Rroxscaffold_5G00342740

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004840
Physical Location & Seq
Reverse (-)
11873309 .. 11877954
4646 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00342740.1

Sequence Viewer

Length: 3528 bp
ATGAAAGTCGAGGGGTGCAGAAGACTTGCGGTAATAGTGGTAAGCAATGAAGACAGCAATTCAAGAAATAAGATTACATTCAGGCGATTGAAACATTTGAAACTATCTAGTCTACCAGGACTGAAAGGCTTTTGCTCTGAAAATTGCATTGCTAAATTCCCGTCCTTGGAAACTTCAAGGATGAGCAACCGCCTCAAATTGAAGATTTTCCCAATTCATAACCAAATGCTACGGCTAACCAACGAAGAGGAAGACACAGATGTCGAACGTTGGGAGGATGAAATGAATCCGGCAATACATGCAGAAATACATGGAGTTGGGGATGGGATCGTCCCACATTTAAGAGGGTTGAATGTTTATGAGATGCGGAAGCTGATGAATCTTGGGGAGGATAGCTTCGGACCAGCATGCCCATATTTTTCAAATCTGGAAATTCTGAAGACAGATGACTTTGATAGCTTAAAGAACTTAAGATCAACCGCGATATTCTTCAAGAATCTAACAACTTTGCAAGTAAGTCGTTGTAAGGGATTGAAATACTTGACATCTTTCTCCATCTTCAAAAGTTTGATGCAACTCACAAACCTAGAAGTTCAGGATTGTGAGGAAATGATAGAAATAGTGGGGAGCAATGAAAATGAGGATTCAGAAAATGATATTGAATTCAGGGTGTTAAAACATTTGGAACTTTCTGATCTACCAAGTCTAAGAGGCTTTTGCTCAGCAACTCGCACTGTCAAATTCCCATCCTTGGAAAATTTATCGATGAGAAGTTGTACTCAATTAGAGGCATTCATCTTTGACCCTACGGATAAAAGTAATACAATTGGCAAGGATATCAAGGACACAAACTCAAATGAAAATATTGGGAATGGAGCAGTGCAACAATTCCTTTTTGACAACAAGGTTGAATTTCCGAGGTTGAAGAGTTTGTCTATTGCGGGTCTAACAAAGTTGACCGCAATATGGCACAGTCAACTTTCTCCAAACTCCTTTAGTAGACTGAAAGACCTTCAAGTACATGGATGTGGAAATCTAATAAATATATTTTTGCCCAGTACTATGGGAAGATTGAATGCTCCTGAGACCTTATTGATAAAGGAGTGCAAGTCTGTACAAGTTGTATTTAAACTCGGAGGCATTGGTGTTAGAGGAGTGCATGACACGTTAGCAGCAACTCAGCTGAGATCTTTTGACTTTGAAACTCTGGATTCGGTAGAAATTGATTCTTGTGAGAGTTTGAAAAACATTTTCCCAGTTTCAGTGGCTAGAAATCTCCACCAGTTAAAAAGGCTAATAGTGAAGAGTTGTGGAGTTCAGGAAATTGTCGCAAAGGAAGAGTCACTACAAACAATACCTAAGTTTGTGTTTCCAAAAATAAGAAATGTGATTTTTTGTGATTTGTCCCAACTCACGAATTTCTACCCAGGAATGCATGTGTCTAGCTGGCCATCACTTAATGAGTTGAGGGTGGTACAATGTGCTAAAGTCAAAGTGTTTGTTGCGGAAATCTCAAGTTTTGAAGGACATAATGAGCCGGAACGTCTCAGTGTTCTTATCCCGCAATCTCTCTTTTTAATTGAGAAGGTAAAAAATGGTTTGCCTAACTTGACAAGCTTGATGGTTCACCGGTGTGATGGTTTAAATTTCTTACTGGTATCTTCCATGGCAAGAAGTCTTGTAAAACTCAAACATCTTGTGATATCTGAATGTCAAAGAATGGAAGAAGTATTGACAAAAGAACACAGTGAAGAAAACAAGAATGACATGTTTCCTGATTTAAAAGAATTGGAGCTAAAAGATCTTCCTCATCTTGTTAGATTCTGCTCAGGAAATTATTTTGAATTTGCATCTATGGAGAGATTGCAATTAGAAAAGTGTGCTAAATTGGAGACATTCATCTGTAATCCCTCGAGTAACAGTATTGCAAGCCAAAAAGAAATGGAAAGAGTGGACAAGAATGAGAACCTTGAGACAGTAGTACCTTGCTTTCTTTTTGACAAAAAGGTAGGGCTTCCAAGGTTGGAGAGATTGATTATCAATGAACTACCGAAGCTCACAACAATTTGGCACACCCAACTTGCTCCAAACTCTTTCCACAGACTGGAAGACGTTACAGTGCAACAATGCAATGGTCTTAAATATATTTTTCAAGTCTCCATCATAGCCGGTGTTTTTCAGCAGCTACAAAAGTTGATAGTAAAGGAATGTGTAGTGGAGGAAATAGTTTTTCAGGAAGAGGGCGTAGAAAAATTAATGCCTGATCAGTTCTTCCCAGAGCTAACATGTTTGGAATTTACAAGTCTATTCCAACTTGAGAGATTCTATCTAAAGATGCCTGCTATCAACTGGCCACTAAAAACATTAGTGTTGTCTGATTGTCAGAAAGTGGATATCTTTACCGCGGAAGTTTCAGGCCTTCAGAAAACACATGACTTGGGGAATCTGCGTACTCGGGTTAAACAATATCCTGTTCTATTTGAGAAGAGTTCATTTTCCAAACTGGAAGTTTTGACCTTCGGCCCCGCGAACATCTGGTACGGTCTACCTGCCCATGAGAAAGCTCTTTTTCCTGCTACCAGTACAGATGGGTCCCTTCCACATTTGAGAACGTTGAGGCTACATGGAATGGAGAAGTTTATGCGTCTCTGGGAGAACACCGAACCTGCAGCTAGCTCAGCTTTTTCAAAGATGGATACTCTTGAGGTGAAGAACTCTGGATTGACTAATCTAGAGTTGTCTGCAATATCCTTCCAGAATCTAACAATTTTAGAAGTAACCTTTTGCTGGCGATTGCAATATTTGACCACTTATTCGGTAGCTCGAAGTCTCGTGCACCTCAAAACTTTGAAAGTTGACGAATGTAAAAATATGAAAGAAATCTTCACAAGCGAGGGAATGAGAGAAGATGCAAGTGAATTTGAGATTGAGTTCAGTCAGTTGCAAAGTTTGGAACTTAGTGATTTAGTAAGTTTGGAACGGTTTTGCTCGAGCAATTGCAGAGTGAAGGTCCCAATCTTGGGAACTTTAAAGGTGAATTGGTGCCCGTTCAATTTGAAGATTTCCTCTGATAAGGTACTAGAAGTTGATGGTGTCCAAACTAAATCATGGTCGCAAAGCATGTCTATGCTTGAACAGGACAGCAGACGTTTGCGGGAAGAATTAAGGGCCAAAGAAGGTAACAGCCTCCCGGAGACTCCGGTTGTTCTCGCGCTGCCTGAGACTGGTCCTTCTCGCTCGTTGACTGCCAAAGGAACTGCTGATCTTCCGGTGGAGGTGATAGCCCAGCTTAATGCTCTGCTTGGAATTGATCTGGCAACTCCTGCCGTGGACAAGAAGAGCTCTTCTAACCACATAGTGACTGAGCTAACTGCCCAACTGGAGGCAAGAAGGTCTGAGCTTATTGCCGGCAGCTCTCAAGTTGAGGCGGAATGTCAAGAAGTCATGGGCCGGGAGGCGGAACTTGCTAACATAAGACAACTAATCAAACTGCTTGAAGCTCGGGCCGCTACTCTTGAGGCGCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1175

Amino Acids

132.97

Weight (kDa)

6.0

Isoelectric Point (pI)

45.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 160 - 264 4.2e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 311 - 385 4.9e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 397 - 438 1e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 532 - 633 1.8e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 672 - 737 6e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 910 - 1015 6.1e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 260
Acc36I ACCTGC 2 cut(s) 2557, 2674
AccB1I GGYRCC 1 cut(s) 3042
AccB7I CCANNNNNTGG 1 cut(s) 2104
AccI GTMKAC 3 cut(s) 112, 1000, 2545
AccII CGCG 5 cut(s) 482, 2405, 2528, 3212, 3523
AclI AACGTT 2 cut(s) 268, 2612
AclWI GGATC 1 cut(s) 335
AcoI YGGCCR 2 cut(s) 1448, 2351
AcuI CTGAAG 2 cut(s) 458, 2405
AdeI CACNNNGTG 1 cut(s) 3358
AflII CTTAAG 1 cut(s) 469
AflIII ACRYGT 3 cut(s) 1164, 1767, 2285
AgeI ACCGGT 1 cut(s) 1629
AjnI CCWGG 2 cut(s) 115, 1426
AleI CACNNNNGTG 1 cut(s) 1632
Alw21I GWGCWC 2 cut(s) 2838, 3344
Alw26I GTCTC 9 cut(s) 1079, 1550, 1886, 1967, 2161, 2651, 2834, 3188, 3215
Alw44I GTGCAC 1 cut(s) 2834
AlwI GGATC 1 cut(s) 335
Ama87I CYCGRG 4 cut(s) 1912, 2454, 2989, 3501
AoxI GGCC 7 cut(s) 1448, 2351, 2416, 2521, 3168, 3448, 3504
ApaLI GTGCAC 1 cut(s) 2834
ApeKI GCWGC 5 cut(s) 1172, 2182, 2669, 3214, 3411
ArsI GACNNNNNNTTYG 4 cut(s) 1325, 1357, 2797, 2829
AseI ATTAAT 1 cut(s) 2255
AsiGI ACCGGT 1 cut(s) 1629
Asp700I GAANNNNTTC 1 cut(s) 206
AspLEI GCGC 3 cut(s) 3214, 3523, 3525
AspS9I GGNCC 8 cut(s) 401, 2522, 2592, 3010, 3168, 3227, 3448, 3504
AsuC2I CCSGG 2 cut(s) 3191, 3452
AsuHPI GGTGA 4 cut(s) 1619, 2719, 3046, 3289
AsuNHI GCTAGC 1 cut(s) 2672
AvaI CYCGRG 4 cut(s) 1912, 2454, 2989, 3501
AvaII GGWCC 4 cut(s) 401, 2592, 3010, 3227
BaeGI GKGCMC 2 cut(s) 2838, 3047
BaeI ACNNNNGTAYC 2 cut(s) 1965, 1998
BalI TGGCCA 2 cut(s) 1450, 2353
BanI GGYRCC 1 cut(s) 3042
BanII GRGCYC 1 cut(s) 3344
BarI GAAGNNNNNNTAC 2 cut(s) 1329, 1361
BauI CACGAG 1 cut(s) 2831
BbsI GAAGAC 5 cut(s) 28, 57, 258, 446, 2115
Bbv12I GWGCWC 2 cut(s) 2838, 3344
BbvI GCAGC 5 cut(s) 1184, 2194, 2681, 3201, 3423
BceAI ACGGC 2 cut(s) 248, 3311
BcgI CGANNNNNNTGC 2 cut(s) 500, 534
BciT130I CCWGG 2 cut(s) 117, 1428
BciVI GTATCC 1 cut(s) 2689
BclI TGATCA 1 cut(s) 2263
BcnI CCSGG 2 cut(s) 3191, 3452
BcoDI GTCTC 9 cut(s) 1079, 1550, 1886, 1967, 2161, 2651, 2834, 3188, 3215
BfaI CTAG 7 cut(s) 108, 587, 1269, 1443, 2673, 2732, 3080
BfmI CTRYAG 1 cut(s) 2667
BfrI CTTAAG 1 cut(s) 469
BfuAI ACCTGC 2 cut(s) 2557, 2674
BfuI GTATCC 1 cut(s) 2689
BglII AGATCT 2 cut(s) 1187, 1801
BisI GCNGC 6 cut(s) 1173, 2183, 2670, 3215, 3412, 3507
BlpI GCTNAGC 2 cut(s) 721, 2677
BlsI GCNGC 6 cut(s) 1174, 2184, 2671, 3216, 3413, 3508
BmcAI AGTACT 1 cut(s) 1060
Bme1390I CCNGG 4 cut(s) 117, 1428, 3191, 3452
Bme18I GGWCC 4 cut(s) 401, 2592, 3010, 3227
BmeT110I CYCGRG 4 cut(s) 1912, 2454, 2989, 3501
BmgT120I GGNCC 8 cut(s) 401, 2522, 2592, 3010, 3168, 3227, 3448, 3504
BmiI GGNNCC 5 cut(s) 2524, 2593, 2594, 3012, 3044
BmrFI CCNGG 4 cut(s) 117, 1428, 3191, 3452
BmrI ACTGGG 2 cut(s) 1050, 1250
BmsI GCATC 5 cut(s) 354, 561, 1859, 2325, 2899
BmtI GCTAGC 1 cut(s) 2676
BmuI ACTGGG 2 cut(s) 1050, 1250
BpiI GAAGAC 5 cut(s) 28, 57, 258, 446, 2115
BpmI CTGGAG 1 cut(s) 3401
Bpu10I CCTNAGC 1 cut(s) 1828
Bpu1102I GCTNAGC 2 cut(s) 721, 2677
BpuEI CTTGAG 5 cut(s) 1498, 1991, 2336, 2723, 3402
BpuMI CCSGG 2 cut(s) 3191, 3452
Bsa29I ATCGAT 1 cut(s) 764
BsaI GGTCTC 1 cut(s) 1079
BsaJI CCNNGG 8 cut(s) 165, 750, 917, 1426, 1665, 2018, 2403, 3327
BsaWI WCCGGW 3 cut(s) 1629, 3199, 3268
BsaXI ACNNNNNCTCC 2 cut(s) 1094, 1124
Bse118I RCCGGY 3 cut(s) 1629, 2168, 3407
Bse3DI GCAATG 4 cut(s) 52, 147, 637, 2137
BseBI CCWGG 2 cut(s) 117, 1428
BseCI ATCGAT 1 cut(s) 764
BseDI CCNNGG 8 cut(s) 165, 750, 917, 1426, 1665, 2018, 2403, 3327
BseGI GGATG 5 cut(s) 186, 283, 328, 746, 1031
BseMI GCAATG 4 cut(s) 52, 147, 637, 2137
BsePI GCGCGC 1 cut(s) 3521
BseRI GAGGAG 1 cut(s) 1167
BseSI GKGCMC 2 cut(s) 2838, 3047
BseXI GCAGC 5 cut(s) 1184, 2194, 2681, 3201, 3423
BseYI CCCAGC 1 cut(s) 3285
BsgI GTGCAG 1 cut(s) 37
Bsh1236I CGCG 5 cut(s) 482, 2405, 2528, 3212, 3523
BshFI GGCC 7 cut(s) 1450, 2353, 2418, 2523, 3170, 3450, 3506
BshNI GGYRCC 1 cut(s) 3042
BshTI ACCGGT 1 cut(s) 1629
BshVI ATCGAT 1 cut(s) 764
BsiHKAI GWGCWC 2 cut(s) 2838, 3344
BsiHKCI CYCGRG 4 cut(s) 1912, 2454, 2989, 3501
BsiSI CCGG 9 cut(s) 290, 1538, 1630, 2169, 3191, 3200, 3269, 3408, 3451
BslFI GGGAC 4 cut(s) 317, 1390, 2578, 2996
BsmAI GTCTC 9 cut(s) 1079, 1550, 1886, 1967, 2161, 2651, 2834, 3188, 3215
BsmBI CGTCTC 2 cut(s) 1550, 2651
BsmFI GGGAC 4 cut(s) 317, 1390, 2578, 2996
BsmI GAATGC 3 cut(s) 791, 1081, 1437
BsnI GGCC 7 cut(s) 1450, 2353, 2418, 2523, 3170, 3450, 3506
Bso31I GGTCTC 1 cut(s) 1079
BsoBI CYCGRG 4 cut(s) 1912, 2454, 2989, 3501
Bsp1286I GDGCHC 3 cut(s) 2838, 3047, 3344
Bsp1407I TGTACA 1 cut(s) 1114
Bsp143I GATC 8 cut(s) 327, 473, 694, 1187, 1801, 2263, 3262, 3310
Bsp1720I GCTNAGC 2 cut(s) 721, 2677
Bsp19I CCATGG 1 cut(s) 1665
BspANI GGCC 7 cut(s) 1450, 2353, 2418, 2523, 3170, 3450, 3506
BspDI ATCGAT 1 cut(s) 764
BspFNI CGCG 5 cut(s) 482, 2405, 2528, 3212, 3523
BspLI GGNNCC 5 cut(s) 2524, 2593, 2594, 3012, 3044
BspMAI CTGCAG 1 cut(s) 2671
BspMI ACCTGC 2 cut(s) 2557, 2674
BspOI GCTAGC 1 cut(s) 2676
BspPI GGATC 1 cut(s) 335
BspQI GCTCTTC 2 cut(s) 3332, 3349
BspT107I GGYRCC 1 cut(s) 3042
BspTI CTTAAG 1 cut(s) 469
BspTNI GGTCTC 1 cut(s) 1079
BsrDI GCAATG 4 cut(s) 52, 147, 637, 2137
BsrFI RCCGGY 3 cut(s) 1629, 2168, 3407
BsrGI TGTACA 1 cut(s) 1114
BssAI RCCGGY 3 cut(s) 1629, 2168, 3407
BssECI CCNNGG 8 cut(s) 165, 750, 917, 1426, 1665, 2018, 2403, 3327
BssHII GCGCGC 1 cut(s) 3521
BssMI GATC 8 cut(s) 327, 473, 694, 1187, 1801, 2263, 3262, 3310
BssSI CACGAG 1 cut(s) 2831
BssT1I CCWWGG 4 cut(s) 165, 750, 1665, 2018
Bst2BI CACGAG 1 cut(s) 2831
Bst2UI CCWGG 2 cut(s) 117, 1428
Bst4CI ACNGT 8 cut(s) 736, 974, 1748, 1922, 1978, 2119, 2543, 2982
Bst6I CTCTTC 8 cut(s) 240, 920, 1298, 1332, 2232, 2480, 3332, 3349
BstAFI CTTAAG 1 cut(s) 469
BstAPI GCANNNNNTGC 2 cut(s) 299, 3323
BstAUI TGTACA 1 cut(s) 1114
BstC8I GCNNGC 8 cut(s) 409, 1448, 1930, 2340, 2674, 2789, 3409, 3523
BstDSI CCRYGG 3 cut(s) 1665, 2403, 3327
BstF5I GGATG 5 cut(s) 186, 283, 328, 746, 1031
BstFNI CGCG 5 cut(s) 482, 2405, 2528, 3212, 3523
BstHHI GCGC 3 cut(s) 3214, 3523, 3525
BstKTI GATC 8 cut(s) 330, 476, 697, 1190, 1804, 2266, 3265, 3313
BstMAI GTCTC 9 cut(s) 1079, 1550, 1886, 1967, 2161, 2651, 2834, 3188, 3215
BstMBI GATC 8 cut(s) 327, 473, 694, 1187, 1801, 2263, 3262, 3310
BstMWI GCNNNNNNNGC 5 cut(s) 299, 2678, 3323, 3464, 3506
BstNI CCWGG 2 cut(s) 117, 1428
BstNSI RCATGY 6 cut(s) 302, 411, 1439, 1771, 2289, 3124
BstSCI CCNGG 4 cut(s) 115, 1426, 3189, 3450
BstSFI CTRYAG 1 cut(s) 2667
BstSLI GKGCMC 2 cut(s) 2838, 3047
BstUI CGCG 5 cut(s) 482, 2405, 2528, 3212, 3523
BstV1I GCAGC 5 cut(s) 1184, 2194, 2681, 3201, 3423
BstV2I GAAGAC 5 cut(s) 28, 57, 258, 446, 2115
BstX2I RGATCY 2 cut(s) 1187, 1801
BstXI CCANNNNNNTGG 1 cut(s) 1063
BstYI RGATCY 2 cut(s) 1187, 1801
Bsu15I ATCGAT 1 cut(s) 764
BsuI GTATCC 1 cut(s) 2689
BsuRI GGCC 7 cut(s) 1450, 2353, 2418, 2523, 3170, 3450, 3506
BsuTUI ATCGAT 1 cut(s) 764
BtgI CCRYGG 3 cut(s) 1665, 2403, 3327
BtsCI GGATG 5 cut(s) 186, 283, 328, 746, 1031
BtsI GCAGTG 1 cut(s) 885
BtsIMutI CAGTG 6 cut(s) 732, 885, 1269, 1555, 1753, 2124
BveI ACCTGC 2 cut(s) 2557, 2674
Cac8I GCNNGC 8 cut(s) 409, 1448, 1930, 2340, 2674, 2789, 3409, 3523
CfoI GCGC 3 cut(s) 3214, 3523, 3525
Cfr10I RCCGGY 3 cut(s) 1629, 2168, 3407
Cfr13I GGNCC 8 cut(s) 401, 2522, 2592, 3010, 3168, 3227, 3448, 3504
Cfr42I CCGCGG 1 cut(s) 2406
ClaI ATCGAT 1 cut(s) 764
CseI GACGC 1 cut(s) 2633
CspAI ACCGGT 1 cut(s) 1629
DpnI GATC 8 cut(s) 329, 475, 696, 1189, 1803, 2265, 3264, 3312
DpnII GATC 8 cut(s) 327, 473, 694, 1187, 1801, 2263, 3262, 3310
DraI TTTAAA 4 cut(s) 1129, 1644, 1782, 3030
DraIII CACNNNGTG 1 cut(s) 3358
DrdI GACNNNNNNGTC 1 cut(s) 260
DseDI GACNNNNNNGTC 1 cut(s) 260
EaeI YGGCCR 2 cut(s) 1448, 2351
Eam1104I CTCTTC 8 cut(s) 240, 920, 1298, 1332, 2232, 2480, 3332, 3349
EarI CTCTTC 8 cut(s) 240, 920, 1298, 1332, 2232, 2480, 3332, 3349
EciI GGCGGA 2 cut(s) 3443, 3473
Ecl136II GAGCTC 1 cut(s) 3342
Eco130I CCWWGG 4 cut(s) 165, 750, 1665, 2018
Eco147I AGGCCT 1 cut(s) 2418
Eco24I GRGCYC 1 cut(s) 3344
Eco31I GGTCTC 1 cut(s) 1079
Eco32I GATATC 3 cut(s) 838, 1704, 2395
Eco47I GGWCC 4 cut(s) 401, 2592, 3010, 3227
Eco53kI GAGCTC 1 cut(s) 3342
Eco57I CTGAAG 2 cut(s) 458, 2405
Eco88I CYCGRG 4 cut(s) 1912, 2454, 2989, 3501
EcoICRI GAGCTC 1 cut(s) 3342
EcoO109I RGGNCCY 2 cut(s) 2592, 3010
EcoRI GAATTC 1 cut(s) 662
EcoRII CCWGG 2 cut(s) 115, 1426
EcoRV GATATC 3 cut(s) 838, 1704, 2395
EcoT14I CCWWGG 4 cut(s) 165, 750, 1665, 2018
EcoT22I ATGCAT 1 cut(s) 1437
EcoT38I GRGCYC 1 cut(s) 3344
ErhI CCWWGG 4 cut(s) 165, 750, 1665, 2018
Esp3I CGTCTC 2 cut(s) 1550, 2651
FaqI GGGAC 4 cut(s) 317, 1390, 2578, 2996
FauI CCCGC 4 cut(s) 934, 1569, 2533, 3147
FbaI TGATCA 1 cut(s) 2263
FblI GTMKAC 3 cut(s) 112, 1000, 2545
Fnu4HI GCNGC 6 cut(s) 1173, 2183, 2670, 3215, 3412, 3507
FokI GGATG 5 cut(s) 193, 290, 335, 733, 1038
FriOI GRGCYC 1 cut(s) 3344
Fsp4HI GCNGC 6 cut(s) 1173, 2183, 2670, 3215, 3412, 3507
FspBI CTAG 7 cut(s) 108, 587, 1269, 1443, 2673, 2732, 3080
GlaI GCGC 3 cut(s) 3213, 3522, 3524
GluI GCNGC 6 cut(s) 1173, 2183, 2670, 3215, 3412, 3507
GsaI CCCAGC 1 cut(s) 3289
GsuI CTGGAG 1 cut(s) 3401
HaeIII GGCC 7 cut(s) 1450, 2353, 2418, 2523, 3170, 3450, 3506
HapII CCGG 9 cut(s) 290, 1538, 1630, 2169, 3191, 3200, 3269, 3408, 3451
HgaI GACGC 1 cut(s) 2633
HhaI GCGC 3 cut(s) 3214, 3523, 3525
Hin6I GCGC 3 cut(s) 3212, 3521, 3523
HinP1I GCGC 3 cut(s) 3212, 3521, 3523
HincII GTYRAC 4 cut(s) 957, 977, 2857, 3243
HindII GTYRAC 4 cut(s) 957, 977, 2857, 3243
HindIII AAGCTT 1 cut(s) 1615
HpaII CCGG 9 cut(s) 290, 1538, 1630, 2169, 3191, 3200, 3269, 3408, 3451
HphI GGTGA 4 cut(s) 1619, 2719, 3046, 3289
HpyCH4III ACNGT 8 cut(s) 736, 974, 1748, 1922, 1978, 2119, 2543, 2982
HpyCH4IV ACGT 6 cut(s) 268, 1166, 1543, 2112, 2612, 3148
HpyF10VI GCNNNNNNNGC 5 cut(s) 299, 2678, 3323, 3464, 3506
HpySE526I ACGT 6 cut(s) 268, 1166, 1543, 2112, 2612, 3148
HspAI GCGC 3 cut(s) 3212, 3521, 3523
KflI GGGWCCC 1 cut(s) 2592
KroI GCCGGC 1 cut(s) 3407
KroNI GCCGGC 1 cut(s) 3409
Ksp22I TGATCA 1 cut(s) 2263
KspI CCGCGG 1 cut(s) 2406
Kzo9I GATC 8 cut(s) 327, 473, 694, 1187, 1801, 2263, 3262, 3310
LguI GCTCTTC 2 cut(s) 3332, 3349
LmnI GCTCC 5 cut(s) 627, 875, 1084, 1792, 2089
Lsp1109I GCAGC 5 cut(s) 1184, 2194, 2681, 3201, 3423
LweI GCATC 5 cut(s) 354, 561, 1859, 2325, 2899
MaeI CTAG 7 cut(s) 108, 587, 1269, 1443, 2673, 2732, 3080
MaeII ACGT 6 cut(s) 268, 1166, 1543, 2112, 2612, 3148
MaeIII GTNAC 6 cut(s) 1341, 1916, 2113, 2776, 3179, 3358
MalI GATC 8 cut(s) 329, 475, 696, 1189, 1803, 2265, 3264, 3312
MboI GATC 8 cut(s) 327, 473, 694, 1187, 1801, 2263, 3262, 3310
MfeI CAATTG 2 cut(s) 825, 2995
MflI RGATCY 2 cut(s) 1187, 1801
MhlI GDGCHC 3 cut(s) 2838, 3047, 3344
MlsI TGGCCA 2 cut(s) 1450, 2353
MluNI TGGCCA 2 cut(s) 1450, 2353
MlyI GAGTC 2 cut(s) 1349, 3190
MmeI TCCRAC 2 cut(s) 2004, 2335
Mox20I TGGCCA 2 cut(s) 1450, 2353
Mph1103I ATGCAT 1 cut(s) 1437
MroNI GCCGGC 1 cut(s) 3407
MroXI GAANNNNTTC 1 cut(s) 206
MscI TGGCCA 2 cut(s) 1450, 2353
MslI CAYNNNNRTG 3 cut(s) 1026, 1632, 3125
Msp20I TGGCCA 2 cut(s) 1450, 2353
MspA1I CMGCKG 2 cut(s) 1183, 2405
MspCI CTTAAG 1 cut(s) 469
MspI CCGG 9 cut(s) 290, 1538, 1630, 2169, 3191, 3200, 3269, 3408, 3451
MspR9I CCNGG 4 cut(s) 117, 1428, 3191, 3452
MunI CAATTG 2 cut(s) 825, 2995
Mva1269I GAATGC 3 cut(s) 791, 1081, 1437
MvaI CCWGG 2 cut(s) 117, 1428
MvnI CGCG 5 cut(s) 482, 2405, 2528, 3212, 3523
MwoI GCNNNNNNNGC 5 cut(s) 299, 2678, 3323, 3464, 3506
NaeI GCCGGC 1 cut(s) 3409
NciI CCSGG 2 cut(s) 3191, 3452
NcoI CCATGG 1 cut(s) 1665
NdeII GATC 8 cut(s) 327, 473, 694, 1187, 1801, 2263, 3262, 3310
NgoMIV GCCGGC 1 cut(s) 3407
NheI GCTAGC 1 cut(s) 2672
NlaIV GGNNCC 5 cut(s) 2524, 2593, 2594, 3012, 3044
NmuCI GTSAC 2 cut(s) 1341, 3358
NsiI ATGCAT 1 cut(s) 1437
NspI RCATGY 6 cut(s) 302, 411, 1439, 1771, 2289, 3124
OliI CACNNNNGTG 1 cut(s) 1632
PaeI GCATGC 1 cut(s) 411
PaeR7I CTCGAG 2 cut(s) 1912, 2989
PauI GCGCGC 1 cut(s) 3521
PceI AGGCCT 1 cut(s) 2418
PciI ACATGT 2 cut(s) 1767, 2285
PciSI GCTCTTC 2 cut(s) 3332, 3349
PctI GAATGC 3 cut(s) 791, 1081, 1437
PdiI GCCGGC 1 cut(s) 3409
PdmI GAANNNNTTC 1 cut(s) 206
PflMI CCANNNNNTGG 1 cut(s) 2104
PfoI TCCNGGA 1 cut(s) 3189
PinAI ACCGGT 1 cut(s) 1629
PkrI GCNGC 6 cut(s) 1174, 2184, 2671, 3216, 3413, 3508
PleI GAGTC 2 cut(s) 1348, 3190
PpsI GAGTC 2 cut(s) 1348, 3190
PpuMI RGGWCCY 2 cut(s) 2592, 3010
PscI ACATGT 2 cut(s) 1767, 2285
PshBI ATTAAT 1 cut(s) 2255
Psp124BI GAGCTC 1 cut(s) 3344
Psp1406I AACGTT 2 cut(s) 268, 2612
Psp5II RGGWCCY 2 cut(s) 2592, 3010
Psp6I CCWGG 2 cut(s) 115, 1426
PspFI CCCAGC 1 cut(s) 3285
PspGI CCWGG 2 cut(s) 115, 1426
PspN4I GGNNCC 5 cut(s) 2524, 2593, 2594, 3012, 3044
PspPI GGNCC 8 cut(s) 401, 2522, 2592, 3010, 3168, 3227, 3448, 3504
PspPPI RGGWCCY 2 cut(s) 2592, 3010
PspXI VCTCGAGB 2 cut(s) 1912, 2989
PsrI GAACNNNNNNTAC 2 cut(s) 2522, 2554
PstI CTGCAG 1 cut(s) 2671
PsuI RGATCY 2 cut(s) 1187, 1801
PteI GCGCGC 1 cut(s) 3521
PvuII CAGCTG 1 cut(s) 1183
RseI CAYNNNNRTG 3 cut(s) 1026, 1632, 3125
SacI GAGCTC 1 cut(s) 3344
SacII CCGCGG 1 cut(s) 2406
SapI GCTCTTC 2 cut(s) 3332, 3349
SatI GCNGC 6 cut(s) 1173, 2183, 2670, 3215, 3412, 3507
Sau3AI GATC 8 cut(s) 327, 473, 694, 1187, 1801, 2263, 3262, 3310
Sau96I GGNCC 8 cut(s) 401, 2522, 2592, 3010, 3168, 3227, 3448, 3504
ScaI AGTACT 1 cut(s) 1060
SchI GAGTC 2 cut(s) 1349, 3190
ScrFI CCNGG 4 cut(s) 117, 1428, 3191, 3452
SduI GDGCHC 3 cut(s) 2838, 3047, 3344
SfaNI GCATC 5 cut(s) 354, 561, 1859, 2325, 2899
SfcI CTRYAG 1 cut(s) 2667
Sfr274I CTCGAG 2 cut(s) 1912, 2989
Sfr303I CCGCGG 1 cut(s) 2406
SgrAI CRCCGGYG 1 cut(s) 1629
SgrBI CCGCGG 1 cut(s) 2406
SinI GGWCC 4 cut(s) 401, 2592, 3010, 3227
SlaI CTCGAG 2 cut(s) 1912, 2989
SmiMI CAYNNNNRTG 3 cut(s) 1026, 1632, 3125
SmlI CTYRAG 9 cut(s) 469, 1513, 1912, 1970, 2315, 2702, 2989, 3417, 3515
SmoI CTYRAG 9 cut(s) 469, 1513, 1912, 1970, 2315, 2702, 2989, 3417, 3515
SphI GCATGC 1 cut(s) 411
SseBI AGGCCT 1 cut(s) 2418
SspI AATATT 2 cut(s) 865, 2801
SspMI CTAG 7 cut(s) 108, 587, 1269, 1443, 2673, 2732, 3080
SstI GAGCTC 1 cut(s) 3344
StuI AGGCCT 1 cut(s) 2418
StyD4I CCNGG 4 cut(s) 115, 1426, 3189, 3450
StyI CCWWGG 4 cut(s) 165, 750, 1665, 2018
TaaI ACNGT 8 cut(s) 736, 974, 1748, 1922, 1978, 2119, 2543, 2982
TaiI ACGT 6 cut(s) 271, 1169, 1546, 2115, 2615, 3151
TaqI TCGA 6 cut(s) 9, 264, 764, 1913, 2824, 2990
TatI WGTACW 5 cut(s) 776, 1018, 1058, 1114, 2582
TauI GCSGC 1 cut(s) 3509
TscAI CASTG 6 cut(s) 739, 885, 1269, 1555, 1753, 2124
TseFI GTSAC 2 cut(s) 1341, 3358
TseI GCWGC 5 cut(s) 1172, 2182, 2669, 3214, 3411
Tsp45I GTSAC 2 cut(s) 1341, 3358
TspGWI ACGGA 1 cut(s) 824
TspRI CASTG 6 cut(s) 739, 885, 1269, 1555, 1753, 2124
Van91I CCANNNNNTGG 1 cut(s) 2104
Vha464I CTTAAG 1 cut(s) 469
VneI GTGCAC 1 cut(s) 2834
VpaK11BI GGWCC 4 cut(s) 401, 2592, 3010, 3227
VspI ATTAAT 1 cut(s) 2255
XbaI TCTAGA 1 cut(s) 2731
XceI RCATGY 6 cut(s) 302, 411, 1439, 1771, 2289, 3124
XhoI CTCGAG 2 cut(s) 1912, 2989
XmiI GTMKAC 3 cut(s) 112, 1000, 2545
XmnI GAANNNNTTC 1 cut(s) 206
XspI CTAG 7 cut(s) 108, 587, 1269, 1443, 2673, 2732, 3080
ZrmI AGTACT 1 cut(s) 1060
Zsp2I ATGCAT 1 cut(s) 1437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.