Rroxscaffold_4G00311990

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002551
Physical Location & Seq
Reverse (-)
34664631 .. 34683849
19219 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00311990.1

Sequence Viewer

Length: 732 bp
ATGGGAACAGTACATTGCAAAGGAACCTGCCATGGCGTGGCCGGGTTAGTGCTGCATCACGTGCGTGCATGTGCCTATCTAAGTCGTATTTGCGAGTCCGCATACTATGCAGATCTGCACAGCAGCTTAATCCATGAGCACATATCTTTCAAAATGCCCCTCTTTTACATATCAACAAGCCAACATCTCTCTCAAGCTTTTAGCCAGCATTATGGACACACCACGCAGATGAAGGCAATTCGGAATGTTCGGGCTGGTGGATCTCTAGATATCAACTTTGGATCCACAAGTTCTACCGATGATGGTGGGTCCATTGATCTAAACTTTGGTTCCATTGGTTCCATTGGTCTTGATGGATCTATCGATCTAACTTTTGGGTCAAGTGTCGACTATAATGCATGTCGAGCCTTACACAAGCTCATGCCAAGCAATGCCAAGCCCCTCTTTGTCAAGCCTCACCCTCAATTGCCTGCACCTAAACGACTTTTCATTGACGGTTTTTCTGGGCAAGCATCTCCAAGAACTGAACGTGCTGTGCTACTCCTTGATGTCGTATCATGTCCTTGCTTTCATTGGAGAGACTTGCCCCAATCTCAGGGTGCTTGTGCTAGAATTGTAGACCAGGAATCACCTGAGGCTTTTAAGATCAACCTGGACAGAATGCTTAATGGTTGTTTATGCTTGGAGGTATCGAAGAGAGGCTCAAATTATAATTCTGATTCTGTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box LRR_6

Protein Analysis

243

Amino Acids

26.5

Weight (kDa)

7.63

Isoelectric Point (pI)

48.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 711
Acc36I ACCTGC 1 cut(s) 35
AccB7I CCANNNNNTGG 1 cut(s) 37
AccI GTMKAC 2 cut(s) 387, 618
AciI CCGC 1 cut(s) 99
AclWI GGATC 4 cut(s) 268, 276, 289, 364
AcoI YGGCCR 1 cut(s) 39
AcvI CACGTG 1 cut(s) 61
AfaI GTAC 1 cut(s) 12
AfiI CCNNNNNNNGG 2 cut(s) 37, 595
AgsI TTSAA 1 cut(s) 151
AjnI CCWGG 2 cut(s) 621, 651
AleI CACNNNNGTG 1 cut(s) 63
AluBI AGCT 3 cut(s) 126, 197, 418
AluI AGCT 3 cut(s) 126, 197, 418
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 1 cut(s) 573
AlwI GGATC 4 cut(s) 268, 276, 289, 364
AoxI GGCC 1 cut(s) 39
ApeKI GCWGC 2 cut(s) 52, 123
AspS9I GGNCC 1 cut(s) 309
AsuC2I CCSGG 1 cut(s) 43
AsuHPI GGTGA 2 cut(s) 449, 621
AvaII GGWCC 1 cut(s) 309
AxyI CCTNAGG 1 cut(s) 633
BamHI GGATCC 1 cut(s) 281
BbrPI CACGTG 1 cut(s) 61
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 2 cut(s) 39, 135
BccI CCATC 2 cut(s) 296, 347
BcgI CGANNNNNNTGC 2 cut(s) 377, 411
BciT130I CCWGG 2 cut(s) 623, 653
BcnI CCSGG 1 cut(s) 43
BcoDI GTCTC 1 cut(s) 573
BfaI CTAG 2 cut(s) 266, 609
BfuAI ACCTGC 1 cut(s) 35
BglII AGATCT 1 cut(s) 112
BisI GCNGC 2 cut(s) 53, 124
BlsI GCNGC 2 cut(s) 54, 125
Bme1390I CCNGG 3 cut(s) 43, 623, 653
Bme18I GGWCC 1 cut(s) 309
BmgT120I GGNCC 1 cut(s) 309
BmiI GGNNCC 5 cut(s) 25, 283, 310, 331, 340
BmrFI CCNGG 3 cut(s) 43, 623, 653
BmsI GCATC 2 cut(s) 64, 521
BpuEI CTTGAG 1 cut(s) 177
BpuMI CCSGG 1 cut(s) 43
Bsa29I ATCGAT 1 cut(s) 363
BsaAI YACGTR 1 cut(s) 61
BsaJI CCNNGG 1 cut(s) 31
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 595
Bse21I CCTNAGG 1 cut(s) 633
Bse3DI GCAATG 2 cut(s) 13, 436
BseBI CCWGG 2 cut(s) 623, 653
BseCI ATCGAT 1 cut(s) 363
BseDI CCNNGG 1 cut(s) 31
BseLI CCNNNNNNNGG 2 cut(s) 37, 595
BseMI GCAATG 2 cut(s) 13, 436
BseMII CTCAG 2 cut(s) 608, 624
BseXI GCAGC 2 cut(s) 39, 135
BsgI GTGCAG 2 cut(s) 101, 456
BshFI GGCC 1 cut(s) 41
BshVI ATCGAT 1 cut(s) 363
BsiHKAI GWGCWC 1 cut(s) 141
BsiSI CCGG 1 cut(s) 42
BslI CCNNNNNNNGG 2 cut(s) 37, 595
BsmAI GTCTC 1 cut(s) 573
BsmI GAATGC 1 cut(s) 666
BsnI GGCC 1 cut(s) 41
Bsp1286I GDGCHC 1 cut(s) 141
Bsp143I GATC 7 cut(s) 112, 260, 281, 316, 356, 364, 645
Bsp19I CCATGG 1 cut(s) 31
BspACI CCGC 1 cut(s) 99
BspANI GGCC 1 cut(s) 41
BspCNI CTCAG 2 cut(s) 607, 625
BspDI ATCGAT 1 cut(s) 363
BspLI GGNNCC 5 cut(s) 25, 283, 310, 331, 340
BspMI ACCTGC 1 cut(s) 35
BspPI GGATC 4 cut(s) 268, 276, 289, 364
BsrDI GCAATG 2 cut(s) 13, 436
BssECI CCNNGG 1 cut(s) 31
BssMI GATC 7 cut(s) 112, 260, 281, 316, 356, 364, 645
BssT1I CCWWGG 1 cut(s) 31
Bst2UI CCWGG 2 cut(s) 623, 653
Bst4CI ACNGT 2 cut(s) 10, 497
Bst6I CTCTTC 1 cut(s) 689
BstAPI GCANNNNNTGC 2 cut(s) 61, 107
BstBAI YACGTR 1 cut(s) 61
BstC8I GCNNGC 4 cut(s) 66, 206, 471, 510
BstDEI CTNAG 3 cut(s) 80, 594, 633
BstDSI CCRYGG 1 cut(s) 31
BstKTI GATC 7 cut(s) 115, 263, 284, 319, 359, 367, 648
BstMAI GTCTC 1 cut(s) 573
BstMBI GATC 7 cut(s) 112, 260, 281, 316, 356, 364, 645
BstMWI GCNNNNNNNGC 3 cut(s) 61, 107, 404
BstNI CCWGG 2 cut(s) 623, 653
BstNSI RCATGY 2 cut(s) 72, 402
BstSCI CCNGG 3 cut(s) 41, 621, 651
BstV1I GCAGC 2 cut(s) 39, 135
BstX2I RGATCY 4 cut(s) 112, 260, 281, 356
BstXI CCANNNNNNTGG 1 cut(s) 212
BstYI RGATCY 4 cut(s) 112, 260, 281, 356
Bsu15I ATCGAT 1 cut(s) 363
Bsu36I CCTNAGG 1 cut(s) 633
BsuRI GGCC 1 cut(s) 41
BsuTUI ATCGAT 1 cut(s) 363
BtgI CCRYGG 1 cut(s) 31
BveI ACCTGC 1 cut(s) 35
Cac8I GCNNGC 4 cut(s) 66, 206, 471, 510
Cfr13I GGNCC 1 cut(s) 309
ClaI ATCGAT 1 cut(s) 363
Csp6I GTAC 1 cut(s) 11
CviAII CATG 6 cut(s) 32, 69, 134, 399, 421, 558
CviQI GTAC 1 cut(s) 11
DdeI CTNAG 3 cut(s) 80, 594, 633
DpnI GATC 7 cut(s) 114, 262, 283, 318, 358, 366, 647
DpnII GATC 7 cut(s) 112, 260, 281, 316, 356, 364, 645
EaeI YGGCCR 1 cut(s) 39
Eam1104I CTCTTC 1 cut(s) 689
EarI CTCTTC 1 cut(s) 689
Eco130I CCWWGG 1 cut(s) 31
Eco32I GATATC 1 cut(s) 271
Eco47I GGWCC 1 cut(s) 309
Eco72I CACGTG 1 cut(s) 61
Eco81I CCTNAGG 1 cut(s) 633
EcoRII CCWGG 2 cut(s) 621, 651
EcoRV GATATC 1 cut(s) 271
EcoT14I CCWWGG 1 cut(s) 31
EcoT22I ATGCAT 1 cut(s) 400
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 6 cut(s) 35, 72, 137, 402, 424, 561
FalI AAGNNNNNCTT 2 cut(s) 428, 460
FatI CATG 6 cut(s) 31, 68, 133, 398, 420, 557
FblI GTMKAC 2 cut(s) 387, 618
Fnu4HI GCNGC 2 cut(s) 53, 124
Fsp4HI GCNGC 2 cut(s) 53, 124
FspBI CTAG 2 cut(s) 266, 609
GluI GCNGC 2 cut(s) 53, 124
HaeIII GGCC 1 cut(s) 41
HapII CCGG 1 cut(s) 42
Hin1II CATG 6 cut(s) 35, 72, 137, 402, 424, 561
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HindIII AAGCTT 1 cut(s) 195
HinfI GANTC 3 cut(s) 95, 626, 719
HpaII CCGG 1 cut(s) 42
HphI GGTGA 2 cut(s) 449, 621
Hpy166II GTNNAC 2 cut(s) 388, 619
Hpy188I TCNGA 2 cut(s) 243, 718
Hpy188III TCNNGA 2 cut(s) 266, 350
Hpy8I GTNNAC 2 cut(s) 388, 619
HpyAV CCTTC 1 cut(s) 226
HpyCH4III ACNGT 2 cut(s) 10, 497
HpyCH4IV ACGT 2 cut(s) 60, 529
HpyCH4V TGCA 7 cut(s) 18, 55, 68, 110, 118, 398, 473
HpyF10VI GCNNNNNNNGC 3 cut(s) 61, 107, 404
HpyF3I CTNAG 3 cut(s) 80, 594, 633
HpySE526I ACGT 2 cut(s) 60, 529
Hsp92II CATG 6 cut(s) 35, 72, 137, 402, 424, 561
Kzo9I GATC 7 cut(s) 112, 260, 281, 316, 356, 364, 645
Lsp1109I GCAGC 2 cut(s) 39, 135
LweI GCATC 2 cut(s) 64, 521
MaeI CTAG 2 cut(s) 266, 609
MaeII ACGT 2 cut(s) 60, 529
MalI GATC 7 cut(s) 114, 262, 283, 318, 358, 366, 647
MboI GATC 7 cut(s) 112, 260, 281, 316, 356, 364, 645
MboII GAAGA 1 cut(s) 706
MfeI CAATTG 1 cut(s) 464
MflI RGATCY 4 cut(s) 112, 260, 281, 356
MhlI GDGCHC 1 cut(s) 141
MluCI AATT 5 cut(s) 237, 464, 612, 706, 712
MlyI GAGTC 1 cut(s) 104
MnlI CCTC 7 cut(s) 170, 452, 465, 471, 628, 679, 692
Mph1103I ATGCAT 1 cut(s) 400
MseI TTAA 3 cut(s) 128, 642, 666
MslI CAYNNNNRTG 2 cut(s) 63, 227
MspI CCGG 1 cut(s) 42
MspR9I CCNGG 3 cut(s) 43, 623, 653
MunI CAATTG 1 cut(s) 464
Mva1269I GAATGC 1 cut(s) 666
MvaI CCWGG 2 cut(s) 623, 653
MwoI GCNNNNNNNGC 3 cut(s) 61, 107, 404
NciI CCSGG 1 cut(s) 43
NcoI CCATGG 1 cut(s) 31
NdeII GATC 7 cut(s) 112, 260, 281, 316, 356, 364, 645
NlaIII CATG 6 cut(s) 35, 72, 137, 402, 424, 561
NlaIV GGNNCC 5 cut(s) 25, 283, 310, 331, 340
NsiI ATGCAT 1 cut(s) 400
NspI RCATGY 2 cut(s) 72, 402
OliI CACNNNNGTG 1 cut(s) 63
PctI GAATGC 1 cut(s) 666
PfeI GAWTC 2 cut(s) 626, 719
PflMI CCANNNNNTGG 1 cut(s) 37
PkrI GCNGC 2 cut(s) 54, 125
PleI GAGTC 1 cut(s) 103
PmaCI CACGTG 1 cut(s) 61
PmlI CACGTG 1 cut(s) 61
PpsI GAGTC 1 cut(s) 103
Ppu21I YACGTR 1 cut(s) 61
PsiI TTATAA 1 cut(s) 711
Psp6I CCWGG 2 cut(s) 621, 651
PspCI CACGTG 1 cut(s) 61
PspGI CCWGG 2 cut(s) 621, 651
PspN4I GGNNCC 5 cut(s) 25, 283, 310, 331, 340
PspPI GGNCC 1 cut(s) 309
PsuI RGATCY 4 cut(s) 112, 260, 281, 356
RsaI GTAC 1 cut(s) 12
RsaNI GTAC 1 cut(s) 11
RseI CAYNNNNRTG 2 cut(s) 63, 227
SalI GTCGAC 1 cut(s) 386
SaqAI TTAA 3 cut(s) 128, 642, 666
SatI GCNGC 2 cut(s) 53, 124
Sau3AI GATC 7 cut(s) 112, 260, 281, 316, 356, 364, 645
Sau96I GGNCC 1 cut(s) 309
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 3 cut(s) 43, 623, 653
SduI GDGCHC 1 cut(s) 141
SfaNI GCATC 2 cut(s) 64, 521
SinI GGWCC 1 cut(s) 309
SmiMI CAYNNNNRTG 2 cut(s) 63, 227
SmlI CTYRAG 1 cut(s) 192
SmoI CTYRAG 1 cut(s) 192
Sse9I AATT 5 cut(s) 237, 464, 612, 706, 712
SsiI CCGC 1 cut(s) 99
SspMI CTAG 2 cut(s) 266, 609
StyD4I CCNGG 3 cut(s) 41, 621, 651
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 2 cut(s) 10, 497
TaiI ACGT 2 cut(s) 63, 532
TaqI TCGA 4 cut(s) 363, 387, 403, 692
TasI AATT 5 cut(s) 237, 464, 612, 706, 712
TatI WGTACW 1 cut(s) 10
TfiI GAWTC 2 cut(s) 626, 719
Tru1I TTAA 3 cut(s) 128, 642, 666
Tru9I TTAA 3 cut(s) 128, 642, 666
TseI GCWGC 2 cut(s) 52, 123
TspDTI ATGAA 3 cut(s) 245, 478, 560
Van91I CCANNNNNTGG 1 cut(s) 37
VpaK11BI GGWCC 1 cut(s) 309
XbaI TCTAGA 1 cut(s) 265
XceI RCATGY 2 cut(s) 72, 402
XmiI GTMKAC 2 cut(s) 387, 618
XspI CTAG 2 cut(s) 266, 609
Zsp2I ATGCAT 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.