Rh4DG074400

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004840
Physical Location & Seq
Reverse (-)
11064407 .. 11065552
1146 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG074400.1

Sequence Viewer

Length: 1146 bp
ATGGAGAAGTTTATGCGTCTCTGGGAGAACACCGAACCTGCAGCTGACTCAGCTTTTCCAAAGATGGAAACTCTTGAGGTGAAGAATTCTGGATTGACTAATCTAGAGTTGTCTGCAATATCCTTCCGGAATCTAACAATTTTAGAAGTAACCTTTTGCTGGCGATTGCAATATTTGACCACTTATTCGGTAGCTCGAAGTCTCGTGCACCTCAAAACTTTGAAAGTTGACGAATGTAAAAATATGAAAGAAATTTTCACAAGCGAGGGAATGGGAGAAGATGCAAGTGAATGTGAGATTGAGTTCAGCCAGTTGCAAAGTTTGGAACTTAGTGATTTAGTAAGTTTGGAACGGTTTTGCTCGAGCAATTGCAGAGTGAAGGTCCCAATCTTGGGAACTCTAAAGGTGAATTGGTGCCCGTTCAAATTGAAGATTTCCTCTGATAAGCTACTAGAAGTTGATGGTGTCCAAACTGAATCATGGTCGCAAAGCATGTCTATGCTGGAACAGGACAACAGACGTTTGCGGGAAGAATTAAGGGCCAAAGAAGGTAACAGCCTCCCGGAGACTCCAGTTGTTCTCGCGCTGCCAGAGACTGGTCCTTCTAGCTCGTTGACTGCCAAGAGAACTGCTGATCTTCCTGTGGAGGTGATAGCCCAGCTAAATGCTCTGCTTGGAATTGATCTGGCAACCCCTGCCTTGGACAAGAAGAGCTCTTCTAACCACATAGTGACTGAGCTAACTGCCCAACTTGAGGCAAGAAGGTCTGAGCTTATTGCCGGCAGCTCTCAAGTTGAGGCGGAATGTCAAGAAGTCATGGGCCGGGAGGCGGAACTTGCTAACATAAGACAACTAATCAAACTGCTTGAAGCTCGGGCCGCTACTCTTGCAGCAGCAGCCCCTCAAGTTGAAGATTCTCACTGCTCATTACATTCATGTTTAATGTTTTGTATTTTCCCAATATATATTCTCCGGTGCCCAAAAATTTTGTATATAACCACATTTCACTGTGGTAGTCTATGCGAGAGTGCCTTAACAATAAGTAAACCTTCAAAGTTGTTATTACTTGTTTTGACAAATTCCTCACTCCAATTTCCAGATTCTACTGCTCCACCTGGCCGCAATTGCTCGTCTTTTCTGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

381

Amino Acids

42.24

Weight (kDa)

5.24

Isoelectric Point (pI)

55.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 34 - 139 1.7e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 46
AccB1I GGYRCC 2 cut(s) 414, 975
AccB7I CCANNNNNTGG 1 cut(s) 596
AccII CGCG 1 cut(s) 584
AccIII TCCGGA 1 cut(s) 126
AciI CCGC 5 cut(s) 526, 800, 830, 879, 1120
AcoI YGGCCR 1 cut(s) 1117
AcsI RAATTY 4 cut(s) 85, 252, 984, 1078
AdeI CACNNNGTG 1 cut(s) 730
AfiI CCNNNNNNNGG 7 cut(s) 159, 391, 392, 596, 700, 754, 829
AgsI TTSAA 6 cut(s) 223, 424, 430, 869, 911, 1053
AjnI CCWGG 1 cut(s) 1114
Alw21I GWGCWC 2 cut(s) 210, 716
Alw26I GTCTC 4 cut(s) 23, 206, 560, 587
Alw44I GTGCAC 1 cut(s) 206
AlwNI CAGNNNCTG 1 cut(s) 596
Ama87I CYCGRG 2 cut(s) 361, 873
Aor13HI TCCGGA 1 cut(s) 126
AoxI GGCC 4 cut(s) 540, 820, 876, 1117
ApaLI GTGCAC 1 cut(s) 206
ApeKI GCWGC 6 cut(s) 41, 586, 783, 890, 893, 896
ApoI RAATTY 4 cut(s) 85, 252, 984, 1078
ArsI GACNNNNNNTTYG 2 cut(s) 169, 201
AspLEI GCGC 1 cut(s) 586
AspS9I GGNCC 5 cut(s) 382, 540, 599, 820, 876
AsuC2I CCSGG 2 cut(s) 563, 824
AsuHPI GGTGA 3 cut(s) 91, 418, 661
AvaI CYCGRG 2 cut(s) 361, 873
AvaII GGWCC 2 cut(s) 382, 599
BaeGI GKGCMC 3 cut(s) 210, 419, 980
BanI GGYRCC 2 cut(s) 414, 975
BanII GRGCYC 1 cut(s) 716
BauI CACGAG 1 cut(s) 203
Bbv12I GWGCWC 2 cut(s) 210, 716
BbvI GCAGC 6 cut(s) 53, 573, 795, 902, 905, 908
BccI CCATC 2 cut(s) 58, 455
BciT130I CCWGG 1 cut(s) 1116
BcnI CCSGG 2 cut(s) 563, 824
BcoDI GTCTC 4 cut(s) 23, 206, 560, 587
BfaI CTAG 3 cut(s) 104, 452, 606
BfmI CTRYAG 1 cut(s) 39
BfuAI ACCTGC 1 cut(s) 46
BisI GCNGC 8 cut(s) 42, 587, 784, 879, 891, 894, 897, 1120
BlsI GCNGC 8 cut(s) 43, 588, 785, 880, 892, 895, 898, 1121
Bme1390I CCNGG 3 cut(s) 563, 824, 1116
Bme18I GGWCC 2 cut(s) 382, 599
BmeT110I CYCGRG 2 cut(s) 361, 873
BmgT120I GGNCC 5 cut(s) 382, 540, 599, 820, 876
BmiI GGNNCC 3 cut(s) 384, 416, 977
BmrFI CCNGG 3 cut(s) 563, 824, 1116
BmsI GCATC 1 cut(s) 271
BpmI CTGGAG 1 cut(s) 555
BpuEI CTTGAG 4 cut(s) 95, 773, 774, 888
BpuMI CCSGG 2 cut(s) 563, 824
BsaJI CCNNGG 1 cut(s) 699
BsaWI WCCGGW 2 cut(s) 126, 972
Bsc4I CCNNNNNNNGG 7 cut(s) 159, 391, 392, 596, 700, 754, 829
Bse118I RCCGGY 1 cut(s) 779
Bse1I ACTGG 3 cut(s) 310, 572, 601
BseAI TCCGGA 1 cut(s) 126
BseBI CCWGG 1 cut(s) 1116
BseDI CCNNGG 1 cut(s) 699
BseLI CCNNNNNNNGG 7 cut(s) 159, 391, 392, 596, 700, 754, 829
BseMII CTCAG 3 cut(s) 63, 726, 759
BseNI ACTGG 3 cut(s) 310, 572, 601
BseSI GKGCMC 3 cut(s) 210, 419, 980
BseXI GCAGC 6 cut(s) 53, 573, 795, 902, 905, 908
BseYI CCCAGC 1 cut(s) 657
Bsh1236I CGCG 1 cut(s) 584
BshFI GGCC 4 cut(s) 542, 822, 878, 1119
BshNI GGYRCC 2 cut(s) 414, 975
BsiHKAI GWGCWC 2 cut(s) 210, 716
BsiHKCI CYCGRG 2 cut(s) 361, 873
BsiSI CCGG 5 cut(s) 127, 563, 780, 823, 973
BslFI GGGAC 1 cut(s) 368
BslI CCNNNNNNNGG 7 cut(s) 159, 391, 392, 596, 700, 754, 829
BsmAI GTCTC 4 cut(s) 23, 206, 560, 587
BsmBI CGTCTC 1 cut(s) 23
BsmFI GGGAC 1 cut(s) 368
BsnI GGCC 4 cut(s) 542, 822, 878, 1119
BsoBI CYCGRG 2 cut(s) 361, 873
Bsp1286I GDGCHC 4 cut(s) 210, 419, 716, 980
Bsp13I TCCGGA 1 cut(s) 126
Bsp143I GATC 2 cut(s) 634, 682
BspACI CCGC 5 cut(s) 526, 800, 830, 879, 1120
BspANI GGCC 4 cut(s) 542, 822, 878, 1119
BspCNI CTCAG 3 cut(s) 62, 727, 760
BspEI TCCGGA 1 cut(s) 126
BspFNI CGCG 1 cut(s) 584
BspLI GGNNCC 3 cut(s) 384, 416, 977
BspMAI CTGCAG 1 cut(s) 43
BspMI ACCTGC 1 cut(s) 46
BspQI GCTCTTC 2 cut(s) 704, 721
BspT107I GGYRCC 2 cut(s) 414, 975
BsrFI RCCGGY 1 cut(s) 779
BsrI ACTGG 3 cut(s) 310, 572, 601
BssAI RCCGGY 1 cut(s) 779
BssECI CCNNGG 1 cut(s) 699
BssMI GATC 2 cut(s) 634, 682
BssSI CACGAG 1 cut(s) 203
BssT1I CCWWGG 1 cut(s) 699
Bst2BI CACGAG 1 cut(s) 203
Bst2UI CCWGG 1 cut(s) 1116
Bst4CI ACNGT 2 cut(s) 354, 1010
Bst6I CTCTTC 2 cut(s) 704, 721
BstAPI GCANNNNNTGC 1 cut(s) 695
BstC8I GCNNGC 2 cut(s) 161, 781
BstDEI CTNAG 4 cut(s) 49, 329, 735, 768
BstFNI CGCG 1 cut(s) 584
BstHHI GCGC 1 cut(s) 586
BstKTI GATC 2 cut(s) 637, 685
BstMAI GTCTC 4 cut(s) 23, 206, 560, 587
BstMBI GATC 2 cut(s) 634, 682
BstMWI GCNNNNNNNGC 7 cut(s) 50, 695, 836, 878, 887, 896, 1125
BstNI CCWGG 1 cut(s) 1116
BstNSI RCATGY 1 cut(s) 496
BstSCI CCNGG 3 cut(s) 561, 822, 1114
BstSFI CTRYAG 1 cut(s) 39
BstSLI GKGCMC 3 cut(s) 210, 419, 980
BstUI CGCG 1 cut(s) 584
BstV1I GCAGC 6 cut(s) 53, 573, 795, 902, 905, 908
BsuRI GGCC 4 cut(s) 542, 822, 878, 1119
BtsI GCAGTG 1 cut(s) 919
BtsIMutI CAGTG 2 cut(s) 919, 1006
BveI ACCTGC 1 cut(s) 46
Cac8I GCNNGC 2 cut(s) 161, 781
CaiI CAGNNNCTG 1 cut(s) 596
CfoI GCGC 1 cut(s) 586
Cfr10I RCCGGY 1 cut(s) 779
Cfr13I GGNCC 5 cut(s) 382, 540, 599, 820, 876
CseI GACGC 1 cut(s) 5
CviAII CATG 4 cut(s) 480, 493, 817, 936
DdeI CTNAG 4 cut(s) 49, 329, 735, 768
DpnI GATC 2 cut(s) 636, 684
DpnII GATC 2 cut(s) 634, 682
DraIII CACNNNGTG 1 cut(s) 730
EaeI YGGCCR 1 cut(s) 1117
Eam1104I CTCTTC 2 cut(s) 704, 721
EarI CTCTTC 2 cut(s) 704, 721
EciI GGCGGA 2 cut(s) 815, 845
Ecl136II GAGCTC 1 cut(s) 714
Eco130I CCWWGG 1 cut(s) 699
Eco24I GRGCYC 1 cut(s) 716
Eco47I GGWCC 2 cut(s) 382, 599
Eco53kI GAGCTC 1 cut(s) 714
Eco88I CYCGRG 2 cut(s) 361, 873
EcoICRI GAGCTC 1 cut(s) 714
EcoO109I RGGNCCY 1 cut(s) 382
EcoRI GAATTC 1 cut(s) 85
EcoRII CCWGG 1 cut(s) 1114
EcoT14I CCWWGG 1 cut(s) 699
EcoT38I GRGCYC 1 cut(s) 716
ErhI CCWWGG 1 cut(s) 699
Esp3I CGTCTC 1 cut(s) 23
FaeI CATG 4 cut(s) 483, 496, 820, 939
FalI AAGNNNNNCTT 2 cut(s) 1033, 1065
FaqI GGGAC 1 cut(s) 368
FatI CATG 4 cut(s) 479, 492, 816, 935
FauI CCCGC 1 cut(s) 519
Fnu4HI GCNGC 8 cut(s) 42, 587, 784, 879, 891, 894, 897, 1120
FriOI GRGCYC 1 cut(s) 716
Fsp4HI GCNGC 8 cut(s) 42, 587, 784, 879, 891, 894, 897, 1120
FspBI CTAG 3 cut(s) 104, 452, 606
GlaI GCGC 1 cut(s) 585
GluI GCNGC 8 cut(s) 42, 587, 784, 879, 891, 894, 897, 1120
GsaI CCCAGC 1 cut(s) 661
GsuI CTGGAG 1 cut(s) 555
HaeIII GGCC 4 cut(s) 542, 822, 878, 1119
HapII CCGG 5 cut(s) 127, 563, 780, 823, 973
HgaI GACGC 1 cut(s) 5
HhaI GCGC 1 cut(s) 586
Hin1II CATG 4 cut(s) 483, 496, 820, 939
Hin6I GCGC 1 cut(s) 584
HinP1I GCGC 1 cut(s) 584
HincII GTYRAC 2 cut(s) 229, 615
HindII GTYRAC 2 cut(s) 229, 615
HinfI GANTC 6 cut(s) 47, 130, 476, 568, 914, 1100
HpaII CCGG 5 cut(s) 127, 563, 780, 823, 973
HphI GGTGA 3 cut(s) 91, 418, 661
Hpy166II GTNNAC 4 cut(s) 208, 229, 615, 1046
Hpy188I TCNGA 3 cut(s) 442, 769, 1145
Hpy188III TCNNGA 6 cut(s) 74, 90, 104, 127, 809, 1097
Hpy8I GTNNAC 4 cut(s) 208, 229, 615, 1046
HpyAV CCTTC 6 cut(s) 133, 373, 542, 612, 756, 1059
HpyCH4III ACNGT 2 cut(s) 354, 1010
HpyCH4IV ACGT 1 cut(s) 520
HpyCH4V TGCA 8 cut(s) 41, 116, 169, 208, 284, 316, 372, 890
HpyF10VI GCNNNNNNNGC 7 cut(s) 50, 695, 836, 878, 887, 896, 1125
HpyF3I CTNAG 4 cut(s) 49, 329, 735, 768
HpySE526I ACGT 1 cut(s) 520
Hsp92II CATG 4 cut(s) 483, 496, 820, 939
HspAI GCGC 1 cut(s) 584
Kpn2I TCCGGA 1 cut(s) 126
KroI GCCGGC 1 cut(s) 779
KroNI GCCGGC 1 cut(s) 781
Kzo9I GATC 2 cut(s) 634, 682
LguI GCTCTTC 2 cut(s) 704, 721
LmnI GCTCC 1 cut(s) 1114
Lsp1109I GCAGC 6 cut(s) 53, 573, 795, 902, 905, 908
LweI GCATC 1 cut(s) 271
MaeI CTAG 3 cut(s) 104, 452, 606
MaeII ACGT 1 cut(s) 520
MaeIII GTNAC 3 cut(s) 148, 551, 730
MalI GATC 2 cut(s) 636, 684
MboI GATC 2 cut(s) 634, 682
MboII GAAGA 8 cut(s) 94, 290, 442, 542, 629, 708, 721, 923
MfeI CAATTG 2 cut(s) 367, 1123
MhlI GDGCHC 4 cut(s) 210, 419, 716, 980
MlyI GAGTC 2 cut(s) 41, 562
MroI TCCGGA 1 cut(s) 126
MroNI GCCGGC 1 cut(s) 779
MseI TTAA 3 cut(s) 536, 941, 1034
MslI CAYNNNNRTG 1 cut(s) 497
MspA1I CMGCKG 1 cut(s) 44
MspI CCGG 5 cut(s) 127, 563, 780, 823, 973
MspR9I CCNGG 3 cut(s) 563, 824, 1116
MunI CAATTG 2 cut(s) 367, 1123
MvaI CCWGG 1 cut(s) 1116
MvnI CGCG 1 cut(s) 584
MwoI GCNNNNNNNGC 7 cut(s) 50, 695, 836, 878, 887, 896, 1125
NaeI GCCGGC 1 cut(s) 781
NciI CCSGG 2 cut(s) 563, 824
NdeII GATC 2 cut(s) 634, 682
NgoMIV GCCGGC 1 cut(s) 779
NlaIII CATG 4 cut(s) 483, 496, 820, 939
NlaIV GGNNCC 3 cut(s) 384, 416, 977
NmuCI GTSAC 1 cut(s) 730
NspI RCATGY 1 cut(s) 496
PaeR7I CTCGAG 1 cut(s) 361
PciSI GCTCTTC 2 cut(s) 704, 721
PdiI GCCGGC 1 cut(s) 781
PfeI GAWTC 4 cut(s) 130, 476, 914, 1100
PflMI CCANNNNNTGG 1 cut(s) 596
PfoI TCCNGGA 1 cut(s) 561
PkrI GCNGC 8 cut(s) 43, 588, 785, 880, 892, 895, 898, 1121
PleI GAGTC 2 cut(s) 41, 562
PpsI GAGTC 2 cut(s) 41, 562
PpuMI RGGWCCY 1 cut(s) 382
Psp124BI GAGCTC 1 cut(s) 716
Psp5II RGGWCCY 1 cut(s) 382
Psp6I CCWGG 1 cut(s) 1114
PspFI CCCAGC 1 cut(s) 657
PspGI CCWGG 1 cut(s) 1114
PspN4I GGNNCC 3 cut(s) 384, 416, 977
PspPI GGNCC 5 cut(s) 382, 540, 599, 820, 876
PspPPI RGGWCCY 1 cut(s) 382
PspXI VCTCGAGB 1 cut(s) 361
PstI CTGCAG 1 cut(s) 43
PstNI CAGNNNCTG 1 cut(s) 596
PvuII CAGCTG 1 cut(s) 44
RseI CAYNNNNRTG 1 cut(s) 497
SacI GAGCTC 1 cut(s) 716
SapI GCTCTTC 2 cut(s) 704, 721
SaqAI TTAA 3 cut(s) 536, 941, 1034
SatI GCNGC 8 cut(s) 42, 587, 784, 879, 891, 894, 897, 1120
Sau3AI GATC 2 cut(s) 634, 682
Sau96I GGNCC 5 cut(s) 382, 540, 599, 820, 876
SchI GAGTC 2 cut(s) 41, 562
ScrFI CCNGG 3 cut(s) 563, 824, 1116
SduI GDGCHC 4 cut(s) 210, 419, 716, 980
SfaNI GCATC 1 cut(s) 271
SfcI CTRYAG 1 cut(s) 39
Sfr274I CTCGAG 1 cut(s) 361
SinI GGWCC 2 cut(s) 382, 599
SlaI CTCGAG 1 cut(s) 361
SmiMI CAYNNNNRTG 1 cut(s) 497
SmlI CTYRAG 5 cut(s) 74, 361, 752, 789, 903
SmoI CTYRAG 5 cut(s) 74, 361, 752, 789, 903
SsiI CCGC 5 cut(s) 526, 800, 830, 879, 1120
SspI AATATT 1 cut(s) 173
SspMI CTAG 3 cut(s) 104, 452, 606
SstI GAGCTC 1 cut(s) 716
StyD4I CCNGG 3 cut(s) 561, 822, 1114
StyI CCWWGG 1 cut(s) 699
TaaI ACNGT 2 cut(s) 354, 1010
TaiI ACGT 1 cut(s) 523
TaqI TCGA 2 cut(s) 196, 362
TauI GCSGC 2 cut(s) 881, 1122
TfiI GAWTC 4 cut(s) 130, 476, 914, 1100
Tru1I TTAA 3 cut(s) 536, 941, 1034
Tru9I TTAA 3 cut(s) 536, 941, 1034
TscAI CASTG 2 cut(s) 926, 1013
TseFI GTSAC 1 cut(s) 730
TseI GCWGC 6 cut(s) 41, 586, 783, 890, 893, 896
Tsp45I GTSAC 1 cut(s) 730
TspDTI ATGAA 2 cut(s) 260, 924
TspRI CASTG 2 cut(s) 926, 1013
Van91I CCANNNNNTGG 1 cut(s) 596
VneI GTGCAC 1 cut(s) 206
VpaK11BI GGWCC 2 cut(s) 382, 599
XapI RAATTY 4 cut(s) 85, 252, 984, 1078
XbaI TCTAGA 1 cut(s) 103
XceI RCATGY 1 cut(s) 496
XhoI CTCGAG 1 cut(s) 361
XspI CTAG 3 cut(s) 104, 452, 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.