Rh6BG073700

Repeating coiled region of VPS13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0002551
Physical Location & Seq
Reverse (-)
11168292 .. 11187421
19130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG073700.1

Sequence Viewer

Length: 504 bp
ATGATCATATTCGCTCCACTTAAGAAAAAGAGTTCAGACCTAGAGAATAGCTTGGAGAAGATCAAGCGCCAGTTTGCTTCCGGTTTCCGTTGGAACTTGCTCCAGGGCCCACTCCTCAAGCGATCCAAGACTTCTGTCGAACTTGATCGGCTTGCACTTTATCTGGATTGTGATATTTGCTTCATCCTCCATGGCATGTTGACAAACAATGGAAGGATTTGCTTCCATCAGAGTGGGTTCAGGTTTTTTAGATTTGGTACTAAAGTTGGTAAACCAGTGAATAAATTGACCAAGAAGCATACGTATTGGGACCTTTTTTTAGAAGTACAAAGTATTCAAATCTACGACAAAAAAGAATTTGCTGATAGCGGTCAGCCTTTACATGAAGCAGCAGTTAATCTAGATGATGTGACTATCTGTTTGCCACAGGACGGGTATAAGGATGTACTGAAGTTAGCTGATAACTTTGGGTCATTATTGCCCACATATCTCTGTAAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chorein_N PH Pex24p SHR-BD VPS13 VPS13_C VPS13_mid_rpt Vps62

Protein Analysis

167

Amino Acids

19.19

Weight (kDa)

9.07

Isoelectric Point (pI)

31.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 369
AclWI GGATC 1 cut(s) 117
AcsI RAATTY 1 cut(s) 356
AcuI CTGAAG 1 cut(s) 470
AfaI GTAC 3 cut(s) 259, 327, 447
AfiI CCNNNNNNNGG 1 cut(s) 431
AflII CTTAAG 1 cut(s) 20
AgsI TTSAA 1 cut(s) 338
AjnI CCWGG 1 cut(s) 102
AluBI AGCT 2 cut(s) 51, 458
AluI AGCT 2 cut(s) 51, 458
AlwI GGATC 1 cut(s) 117
AoxI GGCC 1 cut(s) 106
ApaI GGGCCC 1 cut(s) 110
ApeKI GCWGC 1 cut(s) 389
ApoI RAATTY 1 cut(s) 356
ArsI GACNNNNNNTTYG 2 cut(s) 403, 435
AspLEI GCGC 1 cut(s) 69
AspS9I GGNCC 3 cut(s) 106, 107, 310
AvaII GGWCC 1 cut(s) 310
BaeGI GKGCMC 1 cut(s) 110
BaeI ACNNNNGTAYC 2 cut(s) 249, 282
BanII GRGCYC 1 cut(s) 110
BbvI GCAGC 1 cut(s) 401
BccI CCATC 1 cut(s) 234
BciT130I CCWGG 1 cut(s) 104
BclI TGATCA 1 cut(s) 3
BfaI CTAG 2 cut(s) 41, 401
BfoI RGCGCY 1 cut(s) 70
BfrI CTTAAG 1 cut(s) 20
BisI GCNGC 1 cut(s) 390
BlsI GCNGC 1 cut(s) 391
Bme1390I CCNGG 1 cut(s) 104
Bme18I GGWCC 1 cut(s) 310
BmgT120I GGNCC 3 cut(s) 106, 107, 310
BmiI GGNNCC 2 cut(s) 108, 311
BmrFI CCNGG 1 cut(s) 104
BoxI GACNNNNGTC 1 cut(s) 134
BpmI CTGGAG 1 cut(s) 86
BpuEI CTTGAG 1 cut(s) 101
BsaAI YACGTR 1 cut(s) 303
BsaJI CCNNGG 2 cut(s) 103, 190
BsaWI WCCGGW 1 cut(s) 80
Bsc4I CCNNNNNNNGG 1 cut(s) 431
Bse1I ACTGG 2 cut(s) 70, 275
BseBI CCWGG 1 cut(s) 104
BseDI CCNNGG 2 cut(s) 103, 190
BseGI GGATG 2 cut(s) 183, 448
BseLI CCNNNNNNNGG 1 cut(s) 431
BseNI ACTGG 2 cut(s) 70, 275
BseRI GAGGAG 1 cut(s) 104
BseSI GKGCMC 1 cut(s) 110
BseXI GCAGC 1 cut(s) 401
BshFI GGCC 1 cut(s) 108
BsiSI CCGG 1 cut(s) 81
BslFI GGGAC 1 cut(s) 323
BslI CCNNNNNNNGG 1 cut(s) 431
BsmFI GGGAC 1 cut(s) 323
BsnI GGCC 1 cut(s) 108
Bsp120I GGGCCC 1 cut(s) 106
Bsp1286I GDGCHC 1 cut(s) 110
Bsp143I GATC 4 cut(s) 3, 60, 122, 145
Bsp19I CCATGG 1 cut(s) 190
BspACI CCGC 1 cut(s) 369
BspANI GGCC 1 cut(s) 108
BspLI GGNNCC 2 cut(s) 108, 311
BspPI GGATC 1 cut(s) 117
BspTI CTTAAG 1 cut(s) 20
BsrI ACTGG 2 cut(s) 70, 275
BssECI CCNNGG 2 cut(s) 103, 190
BssMI GATC 4 cut(s) 3, 60, 122, 145
BssT1I CCWWGG 1 cut(s) 190
Bst2UI CCWGG 1 cut(s) 104
BstAFI CTTAAG 1 cut(s) 20
BstBAI YACGTR 1 cut(s) 303
BstC8I GCNNGC 1 cut(s) 153
BstDSI CCRYGG 1 cut(s) 190
BstF5I GGATG 2 cut(s) 183, 448
BstH2I RGCGCY 1 cut(s) 70
BstHHI GCGC 1 cut(s) 69
BstKTI GATC 4 cut(s) 6, 63, 125, 148
BstMBI GATC 4 cut(s) 3, 60, 122, 145
BstNI CCWGG 1 cut(s) 104
BstNSI RCATGY 1 cut(s) 199
BstPAI GACNNNNGTC 1 cut(s) 134
BstSCI CCNGG 1 cut(s) 102
BstSLI GKGCMC 1 cut(s) 110
BstSNI TACGTA 1 cut(s) 303
BstV1I GCAGC 1 cut(s) 401
BstXI CCANNNNNNTGG 1 cut(s) 233
BsuRI GGCC 1 cut(s) 108
BtgI CCRYGG 1 cut(s) 190
BtsCI GGATG 2 cut(s) 183, 448
BtsIMutI CAGTG 1 cut(s) 282
Cac8I GCNNGC 1 cut(s) 153
CfoI GCGC 1 cut(s) 69
Cfr13I GGNCC 3 cut(s) 106, 107, 310
Csp6I GTAC 3 cut(s) 258, 326, 446
CviAII CATG 3 cut(s) 191, 196, 383
CviJI RGCY 5 cut(s) 51, 108, 151, 376, 458
CviKI_1 RGCY 5 cut(s) 51, 108, 151, 376, 458
CviQI GTAC 3 cut(s) 258, 326, 446
DpnI GATC 4 cut(s) 5, 62, 124, 147
DpnII GATC 4 cut(s) 3, 60, 122, 145
Eco105I TACGTA 1 cut(s) 303
Eco130I CCWWGG 1 cut(s) 190
Eco24I GRGCYC 1 cut(s) 110
Eco47I GGWCC 1 cut(s) 310
Eco57I CTGAAG 1 cut(s) 470
EcoO109I RGGNCCY 2 cut(s) 106, 310
EcoRII CCWGG 1 cut(s) 102
EcoT14I CCWWGG 1 cut(s) 190
EcoT38I GRGCYC 1 cut(s) 110
ErhI CCWWGG 1 cut(s) 190
FaeI CATG 3 cut(s) 194, 199, 386
FaiI YATR 7 cut(s) 8, 192, 197, 300, 384, 438, 487
FaqI GGGAC 1 cut(s) 323
FatI CATG 3 cut(s) 190, 195, 382
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 1 cut(s) 390
FokI GGATG 2 cut(s) 170, 455
FriOI GRGCYC 1 cut(s) 110
Fsp4HI GCNGC 1 cut(s) 390
FspBI CTAG 2 cut(s) 41, 401
GlaI GCGC 1 cut(s) 68
GluI GCNGC 1 cut(s) 390
GsuI CTGGAG 1 cut(s) 86
HaeII RGCGCY 1 cut(s) 70
HaeIII GGCC 1 cut(s) 108
HapII CCGG 1 cut(s) 81
HhaI GCGC 1 cut(s) 69
Hin1II CATG 3 cut(s) 194, 199, 386
Hin6I GCGC 1 cut(s) 67
HinP1I GCGC 1 cut(s) 67
HincII GTYRAC 1 cut(s) 201
HindII GTYRAC 1 cut(s) 201
HpaII CCGG 1 cut(s) 81
Hpy166II GTNNAC 3 cut(s) 201, 272, 497
Hpy188I TCNGA 3 cut(s) 37, 231, 503
Hpy188III TCNNGA 2 cut(s) 164, 401
Hpy8I GTNNAC 3 cut(s) 201, 272, 497
HpyAV CCTTC 1 cut(s) 207
HpyCH4IV ACGT 1 cut(s) 302
HpyCH4V TGCA 1 cut(s) 155
HpySE526I ACGT 1 cut(s) 302
Hsp92II CATG 3 cut(s) 194, 199, 386
HspAI GCGC 1 cut(s) 67
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 4 cut(s) 3, 60, 122, 145
LmnI GCTCC 2 cut(s) 19, 105
LpnPI CCDG 8 cut(s) 83, 89, 94, 116, 149, 226, 288, 413
Lsp1109I GCAGC 1 cut(s) 401
MaeI CTAG 2 cut(s) 41, 401
MaeII ACGT 1 cut(s) 302
MaeIII GTNAC 1 cut(s) 409
MalI GATC 4 cut(s) 5, 62, 124, 147
MboI GATC 4 cut(s) 3, 60, 122, 145
MboII GAAGA 1 cut(s) 70
MhlI GDGCHC 1 cut(s) 110
MluCI AATT 2 cut(s) 284, 356
MmeI TCCRAC 1 cut(s) 71
MnlI CCTC 2 cut(s) 125, 197
MseI TTAA 2 cut(s) 21, 396
MslI CAYNNNNRTG 1 cut(s) 231
MspCI CTTAAG 1 cut(s) 20
MspI CCGG 1 cut(s) 81
MspR9I CCNGG 1 cut(s) 104
MvaI CCWGG 1 cut(s) 104
NcoI CCATGG 1 cut(s) 190
NdeII GATC 4 cut(s) 3, 60, 122, 145
NlaIII CATG 3 cut(s) 194, 199, 386
NlaIV GGNNCC 2 cut(s) 108, 311
NmuCI GTSAC 1 cut(s) 409
NspI RCATGY 1 cut(s) 199
PkrI GCNGC 1 cut(s) 391
Ppu21I YACGTR 1 cut(s) 303
PpuMI RGGWCCY 1 cut(s) 310
PshAI GACNNNNGTC 1 cut(s) 134
Psp5II RGGWCCY 1 cut(s) 310
Psp6I CCWGG 1 cut(s) 102
PspGI CCWGG 1 cut(s) 102
PspN4I GGNNCC 2 cut(s) 108, 311
PspOMI GGGCCC 1 cut(s) 106
PspPI GGNCC 3 cut(s) 106, 107, 310
PspPPI RGGWCCY 1 cut(s) 310
RsaI GTAC 3 cut(s) 259, 327, 447
RsaNI GTAC 3 cut(s) 258, 326, 446
RseI CAYNNNNRTG 1 cut(s) 231
SaqAI TTAA 2 cut(s) 21, 396
SatI GCNGC 1 cut(s) 390
Sau3AI GATC 4 cut(s) 3, 60, 122, 145
Sau96I GGNCC 3 cut(s) 106, 107, 310
ScrFI CCNGG 1 cut(s) 104
SduI GDGCHC 1 cut(s) 110
SetI ASST 6 cut(s) 42, 53, 245, 305, 315, 460
SinI GGWCC 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 231
SmlI CTYRAG 2 cut(s) 20, 116
SmoI CTYRAG 2 cut(s) 20, 116
SnaBI TACGTA 1 cut(s) 303
Sse9I AATT 2 cut(s) 284, 356
SsiI CCGC 1 cut(s) 369
SspMI CTAG 2 cut(s) 41, 401
StyD4I CCNGG 1 cut(s) 102
StyI CCWWGG 1 cut(s) 190
TaiI ACGT 1 cut(s) 305
TaqI TCGA 1 cut(s) 138
TasI AATT 2 cut(s) 284, 356
TatI WGTACW 2 cut(s) 325, 445
Tru1I TTAA 2 cut(s) 21, 396
Tru9I TTAA 2 cut(s) 21, 396
TscAI CASTG 1 cut(s) 282
TseFI GTSAC 1 cut(s) 409
TseI GCWGC 1 cut(s) 389
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 2 cut(s) 172, 399
TspGWI ACGGA 1 cut(s) 77
TspRI CASTG 1 cut(s) 282
Vha464I CTTAAG 1 cut(s) 20
VpaK11BI GGWCC 1 cut(s) 310
XapI RAATTY 1 cut(s) 356
XbaI TCTAGA 1 cut(s) 400
XceI RCATGY 1 cut(s) 199
XspI CTAG 2 cut(s) 41, 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.