Rroxscaffold_5G00346710

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003962
Physical Location & Seq
Reverse (-)
17676134 .. 17677119
986 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00346710.1

Sequence Viewer

Length: 666 bp
ATGCCTCACCACTCTCATTTGGGGCCGGACGGACGCCTCGGTGCGGGTGGGAGGCACTTCCGCTCAAAATGCGCTGGCGGCAACAAGGCTCTGCGGCGCCTGCCCGAAATAGAGGGTGCCCGCCCGAGGCGCGATGGGAAGAGGGAGAGAGTGGCTCTTTCCGATGAGCCGGTGACTATTGCGGCGCGAGGGCCACCGATCGGCATGTTGACCCCCGCCACTTGGGTATCAAAGATTGGGTTCGCTACCCAACACCCTCTCGCTCGGCGCCGCTCCCCTCAACAAACAATACACCAGCCAAACGGAGCCATAATGAGCCCCACTCATGCTACCTCCAATGGTGCGGACTCCCGCCAAAGGAGTGACCTACGGGAGCACCGCCAAGCGGCTACCGCTGAGCCCCGGCTTGCCCCTAGATCGCCGGCCGAAGCGCCGCCACGCCTTGTGCCAAGACAGCATCGGGGGAGCTCCGGAGCGGGGACCGAAGCATATCGGTCCCACATCGAAAACATGAAGAGGATCAGCTCCTTCTTCACCTACAAAAGGCATCGAGAGAGAAGACCGCCCAACGCGGTCTCCCTCGACGCCCTTTGTATTTTACTTGACGGAGTAGCGGAAGCTATAAGGATAACACAAGTATCGGTCCGCTCGTCGGACCAGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

221

Amino Acids

24.33

Weight (kDa)

11.54

Isoelectric Point (pI)

74.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 96, 116, 267
AccBSI CCGCTC 4 cut(s) 63, 273, 476, 648
AccII CGCG 3 cut(s) 132, 187, 572
AccIII TCCGGA 1 cut(s) 470
AclWI GGATC 1 cut(s) 527
AcoI YGGCCR 1 cut(s) 423
AcyI GRCGYC 4 cut(s) 34, 97, 268, 585
AfiI CCNNNNNNNGG 9 cut(s) 43, 126, 200, 222, 357, 385, 477, 543, 652
AluBI AGCT 3 cut(s) 468, 525, 620
AluI AGCT 3 cut(s) 468, 525, 620
Alw21I GWGCWC 2 cut(s) 378, 470
Alw26I GTCTC 1 cut(s) 580
AlwI GGATC 1 cut(s) 527
Ama87I CYCGRG 1 cut(s) 124
Aor13HI TCCGGA 1 cut(s) 470
AoxI GGCC 3 cut(s) 23, 191, 423
AspLEI GCGC 6 cut(s) 74, 99, 132, 187, 270, 433
AspS9I GGNCC 6 cut(s) 23, 191, 480, 495, 643, 655
AsuC2I CCSGG 1 cut(s) 403
AsuHPI GGTGA 2 cut(s) 184, 526
AvaI CYCGRG 1 cut(s) 124
AvaII GGWCC 4 cut(s) 480, 495, 643, 655
BaeGI GKGCMC 1 cut(s) 121
BaeI ACNNNNGTAYC 4 cut(s) 210, 243, 621, 654
BanI GGYRCC 3 cut(s) 96, 116, 267
BanII GRGCYC 3 cut(s) 320, 402, 470
BbsI GAAGAC 1 cut(s) 565
Bbv12I GWGCWC 2 cut(s) 378, 470
BccI CCATC 1 cut(s) 128
BcnI CCSGG 1 cut(s) 403
BcoDI GTCTC 1 cut(s) 580
BfaI CTAG 1 cut(s) 414
BfoI RGCGCY 3 cut(s) 100, 271, 434
BisI GCNGC 6 cut(s) 79, 95, 183, 271, 387, 434
BlpI GCTNAGC 1 cut(s) 396
BlsI GCNGC 6 cut(s) 80, 96, 184, 272, 388, 435
Bme1390I CCNGG 1 cut(s) 403
Bme18I GGWCC 4 cut(s) 480, 495, 643, 655
BmeT110I CYCGRG 1 cut(s) 124
BmgT120I GGNCC 6 cut(s) 23, 191, 480, 495, 643, 655
BmiI GGNNCC 7 cut(s) 24, 98, 118, 269, 307, 481, 497
BmrFI CCNGG 1 cut(s) 403
BmsI GCATC 2 cut(s) 466, 556
BpiI GAAGAC 1 cut(s) 565
BplI GAGNNNNNCTC 4 cut(s) 139, 171, 307, 339
Bpu1102I GCTNAGC 1 cut(s) 396
BpuMI CCSGG 1 cut(s) 403
BsaHI GRCGYC 4 cut(s) 34, 97, 268, 585
BsaI GGTCTC 1 cut(s) 580
BsaJI CCNNGG 3 cut(s) 37, 125, 401
BsaWI WCCGGW 1 cut(s) 470
BsaXI ACNNNNNCTCC 2 cut(s) 560, 590
Bsc4I CCNNNNNNNGG 9 cut(s) 43, 126, 200, 222, 357, 385, 477, 543, 652
Bse118I RCCGGY 2 cut(s) 169, 421
BseAI TCCGGA 1 cut(s) 470
BseDI CCNNGG 3 cut(s) 37, 125, 401
BseLI CCNNNNNNNGG 9 cut(s) 43, 126, 200, 222, 357, 385, 477, 543, 652
BseMII CTCAG 1 cut(s) 387
BseSI GKGCMC 1 cut(s) 121
BseX3I CGGCCG 1 cut(s) 423
Bsh1236I CGCG 3 cut(s) 132, 187, 572
Bsh1285I CGRYCG 2 cut(s) 201, 426
BshFI GGCC 3 cut(s) 25, 193, 425
BshNI GGYRCC 3 cut(s) 96, 116, 267
BsiEI CGRYCG 2 cut(s) 201, 426
BsiHKAI GWGCWC 2 cut(s) 378, 470
BsiHKCI CYCGRG 1 cut(s) 124
BsiSI CCGG 5 cut(s) 26, 170, 403, 422, 471
BslFI GGGAC 2 cut(s) 481, 493
BslI CCNNNNNNNGG 9 cut(s) 43, 126, 200, 222, 357, 385, 477, 543, 652
BsmAI GTCTC 1 cut(s) 580
BsmFI GGGAC 2 cut(s) 481, 493
BsnI GGCC 3 cut(s) 25, 193, 425
Bso31I GGTCTC 1 cut(s) 580
BsoBI CYCGRG 1 cut(s) 124
Bsp1286I GDGCHC 5 cut(s) 121, 320, 378, 402, 470
Bsp13I TCCGGA 1 cut(s) 470
Bsp143I GATC 3 cut(s) 198, 416, 519
Bsp1720I GCTNAGC 1 cut(s) 396
BspANI GGCC 3 cut(s) 25, 193, 425
BspCNI CTCAG 1 cut(s) 388
BspEI TCCGGA 1 cut(s) 470
BspFNI CGCG 3 cut(s) 132, 187, 572
BspLI GGNNCC 7 cut(s) 24, 98, 118, 269, 307, 481, 497
BspPI GGATC 1 cut(s) 527
BspT107I GGYRCC 3 cut(s) 96, 116, 267
BspTNI GGTCTC 1 cut(s) 580
BsrBI CCGCTC 4 cut(s) 63, 273, 476, 648
BsrFI RCCGGY 2 cut(s) 169, 421
BssAI RCCGGY 2 cut(s) 169, 421
BssECI CCNNGG 3 cut(s) 37, 125, 401
BssMI GATC 3 cut(s) 198, 416, 519
BssNI GRCGYC 4 cut(s) 34, 97, 268, 585
Bst6I CTCTTC 2 cut(s) 134, 509
BstACI GRCGYC 4 cut(s) 34, 97, 268, 585
BstC8I GCNNGC 5 cut(s) 76, 101, 121, 408, 423
BstDEI CTNAG 1 cut(s) 396
BstENI CCTNNNNNAGG 1 cut(s) 541
BstFNI CGCG 3 cut(s) 132, 187, 572
BstH2I RGCGCY 3 cut(s) 100, 271, 434
BstHHI GCGC 6 cut(s) 74, 99, 132, 187, 270, 433
BstKTI GATC 3 cut(s) 201, 419, 522
BstMAI GTCTC 1 cut(s) 580
BstMBI GATC 3 cut(s) 198, 416, 519
BstMCI CGRYCG 2 cut(s) 201, 426
BstMWI GCNNNNNNNGC 6 cut(s) 69, 78, 100, 129, 392, 454
BstNSI RCATGY 1 cut(s) 208
BstSCI CCNGG 1 cut(s) 401
BstSLI GKGCMC 1 cut(s) 121
BstUI CGCG 3 cut(s) 132, 187, 572
BstV2I GAAGAC 1 cut(s) 565
BstZI CGGCCG 1 cut(s) 423
BsuRI GGCC 3 cut(s) 25, 193, 425
BtgZI GCGATG 1 cut(s) 147
Cac8I GCNNGC 5 cut(s) 76, 101, 121, 408, 423
CfoI GCGC 6 cut(s) 74, 99, 132, 187, 270, 433
Cfr10I RCCGGY 2 cut(s) 169, 421
Cfr13I GGNCC 6 cut(s) 23, 191, 480, 495, 643, 655
CpoI CGGWCCG 1 cut(s) 643
CseI GACGC 2 cut(s) 42, 593
CspI CGGWCCG 1 cut(s) 643
CviAII CATG 3 cut(s) 205, 326, 511
DdeI CTNAG 1 cut(s) 396
DinI GGCGCC 2 cut(s) 98, 269
DpnI GATC 3 cut(s) 200, 418, 521
DpnII GATC 3 cut(s) 198, 416, 519
EaeI YGGCCR 1 cut(s) 423
EagI CGGCCG 1 cut(s) 423
Eam1104I CTCTTC 2 cut(s) 134, 509
EarI CTCTTC 2 cut(s) 134, 509
Ecl136II GAGCTC 1 cut(s) 468
EclXI CGGCCG 1 cut(s) 423
Eco24I GRGCYC 3 cut(s) 320, 402, 470
Eco31I GGTCTC 1 cut(s) 580
Eco47I GGWCC 4 cut(s) 480, 495, 643, 655
Eco52I CGGCCG 1 cut(s) 423
Eco53kI GAGCTC 1 cut(s) 468
Eco88I CYCGRG 1 cut(s) 124
EcoICRI GAGCTC 1 cut(s) 468
EcoNI CCTNNNNNAGG 1 cut(s) 541
EcoT38I GRGCYC 3 cut(s) 320, 402, 470
EgeI GGCGCC 2 cut(s) 98, 269
EheI GGCGCC 2 cut(s) 98, 269
FaeI CATG 3 cut(s) 208, 329, 514
FaiI YATR 6 cut(s) 206, 311, 327, 490, 512, 623
FaqI GGGAC 2 cut(s) 481, 493
FatI CATG 3 cut(s) 204, 325, 510
FauI CCCGC 5 cut(s) 37, 128, 223, 359, 469
Fnu4HI GCNGC 6 cut(s) 79, 95, 183, 271, 387, 434
FriOI GRGCYC 3 cut(s) 320, 402, 470
Fsp4HI GCNGC 6 cut(s) 79, 95, 183, 271, 387, 434
FspBI CTAG 1 cut(s) 414
GlaI GCGC 6 cut(s) 73, 98, 131, 186, 269, 432
GluI GCNGC 6 cut(s) 79, 95, 183, 271, 387, 434
HaeII RGCGCY 3 cut(s) 100, 271, 434
HaeIII GGCC 3 cut(s) 25, 193, 425
HapII CCGG 5 cut(s) 26, 170, 403, 422, 471
HgaI GACGC 2 cut(s) 42, 593
HhaI GCGC 6 cut(s) 74, 99, 132, 187, 270, 433
Hin1I GRCGYC 4 cut(s) 34, 97, 268, 585
Hin1II CATG 3 cut(s) 208, 329, 514
Hin6I GCGC 6 cut(s) 72, 97, 130, 185, 268, 431
HinP1I GCGC 6 cut(s) 72, 97, 130, 185, 268, 431
HincII GTYRAC 1 cut(s) 210
HindII GTYRAC 1 cut(s) 210
HinfI GANTC 1 cut(s) 347
HpaII CCGG 5 cut(s) 26, 170, 403, 422, 471
HphI GGTGA 2 cut(s) 184, 526
Hpy166II GTNNAC 1 cut(s) 210
Hpy188I TCNGA 2 cut(s) 163, 655
Hpy188III TCNNGA 2 cut(s) 471, 551
Hpy8I GTNNAC 1 cut(s) 210
Hpy99I CGWCG 2 cut(s) 587, 655
HpyAV CCTTC 1 cut(s) 538
HpyF10VI GCNNNNNNNGC 6 cut(s) 69, 78, 100, 129, 392, 454
HpyF3I CTNAG 1 cut(s) 396
Hsp92I GRCGYC 4 cut(s) 34, 97, 268, 585
Hsp92II CATG 3 cut(s) 208, 329, 514
HspAI GCGC 6 cut(s) 72, 97, 130, 185, 268, 431
KasI GGCGCC 2 cut(s) 96, 267
Kpn2I TCCGGA 1 cut(s) 470
KroI GCCGGC 1 cut(s) 421
KroNI GCCGGC 1 cut(s) 423
Kzo9I GATC 3 cut(s) 198, 416, 519
LmnI GCTCC 7 cut(s) 278, 305, 373, 465, 473, 473, 530
LpnPI CCDG 8 cut(s) 39, 60, 113, 183, 308, 416, 435, 484
LweI GCATC 2 cut(s) 466, 556
MaeI CTAG 1 cut(s) 414
MaeIII GTNAC 2 cut(s) 172, 362
MalI GATC 3 cut(s) 200, 418, 521
MbiI CCGCTC 4 cut(s) 63, 273, 476, 648
MboI GATC 3 cut(s) 198, 416, 519
MboII GAAGA 4 cut(s) 151, 523, 526, 570
MhlI GDGCHC 5 cut(s) 121, 320, 378, 402, 470
Mly113I GGCGCC 2 cut(s) 97, 268
MlyI GAGTC 1 cut(s) 341
MmeI TCCRAC 1 cut(s) 633
MroI TCCGGA 1 cut(s) 470
MroNI GCCGGC 1 cut(s) 421
MseI TTAA 1 cut(s) 664
MspA1I CMGCKG 1 cut(s) 395
MspI CCGG 5 cut(s) 26, 170, 403, 422, 471
MspR9I CCNGG 1 cut(s) 403
MvnI CGCG 3 cut(s) 132, 187, 572
MwoI GCNNNNNNNGC 6 cut(s) 69, 78, 100, 129, 392, 454
NaeI GCCGGC 1 cut(s) 423
NarI GGCGCC 2 cut(s) 97, 268
NciI CCSGG 1 cut(s) 403
NdeII GATC 3 cut(s) 198, 416, 519
NgoMIV GCCGGC 1 cut(s) 421
NlaIII CATG 3 cut(s) 208, 329, 514
NlaIV GGNNCC 7 cut(s) 24, 98, 118, 269, 307, 481, 497
NmeAIII GCCGAG 1 cut(s) 244
NmuCI GTSAC 2 cut(s) 172, 362
NspI RCATGY 1 cut(s) 208
PcsI WCGNNNNNNNCGW 1 cut(s) 647
PdiI GCCGGC 1 cut(s) 423
PkrI GCNGC 6 cut(s) 80, 96, 184, 272, 388, 435
Ple19I CGATCG 1 cut(s) 201
PleI GAGTC 1 cut(s) 341
PluTI GGCGCC 2 cut(s) 100, 271
PpsI GAGTC 1 cut(s) 341
Psp124BI GAGCTC 1 cut(s) 470
PspN4I GGNNCC 7 cut(s) 24, 98, 118, 269, 307, 481, 497
PspPI GGNCC 6 cut(s) 23, 191, 480, 495, 643, 655
PvuI CGATCG 1 cut(s) 201
Rsr2I CGGWCCG 1 cut(s) 643
RsrII CGGWCCG 1 cut(s) 643
SacI GAGCTC 1 cut(s) 470
SaqAI TTAA 1 cut(s) 664
SatI GCNGC 6 cut(s) 79, 95, 183, 271, 387, 434
Sau3AI GATC 3 cut(s) 198, 416, 519
Sau96I GGNCC 6 cut(s) 23, 191, 480, 495, 643, 655
SchI GAGTC 1 cut(s) 341
ScrFI CCNGG 1 cut(s) 403
SduI GDGCHC 5 cut(s) 121, 320, 378, 402, 470
SetI ASST 6 cut(s) 335, 369, 470, 527, 539, 622
SfaNI GCATC 2 cut(s) 466, 556
SfoI GGCGCC 2 cut(s) 98, 269
SinI GGWCC 4 cut(s) 480, 495, 643, 655
SspDI GGCGCC 2 cut(s) 96, 267
SspMI CTAG 1 cut(s) 414
SstI GAGCTC 1 cut(s) 470
StyD4I CCNGG 1 cut(s) 401
TaqI TCGA 3 cut(s) 504, 550, 582
TaqII GACCGA 3 cut(s) 483, 497, 631
TauI GCSGC 6 cut(s) 81, 97, 185, 273, 389, 436
Tru1I TTAA 1 cut(s) 664
Tru9I TTAA 1 cut(s) 664
TseFI GTSAC 2 cut(s) 172, 362
Tsp45I GTSAC 2 cut(s) 172, 362
TspDTI ATGAA 1 cut(s) 527
TspGWI ACGGA 3 cut(s) 45, 318, 621
VpaK11BI GGWCC 4 cut(s) 480, 495, 643, 655
XagI CCTNNNNNAGG 1 cut(s) 541
XceI RCATGY 1 cut(s) 208
XspI CTAG 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.