cut(s) 400, 511, 717
AluI AGCT 3 cut(s) 400, 511, 717
Alw21I GWGCWC 2 cut(s) 533, 755
Alw26I GTCTC 3 cut(s) 173, 220, 707
Ama87I CYCGRG 1 cut(s) 704
AoxI GGCC 3 cut(s) 288, 295, 386
ApeKI GCWGC 2 cut(s) 304, 397
ApoI RAATTY 1 cut(s) 139
ArsI GACNNNNNNTTYG 2 cut(s) 58, 90
AspLEI GCGC 1 cut(s) 252
AsuC2I CCSGG 1 cut(s) 365
AvaI CYCGRG 1 cut(s) 704
AxyI CCTNAGG 1 cut(s) 423
Bbv12I GWGCWC 2 cut(s) 533, 755
BbvI GCAGC 2 cut(s) 316, 409
BccI CCATC 3 cut(s) 94, 362, 519
BceAI ACGGC 2 cut(s) 310, 401
BciVI GTATCC 1 cut(s) 684
BcnI CCSGG 1 cut(s) 365
BcoDI GTCTC 3 cut(s) 173, 220, 707
BfuI GTATCC 1 cut(s) 684
BisI GCNGC 4 cut(s) 302, 305, 373, 398
BlsI GCNGC 4 cut(s) 303, 306, 374, 399
Bme1390I CCNGG 1 cut(s) 365
BmeT110I CYCGRG 1 cut(s) 704
BmiI GGNNCC 1 cut(s) 640
BmrFI CCNGG 1 cut(s) 365
BmsI GCATC 1 cut(s) 217
Bpu10I CCTNAGC 1 cut(s) 551
BpuMI CCSGG 1 cut(s) 365
BsaHI GRCGYC 2 cut(s) 68, 213
BsaI GGTCTC 1 cut(s) 707
BsaJI CCNNGG 1 cut(s) 363
Bsc4I CCNNNNNNNGG 3 cut(s) 364, 368, 371
Bse118I RCCGGY 2 cut(s) 70, 437
Bse21I CCTNAGG 1 cut(s) 423
BseDI CCNNGG 1 cut(s) 363
BseGI GGATG 2 cut(s) 373, 417
BseLI CCNNNNNNNGG 3 cut(s) 364, 368, 371
BseXI GCAGC 2 cut(s) 316, 409
BshFI GGCC 3 cut(s) 290, 297, 388
BsiHKAI GWGCWC 2 cut(s) 533, 755
BsiHKCI CYCGRG 1 cut(s) 704
BsiSI CCGG 3 cut(s) 71, 365, 438
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 3 cut(s) 364, 368, 371
BsmAI GTCTC 3 cut(s) 173, 220, 707
BsmBI CGTCTC 2 cut(s) 173, 220
BsmFI GGGAC 1 cut(s) 332
BsnI GGCC 3 cut(s) 290, 297, 388
Bso31I GGTCTC 1 cut(s) 707
BsoBI CYCGRG 1 cut(s) 704
Bsp1286I GDGCHC 2 cut(s) 533, 755
BspACI CCGC 4 cut(s) 301, 313, 319, 372
BspANI GGCC 3 cut(s) 290, 297, 388
BspLI GGNNCC 1 cut(s) 640
BspTNI GGTCTC 1 cut(s) 707
BsrFI RCCGGY 2 cut(s) 70, 437
BssAI RCCGGY 2 cut(s) 70, 437
BssECI CCNNGG 1 cut(s) 363
BssNI GRCGYC 2 cut(s) 68, 213
Bst4CI ACNGT 2 cut(s) 479, 750
BstACI GRCGYC 2 cut(s) 68, 213
BstC8I GCNNGC 4 cut(s) 72, 299, 502, 737
BstDEI CTNAG 3 cut(s) 270, 423, 551
BstF5I GGATG 2 cut(s) 373, 417
BstHHI GCGC 1 cut(s) 252
BstMAI GTCTC 3 cut(s) 173, 220, 707
BstMWI GCNNNNNNNGC 3 cut(s) 258, 310, 394
BstNSI RCATGY 3 cut(s) 636, 713, 739
BstSCI CCNGG 1 cut(s) 363
BstV1I GCAGC 2 cut(s) 316, 409
Bsu36I CCTNAGG 1 cut(s) 423
BsuI GTATCC 1 cut(s) 684
BsuRI GGCC 3 cut(s) 290, 297, 388
BtsCI GGATG 2 cut(s) 373, 417
BtsI GCAGTG 1 cut(s) 622
BtsIMutI CAGTG 2 cut(s) 484, 622
Cac8I GCNNGC 4 cut(s) 72, 299, 502, 737
CfoI GCGC 1 cut(s) 252
Cfr10I RCCGGY 2 cut(s) 70, 437
CseI GACGC 3 cut(s) 76, 190, 202
Csp6I GTAC 1 cut(s) 518
CviAII CATG 4 cut(s) 633, 684, 710, 736
CviQI GTAC 1 cut(s) 518
DdeI CTNAG 3 cut(s) 270, 423, 551
DrdI GACNNNNNNGTC 1 cut(s) 187
DseDI GACNNNNNNGTC 1 cut(s) 187
EaeI YGGCCR 1 cut(s) 295
Eco147I AGGCCT 1 cut(s) 290
Eco31I GGTCTC 1 cut(s) 707
Eco81I CCTNAGG 1 cut(s) 423
Eco88I CYCGRG 1 cut(s) 704
Esp3I CGTCTC 2 cut(s) 173, 220
FaeI CATG 4 cut(s) 636, 687, 713, 739
FalI AAGNNNNNCTT 2 cut(s) 11, 43
FaqI GGGAC 1 cut(s) 332
FatI CATG 4 cut(s) 632, 683, 709, 735
Fnu4HI GCNGC 4 cut(s) 302, 305, 373, 398
FokI GGATG 2 cut(s) 380, 404
Fsp4HI GCNGC 4 cut(s) 302, 305, 373, 398
GlaI GCGC 1 cut(s) 251
GluI GCNGC 4 cut(s) 302, 305, 373, 398
HaeIII GGCC 3 cut(s) 290, 297, 388
HapII CCGG 3 cut(s) 71, 365, 438
HgaI GACGC 3 cut(s) 76, 190, 202
HhaI GCGC 1 cut(s) 252
Hin1I GRCGYC 2 cut(s) 68, 213
Hin1II CATG 4 cut(s) 636, 687, 713, 739
Hin6I GCGC 1 cut(s) 250
HinP1I GCGC 1 cut(s) 250
HindIII AAGCTT 1 cut(s) 509
HinfI GANTC 5 cut(s) 79, 444, 458, 581, 620
HpaII CCGG 3 cut(s) 71, 365, 438
Hpy188I TCNGA 2 cut(s) 161, 672
Hpy188III TCNNGA 1 cut(s) 704
Hpy99I CGWCG 1 cut(s) 70
HpyAV CCTTC 4 cut(s) 322, 457, 487, 692
HpyCH4III ACNGT 2 cut(s) 479, 750
HpyCH4IV ACGT 1 cut(s) 540
HpyCH4V TGCA 5 cut(s) 129, 230, 500, 632, 735
HpyF10VI GCNNNNNNNGC 3 cut(s) 258, 310, 394
HpyF3I CTNAG 3 cut(s) 270, 423, 551
HpySE526I ACGT 1 cut(s) 540
Hsp92I GRCGYC 2 cut(s) 68, 213
Hsp92II CATG 4 cut(s) 636, 687, 713, 739
HspAI GCGC 1 cut(s) 250
KroI GCCGGC 1 cut(s) 70
KroNI GCCGGC 1 cut(s) 72
LpnPI CCDG 6 cut(s) 84, 311, 378, 451, 468, 498
Lsp1109I GCAGC 2 cut(s) 316, 409
LweI GCATC 1 cut(s) 217
MaeII ACGT 1 cut(s) 540
MaeIII GTNAC 2 cut(s) 281, 466
MboII GAAGA 3 cut(s) 346, 349, 605
MhlI GDGCHC 2 cut(s) 533, 755
MluCI AATT 4 cut(s) 4, 124, 139, 472
MlyI GAGTC 2 cut(s) 88, 453
MnlI CCTC 5 cut(s) 28, 126, 267, 399, 681
MroNI GCCGGC 1 cut(s) 70
MseI TTAA 2 cut(s) 608, 744
MspA1I CMGCKG 2 cut(s) 301, 400
MspI CCGG 3 cut(s) 71, 365, 438
MspR9I CCNGG 1 cut(s) 365
MwoI GCNNNNNNNGC 3 cut(s) 258, 310, 394
NaeI GCCGGC 1 cut(s) 72
NciI CCSGG 1 cut(s) 365
NgoMIV GCCGGC 1 cut(s) 70
NlaIII CATG 4 cut(s) 636, 687, 713, 739
NlaIV GGNNCC 1 cut(s) 640
NspI RCATGY 3 cut(s) 636, 713, 739
PaeI GCATGC 1 cut(s) 739
PaeR7I CTCGAG 1 cut(s) 704
PceI AGGCCT 1 cut(s) 290
PcsI WCGNNNNNNNCGW 1 cut(s) 74
PdiI GCCGGC 1 cut(s) 72
PfeI GAWTC 3 cut(s) 458, 581, 620
PkrI GCNGC 4 cut(s) 303, 306, 374, 399
PleI GAGTC 2 cut(s) 87, 452
PpsI GAGTC 2 cut(s) 87, 452
PspN4I GGNNCC 1 cut(s) 640
PvuII CAGCTG 1 cut(s) 400
RsaI GTAC 1 cut(s) 519
RsaNI GTAC 1 cut(s) 518
SaqAI TTAA 2 cut(s) 608, 744
SatI GCNGC 4 cut(s) 302, 305, 373, 398
SchI GAGTC 2 cut(s) 88, 453
ScrFI CCNGG 1 cut(s) 365
SduI GDGCHC 2 cut(s) 533, 755
SfaNI GCATC 1 cut(s) 217
Sfr274I CTCGAG 1 cut(s) 704
SlaI CTCGAG 1 cut(s) 704
SmlI CTYRAG 1 cut(s) 704
SmoI CTYRAG 1 cut(s) 704
SphI GCATGC 1 cut(s) 739
Sse9I AATT 4 cut(s) 4, 124, 139, 472
SseBI AGGCCT 1 cut(s) 290
SsiI CCGC 4 cut(s) 301, 313, 319, 372
StuI AGGCCT 1 cut(s) 290
StyD4I CCNGG 1 cut(s) 363
TaaI ACNGT 2 cut(s) 479, 750
TaiI ACGT 1 cut(s) 543
TaqI TCGA 5 cut(s) 17, 77, 461, 591, 705
TasI AATT 4 cut(s) 4, 124, 139, 472
TauI GCSGC 2 cut(s) 304, 375
TfiI GAWTC 3 cut(s) 458, 581, 620
Tru1I TTAA 2 cut(s) 608, 744
Tru9I TTAA 2 cut(s) 608, 744
TscAI CASTG 2 cut(s) 484, 622
TseI GCWGC 2 cut(s) 304, 397
TspDTI ATGAA 3 cut(s) 17, 168, 672
TspGWI ACGGA 1 cut(s) 649
TspRI CASTG 2 cut(s) 484, 622
XapI RAATTY 1 cut(s) 139
XceI RCATGY 3 cut(s) 636, 713, 739
XhoI CTCGAG 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.