Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0002440

Physical Location & Seq

Genomic Coordinates Reverse (-)

18144207 .. 18150827

Gene Structure 6621 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00232500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 903 bp

ATGGCTTGCCATGTCAATTTGGTTCCCCAATCCATGGCCACAGCGTCAGCTAAACCCAAAGCTCCTCCGCCTCCCGCCGCGTGTTCCATTTCAAAGCCATTCATCTCCGCAAAGCTTGTTCACTCGTCGCAATCCTCTTTCGTTTCCACCTTCTCTCCCTCTCGCCCTCAAACCCTAACTCCTCTCCGTTCCAGCAGATCTATGACTGACCTGGCGGTGCGGTGTGCTTCTAAGGCTGAGCCGCCCAGAATTATGATATCCGGGCCGCCCGCTTCGGGTAAAGGAACGCAATGCGAGCTCATTACTCAGAAATATGATTTGGTACATGTCGCTGCTGGAGATTTACTTAGAGCGGAAATTGCATCTGGGAATGAAAATGGGAAGCGTGCAAGGGCCTACATGGACAAAGGACAATTGGTGCCAGATGAAATAGTTGTCATGATGGTGAAAGAGCGTCTATTACAGCCAGATTCTCGAGAAAAAGGCTGGCTCTTGGATGGATACCCTAGGAGCTCATCACAAGCAATTGCTCTTAAGGAATTCGGCTTCGAGCCGGATCTTTTCATTCTTCTTGACGTTCCTGAAGAGATTCTTGTTGACAGAGTAGTTGGAAGAAGGTTAGATCCTGTTACTGGAAAGATCTACCATCTGAAGTATTCTCCTCCAGAGACTCCAGAGATCGCGGCAAGGCTCACCCAGCGTTTTGATGATACAGAAGAAAAGGTGAAACTTCGATTGCACACTCATCATCAAAATGTGGAGGCAGTACTTTCATTATATGAAGACATAACCCTTAAGGTGGACGGGAGTGTTACCAAGGATGATGTGTTTGCCCAAATTGATGGTGCACTGGCAAAGCTTCTTGAGAAAAAGCGGGCTACCACAGAATCTTTGGCAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004017 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009123 GO:0009165 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016776 GO:0018130 GO:0019205 GO:0019438 GO:0019637 GO:0032501 GO:0034641 GO:0034654 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046940 GO:0048871 GO:0050145 GO:0055086 GO:0065007 GO:0065008 GO:0071704 GO:0090407 GO:0097009 GO:1901293 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00230 ko00730 ko01100 ko01110 ko01130 map00230 map00730 map01100 map01110 map01130

Pfam Domains

Protein Families

ADK

Protein Analysis

300

Amino Acids

32.88

Weight (kDa)

8.26

Isoelectric Point (pI)

54.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ADK	PF00406	86 - 262	1.8e-48	Adenylate kinase
AAA_17	PF13207	88 - 206	1.9e-26	AAA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002440)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G47840 AT5G47840
fragaria_vesca	FvH4_5g31400 FvH4_5g31400 FvH4_5g31400 FvH4_5g31400
malus_domestica	MD08G1217400.v1.1 MD15G1404700.v1.1
prunus_persica	Prupe.1G556600_v2.0.a1 Prupe.1G556600_v2.0.a1 Prupe.1G556600_v2.0.a1
pyrus_communis	pycom15g36210
rosa_chinensis	RchiOBHm_Chr1g0329011 RchiOBHm_Chr7g0223711 RchiOBHm_Chr7g0226671
rosa_laevigata	RLG00000001708 RLG00000009936 RLG00000032182 RLG00000032264 RLG00000034316 RLG00000035641 RLG00000036166
rosa_multiflora	Rmu_sc0001903.1_g000022 Rmu_sc0009538.1_g000003
rosa_roxburghii	Rroxscaffold_3G00232500
rosa_rugosa	Rorug07G0218900 Rorug07G0239100
rosa_samantha	Rh2DG023700 Rh4AG027300 Rh7AG361700 Rh7AG387100 Rh7BG353200 Rh7BG373300 Rh7CG380300 Rh7CG406300 Rh7DG357100 Rh7DG385400
rosa_wichuraiana	Rw2G034670 Rw5G032960 Rw7G030750 Rw7G032460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	418
AccB7I	CCANNNNNTGG	1 cut(s)	34
AccBSI	CCGCTC	1 cut(s)	353
AccII	CGCG	2 cut(s)	80, 683
AclWI	GGATC	2 cut(s)	564, 617
AcoI	YGGCCR	1 cut(s)	36
AcsI	RAATTY	1 cut(s)	539
AcuI	CTGAAG	2 cut(s)	603, 671
AfaI	GTAC	2 cut(s)	324, 768
AfiI	CCNNNNNNNGG	4 cut(s)	34, 275, 632, 799
AflII	CTTAAG	2 cut(s)	533, 794
AflIII	ACRYGT	1 cut(s)	325
AgsI	TTSAA	1 cut(s)	93
AjnI	CCWGG	1 cut(s)	210
AjuI	GAANNNNNNNTTGG	2 cut(s)	302, 334
AluBI	AGCT	7 cut(s)	50, 62, 115, 298, 513, 859, 899
AluI	AGCT	7 cut(s)	50, 62, 115, 298, 513, 859, 899
Alw21I	GWGCWC	3 cut(s)	300, 515, 850
Alw26I	GTCTC	1 cut(s)	662
Alw44I	GTGCAC	1 cut(s)	846
AlwI	GGATC	2 cut(s)	564, 617
Ama87I	CYCGRG	1 cut(s)	474
AoxI	GGCC	3 cut(s)	36, 263, 393
ApaLI	GTGCAC	1 cut(s)	846
ApeKI	GCWGC	2 cut(s)	332, 896
ApoI	RAATTY	1 cut(s)	539
Asp700I	GAANNNNTTC	1 cut(s)	588
AspA2I	CCTAGG	1 cut(s)	506
AspS9I	GGNCC	2 cut(s)	263, 393
AsuC2I	CCSGG	1 cut(s)	262
AsuHPI	GGTGA	3 cut(s)	457, 685, 736
AvaI	CYCGRG	1 cut(s)	474
AvrII	CCTAGG	1 cut(s)	506
BaeGI	GKGCMC	1 cut(s)	850
BalI	TGGCCA	1 cut(s)	38
BanI	GGYRCC	1 cut(s)	418
BanII	GRGCYC	2 cut(s)	300, 515
BbsI	GAAGAC	1 cut(s)	789
Bbv12I	GWGCWC	3 cut(s)	300, 515, 850
BbvI	GCAGC	1 cut(s)	319
BccI	CCATC	4 cut(s)	436, 491, 654, 836
BciT130I	CCWGG	1 cut(s)	212
BciVI	GTATCC	1 cut(s)	494
BcnI	CCSGG	1 cut(s)	262
BcoDI	GTCTC	1 cut(s)	662
BfaI	CTAG	1 cut(s)	507
BfrI	CTTAAG	2 cut(s)	533, 794
BfuI	GTATCC	1 cut(s)	494
BglII	AGATCT	2 cut(s)	197, 639
BisI	GCNGC	6 cut(s)	78, 242, 266, 333, 684, 897
BlnI	CCTAGG	1 cut(s)	506
BlpI	GCTNAGC	1 cut(s)	237
BlsI	GCNGC	6 cut(s)	79, 243, 267, 334, 685, 898
BmcAI	AGTACT	1 cut(s)	768
Bme1390I	CCNGG	2 cut(s)	212, 262
BmeT110I	CYCGRG	1 cut(s)	474
BmgT120I	GGNCC	2 cut(s)	263, 393
BmiI	GGNNCC	2 cut(s)	24, 420
BmrFI	CCNGG	2 cut(s)	212, 262
BmsI	GCATC	1 cut(s)	371
BpiI	GAAGAC	1 cut(s)	789
BpmI	CTGGAG	3 cut(s)	357, 648, 657
Bpu1102I	GCTNAGC	1 cut(s)	237
BpuEI	CTTGAG	1 cut(s)	884
BpuMI	CCSGG	1 cut(s)	262
BsaJI	CCNNGG	3 cut(s)	33, 506, 816
BsaXI	ACNNNNNCTCC	4 cut(s)	139, 163, 169, 193
Bsc4I	CCNNNNNNNGG	4 cut(s)	34, 275, 632, 799
Bse1I	ACTGG	2 cut(s)	637, 855
Bse3DI	GCAATG	1 cut(s)	296
BseBI	CCWGG	1 cut(s)	212
BseDI	CCNNGG	3 cut(s)	33, 506, 816
BseGI	GGATG	2 cut(s)	502, 826
BseLI	CCNNNNNNNGG	4 cut(s)	34, 275, 632, 799
BseMI	GCAATG	1 cut(s)	296
BseMII	CTCAG	2 cut(s)	228, 320
BseNI	ACTGG	2 cut(s)	637, 855
BseRI	GAGGAG	3 cut(s)	54, 171, 651
BseSI	GKGCMC	1 cut(s)	850
BseXI	GCAGC	1 cut(s)	319
BseYI	CCCAGC	1 cut(s)	696
Bsh1236I	CGCG	2 cut(s)	80, 683
BshFI	GGCC	3 cut(s)	38, 265, 395
BshNI	GGYRCC	1 cut(s)	418
BsiHKAI	GWGCWC	3 cut(s)	300, 515, 850
BsiHKCI	CYCGRG	1 cut(s)	474
BsiSI	CCGG	2 cut(s)	261, 554
BslI	CCNNNNNNNGG	4 cut(s)	34, 275, 632, 799
BsmAI	GTCTC	1 cut(s)	662
BsnI	GGCC	3 cut(s)	38, 265, 395
BsoBI	CYCGRG	1 cut(s)	474
Bsp1286I	GDGCHC	3 cut(s)	300, 515, 850
Bsp143I	GATC	5 cut(s)	197, 556, 622, 639, 678
Bsp1720I	GCTNAGC	1 cut(s)	237
Bsp19I	CCATGG	1 cut(s)	33
BspANI	GGCC	3 cut(s)	38, 265, 395
BspCNI	CTCAG	2 cut(s)	229, 319
BspFNI	CGCG	2 cut(s)	80, 683
BspHI	TCATGA	1 cut(s)	438
BspLI	GGNNCC	2 cut(s)	24, 420
BspPI	GGATC	2 cut(s)	564, 617
BspT107I	GGYRCC	1 cut(s)	418
BspTI	CTTAAG	2 cut(s)	533, 794
BsrBI	CCGCTC	1 cut(s)	353
BsrDI	GCAATG	1 cut(s)	296
BsrI	ACTGG	2 cut(s)	637, 855
BssECI	CCNNGG	3 cut(s)	33, 506, 816
BssMI	GATC	5 cut(s)	197, 556, 622, 639, 678
BssT1I	CCWWGG	3 cut(s)	33, 506, 816
Bst2UI	CCWGG	1 cut(s)	212
Bst6I	CTCTTC	1 cut(s)	579
BstAFI	CTTAAG	2 cut(s)	533, 794
BstC8I	GCNNGC	6 cut(s)	7, 270, 296, 387, 488, 876
BstDEI	CTNAG	5 cut(s)	231, 237, 306, 347, 900
BstDSI	CCRYGG	1 cut(s)	33
BstF5I	GGATG	2 cut(s)	502, 826
BstFNI	CGCG	2 cut(s)	80, 683
BstKTI	GATC	5 cut(s)	200, 559, 625, 642, 681
BstMAI	GTCTC	1 cut(s)	662
BstMBI	GATC	5 cut(s)	197, 556, 622, 639, 678
BstMWI	GCNNNNNNNGC	4 cut(s)	233, 295, 359, 697
BstNI	CCWGG	1 cut(s)	212
BstNSI	RCATGY	1 cut(s)	329
BstSCI	CCNGG	2 cut(s)	210, 260
BstSLI	GKGCMC	1 cut(s)	850
BstUI	CGCG	2 cut(s)	80, 683
BstV1I	GCAGC	1 cut(s)	319
BstV2I	GAAGAC	1 cut(s)	789
BstX2I	RGATCY	4 cut(s)	197, 556, 622, 639
BstXI	CCANNNNNNTGG	1 cut(s)	842
BstYI	RGATCY	4 cut(s)	197, 556, 622, 639
BsuI	GTATCC	1 cut(s)	494
BsuRI	GGCC	3 cut(s)	38, 265, 395
BtgI	CCRYGG	1 cut(s)	33
BtsCI	GGATG	2 cut(s)	502, 826
BtsIMutI	CAGTG	1 cut(s)	848
Cac8I	GCNNGC	6 cut(s)	7, 270, 296, 387, 488, 876
CciI	TCATGA	1 cut(s)	438
Cfr13I	GGNCC	2 cut(s)	263, 393
CseI	GACGC	2 cut(s)	33, 443
Csp6I	GTAC	2 cut(s)	323, 767
CviAII	CATG	5 cut(s)	11, 34, 326, 400, 439
CviQI	GTAC	2 cut(s)	323, 767
DdeI	CTNAG	5 cut(s)	231, 237, 306, 347, 900
DpnI	GATC	5 cut(s)	199, 558, 624, 641, 680
DpnII	GATC	5 cut(s)	197, 556, 622, 639, 678
EaeI	YGGCCR	1 cut(s)	36
Eam1104I	CTCTTC	1 cut(s)	579
EarI	CTCTTC	1 cut(s)	579
EciI	GGCGGA	1 cut(s)	57
Ecl136II	GAGCTC	2 cut(s)	298, 513
Eco130I	CCWWGG	3 cut(s)	33, 506, 816
Eco24I	GRGCYC	2 cut(s)	300, 515
Eco32I	GATATC	1 cut(s)	258
Eco53kI	GAGCTC	2 cut(s)	298, 513
Eco57I	CTGAAG	2 cut(s)	603, 671
Eco88I	CYCGRG	1 cut(s)	474
EcoICRI	GAGCTC	2 cut(s)	298, 513
EcoO109I	RGGNCCY	1 cut(s)	393
EcoRI	GAATTC	1 cut(s)	539
EcoRII	CCWGG	1 cut(s)	210
EcoRV	GATATC	1 cut(s)	258
EcoT14I	CCWWGG	3 cut(s)	33, 506, 816
EcoT38I	GRGCYC	2 cut(s)	300, 515
ErhI	CCWWGG	3 cut(s)	33, 506, 816
FaeI	CATG	5 cut(s)	14, 37, 329, 403, 442
FalI	AAGNNNNNCTT	2 cut(s)	576, 608
FatI	CATG	5 cut(s)	10, 33, 325, 399, 438
FauI	CCCGC	3 cut(s)	82, 277, 867
Fnu4HI	GCNGC	6 cut(s)	78, 242, 266, 333, 684, 897
FokI	GGATG	2 cut(s)	509, 833
FriOI	GRGCYC	2 cut(s)	300, 515
Fsp4HI	GCNGC	6 cut(s)	78, 242, 266, 333, 684, 897
FspBI	CTAG	1 cut(s)	507
GluI	GCNGC	6 cut(s)	78, 242, 266, 333, 684, 897
GsaI	CCCAGC	1 cut(s)	700
GsuI	CTGGAG	3 cut(s)	357, 648, 657
HaeIII	GGCC	3 cut(s)	38, 265, 395
HapII	CCGG	2 cut(s)	261, 554
HgaI	GACGC	2 cut(s)	33, 443
Hin1II	CATG	5 cut(s)	14, 37, 329, 403, 442
HincII	GTYRAC	1 cut(s)	598
HindII	GTYRAC	1 cut(s)	598
HindIII	AAGCTT	2 cut(s)	113, 857
HinfI	GANTC	4 cut(s)	470, 589, 670, 887
HpaII	CCGG	2 cut(s)	261, 554
HphI	GGTGA	3 cut(s)	457, 685, 736
Hpy166II	GTNNAC	4 cut(s)	121, 598, 802, 848
Hpy188I	TCNGA	2 cut(s)	309, 651
Hpy188III	TCNNGA	8 cut(s)	439, 474, 476, 572, 581, 665, 674, 863
Hpy8I	GTNNAC	4 cut(s)	121, 598, 802, 848
Hpy99I	CGWCG	1 cut(s)	130
HpyAV	CCTTC	2 cut(s)	160, 609
HpyCH4IV	ACGT	1 cut(s)	576
HpyCH4V	TGCA	4 cut(s)	362, 389, 739, 848
HpyF10VI	GCNNNNNNNGC	4 cut(s)	233, 295, 359, 697
HpyF3I	CTNAG	5 cut(s)	231, 237, 306, 347, 900
HpySE526I	ACGT	1 cut(s)	576
Hsp92II	CATG	5 cut(s)	14, 37, 329, 403, 442
Kzo9I	GATC	5 cut(s)	197, 556, 622, 639, 678
LmnI	GCTCC	2 cut(s)	67, 510
Lsp1109I	GCAGC	1 cut(s)	319
LweI	GCATC	1 cut(s)	371
MaeI	CTAG	1 cut(s)	507
MaeII	ACGT	1 cut(s)	576
MaeIII	GTNAC	2 cut(s)	628, 811
MalI	GATC	5 cut(s)	199, 558, 624, 641, 680
MbiI	CCGCTC	1 cut(s)	353
MboI	GATC	5 cut(s)	197, 556, 622, 639, 678
MboII	GAAGA	5 cut(s)	560, 596, 624, 728, 794
MfeI	CAATTG	2 cut(s)	413, 525
MflI	RGATCY	4 cut(s)	197, 556, 622, 639
MhlI	GDGCHC	3 cut(s)	300, 515, 850
MlsI	TGGCCA	1 cut(s)	38
MluCI	AATT	7 cut(s)	16, 249, 357, 413, 525, 539, 837
MluNI	TGGCCA	1 cut(s)	38
MlyI	GAGTC	1 cut(s)	664
MmeI	TCCRAC	1 cut(s)	589
MnlI	CCTC	8 cut(s)	75, 81, 145, 169, 177, 192, 672, 754
Mox20I	TGGCCA	1 cut(s)	38
MroXI	GAANNNNTTC	1 cut(s)	588
MscI	TGGCCA	1 cut(s)	38
MseI	TTAA	2 cut(s)	534, 795
MslI	CAYNNNNRTG	2 cut(s)	443, 753
Msp20I	TGGCCA	1 cut(s)	38
MspCI	CTTAAG	2 cut(s)	533, 794
MspI	CCGG	2 cut(s)	261, 554
MspR9I	CCNGG	2 cut(s)	212, 262
MunI	CAATTG	2 cut(s)	413, 525
MvaI	CCWGG	1 cut(s)	212
MvnI	CGCG	2 cut(s)	80, 683
MwoI	GCNNNNNNNGC	4 cut(s)	233, 295, 359, 697
NciI	CCSGG	1 cut(s)	262
NcoI	CCATGG	1 cut(s)	33
NdeII	GATC	5 cut(s)	197, 556, 622, 639, 678
NlaIII	CATG	5 cut(s)	14, 37, 329, 403, 442
NlaIV	GGNNCC	2 cut(s)	24, 420
NspI	RCATGY	1 cut(s)	329
PaeR7I	CTCGAG	1 cut(s)	474
PagI	TCATGA	1 cut(s)	438
PciI	ACATGT	1 cut(s)	325
PdmI	GAANNNNTTC	1 cut(s)	588
PfeI	GAWTC	3 cut(s)	470, 589, 887
PflMI	CCANNNNNTGG	1 cut(s)	34
PkrI	GCNGC	6 cut(s)	79, 243, 267, 334, 685, 898
PleI	GAGTC	1 cut(s)	664
PpsI	GAGTC	1 cut(s)	664
PscI	ACATGT	1 cut(s)	325
Psp124BI	GAGCTC	2 cut(s)	300, 515
Psp6I	CCWGG	1 cut(s)	210
PspFI	CCCAGC	1 cut(s)	696
PspGI	CCWGG	1 cut(s)	210
PspN4I	GGNNCC	2 cut(s)	24, 420
PspPI	GGNCC	2 cut(s)	263, 393
PsuI	RGATCY	4 cut(s)	197, 556, 622, 639
RsaI	GTAC	2 cut(s)	324, 768
RsaNI	GTAC	2 cut(s)	323, 767
RseI	CAYNNNNRTG	2 cut(s)	443, 753
SacI	GAGCTC	2 cut(s)	300, 515
SaqAI	TTAA	2 cut(s)	534, 795
SatI	GCNGC	6 cut(s)	78, 242, 266, 333, 684, 897
Sau3AI	GATC	5 cut(s)	197, 556, 622, 639, 678
Sau96I	GGNCC	2 cut(s)	263, 393
ScaI	AGTACT	1 cut(s)	768
SchI	GAGTC	1 cut(s)	664
ScrFI	CCNGG	2 cut(s)	212, 262
SduI	GDGCHC	3 cut(s)	300, 515, 850
SfaNI	GCATC	1 cut(s)	371
Sfr274I	CTCGAG	1 cut(s)	474
SlaI	CTCGAG	1 cut(s)	474
SmiMI	CAYNNNNRTG	2 cut(s)	443, 753
SmlI	CTYRAG	4 cut(s)	474, 533, 794, 863
SmoI	CTYRAG	4 cut(s)	474, 533, 794, 863
Sse9I	AATT	7 cut(s)	16, 249, 357, 413, 525, 539, 837
SspMI	CTAG	1 cut(s)	507
SstI	GAGCTC	2 cut(s)	300, 515
StyD4I	CCNGG	2 cut(s)	210, 260
StyI	CCWWGG	3 cut(s)	33, 506, 816
TaiI	ACGT	1 cut(s)	579
TaqI	TCGA	3 cut(s)	475, 549, 733
TasI	AATT	7 cut(s)	16, 249, 357, 413, 525, 539, 837
TatI	WGTACW	1 cut(s)	766
TauI	GCSGC	4 cut(s)	80, 244, 268, 686
TfiI	GAWTC	3 cut(s)	470, 589, 887
Tru1I	TTAA	2 cut(s)	534, 795
Tru9I	TTAA	2 cut(s)	534, 795
TscAI	CASTG	1 cut(s)	855
TseI	GCWGC	2 cut(s)	332, 896
TspDTI	ATGAA	6 cut(s)	91, 387, 441, 553, 762, 795
TspGWI	ACGGA	1 cut(s)	176
TspRI	CASTG	1 cut(s)	855
Van91I	CCANNNNNTGG	1 cut(s)	34
Vha464I	CTTAAG	2 cut(s)	533, 794
VneI	GTGCAC	1 cut(s)	846
XapI	RAATTY	1 cut(s)	539
XceI	RCATGY	1 cut(s)	329
XcmI	CCANNNNNNNNNTGG	1 cut(s)	889
XhoI	CTCGAG	1 cut(s)	474
XmaJI	CCTAGG	1 cut(s)	506
XmnI	GAANNNNTTC	1 cut(s)	588
XspI	CTAG	1 cut(s)	507
ZrmI	AGTACT	1 cut(s)	768

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.