Rroxscaffold_4G00298010

rRNA-processing protein FCF1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002196
Physical Location & Seq
Forward (+)
17888786 .. 17892846
4061 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00298010.1

Sequence Viewer

Length: 465 bp
ATGTTTCTTCGGCGCTGTTCTTTAAATACAACACTGCTTTGGGACCGCCTTACGGGTTCGGTGGATACCAACTTTATCAATTTCTCTATCCGAATAAGCACTCCTTGTATCATAGATTGTGTAATGACAGAGCTTGAGAAGTTGGGTCAGAAATATCGAGTGGCTCTGAGGATTGCCAAAGATCCCCGTTTCGAGAGACTACTGTGTACTCATAAAGGAACCTATGCTGATGACTGTCTTGTTGATAGAGTCACCCAGCATAAATGCTACATTGTCGCTACATGTGATAGAGATTTAAAGCGAAGGATCCGAAAGGTCCCTGGTGTGCCGATCATGTATATCACTCAACACAAGTACTCAATTGAACGATTGCCTGAAGCAACAATTGGTGGAGGTACTTTGAAGTTATGGGGTGATGACATTGATGTTATTGCCGAGCTCCAAGGTATTGATGATGGGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
Fcf1

Protein Analysis

154

Amino Acids

17.65

Weight (kDa)

8.48

Isoelectric Point (pI)

30.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fcf1 PF04900 34 - 123 3.3e-32 Fcf1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 46
AclWI GGATC 3 cut(s) 176, 301, 314
AcuI CTGAAG 1 cut(s) 396
AfaI GTAC 3 cut(s) 208, 356, 397
AfiI CCNNNNNNNGG 1 cut(s) 52
AflIII ACRYGT 1 cut(s) 281
AgsI TTSAA 2 cut(s) 365, 403
AjnI CCWGG 1 cut(s) 319
AjuI GAANNNNNNNTTGG 2 cut(s) 369, 401
AluBI AGCT 2 cut(s) 133, 439
AluI AGCT 2 cut(s) 133, 439
Alw21I GWGCWC 1 cut(s) 441
Alw26I GTCTC 1 cut(s) 190
AlwI GGATC 3 cut(s) 176, 301, 314
AspLEI GCGC 1 cut(s) 15
AspS9I GGNCC 2 cut(s) 43, 316
AsuHPI GGTGA 2 cut(s) 244, 425
AvaII GGWCC 2 cut(s) 43, 316
BamHI GGATCC 1 cut(s) 306
BanII GRGCYC 1 cut(s) 441
Bbv12I GWGCWC 1 cut(s) 441
BccI CCATC 1 cut(s) 449
BciT130I CCWGG 1 cut(s) 321
BciVI GTATCC 1 cut(s) 58
BcoDI GTCTC 1 cut(s) 190
BfoI RGCGCY 1 cut(s) 16
BfuI GTATCC 1 cut(s) 58
BmcAI AGTACT 1 cut(s) 356
Bme1390I CCNGG 1 cut(s) 321
Bme18I GGWCC 2 cut(s) 43, 316
BmgT120I GGNCC 2 cut(s) 43, 316
BmiI GGNNCC 4 cut(s) 44, 220, 308, 318
BmrFI CCNGG 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 155
BsaJI CCNNGG 2 cut(s) 319, 442
Bsc4I CCNNNNNNNGG 1 cut(s) 52
BseBI CCWGG 1 cut(s) 321
BseDI CCNNGG 2 cut(s) 319, 442
BseLI CCNNNNNNNGG 1 cut(s) 52
BseMII CTCAG 1 cut(s) 158
BseYI CCCAGC 1 cut(s) 255
BsiHKAI GWGCWC 1 cut(s) 441
BslFI GGGAC 2 cut(s) 56, 302
BslI CCNNNNNNNGG 1 cut(s) 52
BsmAI GTCTC 1 cut(s) 190
BsmFI GGGAC 2 cut(s) 56, 302
Bsp1286I GDGCHC 1 cut(s) 441
Bsp143I GATC 3 cut(s) 181, 306, 330
BspACI CCGC 1 cut(s) 46
BspCNI CTCAG 1 cut(s) 159
BspLI GGNNCC 4 cut(s) 44, 220, 308, 318
BspPI GGATC 3 cut(s) 176, 301, 314
BssECI CCNNGG 2 cut(s) 319, 442
BssMI GATC 3 cut(s) 181, 306, 330
BssT1I CCWWGG 1 cut(s) 442
Bst2UI CCWGG 1 cut(s) 321
Bst4CI ACNGT 2 cut(s) 204, 236
BstDEI CTNAG 1 cut(s) 167
BstH2I RGCGCY 1 cut(s) 16
BstHHI GCGC 1 cut(s) 15
BstKTI GATC 3 cut(s) 184, 309, 333
BstMAI GTCTC 1 cut(s) 190
BstMBI GATC 3 cut(s) 181, 306, 330
BstNI CCWGG 1 cut(s) 321
BstNSI RCATGY 1 cut(s) 285
BstSCI CCNGG 1 cut(s) 319
BstX2I RGATCY 2 cut(s) 181, 306
BstYI RGATCY 2 cut(s) 181, 306
BsuI GTATCC 1 cut(s) 58
BtsI GCAGTG 1 cut(s) 32
BtsIMutI CAGTG 1 cut(s) 32
CfoI GCGC 1 cut(s) 15
Cfr13I GGNCC 2 cut(s) 43, 316
Csp6I GTAC 3 cut(s) 207, 355, 396
CviAII CATG 2 cut(s) 282, 334
CviJI RGCY 3 cut(s) 133, 164, 439
CviKI_1 RGCY 3 cut(s) 133, 164, 439
CviQI GTAC 3 cut(s) 207, 355, 396
DdeI CTNAG 1 cut(s) 167
DpnI GATC 3 cut(s) 183, 308, 332
DpnII GATC 3 cut(s) 181, 306, 330
DraI TTTAAA 2 cut(s) 24, 297
Ecl136II GAGCTC 1 cut(s) 439
Eco130I CCWWGG 1 cut(s) 442
Eco24I GRGCYC 1 cut(s) 441
Eco47I GGWCC 2 cut(s) 43, 316
Eco53kI GAGCTC 1 cut(s) 439
Eco57I CTGAAG 1 cut(s) 396
EcoICRI GAGCTC 1 cut(s) 439
EcoO109I RGGNCCY 1 cut(s) 316
EcoRII CCWGG 1 cut(s) 319
EcoT14I CCWWGG 1 cut(s) 442
EcoT38I GRGCYC 1 cut(s) 441
ErhI CCWWGG 1 cut(s) 442
FaeI CATG 2 cut(s) 285, 337
FaiI YATR 9 cut(s) 113, 213, 225, 261, 283, 335, 339, 409, 463
FalI AAGNNNNNCTT 2 cut(s) 88, 120
FaqI GGGAC 2 cut(s) 56, 302
FatI CATG 2 cut(s) 281, 333
FriOI GRGCYC 1 cut(s) 441
GlaI GCGC 1 cut(s) 14
GsaI CCCAGC 1 cut(s) 259
HaeII RGCGCY 1 cut(s) 16
HhaI GCGC 1 cut(s) 15
Hin1II CATG 2 cut(s) 285, 337
Hin6I GCGC 1 cut(s) 13
HinP1I GCGC 1 cut(s) 13
HinfI GANTC 1 cut(s) 249
HphI GGTGA 2 cut(s) 244, 425
Hpy166II GTNNAC 1 cut(s) 207
Hpy188I TCNGA 4 cut(s) 92, 150, 168, 311
Hpy188III TCNNGA 1 cut(s) 193
Hpy8I GTNNAC 1 cut(s) 207
HpyAV CCTTC 1 cut(s) 297
HpyCH4III ACNGT 2 cut(s) 204, 236
HpyF3I CTNAG 1 cut(s) 167
Hsp92II CATG 2 cut(s) 285, 337
HspAI GCGC 1 cut(s) 13
Kzo9I GATC 3 cut(s) 181, 306, 330
LmnI GCTCC 1 cut(s) 444
LpnPI CCDG 4 cut(s) 269, 306, 333, 387
MaeIII GTNAC 1 cut(s) 250
MalI GATC 3 cut(s) 183, 308, 332
MboI GATC 3 cut(s) 181, 306, 330
MfeI CAATTG 2 cut(s) 360, 384
MflI RGATCY 2 cut(s) 181, 306
MhlI GDGCHC 1 cut(s) 441
MluCI AATT 3 cut(s) 79, 360, 384
MlyI GAGTC 1 cut(s) 258
MnlI CCTC 2 cut(s) 162, 386
MseI TTAA 2 cut(s) 23, 296
MspR9I CCNGG 1 cut(s) 321
MunI CAATTG 2 cut(s) 360, 384
MvaI CCWGG 1 cut(s) 321
NdeII GATC 3 cut(s) 181, 306, 330
NlaIII CATG 2 cut(s) 285, 337
NlaIV GGNNCC 4 cut(s) 44, 220, 308, 318
NmeAIII GCCGAG 1 cut(s) 460
NmuCI GTSAC 1 cut(s) 250
NspI RCATGY 1 cut(s) 285
PciI ACATGT 1 cut(s) 281
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
PpuMI RGGWCCY 1 cut(s) 316
PscI ACATGT 1 cut(s) 281
Psp124BI GAGCTC 1 cut(s) 441
Psp5II RGGWCCY 1 cut(s) 316
Psp6I CCWGG 1 cut(s) 319
PspFI CCCAGC 1 cut(s) 255
PspGI CCWGG 1 cut(s) 319
PspN4I GGNNCC 4 cut(s) 44, 220, 308, 318
PspPI GGNCC 2 cut(s) 43, 316
PspPPI RGGWCCY 1 cut(s) 316
PsuI RGATCY 2 cut(s) 181, 306
RsaI GTAC 3 cut(s) 208, 356, 397
RsaNI GTAC 3 cut(s) 207, 355, 396
SacI GAGCTC 1 cut(s) 441
SaqAI TTAA 2 cut(s) 23, 296
Sau3AI GATC 3 cut(s) 181, 306, 330
Sau96I GGNCC 2 cut(s) 43, 316
ScaI AGTACT 1 cut(s) 356
SchI GAGTC 1 cut(s) 258
ScrFI CCNGG 1 cut(s) 321
SduI GDGCHC 1 cut(s) 441
SetI ASST 6 cut(s) 135, 224, 318, 397, 441, 448
SinI GGWCC 2 cut(s) 43, 316
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 3 cut(s) 79, 360, 384
SsiI CCGC 1 cut(s) 46
SstI GAGCTC 1 cut(s) 441
StyD4I CCNGG 1 cut(s) 319
StyI CCWWGG 1 cut(s) 442
TaaI ACNGT 2 cut(s) 204, 236
TaqI TCGA 2 cut(s) 157, 192
TasI AATT 3 cut(s) 79, 360, 384
TatI WGTACW 2 cut(s) 206, 354
Tru1I TTAA 2 cut(s) 23, 296
Tru9I TTAA 2 cut(s) 23, 296
TscAI CASTG 1 cut(s) 39
TseFI GTSAC 1 cut(s) 250
Tsp45I GTSAC 1 cut(s) 250
TspRI CASTG 1 cut(s) 39
VpaK11BI GGWCC 2 cut(s) 43, 316
XceI RCATGY 1 cut(s) 285
ZrmI AGTACT 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.