Rh1BG388800

Repeating coiled region of VPS13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0002551
Physical Location & Seq
Reverse (-)
51550489 .. 51552619
2131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG388800.1

Sequence Viewer

Length: 576 bp
ATGAATTTTTGTGAATTCACAGAGAGTTCAGACCTAGAGAATAGCTTGGAGAAGATCAAGCGCCAGTTTGCTTCCGGTTTCCGTTGGAACTTGCTCCAGGGCCCACTCCTCAAGCGATCCAAGACTGTACAAACAAAGACTCTAAGCAACAGATTAGCCACTTTATTTTCAGAAACTTATCCCTTGGAACCTTCTGTCGAACTTGATCGGCTTGCACTTTATCTGGATCATGATATTTGCTTCATCCTCCATGGCATGTTGACAAGCAATGGAAGGATTTGCTTCCATCAGAGTGGGTTCAGGTTTTTTAGATTTGGTACTAAAGTTGGTAAACCAGTGAATAAATTGACCAAGAAGCATACTGATGTATTGGGAGTTTGGGACCTTTTTTTAGAAGTACAAAGTATTCAAATCTACGACAAAAAAGAATTTGCTGATAGCGGTCAGCCTTTACATGAAGCAGTAGTTAATCTAGATGATGTGACTATCTGTTTGCCACAGGACAGGTATAAGGATGTACTGAAGTTAGCTGATAACTTTGGGTCATTATTGCCCACATATCTCTGTAAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chorein_N PH Pex24p SHR-BD VPS13 VPS13_C VPS13_mid_rpt Vps62

Protein Analysis

191

Amino Acids

21.97

Weight (kDa)

7.65

Isoelectric Point (pI)

32.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 441
AclWI GGATC 2 cut(s) 111, 234
AcsI RAATTY 3 cut(s) 4, 14, 428
AcuI CTGAAG 1 cut(s) 542
AfaI GTAC 4 cut(s) 129, 319, 399, 519
AgsI TTSAA 1 cut(s) 410
AjnI CCWGG 1 cut(s) 96
AluBI AGCT 2 cut(s) 45, 530
AluI AGCT 2 cut(s) 45, 530
AlwI GGATC 2 cut(s) 111, 234
AoxI GGCC 1 cut(s) 100
ApaI GGGCCC 1 cut(s) 104
ApoI RAATTY 3 cut(s) 4, 14, 428
ArsI GACNNNNNNTTYG 2 cut(s) 475, 507
AspLEI GCGC 1 cut(s) 63
AspS9I GGNCC 3 cut(s) 100, 101, 382
AvaII GGWCC 1 cut(s) 382
BaeGI GKGCMC 1 cut(s) 104
BaeI ACNNNNGTAYC 2 cut(s) 309, 342
BanII GRGCYC 1 cut(s) 104
BccI CCATC 1 cut(s) 294
BciT130I CCWGG 1 cut(s) 98
BfaI CTAG 2 cut(s) 35, 473
BfoI RGCGCY 1 cut(s) 64
Bme1390I CCNGG 1 cut(s) 98
Bme18I GGWCC 1 cut(s) 382
BmgT120I GGNCC 3 cut(s) 100, 101, 382
BmiI GGNNCC 3 cut(s) 102, 189, 383
BmrFI CCNGG 1 cut(s) 98
BpmI CTGGAG 1 cut(s) 80
BpuEI CTTGAG 1 cut(s) 95
BsaJI CCNNGG 3 cut(s) 97, 183, 250
BsaWI WCCGGW 1 cut(s) 74
Bse1I ACTGG 2 cut(s) 64, 335
Bse3DI GCAATG 1 cut(s) 274
BseBI CCWGG 1 cut(s) 98
BseDI CCNNGG 3 cut(s) 97, 183, 250
BseGI GGATG 2 cut(s) 243, 520
BseMI GCAATG 1 cut(s) 274
BseNI ACTGG 2 cut(s) 64, 335
BseRI GAGGAG 1 cut(s) 98
BseSI GKGCMC 1 cut(s) 104
BshFI GGCC 1 cut(s) 102
BsiSI CCGG 1 cut(s) 75
BslFI GGGAC 1 cut(s) 395
BsmFI GGGAC 1 cut(s) 395
BsnI GGCC 1 cut(s) 102
Bsp120I GGGCCC 1 cut(s) 100
Bsp1286I GDGCHC 1 cut(s) 104
Bsp1407I TGTACA 1 cut(s) 127
Bsp143I GATC 4 cut(s) 54, 116, 205, 226
Bsp19I CCATGG 1 cut(s) 250
BspACI CCGC 1 cut(s) 441
BspANI GGCC 1 cut(s) 102
BspHI TCATGA 1 cut(s) 229
BspLI GGNNCC 3 cut(s) 102, 189, 383
BspPI GGATC 2 cut(s) 111, 234
BsrDI GCAATG 1 cut(s) 274
BsrGI TGTACA 1 cut(s) 127
BsrI ACTGG 2 cut(s) 64, 335
BssECI CCNNGG 3 cut(s) 97, 183, 250
BssMI GATC 4 cut(s) 54, 116, 205, 226
BssT1I CCWWGG 2 cut(s) 183, 250
Bst2UI CCWGG 1 cut(s) 98
Bst4CI ACNGT 1 cut(s) 127
BstAUI TGTACA 1 cut(s) 127
BstC8I GCNNGC 1 cut(s) 213
BstDEI CTNAG 1 cut(s) 143
BstDSI CCRYGG 1 cut(s) 250
BstF5I GGATG 2 cut(s) 243, 520
BstH2I RGCGCY 1 cut(s) 64
BstHHI GCGC 1 cut(s) 63
BstKTI GATC 4 cut(s) 57, 119, 208, 229
BstMBI GATC 4 cut(s) 54, 116, 205, 226
BstNI CCWGG 1 cut(s) 98
BstNSI RCATGY 1 cut(s) 259
BstSCI CCNGG 1 cut(s) 96
BstSLI GKGCMC 1 cut(s) 104
BstXI CCANNNNNNTGG 1 cut(s) 293
BsuRI GGCC 1 cut(s) 102
BtgI CCRYGG 1 cut(s) 250
BtsCI GGATG 2 cut(s) 243, 520
BtsIMutI CAGTG 1 cut(s) 342
Cac8I GCNNGC 1 cut(s) 213
CciI TCATGA 1 cut(s) 229
CfoI GCGC 1 cut(s) 63
Cfr13I GGNCC 3 cut(s) 100, 101, 382
Csp6I GTAC 4 cut(s) 128, 318, 398, 518
CviAII CATG 4 cut(s) 230, 251, 256, 455
CviJI RGCY 6 cut(s) 45, 102, 158, 211, 448, 530
CviKI_1 RGCY 6 cut(s) 45, 102, 158, 211, 448, 530
CviQI GTAC 4 cut(s) 128, 318, 398, 518
DdeI CTNAG 1 cut(s) 143
DpnI GATC 4 cut(s) 56, 118, 207, 228
DpnII GATC 4 cut(s) 54, 116, 205, 226
Eco130I CCWWGG 2 cut(s) 183, 250
Eco24I GRGCYC 1 cut(s) 104
Eco47I GGWCC 1 cut(s) 382
Eco57I CTGAAG 1 cut(s) 542
EcoO109I RGGNCCY 2 cut(s) 100, 382
EcoRI GAATTC 1 cut(s) 14
EcoRII CCWGG 1 cut(s) 96
EcoT14I CCWWGG 2 cut(s) 183, 250
EcoT38I GRGCYC 1 cut(s) 104
ErhI CCWWGG 2 cut(s) 183, 250
FaeI CATG 4 cut(s) 233, 254, 259, 458
FaiI YATR 7 cut(s) 231, 252, 257, 360, 456, 510, 559
FaqI GGGAC 1 cut(s) 395
FatI CATG 4 cut(s) 229, 250, 255, 454
FokI GGATG 2 cut(s) 230, 527
FriOI GRGCYC 1 cut(s) 104
FspBI CTAG 2 cut(s) 35, 473
GlaI GCGC 1 cut(s) 62
GsuI CTGGAG 1 cut(s) 80
HaeII RGCGCY 1 cut(s) 64
HaeIII GGCC 1 cut(s) 102
HapII CCGG 1 cut(s) 75
HhaI GCGC 1 cut(s) 63
Hin1II CATG 4 cut(s) 233, 254, 259, 458
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HincII GTYRAC 1 cut(s) 261
HindII GTYRAC 1 cut(s) 261
HinfI GANTC 1 cut(s) 139
HpaII CCGG 1 cut(s) 75
Hpy166II GTNNAC 3 cut(s) 261, 332, 569
Hpy188I TCNGA 3 cut(s) 31, 172, 291
Hpy188III TCNNGA 3 cut(s) 224, 230, 473
Hpy8I GTNNAC 3 cut(s) 261, 332, 569
HpyAV CCTTC 2 cut(s) 201, 267
HpyCH4III ACNGT 1 cut(s) 127
HpyCH4V TGCA 1 cut(s) 215
HpyF3I CTNAG 1 cut(s) 143
Hsp92II CATG 4 cut(s) 233, 254, 259, 458
HspAI GCGC 1 cut(s) 61
Kzo9I GATC 4 cut(s) 54, 116, 205, 226
LmnI GCTCC 1 cut(s) 99
LpnPI CCDG 9 cut(s) 77, 83, 88, 110, 209, 286, 348, 485, 490
MaeI CTAG 2 cut(s) 35, 473
MaeIII GTNAC 1 cut(s) 481
MalI GATC 4 cut(s) 56, 118, 207, 228
MboI GATC 4 cut(s) 54, 116, 205, 226
MboII GAAGA 1 cut(s) 64
MhlI GDGCHC 1 cut(s) 104
MluCI AATT 4 cut(s) 4, 14, 344, 428
MlyI GAGTC 1 cut(s) 133
MmeI TCCRAC 1 cut(s) 65
MnlI CCTC 2 cut(s) 119, 257
MseI TTAA 1 cut(s) 468
MslI CAYNNNNRTG 2 cut(s) 291, 363
MspI CCGG 1 cut(s) 75
MspR9I CCNGG 1 cut(s) 98
MvaI CCWGG 1 cut(s) 98
NcoI CCATGG 1 cut(s) 250
NdeII GATC 4 cut(s) 54, 116, 205, 226
NlaIII CATG 4 cut(s) 233, 254, 259, 458
NlaIV GGNNCC 3 cut(s) 102, 189, 383
NmuCI GTSAC 1 cut(s) 481
NspI RCATGY 1 cut(s) 259
PagI TCATGA 1 cut(s) 229
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
PpuMI RGGWCCY 1 cut(s) 382
Psp5II RGGWCCY 1 cut(s) 382
Psp6I CCWGG 1 cut(s) 96
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 3 cut(s) 102, 189, 383
PspOMI GGGCCC 1 cut(s) 100
PspPI GGNCC 3 cut(s) 100, 101, 382
PspPPI RGGWCCY 1 cut(s) 382
RsaI GTAC 4 cut(s) 129, 319, 399, 519
RsaNI GTAC 4 cut(s) 128, 318, 398, 518
RseI CAYNNNNRTG 2 cut(s) 291, 363
SaqAI TTAA 1 cut(s) 468
Sau3AI GATC 4 cut(s) 54, 116, 205, 226
Sau96I GGNCC 3 cut(s) 100, 101, 382
SchI GAGTC 1 cut(s) 133
ScrFI CCNGG 1 cut(s) 98
SduI GDGCHC 1 cut(s) 104
SetI ASST 7 cut(s) 36, 47, 193, 305, 387, 509, 532
SinI GGWCC 1 cut(s) 382
SmiMI CAYNNNNRTG 2 cut(s) 291, 363
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 4 cut(s) 4, 14, 344, 428
SsiI CCGC 1 cut(s) 441
SspMI CTAG 2 cut(s) 35, 473
StyD4I CCNGG 1 cut(s) 96
StyI CCWWGG 2 cut(s) 183, 250
TaaI ACNGT 1 cut(s) 127
TaqI TCGA 1 cut(s) 198
TasI AATT 4 cut(s) 4, 14, 344, 428
TatI WGTACW 3 cut(s) 127, 397, 517
Tru1I TTAA 1 cut(s) 468
Tru9I TTAA 1 cut(s) 468
TscAI CASTG 1 cut(s) 342
TseFI GTSAC 1 cut(s) 481
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 3 cut(s) 17, 232, 471
TspGWI ACGGA 1 cut(s) 71
TspRI CASTG 1 cut(s) 342
VpaK11BI GGWCC 1 cut(s) 382
XapI RAATTY 3 cut(s) 4, 14, 428
XbaI TCTAGA 1 cut(s) 472
XceI RCATGY 1 cut(s) 259
XspI CTAG 2 cut(s) 35, 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.