Rroxscaffold_5G00374520

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004840
Physical Location & Seq
Reverse (-)
55524748 .. 55537188
12441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00374520.1

Sequence Viewer

Length: 609 bp
ATGGGAGGGTGGCCTCCCGGCAAGATACCGGCCGACCTTCACCATGGAAAGGTAGCTCGGGTGTCGATTTACCGGATGCATATCGTAGGTGGGGCTAGCTATGTTGTGGACCCCACCTATGAGGTCGTTGCTTATGCTTGGCGGTATGTGACGACCACTGATAGCGTGGAACTAATCCTTCATCAAGCTAATACAAATTACGCCATGAGGTATGAAGAGCTTATGACGAAACCTGATTTCTTAATTGGAGGGTGTGCTGGACTATTAGAGAAGGTGTTGATTATTAGAGAAGGTGTTTCCAAGAAGAAGGAGAAGGATGCCAAATCTATCCCCATTAACCACCAACATAATGACCAGGGCAAGTTGGCTGAGGTGGTAGATAATGCAAAGCTGGAAGAAGATCAAGCTCGGGTGCATGATGACCCTATAGGGTATATAGATTTGACAACAAAGCAAGAAAAATCAAAGCTATATCGCGAGGTGTTCGGTCCAGCTAAATCACGAGGTGTCTCGACCTGCCACTATCGCTATCGGTCTCGCTTATCTATAAAACAAACGAAGGTCGAGAGGGAAGACGGGTATGTCGGCCTTCAACTCTCCGATGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

202

Amino Acids

22.79

Weight (kDa)

8.38

Isoelectric Point (pI)

24.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 581
Acc36I ACCTGC 1 cut(s) 524
AccII CGCG 1 cut(s) 477
AciI CCGC 1 cut(s) 142
AcoI YGGCCR 1 cut(s) 30
AdeI CACNNNGTG 1 cut(s) 506
AfiI CCNNNNNNNGG 1 cut(s) 49
AgsI TTSAA 1 cut(s) 593
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 8 cut(s) 56, 99, 188, 220, 391, 407, 469, 494
AluI AGCT 8 cut(s) 56, 99, 188, 220, 391, 407, 469, 494
Alw26I GTCTC 2 cut(s) 514, 540
Ama87I CYCGRG 2 cut(s) 57, 408
AoxI GGCC 3 cut(s) 11, 30, 586
AspS9I GGNCC 2 cut(s) 109, 488
AsuC2I CCSGG 1 cut(s) 18
AsuHPI GGTGA 1 cut(s) 32
AsuNHI GCTAGC 1 cut(s) 95
AvaI CYCGRG 2 cut(s) 57, 408
AvaII GGWCC 2 cut(s) 109, 488
BauI CACGAG 1 cut(s) 501
BbsI GAAGAC 1 cut(s) 579
BbvCI CCTCAGC 1 cut(s) 369
BciT130I CCWGG 1 cut(s) 356
BcnI CCSGG 1 cut(s) 18
BcoDI GTCTC 2 cut(s) 514, 540
BfaI CTAG 1 cut(s) 96
BfmI CTRYAG 1 cut(s) 426
BfuAI ACCTGC 1 cut(s) 524
Bme1390I CCNGG 2 cut(s) 18, 356
Bme18I GGWCC 2 cut(s) 109, 488
BmeT110I CYCGRG 2 cut(s) 57, 408
BmgT120I GGNCC 2 cut(s) 109, 488
BmiI GGNNCC 1 cut(s) 111
BmrFI CCNGG 2 cut(s) 18, 356
BmsI GCATC 3 cut(s) 66, 307, 592
BmtI GCTAGC 1 cut(s) 99
BpiI GAAGAC 1 cut(s) 579
Bpu10I CCTNAGC 1 cut(s) 369
BpuMI CCSGG 1 cut(s) 18
BsaBI GATNNNNATC 1 cut(s) 80
BsaI GGTCTC 1 cut(s) 540
BsaJI CCNNGG 2 cut(s) 43, 355
BsaWI WCCGGW 1 cut(s) 72
Bsc4I CCNNNNNNNGG 1 cut(s) 49
Bse118I RCCGGY 1 cut(s) 28
Bse8I GATNNNNATC 1 cut(s) 80
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 2 cut(s) 43, 355
BseGI GGATG 2 cut(s) 81, 322
BseJI GATNNNNATC 1 cut(s) 80
BseLI CCNNNNNNNGG 1 cut(s) 49
BseMII CTCAG 1 cut(s) 360
BseX3I CGGCCG 1 cut(s) 30
Bsh1236I CGCG 1 cut(s) 477
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 3 cut(s) 13, 32, 588
BsiEI CGRYCG 1 cut(s) 33
BsiHKCI CYCGRG 2 cut(s) 57, 408
BsiSI CCGG 3 cut(s) 18, 29, 73
BslI CCNNNNNNNGG 1 cut(s) 49
BsmAI GTCTC 2 cut(s) 514, 540
BsnI GGCC 3 cut(s) 13, 32, 588
Bso31I GGTCTC 1 cut(s) 540
BsoBI CYCGRG 2 cut(s) 57, 408
Bsp143I GATC 1 cut(s) 400
Bsp19I CCATGG 1 cut(s) 43
Bsp68I TCGCGA 1 cut(s) 477
BspACI CCGC 1 cut(s) 142
BspANI GGCC 3 cut(s) 13, 32, 588
BspCNI CTCAG 1 cut(s) 361
BspFNI CGCG 1 cut(s) 477
BspLI GGNNCC 1 cut(s) 111
BspMI ACCTGC 1 cut(s) 524
BspOI GCTAGC 1 cut(s) 99
BspQI GCTCTTC 1 cut(s) 210
BspTNI GGTCTC 1 cut(s) 540
BsrFI RCCGGY 1 cut(s) 28
BssAI RCCGGY 1 cut(s) 28
BssECI CCNNGG 2 cut(s) 43, 355
BssMI GATC 1 cut(s) 400
BssSI CACGAG 1 cut(s) 501
BssT1I CCWWGG 1 cut(s) 43
Bst2BI CACGAG 1 cut(s) 501
Bst2UI CCWGG 1 cut(s) 356
Bst6I CTCTTC 1 cut(s) 210
BstC8I GCNNGC 1 cut(s) 97
BstDEI CTNAG 1 cut(s) 369
BstDSI CCRYGG 1 cut(s) 43
BstF5I GGATG 2 cut(s) 81, 322
BstFNI CGCG 1 cut(s) 477
BstKTI GATC 1 cut(s) 403
BstMAI GTCTC 2 cut(s) 514, 540
BstMBI GATC 1 cut(s) 400
BstMCI CGRYCG 1 cut(s) 33
BstMWI GCNNNNNNNGC 1 cut(s) 525
BstNI CCWGG 1 cut(s) 356
BstSCI CCNGG 2 cut(s) 16, 354
BstSFI CTRYAG 1 cut(s) 426
BstUI CGCG 1 cut(s) 477
BstV2I GAAGAC 1 cut(s) 579
BstZI CGGCCG 1 cut(s) 30
BsuRI GGCC 3 cut(s) 13, 32, 588
BtgI CCRYGG 1 cut(s) 43
BtsCI GGATG 2 cut(s) 81, 322
BtsIMutI CAGTG 1 cut(s) 156
BtuMI TCGCGA 1 cut(s) 477
BveI ACCTGC 1 cut(s) 524
Cac8I GCNNGC 1 cut(s) 97
Cfr10I RCCGGY 1 cut(s) 28
Cfr13I GGNCC 2 cut(s) 109, 488
CviAII CATG 3 cut(s) 44, 205, 416
DdeI CTNAG 1 cut(s) 369
DpnI GATC 1 cut(s) 402
DpnII GATC 1 cut(s) 400
DraIII CACNNNGTG 1 cut(s) 506
DrdI GACNNNNNNGTC 1 cut(s) 581
DseDI GACNNNNNNGTC 1 cut(s) 581
EaeI YGGCCR 1 cut(s) 30
EagI CGGCCG 1 cut(s) 30
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
EclXI CGGCCG 1 cut(s) 30
Eco130I CCWWGG 1 cut(s) 43
Eco31I GGTCTC 1 cut(s) 540
Eco47I GGWCC 2 cut(s) 109, 488
Eco52I CGGCCG 1 cut(s) 30
Eco88I CYCGRG 2 cut(s) 57, 408
EcoRII CCWGG 1 cut(s) 354
EcoT14I CCWWGG 1 cut(s) 43
EcoT22I ATGCAT 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 43
FaeI CATG 3 cut(s) 47, 208, 419
FatI CATG 3 cut(s) 43, 204, 415
FokI GGATG 2 cut(s) 88, 329
FspBI CTAG 1 cut(s) 96
HaeIII GGCC 3 cut(s) 13, 32, 588
HapII CCGG 3 cut(s) 18, 29, 73
Hin1II CATG 3 cut(s) 47, 208, 419
HpaII CCGG 3 cut(s) 18, 29, 73
HphI GGTGA 1 cut(s) 32
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 1 cut(s) 601
Hpy188III TCNNGA 4 cut(s) 476, 501, 511, 565
Hpy8I GTNNAC 1 cut(s) 109
HpyAV CCTTC 8 cut(s) 47, 188, 265, 284, 301, 307, 553, 599
HpyCH4V TGCA 3 cut(s) 79, 386, 415
HpyF10VI GCNNNNNNNGC 1 cut(s) 525
HpyF3I CTNAG 1 cut(s) 369
Hsp92II CATG 3 cut(s) 47, 208, 419
Kzo9I GATC 1 cut(s) 400
LguI GCTCTTC 1 cut(s) 210
LweI GCATC 3 cut(s) 66, 307, 592
MaeI CTAG 1 cut(s) 96
MaeIII GTNAC 1 cut(s) 148
MalI GATC 1 cut(s) 402
MboI GATC 1 cut(s) 400
MboII GAAGA 5 cut(s) 227, 316, 407, 410, 584
MluCI AATT 2 cut(s) 196, 243
MnlI CCTC 8 cut(s) 24, 115, 201, 242, 364, 472, 497, 561
Mph1103I ATGCAT 1 cut(s) 81
MseI TTAA 2 cut(s) 242, 336
MspI CCGG 3 cut(s) 18, 29, 73
MspR9I CCNGG 2 cut(s) 18, 356
MvaI CCWGG 1 cut(s) 356
MvnI CGCG 1 cut(s) 477
MwoI GCNNNNNNNGC 1 cut(s) 525
NciI CCSGG 1 cut(s) 18
NcoI CCATGG 1 cut(s) 43
NdeII GATC 1 cut(s) 400
NheI GCTAGC 1 cut(s) 95
NlaIII CATG 3 cut(s) 47, 208, 419
NlaIV GGNNCC 1 cut(s) 111
NmuCI GTSAC 1 cut(s) 148
NruI TCGCGA 1 cut(s) 477
NsiI ATGCAT 1 cut(s) 81
PciSI GCTCTTC 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 354
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 111
PspPI GGNCC 2 cut(s) 109, 488
RruI TCGCGA 1 cut(s) 477
SapI GCTCTTC 1 cut(s) 210
SaqAI TTAA 2 cut(s) 242, 336
Sau3AI GATC 1 cut(s) 400
Sau96I GGNCC 2 cut(s) 109, 488
ScrFI CCNGG 2 cut(s) 18, 356
SfaNI GCATC 3 cut(s) 66, 307, 592
SfcI CTRYAG 1 cut(s) 426
SinI GGWCC 2 cut(s) 109, 488
Sse9I AATT 2 cut(s) 196, 243
SsiI CCGC 1 cut(s) 142
SspMI CTAG 1 cut(s) 96
StyD4I CCNGG 2 cut(s) 16, 354
StyI CCWWGG 1 cut(s) 43
TaqI TCGA 3 cut(s) 65, 512, 564
TaqII GACCGA 2 cut(s) 476, 522
TasI AATT 2 cut(s) 196, 243
Tru1I TTAA 2 cut(s) 242, 336
Tru9I TTAA 2 cut(s) 242, 336
TscAI CASTG 1 cut(s) 163
TseFI GTSAC 1 cut(s) 148
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 2 cut(s) 170, 228
TspRI CASTG 1 cut(s) 163
VpaK11BI GGWCC 2 cut(s) 109, 488
XcmI CCANNNNNNNNNTGG 1 cut(s) 163
XspI CTAG 1 cut(s) 96
Zsp2I ATGCAT 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.