Rroxscaffold_4G00331390

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0004876
Physical Location & Seq
Forward (+)
65302815 .. 65308178
5364 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00331390.1

Sequence Viewer

Length: 666 bp
ATGGAAGATTTCGTACACCTCTGCAACCACCTCTTCGGATCTCGGGTTTCTTTGCTAAGATCTATTTTGGTTTGGTTGTTGTTATCCTTTTATGCAAGATCTTTTCGTAATCGGTCAACCGGAGAATGTATGGCGGCTCTGTCCGTATCCTCACCTCTGCAGACCACCCTTGGTTACCAATCCTTGCTCGCGATTTCCATCGGAGCTCAAGTGACGAATCTGCTATGGCGGCTGCAAAGTGGAGAGGAAGAACAAGCTTTGATTAGGGCTTGGGTTTTTGGGCTGCTTGGGCTTTTAATTAGGTTGGGAGGGTCGGACCCTTCTAGCGCCTCCGGCGTAGTACCAAATGGGGATCCCACTATGTCTAGGTATTTGATGGTACAATCGGTAGGTCTATTTACTATGTACCACCGTGGGTACTACCACTATCTTCATGTCCGCCTAGATGACGGCGAAGGATATGTAACGAGTCCTCGCGACGATATATTTGCAATTAAGATACGTGCGTATCCAACAGCTCGCGGCCAAGAAGATTACGTTCGGGACTCCAAAAGGCTAAAGACGATACAACGAAACACAACTCTCAAATCAACTAATCGCACAGATTATCAAAGCCTCTCCGGAGCAAACCTACACTCGCAACCTAGTTGCAAACCAGCGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

221

Amino Acids

24.77

Weight (kDa)

9.45

Isoelectric Point (pI)

57.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 191, 477, 522
AccIII TCCGGA 1 cut(s) 620
AciI CCGC 4 cut(s) 134, 229, 439, 522
AclWI GGATC 3 cut(s) 46, 347, 360
AcoI YGGCCR 1 cut(s) 523
AfaI GTAC 5 cut(s) 15, 342, 381, 407, 419
AluBI AGCT 3 cut(s) 206, 257, 518
AluI AGCT 3 cut(s) 206, 257, 518
Alw21I GWGCWC 1 cut(s) 208
AlwI GGATC 3 cut(s) 46, 347, 360
Ama87I CYCGRG 1 cut(s) 42
Aor13HI TCCGGA 1 cut(s) 620
AoxI GGCC 1 cut(s) 523
ApeKI GCWGC 2 cut(s) 232, 283
ArsI GACNNNNNNTTYG 2 cut(s) 470, 502
AspLEI GCGC 1 cut(s) 329
AspS9I GGNCC 1 cut(s) 316
AsuHPI GGTGA 1 cut(s) 144
AvaI CYCGRG 1 cut(s) 42
AvaII GGWCC 1 cut(s) 316
BaeI ACNNNNGTAYC 4 cut(s) 491, 491, 524, 524
BamHI GGATCC 1 cut(s) 352
BanII GRGCYC 1 cut(s) 208
BarI GAAGNNNNNNTAC 1 cut(s) 29
Bbv12I GWGCWC 1 cut(s) 208
BbvI GCAGC 2 cut(s) 219, 270
BccI CCATC 2 cut(s) 206, 370
BceAI ACGGC 1 cut(s) 466
BcgI CGANNNNNNTGC 2 cut(s) 470, 504
BciVI GTATCC 2 cut(s) 157, 519
BfaI CTAG 4 cut(s) 324, 366, 443, 645
BfmI CTRYAG 1 cut(s) 158
BfoI RGCGCY 1 cut(s) 330
BfuI GTATCC 2 cut(s) 157, 519
BglII AGATCT 2 cut(s) 59, 98
BisI GCNGC 5 cut(s) 135, 230, 233, 284, 523
BlsI GCNGC 5 cut(s) 136, 231, 234, 285, 524
Bme18I GGWCC 1 cut(s) 316
BmeT110I CYCGRG 1 cut(s) 42
BmgT120I GGNCC 1 cut(s) 316
BmiI GGNNCC 2 cut(s) 318, 354
BpuEI CTTGAG 1 cut(s) 192
BsaAI YACGTR 1 cut(s) 503
BsaBI GATNNNNATC 1 cut(s) 197
BsaJI CCNNGG 2 cut(s) 169, 412
BsaWI WCCGGW 2 cut(s) 119, 620
BsaXI ACNNNNNCTCC 2 cut(s) 195, 225
Bse8I GATNNNNATC 1 cut(s) 197
BseAI TCCGGA 1 cut(s) 620
BseDI CCNNGG 2 cut(s) 169, 412
BseJI GATNNNNATC 1 cut(s) 197
BseXI GCAGC 2 cut(s) 219, 270
Bsh1236I CGCG 3 cut(s) 191, 477, 522
BshFI GGCC 1 cut(s) 525
BsiHKAI GWGCWC 1 cut(s) 208
BsiHKCI CYCGRG 1 cut(s) 42
BsiSI CCGG 3 cut(s) 120, 333, 621
BslFI GGGAC 1 cut(s) 557
BsmFI GGGAC 1 cut(s) 557
BsnI GGCC 1 cut(s) 525
BsoBI CYCGRG 1 cut(s) 42
Bsp1286I GDGCHC 1 cut(s) 208
Bsp13I TCCGGA 1 cut(s) 620
Bsp143I GATC 4 cut(s) 38, 59, 98, 352
Bsp68I TCGCGA 2 cut(s) 191, 477
BspACI CCGC 4 cut(s) 134, 229, 439, 522
BspANI GGCC 1 cut(s) 525
BspEI TCCGGA 1 cut(s) 620
BspFNI CGCG 3 cut(s) 191, 477, 522
BspLI GGNNCC 2 cut(s) 318, 354
BspMAI CTGCAG 1 cut(s) 162
BspPI GGATC 3 cut(s) 46, 347, 360
BssECI CCNNGG 2 cut(s) 169, 412
BssMI GATC 4 cut(s) 38, 59, 98, 352
BssT1I CCWWGG 1 cut(s) 169
Bst4CI ACNGT 1 cut(s) 413
Bst6I CTCTTC 1 cut(s) 38
BstBAI YACGTR 1 cut(s) 503
BstC8I GCNNGC 2 cut(s) 189, 520
BstDEI CTNAG 1 cut(s) 56
BstDSI CCRYGG 1 cut(s) 412
BstEII GGTNACC 1 cut(s) 173
BstFNI CGCG 3 cut(s) 191, 477, 522
BstH2I RGCGCY 1 cut(s) 330
BstHHI GCGC 1 cut(s) 329
BstKTI GATC 4 cut(s) 41, 62, 101, 355
BstMBI GATC 4 cut(s) 38, 59, 98, 352
BstMWI GCNNNNNNNGC 3 cut(s) 229, 289, 333
BstPI GGTNACC 1 cut(s) 173
BstSFI CTRYAG 1 cut(s) 158
BstUI CGCG 3 cut(s) 191, 477, 522
BstV1I GCAGC 2 cut(s) 219, 270
BstX2I RGATCY 4 cut(s) 38, 59, 98, 352
BstYI RGATCY 4 cut(s) 38, 59, 98, 352
BsuI GTATCC 2 cut(s) 157, 519
BsuRI GGCC 1 cut(s) 525
BtgI CCRYGG 1 cut(s) 412
BtuMI TCGCGA 2 cut(s) 191, 477
Cac8I GCNNGC 2 cut(s) 189, 520
CfoI GCGC 1 cut(s) 329
Cfr13I GGNCC 1 cut(s) 316
Csp6I GTAC 5 cut(s) 14, 341, 380, 406, 418
CviAII CATG 1 cut(s) 434
CviQI GTAC 5 cut(s) 14, 341, 380, 406, 418
DdeI CTNAG 1 cut(s) 56
DpnI GATC 4 cut(s) 40, 61, 100, 354
DpnII GATC 4 cut(s) 38, 59, 98, 352
EaeI YGGCCR 1 cut(s) 523
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
EciI GGCGGA 1 cut(s) 428
Ecl136II GAGCTC 1 cut(s) 206
Eco130I CCWWGG 1 cut(s) 169
Eco24I GRGCYC 1 cut(s) 208
Eco47I GGWCC 1 cut(s) 316
Eco53kI GAGCTC 1 cut(s) 206
Eco88I CYCGRG 1 cut(s) 42
Eco91I GGTNACC 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 206
EcoO65I GGTNACC 1 cut(s) 173
EcoT14I CCWWGG 1 cut(s) 169
EcoT38I GRGCYC 1 cut(s) 208
ErhI CCWWGG 1 cut(s) 169
FaeI CATG 1 cut(s) 437
FaiI YATR 8 cut(s) 93, 131, 226, 362, 404, 435, 462, 485
FaqI GGGAC 1 cut(s) 557
FatI CATG 1 cut(s) 433
Fnu4HI GCNGC 5 cut(s) 135, 230, 233, 284, 523
FriOI GRGCYC 1 cut(s) 208
Fsp4HI GCNGC 5 cut(s) 135, 230, 233, 284, 523
FspBI CTAG 4 cut(s) 324, 366, 443, 645
GlaI GCGC 1 cut(s) 328
GluI GCNGC 5 cut(s) 135, 230, 233, 284, 523
HaeII RGCGCY 1 cut(s) 330
HaeIII GGCC 1 cut(s) 525
HapII CCGG 3 cut(s) 120, 333, 621
HhaI GCGC 1 cut(s) 329
Hin1II CATG 1 cut(s) 437
Hin6I GCGC 1 cut(s) 327
HinP1I GCGC 1 cut(s) 327
HincII GTYRAC 1 cut(s) 117
HindII GTYRAC 1 cut(s) 117
HindIII AAGCTT 1 cut(s) 255
HinfI GANTC 3 cut(s) 217, 469, 545
HpaII CCGG 3 cut(s) 120, 333, 621
HphI GGTGA 1 cut(s) 144
Hpy166II GTNNAC 2 cut(s) 16, 117
Hpy188I TCNGA 3 cut(s) 38, 203, 316
Hpy188III TCNNGA 4 cut(s) 190, 476, 542, 621
Hpy8I GTNNAC 2 cut(s) 16, 117
Hpy99I CGWCG 1 cut(s) 482
HpyAV CCTTC 2 cut(s) 330, 449
HpyCH4III ACNGT 1 cut(s) 413
HpyCH4IV ACGT 2 cut(s) 502, 537
HpyCH4V TGCA 6 cut(s) 24, 95, 160, 235, 491, 651
HpyF10VI GCNNNNNNNGC 3 cut(s) 229, 289, 333
HpyF3I CTNAG 1 cut(s) 56
HpySE526I ACGT 2 cut(s) 502, 537
Hsp92II CATG 1 cut(s) 437
HspAI GCGC 1 cut(s) 327
Kpn2I TCCGGA 1 cut(s) 620
Kzo9I GATC 4 cut(s) 38, 59, 98, 352
LmnI GCTCC 2 cut(s) 203, 623
LpnPI CCDG 3 cut(s) 133, 346, 634
Lsp1109I GCAGC 2 cut(s) 219, 270
MaeI CTAG 4 cut(s) 324, 366, 443, 645
MaeII ACGT 2 cut(s) 502, 537
MaeIII GTNAC 3 cut(s) 173, 211, 463
MalI GATC 4 cut(s) 40, 61, 100, 354
MboI GATC 4 cut(s) 38, 59, 98, 352
MboII GAAGA 5 cut(s) 17, 25, 260, 422, 542
MflI RGATCY 4 cut(s) 38, 59, 98, 352
MhlI GDGCHC 1 cut(s) 208
MluCI AATT 2 cut(s) 297, 492
MlyI GAGTC 2 cut(s) 478, 539
MmeI TCCRAC 2 cut(s) 294, 536
MnlI CCTC 9 cut(s) 29, 41, 160, 165, 238, 302, 340, 483, 626
MroI TCCGGA 1 cut(s) 620
MseI TTAA 2 cut(s) 296, 495
MspI CCGG 3 cut(s) 120, 333, 621
MvnI CGCG 3 cut(s) 191, 477, 522
MwoI GCNNNNNNNGC 3 cut(s) 229, 289, 333
NdeII GATC 4 cut(s) 38, 59, 98, 352
NlaIII CATG 1 cut(s) 437
NlaIV GGNNCC 2 cut(s) 318, 354
NmuCI GTSAC 1 cut(s) 211
NruI TCGCGA 2 cut(s) 191, 477
PfeI GAWTC 1 cut(s) 217
PkrI GCNGC 5 cut(s) 136, 231, 234, 285, 524
PleI GAGTC 2 cut(s) 477, 539
PpsI GAGTC 2 cut(s) 477, 539
Ppu21I YACGTR 1 cut(s) 503
Psp124BI GAGCTC 1 cut(s) 208
PspEI GGTNACC 1 cut(s) 173
PspN4I GGNNCC 2 cut(s) 318, 354
PspPI GGNCC 1 cut(s) 316
PstI CTGCAG 1 cut(s) 162
PsuI RGATCY 4 cut(s) 38, 59, 98, 352
RruI TCGCGA 2 cut(s) 191, 477
RsaI GTAC 5 cut(s) 15, 342, 381, 407, 419
RsaNI GTAC 5 cut(s) 14, 341, 380, 406, 418
SacI GAGCTC 1 cut(s) 208
SaqAI TTAA 2 cut(s) 296, 495
SatI GCNGC 5 cut(s) 135, 230, 233, 284, 523
Sau3AI GATC 4 cut(s) 38, 59, 98, 352
Sau96I GGNCC 1 cut(s) 316
SchI GAGTC 2 cut(s) 478, 539
SduI GDGCHC 1 cut(s) 208
SfcI CTRYAG 1 cut(s) 158
SinI GGWCC 1 cut(s) 316
SmlI CTYRAG 1 cut(s) 207
SmoI CTYRAG 1 cut(s) 207
Sse9I AATT 2 cut(s) 297, 492
SsiI CCGC 4 cut(s) 134, 229, 439, 522
SspMI CTAG 4 cut(s) 324, 366, 443, 645
SstI GAGCTC 1 cut(s) 208
StyI CCWWGG 1 cut(s) 169
TaaI ACNGT 1 cut(s) 413
TaiI ACGT 2 cut(s) 505, 540
TaqII GACCGA 1 cut(s) 102
TasI AATT 2 cut(s) 297, 492
TauI GCSGC 3 cut(s) 137, 232, 525
TfiI GAWTC 1 cut(s) 217
Tru1I TTAA 2 cut(s) 296, 495
Tru9I TTAA 2 cut(s) 296, 495
TseFI GTSAC 1 cut(s) 211
TseI GCWGC 2 cut(s) 232, 283
Tsp45I GTSAC 1 cut(s) 211
TspDTI ATGAA 1 cut(s) 422
TspGWI ACGGA 1 cut(s) 133
VpaK11BI GGWCC 1 cut(s) 316
XspI CTAG 4 cut(s) 324, 366, 443, 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.