Rorug04G0064400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003962
Physical Location & Seq
Reverse (-)
9988102 .. 9989369
1268 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0064400.1

Sequence Viewer

Length: 543 bp
ATGGCTCAGACGGTGGGAAGGACTGTAGCAGCTCCATTGCTGTTTATCAACTTGATCATGTACTTGATCGTAGTGGGATTTGCTAGTTGGTGCCTTAACAAGTTCATCAATGGCCAGACCAACCACCCAAGTTTTGGAGGAAATGGTGCAACAATGTTCTTTCTGATCTTCTCCATATTGGCTGGTGTGATGGGAATAGCGTCTAAGTTGGCTGGAGCAGGTCATCTCAGAGCTTGGAGGAGTGACAGTCTAGCTGCAGCCGGAGTTTCATCAATTGTGGCTTGGGCTCTCACTGTACTAGCCTTTGGATTGGCATGCAAGCAAATCAACATTGGAGGACACAGGGGATGGAGGCTGAAGGTTTTGGAGGCTTTTATAATAATCTTGACGTTCACCGAGCTTTTGTATCTCTTGCTACTTCATGCTGGTATCTGCAGCAGCAAGTATGGTCCGGGTTACCGCGACACCGACTACCCTGCTGCTCCCGGTGTCGATCCAATCCACAAGGGCGGAACTGGAGTCCCTGCTTCTAGGGTGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

180

Amino Acids

19.06

Weight (kDa)

9.67

Isoelectric Point (pI)

29.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AWPM-19 PF05512 15 - 143 6.7e-53 AWPM-19-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 377
Acc36I ACCTGC 1 cut(s) 209
AccB1I GGYRCC 1 cut(s) 90
AccB7I CCANNNNNTGG 1 cut(s) 134
AccII CGCG 1 cut(s) 462
AciI CCGC 2 cut(s) 460, 510
AclWI GGATC 1 cut(s) 488
AcoI YGGCCR 1 cut(s) 112
AcuI CTGAAG 1 cut(s) 377
AfaI GTAC 2 cut(s) 62, 297
AfiI CCNNNNNNNGG 1 cut(s) 134
AluBI AGCT 4 cut(s) 32, 233, 254, 400
AluI AGCT 4 cut(s) 32, 233, 254, 400
AlwI GGATC 1 cut(s) 488
AoxI GGCC 1 cut(s) 112
ApeKI GCWGC 6 cut(s) 29, 254, 257, 435, 438, 479
AspS9I GGNCC 1 cut(s) 449
AsuC2I CCSGG 2 cut(s) 453, 486
AsuHPI GGTGA 1 cut(s) 385
AvaII GGWCC 1 cut(s) 449
BalI TGGCCA 1 cut(s) 114
BanI GGYRCC 1 cut(s) 90
BanII GRGCYC 1 cut(s) 289
BbvI GCAGC 6 cut(s) 41, 241, 269, 447, 450, 466
BccI CCATC 2 cut(s) 184, 342
BcgI CGANNNNNNTGC 2 cut(s) 458, 492
BclI TGATCA 1 cut(s) 54
BcnI CCSGG 2 cut(s) 453, 486
BfaI CTAG 4 cut(s) 84, 251, 299, 531
BfmI CTRYAG 3 cut(s) 24, 255, 433
BfuAI ACCTGC 1 cut(s) 209
BisI GCNGC 6 cut(s) 30, 255, 258, 436, 439, 480
BlsI GCNGC 6 cut(s) 31, 256, 259, 437, 440, 481
Bme1390I CCNGG 2 cut(s) 453, 486
Bme18I GGWCC 1 cut(s) 449
BmgT120I GGNCC 1 cut(s) 449
BmiI GGNNCC 1 cut(s) 92
BmrFI CCNGG 2 cut(s) 453, 486
BpmI CTGGAG 2 cut(s) 234, 537
BpuMI CCSGG 2 cut(s) 453, 486
BsaXI ACNNNNNCTCC 2 cut(s) 232, 262
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse1I ACTGG 1 cut(s) 520
Bse3DI GCAATG 1 cut(s) 35
BseGI GGATG 1 cut(s) 353
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMI GCAATG 1 cut(s) 35
BseMII CTCAG 2 cut(s) 20, 241
BseNI ACTGG 1 cut(s) 520
BseRI GAGGAG 1 cut(s) 253
BseXI GCAGC 6 cut(s) 41, 241, 269, 447, 450, 466
Bsh1236I CGCG 1 cut(s) 462
BshFI GGCC 1 cut(s) 114
BshNI GGYRCC 1 cut(s) 90
BsiSI CCGG 3 cut(s) 261, 452, 486
BslFI GGGAC 1 cut(s) 506
BslI CCNNNNNNNGG 1 cut(s) 134
BsmFI GGGAC 1 cut(s) 506
BsnI GGCC 1 cut(s) 114
Bsp1286I GDGCHC 1 cut(s) 289
Bsp143I GATC 4 cut(s) 54, 66, 165, 493
BspACI CCGC 2 cut(s) 460, 510
BspANI GGCC 1 cut(s) 114
BspCNI CTCAG 2 cut(s) 19, 240
BspFNI CGCG 1 cut(s) 462
BspLI GGNNCC 1 cut(s) 92
BspMAI CTGCAG 2 cut(s) 259, 437
BspMI ACCTGC 1 cut(s) 209
BspPI GGATC 1 cut(s) 488
BspT107I GGYRCC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 35
BsrI ACTGG 1 cut(s) 520
BssMI GATC 4 cut(s) 54, 66, 165, 493
Bst4CI ACNGT 4 cut(s) 13, 25, 248, 295
BstC8I GCNNGC 2 cut(s) 316, 320
BstDEI CTNAG 3 cut(s) 6, 204, 227
BstEII GGTNACC 1 cut(s) 455
BstF5I GGATG 1 cut(s) 353
BstFNI CGCG 1 cut(s) 462
BstKTI GATC 4 cut(s) 57, 69, 168, 496
BstMBI GATC 4 cut(s) 54, 66, 165, 493
BstNSI RCATGY 1 cut(s) 318
BstPI GGTNACC 1 cut(s) 455
BstSCI CCNGG 2 cut(s) 451, 484
BstSFI CTRYAG 3 cut(s) 24, 255, 433
BstUI CGCG 1 cut(s) 462
BstV1I GCAGC 6 cut(s) 41, 241, 269, 447, 450, 466
BsuRI GGCC 1 cut(s) 114
BtsCI GGATG 1 cut(s) 353
BtsIMutI CAGTG 1 cut(s) 291
BveI ACCTGC 1 cut(s) 209
Cac8I GCNNGC 2 cut(s) 316, 320
Cfr13I GGNCC 1 cut(s) 449
CseI GACGC 1 cut(s) 189
Csp6I GTAC 2 cut(s) 61, 296
CviAII CATG 3 cut(s) 58, 315, 422
CviQI GTAC 2 cut(s) 61, 296
DdeI CTNAG 3 cut(s) 6, 204, 227
DpnI GATC 4 cut(s) 56, 68, 167, 495
DpnII GATC 4 cut(s) 54, 66, 165, 493
EaeI YGGCCR 1 cut(s) 112
EciI GGCGGA 1 cut(s) 525
Eco24I GRGCYC 1 cut(s) 289
Eco47I GGWCC 1 cut(s) 449
Eco57I CTGAAG 1 cut(s) 377
Eco91I GGTNACC 1 cut(s) 455
EcoO65I GGTNACC 1 cut(s) 455
EcoT38I GRGCYC 1 cut(s) 289
FaeI CATG 3 cut(s) 61, 318, 425
FaiI YATR 6 cut(s) 59, 176, 316, 377, 423, 447
FaqI GGGAC 1 cut(s) 506
FatI CATG 3 cut(s) 57, 314, 421
FbaI TGATCA 1 cut(s) 54
Fnu4HI GCNGC 6 cut(s) 30, 255, 258, 436, 439, 480
FokI GGATG 1 cut(s) 360
FriOI GRGCYC 1 cut(s) 289
Fsp4HI GCNGC 6 cut(s) 30, 255, 258, 436, 439, 480
FspBI CTAG 4 cut(s) 84, 251, 299, 531
GluI GCNGC 6 cut(s) 30, 255, 258, 436, 439, 480
GsuI CTGGAG 2 cut(s) 234, 537
HaeIII GGCC 1 cut(s) 114
HapII CCGG 3 cut(s) 261, 452, 486
HgaI GACGC 1 cut(s) 189
Hin1II CATG 3 cut(s) 61, 318, 425
HinfI GANTC 1 cut(s) 519
HpaII CCGG 3 cut(s) 261, 452, 486
HphI GGTGA 1 cut(s) 385
Hpy166II GTNNAC 1 cut(s) 393
Hpy188I TCNGA 3 cut(s) 9, 165, 230
Hpy188III TCNNGA 1 cut(s) 385
Hpy8I GTNNAC 1 cut(s) 393
HpyAV CCTTC 2 cut(s) 12, 352
HpyCH4III ACNGT 4 cut(s) 13, 25, 248, 295
HpyCH4IV ACGT 1 cut(s) 389
HpyCH4V TGCA 4 cut(s) 149, 257, 318, 435
HpyF3I CTNAG 3 cut(s) 6, 204, 227
HpySE526I ACGT 1 cut(s) 389
Hsp92II CATG 3 cut(s) 61, 318, 425
Ksp22I TGATCA 1 cut(s) 54
Kzo9I GATC 4 cut(s) 54, 66, 165, 493
LmnI GCTCC 3 cut(s) 37, 215, 487
Lsp1109I GCAGC 6 cut(s) 41, 241, 269, 447, 450, 466
MaeI CTAG 4 cut(s) 84, 251, 299, 531
MaeII ACGT 1 cut(s) 389
MaeIII GTNAC 2 cut(s) 242, 455
MalI GATC 4 cut(s) 56, 68, 167, 495
MboI GATC 4 cut(s) 54, 66, 165, 493
MboII GAAGA 1 cut(s) 160
MfeI CAATTG 1 cut(s) 273
MhlI GDGCHC 1 cut(s) 289
MlsI TGGCCA 1 cut(s) 114
MluCI AATT 1 cut(s) 273
MluNI TGGCCA 1 cut(s) 114
MlyI GAGTC 1 cut(s) 528
MnlI CCTC 5 cut(s) 131, 231, 329, 345, 361
Mox20I TGGCCA 1 cut(s) 114
MscI TGGCCA 1 cut(s) 114
MseI TTAA 1 cut(s) 96
Msp20I TGGCCA 1 cut(s) 114
MspI CCGG 3 cut(s) 261, 452, 486
MspR9I CCNGG 2 cut(s) 453, 486
MunI CAATTG 1 cut(s) 273
MvnI CGCG 1 cut(s) 462
NciI CCSGG 2 cut(s) 453, 486
NdeII GATC 4 cut(s) 54, 66, 165, 493
NlaIII CATG 3 cut(s) 61, 318, 425
NlaIV GGNNCC 1 cut(s) 92
NmuCI GTSAC 1 cut(s) 242
NspI RCATGY 1 cut(s) 318
PaeI GCATGC 1 cut(s) 318
PflMI CCANNNNNTGG 1 cut(s) 134
PkrI GCNGC 6 cut(s) 31, 256, 259, 437, 440, 481
PleI GAGTC 1 cut(s) 527
PpsI GAGTC 1 cut(s) 527
PsiI TTATAA 1 cut(s) 377
PspEI GGTNACC 1 cut(s) 455
PspN4I GGNNCC 1 cut(s) 92
PspPI GGNCC 1 cut(s) 449
PstI CTGCAG 2 cut(s) 259, 437
RsaI GTAC 2 cut(s) 62, 297
RsaNI GTAC 2 cut(s) 61, 296
SaqAI TTAA 1 cut(s) 96
SatI GCNGC 6 cut(s) 30, 255, 258, 436, 439, 480
Sau3AI GATC 4 cut(s) 54, 66, 165, 493
Sau96I GGNCC 1 cut(s) 449
SchI GAGTC 1 cut(s) 528
ScrFI CCNGG 2 cut(s) 453, 486
SduI GDGCHC 1 cut(s) 289
SetI ASST 7 cut(s) 34, 223, 235, 256, 363, 392, 402
SfcI CTRYAG 3 cut(s) 24, 255, 433
SinI GGWCC 1 cut(s) 449
SphI GCATGC 1 cut(s) 318
Sse9I AATT 1 cut(s) 273
SsiI CCGC 2 cut(s) 460, 510
SspMI CTAG 4 cut(s) 84, 251, 299, 531
StyD4I CCNGG 2 cut(s) 451, 484
TaaI ACNGT 4 cut(s) 13, 25, 248, 295
TaiI ACGT 1 cut(s) 392
TaqI TCGA 1 cut(s) 492
TasI AATT 1 cut(s) 273
TatI WGTACW 2 cut(s) 60, 295
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TscAI CASTG 1 cut(s) 298
TseFI GTSAC 1 cut(s) 242
TseI GCWGC 6 cut(s) 29, 254, 257, 435, 438, 479
Tsp45I GTSAC 1 cut(s) 242
TspDTI ATGAA 3 cut(s) 94, 258, 410
TspRI CASTG 1 cut(s) 298
Van91I CCANNNNNTGG 1 cut(s) 134
VpaK11BI GGWCC 1 cut(s) 449
XceI RCATGY 1 cut(s) 318
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
XspI CTAG 4 cut(s) 84, 251, 299, 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.