Rroxscaffold_2G00118530

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002551
Physical Location & Seq
Reverse (-)
50035735 .. 50039407
3673 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00118530.1

Sequence Viewer

Length: 591 bp
ATGAGGTTTTATACTGCTGAGATAGTATCTGCTGTTTCAGATCCTCACAAGTGCGGGATTGTGAATAGTAAACTTAAGCTGGAAAATGTTATCATGGATGTTGATGGGCACAAATGGCTATGCTCATTCGCTTGCTTCGTTGCAACTATTGCTGTGGATGCCTTATCAGTTTGTTCAGACCTAGAGAATAGCTTGGAGAAGATCAAGCGCCGCTTGCTTCCGGTTTCCGTTGGAACTTGCTCCGGGCCCACTCCTCAAGTGATCCAAGACCGTACAAACAAAGACTCTAAGCAACAGAGTAGCCACTTTATTTTCAGGGAGAGGAGACTAGAGTTGTTTGCAAATTGGAGAGCTAAGGGTGGTGTTTTCTTGATTGGCTACTCCATTTTCAGAAACTTATCCCTTGGAACTTTACAAAGTATTCAAATCTACGACAAAAAAGAATTTGCTGATAGCGGTCAGCCTTTACATGAAGCAGCAGTTAATCTAGATGATGTGACTATCTGTTTGCCACAGGATGGGTATAAGGATGTACTGAAGTTAGCTGATAACTTTGGGTCATTATTGCCCACATATCTCTGTAAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chorein_N PH Pex24p SHR-BD VPS13 VPS13_C VPS13_mid_rpt Vps62

Protein Analysis

196

Amino Acids

21.89

Weight (kDa)

7.57

Isoelectric Point (pI)

35.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 518
AciI CCGC 3 cut(s) 54, 211, 456
AclWI GGATC 2 cut(s) 35, 256
AcsI RAATTY 1 cut(s) 443
AcuI CTGAAG 1 cut(s) 557
AfaI GTAC 2 cut(s) 274, 534
AfiI CCNNNNNNNGG 1 cut(s) 518
AflII CTTAAG 1 cut(s) 74
AgsI TTSAA 1 cut(s) 425
AluBI AGCT 4 cut(s) 79, 192, 353, 545
AluI AGCT 4 cut(s) 79, 192, 353, 545
Alw26I GTCTC 1 cut(s) 319
AlwI GGATC 2 cut(s) 35, 256
AoxI GGCC 1 cut(s) 245
ApaI GGGCCC 1 cut(s) 249
ApeKI GCWGC 1 cut(s) 476
ApoI RAATTY 1 cut(s) 443
ArsI GACNNNNNNTTYG 2 cut(s) 490, 522
AspLEI GCGC 1 cut(s) 210
AspS9I GGNCC 2 cut(s) 245, 246
AsuC2I CCSGG 1 cut(s) 244
BaeGI GKGCMC 2 cut(s) 111, 249
BanII GRGCYC 1 cut(s) 249
BbvI GCAGC 1 cut(s) 488
BccI CCATC 2 cut(s) 98, 512
BcnI CCSGG 1 cut(s) 244
BcoDI GTCTC 1 cut(s) 319
BfaI CTAG 3 cut(s) 182, 329, 488
BfoI RGCGCY 1 cut(s) 211
BfrI CTTAAG 1 cut(s) 74
BisI GCNGC 2 cut(s) 211, 477
BlsI GCNGC 2 cut(s) 212, 478
Bme1390I CCNGG 1 cut(s) 244
BmgT120I GGNCC 2 cut(s) 245, 246
BmiI GGNNCC 1 cut(s) 247
BmrFI CCNGG 1 cut(s) 244
BmsI GCATC 1 cut(s) 148
Bpu10I CCTNAGC 1 cut(s) 354
BpuEI CTTGAG 1 cut(s) 240
BpuMI CCSGG 1 cut(s) 244
BsaJI CCNNGG 1 cut(s) 403
BsaWI WCCGGW 1 cut(s) 220
Bsc4I CCNNNNNNNGG 1 cut(s) 518
BseDI CCNNGG 1 cut(s) 403
BseGI GGATG 4 cut(s) 103, 163, 523, 535
BseLI CCNNNNNNNGG 1 cut(s) 518
BseMII CTCAG 1 cut(s) 9
BseRI GAGGAG 2 cut(s) 243, 337
BseSI GKGCMC 2 cut(s) 111, 249
BseXI GCAGC 1 cut(s) 488
BshFI GGCC 1 cut(s) 247
BsiSI CCGG 2 cut(s) 221, 243
BslI CCNNNNNNNGG 1 cut(s) 518
BsmAI GTCTC 1 cut(s) 319
BsnI GGCC 1 cut(s) 247
Bsp120I GGGCCC 1 cut(s) 245
Bsp1286I GDGCHC 2 cut(s) 111, 249
Bsp143I GATC 3 cut(s) 40, 201, 261
BspACI CCGC 3 cut(s) 54, 211, 456
BspANI GGCC 1 cut(s) 247
BspCNI CTCAG 1 cut(s) 10
BspLI GGNNCC 1 cut(s) 247
BspPI GGATC 2 cut(s) 35, 256
BspTI CTTAAG 1 cut(s) 74
BssECI CCNNGG 1 cut(s) 403
BssMI GATC 3 cut(s) 40, 201, 261
BssT1I CCWWGG 1 cut(s) 403
Bst4CI ACNGT 1 cut(s) 272
BstAFI CTTAAG 1 cut(s) 74
BstAPI GCANNNNNTGC 1 cut(s) 149
BstC8I GCNNGC 2 cut(s) 133, 215
BstDEI CTNAG 3 cut(s) 18, 288, 354
BstF5I GGATG 4 cut(s) 103, 163, 523, 535
BstH2I RGCGCY 1 cut(s) 211
BstHHI GCGC 1 cut(s) 210
BstKTI GATC 3 cut(s) 43, 204, 264
BstMAI GTCTC 1 cut(s) 319
BstMBI GATC 3 cut(s) 40, 201, 261
BstMWI GCNNNNNNNGC 4 cut(s) 115, 149, 158, 214
BstSCI CCNGG 1 cut(s) 242
BstSLI GKGCMC 2 cut(s) 111, 249
BstV1I GCAGC 1 cut(s) 488
BstX2I RGATCY 1 cut(s) 40
BstYI RGATCY 1 cut(s) 40
BsuRI GGCC 1 cut(s) 247
BtsCI GGATG 4 cut(s) 103, 163, 523, 535
Cac8I GCNNGC 2 cut(s) 133, 215
CfoI GCGC 1 cut(s) 210
Cfr13I GGNCC 2 cut(s) 245, 246
Csp6I GTAC 2 cut(s) 273, 533
CviAII CATG 2 cut(s) 94, 470
CviJI RGCY 9 cut(s) 79, 118, 192, 247, 303, 353, 378, 463, 545
CviKI_1 RGCY 9 cut(s) 79, 118, 192, 247, 303, 353, 378, 463, 545
CviQI GTAC 2 cut(s) 273, 533
DdeI CTNAG 3 cut(s) 18, 288, 354
DpnI GATC 3 cut(s) 42, 203, 263
DpnII GATC 3 cut(s) 40, 201, 261
Eco130I CCWWGG 1 cut(s) 403
Eco24I GRGCYC 1 cut(s) 249
Eco57I CTGAAG 1 cut(s) 557
EcoT14I CCWWGG 1 cut(s) 403
EcoT38I GRGCYC 1 cut(s) 249
ErhI CCWWGG 1 cut(s) 403
FaeI CATG 2 cut(s) 97, 473
FaiI YATR 6 cut(s) 12, 95, 121, 471, 525, 574
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FatI CATG 2 cut(s) 93, 469
FauI CCCGC 1 cut(s) 47
Fnu4HI GCNGC 2 cut(s) 211, 477
FokI GGATG 4 cut(s) 110, 170, 530, 542
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 2 cut(s) 211, 477
FspBI CTAG 3 cut(s) 182, 329, 488
GlaI GCGC 1 cut(s) 209
GluI GCNGC 2 cut(s) 211, 477
HaeII RGCGCY 1 cut(s) 211
HaeIII GGCC 1 cut(s) 247
HapII CCGG 2 cut(s) 221, 243
HhaI GCGC 1 cut(s) 210
Hin1II CATG 2 cut(s) 97, 473
Hin6I GCGC 1 cut(s) 208
HinP1I GCGC 1 cut(s) 208
HinfI GANTC 1 cut(s) 284
HpaII CCGG 2 cut(s) 221, 243
Hpy166II GTNNAC 2 cut(s) 71, 584
Hpy188I TCNGA 4 cut(s) 40, 178, 392, 590
Hpy188III TCNNGA 2 cut(s) 370, 488
Hpy8I GTNNAC 2 cut(s) 71, 584
HpyCH4III ACNGT 1 cut(s) 272
HpyCH4V TGCA 2 cut(s) 143, 341
HpyF10VI GCNNNNNNNGC 4 cut(s) 115, 149, 158, 214
HpyF3I CTNAG 3 cut(s) 18, 288, 354
Hsp92II CATG 2 cut(s) 97, 473
HspAI GCGC 1 cut(s) 208
Kzo9I GATC 3 cut(s) 40, 201, 261
LmnI GCTCC 1 cut(s) 245
LpnPI CCDG 5 cut(s) 65, 234, 256, 301, 500
Lsp1109I GCAGC 1 cut(s) 488
LweI GCATC 1 cut(s) 148
MaeI CTAG 3 cut(s) 182, 329, 488
MaeIII GTNAC 1 cut(s) 496
MalI GATC 3 cut(s) 42, 203, 263
MboI GATC 3 cut(s) 40, 201, 261
MboII GAAGA 1 cut(s) 211
MflI RGATCY 1 cut(s) 40
MhlI GDGCHC 2 cut(s) 111, 249
MluCI AATT 2 cut(s) 343, 443
MlyI GAGTC 1 cut(s) 278
MmeI TCCRAC 1 cut(s) 211
MnlI CCTC 3 cut(s) 54, 264, 315
MseI TTAA 2 cut(s) 75, 483
MspCI CTTAAG 1 cut(s) 74
MspI CCGG 2 cut(s) 221, 243
MspR9I CCNGG 1 cut(s) 244
MwoI GCNNNNNNNGC 4 cut(s) 115, 149, 158, 214
NciI CCSGG 1 cut(s) 244
NdeII GATC 3 cut(s) 40, 201, 261
NlaIII CATG 2 cut(s) 97, 473
NlaIV GGNNCC 1 cut(s) 247
NmuCI GTSAC 1 cut(s) 496
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PflMI CCANNNNNTGG 1 cut(s) 518
PkrI GCNGC 2 cut(s) 212, 478
PleI GAGTC 1 cut(s) 278
PpsI GAGTC 1 cut(s) 278
PspN4I GGNNCC 1 cut(s) 247
PspOMI GGGCCC 1 cut(s) 245
PspPI GGNCC 2 cut(s) 245, 246
PsuI RGATCY 1 cut(s) 40
RsaI GTAC 2 cut(s) 274, 534
RsaNI GTAC 2 cut(s) 273, 533
SaqAI TTAA 2 cut(s) 75, 483
SatI GCNGC 2 cut(s) 211, 477
Sau3AI GATC 3 cut(s) 40, 201, 261
Sau96I GGNCC 2 cut(s) 245, 246
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 1 cut(s) 244
SduI GDGCHC 2 cut(s) 111, 249
SetI ASST 6 cut(s) 8, 81, 183, 194, 355, 547
SfaNI GCATC 1 cut(s) 148
SmlI CTYRAG 2 cut(s) 74, 255
SmoI CTYRAG 2 cut(s) 74, 255
Sse9I AATT 2 cut(s) 343, 443
SsiI CCGC 3 cut(s) 54, 211, 456
SspMI CTAG 3 cut(s) 182, 329, 488
StyD4I CCNGG 1 cut(s) 242
StyI CCWWGG 1 cut(s) 403
TaaI ACNGT 1 cut(s) 272
TasI AATT 2 cut(s) 343, 443
TatI WGTACW 1 cut(s) 532
TauI GCSGC 1 cut(s) 213
Tru1I TTAA 2 cut(s) 75, 483
Tru9I TTAA 2 cut(s) 75, 483
TseFI GTSAC 1 cut(s) 496
TseI GCWGC 1 cut(s) 476
Tsp45I GTSAC 1 cut(s) 496
TspDTI ATGAA 1 cut(s) 486
TspGWI ACGGA 1 cut(s) 217
Van91I CCANNNNNTGG 1 cut(s) 518
Vha464I CTTAAG 1 cut(s) 74
XapI RAATTY 1 cut(s) 443
XbaI TCTAGA 1 cut(s) 487
XspI CTAG 3 cut(s) 182, 329, 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.