Rh4DG140100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004876
Physical Location & Seq
Reverse (-)
27847895 .. 27851953
4059 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG140100.1

Sequence Viewer

Length: 627 bp
ATGGTCATCATTAACCTTGCGACTCTCTGTTTCTTGCTGCTTGATGTGTTTGTTTGTTCATATGGGAAGCCGCCTTGGCCTGGGGCTTCTCTTTCTCCTTTTGTTGTCAACCAAGTGTTCCGATTAGAGGATGCAGTGGTGGACTTCTTGGTGGCTGGATTATCCAGTGATCGTCCGCTCTGGCTGGGAGCTAGGGCCAGAAAACTGGATCTGAGTTCGGGTCGAAACTTCACGGGGAATTCGATGGTGGATTGTTCTATTGGGTTTCTGTCAAATGCTTTTGTTGGTTTCCGAAGGTGGCAGGTCAAGAATAACGACGCTCAGGGTGGAAGGGCTACCGGTGGTTGTGCAGGTTCGAGACCGGCCTCGCGTCGAGGAACTTCTGCATACACGACGACGTCCGTATCCCTGGTGTCGGGTCTAGGATTGGGATCGTTACTTGAGCACTGGATTGGGATCGGCTTCAGTTGTCAGTTTCAGGTTCGGGAGTGGCTTCTGTCTCTGCCGGCTGGTGTTGGAAGTGTTCCCATAGCAGATGCCAAGCATAGGGCTCTTTGGGCCTTGATTTGGACAACAGGCTTATATTCTCTTTCGGCGTTCACAGAATCACAGGTTTACAGTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

208

Amino Acids

22.38

Weight (kDa)

9.16

Isoelectric Point (pI)

26.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 401
Acc36I ACCTGC 2 cut(s) 292, 341
AccBSI CCGCTC 1 cut(s) 178
AccII CGCG 1 cut(s) 370
AciI CCGC 2 cut(s) 71, 176
AclWI GGATC 3 cut(s) 216, 439, 464
AcsI RAATTY 1 cut(s) 238
AcuI CTGAAG 1 cut(s) 448
AcyI GRCGYC 1 cut(s) 398
AfiI CCNNNNNNNGG 5 cut(s) 80, 127, 415, 546, 567
AgeI ACCGGT 1 cut(s) 338
AjnI CCWGG 2 cut(s) 79, 408
AluBI AGCT 1 cut(s) 191
AluI AGCT 1 cut(s) 191
Alw21I GWGCWC 1 cut(s) 447
Alw26I GTCTC 2 cut(s) 352, 504
AlwI GGATC 3 cut(s) 216, 439, 464
AoxI GGCC 4 cut(s) 77, 195, 363, 558
ApeKI GCWGC 1 cut(s) 37
ApoI RAATTY 1 cut(s) 238
AsiGI ACCGGT 1 cut(s) 338
AspS9I GGNCC 2 cut(s) 195, 558
BaeI ACNNNNGTAYC 2 cut(s) 387, 420
BanII GRGCYC 1 cut(s) 553
Bbv12I GWGCWC 1 cut(s) 447
BbvI GCAGC 1 cut(s) 24
BccI CCATC 1 cut(s) 238
BciT130I CCWGG 2 cut(s) 81, 410
BciVI GTATCC 1 cut(s) 415
BcoDI GTCTC 2 cut(s) 352, 504
BfaI CTAG 2 cut(s) 192, 422
BfmI CTRYAG 1 cut(s) 623
BfuAI ACCTGC 2 cut(s) 292, 341
BfuI GTATCC 1 cut(s) 415
BglI GCCNNNNNGGC 1 cut(s) 76
BisI GCNGC 2 cut(s) 38, 71
BlsI GCNGC 2 cut(s) 39, 72
Bme1390I CCNGG 2 cut(s) 81, 410
BmgT120I GGNCC 2 cut(s) 195, 558
BmrFI CCNGG 2 cut(s) 81, 410
BmsI GCATC 2 cut(s) 121, 526
Bpu10I CCTNAGC 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 461
BsaBI GATNNNNATC 2 cut(s) 430, 455
BsaHI GRCGYC 1 cut(s) 398
BsaI GGTCTC 1 cut(s) 352
BsaJI CCNNGG 3 cut(s) 74, 80, 408
BsaWI WCCGGW 1 cut(s) 338
Bsc4I CCNNNNNNNGG 5 cut(s) 80, 127, 415, 546, 567
Bse118I RCCGGY 3 cut(s) 338, 361, 505
Bse1I ACTGG 3 cut(s) 165, 210, 452
Bse8I GATNNNNATC 2 cut(s) 430, 455
BseBI CCWGG 2 cut(s) 81, 410
BseDI CCNNGG 3 cut(s) 74, 80, 408
BseGI GGATG 1 cut(s) 136
BseJI GATNNNNATC 2 cut(s) 430, 455
BseLI CCNNNNNNNGG 5 cut(s) 80, 127, 415, 546, 567
BseMII CTCAG 2 cut(s) 203, 335
BseNI ACTGG 3 cut(s) 165, 210, 452
BseXI GCAGC 1 cut(s) 24
BseYI CCCAGC 1 cut(s) 184
BsgI GTGCAG 1 cut(s) 369
Bsh1236I CGCG 1 cut(s) 370
BshFI GGCC 4 cut(s) 79, 197, 365, 560
BshTI ACCGGT 1 cut(s) 338
BsiHKAI GWGCWC 1 cut(s) 447
BsiSI CCGG 3 cut(s) 339, 362, 506
BslI CCNNNNNNNGG 5 cut(s) 80, 127, 415, 546, 567
BsmAI GTCTC 2 cut(s) 352, 504
BsnI GGCC 4 cut(s) 79, 197, 365, 560
Bso31I GGTCTC 1 cut(s) 352
Bsp1286I GDGCHC 2 cut(s) 447, 553
Bsp143I GATC 4 cut(s) 169, 208, 431, 456
BspACI CCGC 2 cut(s) 71, 176
BspANI GGCC 4 cut(s) 79, 197, 365, 560
BspCNI CTCAG 2 cut(s) 204, 334
BspFNI CGCG 1 cut(s) 370
BspMI ACCTGC 2 cut(s) 292, 341
BspPI GGATC 3 cut(s) 216, 439, 464
BspTNI GGTCTC 1 cut(s) 352
BsrBI CCGCTC 1 cut(s) 178
BsrFI RCCGGY 3 cut(s) 338, 361, 505
BsrI ACTGG 3 cut(s) 165, 210, 452
BssAI RCCGGY 3 cut(s) 338, 361, 505
BssECI CCNNGG 3 cut(s) 74, 80, 408
BssMI GATC 4 cut(s) 169, 208, 431, 456
BssNI GRCGYC 1 cut(s) 398
BssT1I CCWWGG 1 cut(s) 74
Bst2UI CCWGG 2 cut(s) 81, 410
Bst4CI ACNGT 1 cut(s) 620
BstACI GRCGYC 1 cut(s) 398
BstC8I GCNNGC 1 cut(s) 507
BstDEI CTNAG 2 cut(s) 212, 321
BstF5I GGATG 1 cut(s) 136
BstFNI CGCG 1 cut(s) 370
BstKTI GATC 4 cut(s) 172, 211, 434, 459
BstMAI GTCTC 2 cut(s) 352, 504
BstMBI GATC 4 cut(s) 169, 208, 431, 456
BstMWI GCNNNNNNNGC 2 cut(s) 76, 557
BstNI CCWGG 2 cut(s) 81, 410
BstSCI CCNGG 2 cut(s) 79, 408
BstSFI CTRYAG 1 cut(s) 623
BstUI CGCG 1 cut(s) 370
BstV1I GCAGC 1 cut(s) 24
BstX2I RGATCY 1 cut(s) 208
BstXI CCANNNNNNTGG 1 cut(s) 205
BstYI RGATCY 1 cut(s) 208
BsuI GTATCC 1 cut(s) 415
BsuRI GGCC 4 cut(s) 79, 197, 365, 560
BtsCI GGATG 1 cut(s) 136
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 3 cut(s) 141, 172, 445
BveI ACCTGC 2 cut(s) 292, 341
Cac8I GCNNGC 1 cut(s) 507
Cfr10I RCCGGY 3 cut(s) 338, 361, 505
Cfr13I GGNCC 2 cut(s) 195, 558
CseI GACGC 2 cut(s) 326, 359
CspAI ACCGGT 1 cut(s) 338
DdeI CTNAG 2 cut(s) 212, 321
DpnI GATC 4 cut(s) 171, 210, 433, 458
DpnII GATC 4 cut(s) 169, 208, 431, 456
Eco130I CCWWGG 1 cut(s) 74
Eco24I GRGCYC 1 cut(s) 553
Eco31I GGTCTC 1 cut(s) 352
Eco57I CTGAAG 1 cut(s) 448
EcoRI GAATTC 1 cut(s) 238
EcoRII CCWGG 2 cut(s) 79, 408
EcoT14I CCWWGG 1 cut(s) 74
EcoT38I GRGCYC 1 cut(s) 553
ErhI CCWWGG 1 cut(s) 74
FaiI YATR 6 cut(s) 61, 63, 388, 530, 546, 583
FauNDI CATATG 1 cut(s) 61
Fnu4HI GCNGC 2 cut(s) 38, 71
FokI GGATG 1 cut(s) 143
FriOI GRGCYC 1 cut(s) 553
Fsp4HI GCNGC 2 cut(s) 38, 71
FspBI CTAG 2 cut(s) 192, 422
GluI GCNGC 2 cut(s) 38, 71
GsaI CCCAGC 1 cut(s) 188
HaeIII GGCC 4 cut(s) 79, 197, 365, 560
HapII CCGG 3 cut(s) 339, 362, 506
HgaI GACGC 2 cut(s) 326, 359
Hin1I GRCGYC 1 cut(s) 398
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HinfI GANTC 2 cut(s) 22, 605
HpaII CCGG 3 cut(s) 339, 362, 506
Hpy166II GTNNAC 4 cut(s) 109, 142, 600, 616
Hpy188I TCNGA 3 cut(s) 122, 213, 293
Hpy188III TCNNGA 3 cut(s) 307, 357, 485
Hpy8I GTNNAC 4 cut(s) 109, 142, 600, 616
Hpy99I CGWCG 4 cut(s) 320, 375, 397, 400
HpyAV CCTTC 2 cut(s) 288, 324
HpyCH4III ACNGT 1 cut(s) 620
HpyCH4IV ACGT 1 cut(s) 398
HpyCH4V TGCA 3 cut(s) 134, 350, 386
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 557
HpyF3I CTNAG 2 cut(s) 212, 321
HpySE526I ACGT 1 cut(s) 398
Hsp92I GRCGYC 1 cut(s) 398
KroI GCCGGC 1 cut(s) 505
KroNI GCCGGC 1 cut(s) 507
Kzo9I GATC 4 cut(s) 169, 208, 431, 456
LmnI GCTCC 1 cut(s) 188
Lsp1109I GCAGC 1 cut(s) 24
LweI GCATC 2 cut(s) 121, 526
MaeI CTAG 2 cut(s) 192, 422
MaeII ACGT 1 cut(s) 398
MaeIII GTNAC 1 cut(s) 435
MalI GATC 4 cut(s) 171, 210, 433, 458
MbiI CCGCTC 1 cut(s) 178
MboI GATC 4 cut(s) 169, 208, 431, 456
MflI RGATCY 1 cut(s) 208
MhlI GDGCHC 2 cut(s) 447, 553
MluCI AATT 1 cut(s) 238
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 1 cut(s) 496
MnlI CCTC 3 cut(s) 121, 368, 376
MroNI GCCGGC 1 cut(s) 505
MseI TTAA 1 cut(s) 12
MspI CCGG 3 cut(s) 339, 362, 506
MspR9I CCNGG 2 cut(s) 81, 410
MvaI CCWGG 2 cut(s) 81, 410
MvnI CGCG 1 cut(s) 370
MwoI GCNNNNNNNGC 2 cut(s) 76, 557
NaeI GCCGGC 1 cut(s) 507
NdeI CATATG 1 cut(s) 61
NdeII GATC 4 cut(s) 169, 208, 431, 456
NgoMIV GCCGGC 1 cut(s) 505
PcsI WCGNNNNNNNCGW 1 cut(s) 239
PdiI GCCGGC 1 cut(s) 507
PfeI GAWTC 1 cut(s) 605
PflFI GACNNNGTC 1 cut(s) 397
PinAI ACCGGT 1 cut(s) 338
PkrI GCNGC 2 cut(s) 39, 72
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
Psp6I CCWGG 2 cut(s) 79, 408
PspFI CCCAGC 1 cut(s) 184
PspGI CCWGG 2 cut(s) 79, 408
PspPI GGNCC 2 cut(s) 195, 558
PsuI RGATCY 1 cut(s) 208
PsyI GACNNNGTC 1 cut(s) 397
SaqAI TTAA 1 cut(s) 12
SatI GCNGC 2 cut(s) 38, 71
Sau3AI GATC 4 cut(s) 169, 208, 431, 456
Sau96I GGNCC 2 cut(s) 195, 558
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 2 cut(s) 81, 410
SduI GDGCHC 2 cut(s) 447, 553
SetI ASST 8 cut(s) 18, 193, 299, 306, 355, 401, 483, 615
SfaNI GCATC 2 cut(s) 121, 526
SfcI CTRYAG 1 cut(s) 623
SmlI CTYRAG 1 cut(s) 440
SmoI CTYRAG 1 cut(s) 440
Sse9I AATT 1 cut(s) 238
SsiI CCGC 2 cut(s) 71, 176
SspMI CTAG 2 cut(s) 192, 422
StyD4I CCNGG 2 cut(s) 79, 408
StyI CCWWGG 1 cut(s) 74
TaaI ACNGT 1 cut(s) 620
TaiI ACGT 1 cut(s) 401
TaqI TCGA 4 cut(s) 223, 242, 356, 373
TasI AATT 1 cut(s) 238
TauI GCSGC 1 cut(s) 73
TfiI GAWTC 1 cut(s) 605
Tru1I TTAA 1 cut(s) 12
Tru9I TTAA 1 cut(s) 12
TscAI CASTG 3 cut(s) 141, 172, 452
TseI GCWGC 1 cut(s) 37
TspDTI ATGAA 1 cut(s) 48
TspGWI ACGGA 1 cut(s) 391
TspRI CASTG 3 cut(s) 141, 172, 452
Tth111I GACNNNGTC 1 cut(s) 397
XapI RAATTY 1 cut(s) 238
XspI CTAG 2 cut(s) 192, 422
ZraI GACGTC 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.