Rorug01G0211600

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0003962
Physical Location & Seq
Forward (+)
30004356 .. 30006017
1662 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0211600.1

Sequence Viewer

Length: 1662 bp
ATGGATGAGTATGCTTTGAATTCTGGCCAGGCTATAAATAAAGCGAAGTCTCATCTGTTCCTTGGGAAGCATGCTTTGTTGAGGCAACATAGAATTAGTCGGATTCTGGGGATTCATGTGGACAAACTTCCTTTCACTTATCTTGGAGTCCCAATTTTTACTGGCCATTCGAAACCTGAGCACCTTGTTTCAATTGCGGACAAAGTGCGATGTAAGCTAAACTCTTGGAAAAGTAGGCAATTATCCCAAGCAGGTAGAGTACAGCTCATTTCATCAGTGATTCATGGCATTTTGATATACAGTTTTCAAGTTTATGAATGGCCCAAGGCCCTGCTAAAGAAAGTTCAAAGATGGATCAGGAACTTCTTTTGGAACGGGGATCCTCTCAAGGATGGCTCTGCCCTTATCGCTTGGAACAAAGTCTGTACCCCAAAAGAGCAGGGGGGATTAGGCATAAAAAATATTTTTACTTTGAATAGAGCCTTGCTTCTCAAAAGATGTTGGGACGTTGCCTCTTGTGCGTCCACCTCTGCCAGTTTTTTGCATGATCGGTTTGTAAAAATTGGTCTGCAACCATACTCTTCTTATAAAAAATCTTTGGTTTGGCTAGGACTTAAGAAGCTCTGGCCAAGGTTGTTGGAAGGGGTTCAATGGCTAGTTGGTGATGGCAAATCTATTAGATTTTGGCGTGACAATTGGTTGGGGGAAAACGTCCTTGCTACTTCACTCAATTATGTTCAGGAGGTCCAAGAGTTATTGAATGATAAAGTTTCTTGCTTCATTGAGAATGGCCAGTGGAATTTACCGTCAAGCTTCTGTCGCACGTCACCTGCACTAGCCCAGTCCATCCGCTCAATTGAGCTACCAGAGGAGCCCACCCCAGATCAGGTAGTTTGGTCTGAAACGGCGTCAGGGTCATTGACATCCAGTGATGCTTACTGCGCCTTAGATCCCCCTACGCCTGGCCCTTCATGGTGCAAGCACATTTGGCATGGGGCAATACAGCCTAGAAAGAGTGTCACTACTTGGAAAGTCTTGCATGGAAAAATTCAAACAGATGATTTTCTTCAGAAACGGGGTACATGCCTATGTTCTAGATGCCTCCTCTGTGGAACAAACTCCGAATCACATATGCATCTTTTTCAGACTTGTGAGATCGCCAACTCATTATGGCATTGGATCCAACATCTCTTCAGATTGCGTTTTCCTCCAGGTACGTCGCTCAATGATTTTTTCCTTGATAATTTGCTATCCTACCTCGATTATTCCTCTAGGTTGCTATGGTACATAGCTGTCTACAACCTTCTTTGGTGCATCTGGCAAGAAAGGAACAAAATAAAGTTTGATGGGGGAGCTTTTTGTCTATCAAGATTTAAGCAGTTTTTCATACTTTCACTAAGGGAATCTGCAGGGTTGATCTTTGCCTCATCCTCACTAGCATCCACAGCGGCTCCCATTTTCGACCTATTGGGCATCTCCCCCTTACGACCCACGGCACCTCGTTTTATTCCAGTCACATGGACTCCCCCGCCAGAGAATTGGTTCAAAGCAAATTCAAATGGCTCTTTTCGTGATCCCAATCACGCTGGCTTTGGTGGCATTTTTAGAAATAGTGATGAAGCTTTCTTAGGTGCCTTCTCTTGTAAAGCTATAGTGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_3 zf-CCHC_4

Protein Analysis

553

Amino Acids

62.76

Weight (kDa)

9.38

Isoelectric Point (pI)

39.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 308 - 391 1.4e-19 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 588
AarI CACCTGC 1 cut(s) 838
Acc36I ACCTGC 2 cut(s) 242, 838
AccB1I GGYRCC 2 cut(s) 1495, 1631
AccBSI CCGCTC 1 cut(s) 852
AccI GTMKAC 1 cut(s) 1296
AciI CCGC 4 cut(s) 197, 850, 1448, 1529
AclWI GGATC 7 cut(s) 362, 374, 387, 944, 1174, 1187, 1568
AcoI YGGCCR 4 cut(s) 25, 163, 626, 790
AcsI RAATTY 4 cut(s) 19, 799, 1047, 1552
AcuI CTGAAG 2 cut(s) 1052, 1177
AcyI GRCGYC 1 cut(s) 908
AfaI GTAC 5 cut(s) 261, 427, 1081, 1216, 1286
AfiI CCNNNNNNNGG 2 cut(s) 886, 962
AflII CTTAAG 1 cut(s) 614
AjiI CACGTC 1 cut(s) 825
AjnI CCWGG 3 cut(s) 27, 961, 1210
AluBI AGCT 9 cut(s) 217, 265, 622, 813, 862, 1292, 1355, 1622, 1649
AluI AGCT 9 cut(s) 217, 265, 622, 813, 862, 1292, 1355, 1622, 1649
Alw21I GWGCWC 1 cut(s) 183
Alw26I GTCTC 1 cut(s) 54
AlwI GGATC 7 cut(s) 362, 374, 387, 944, 1174, 1187, 1568
AoxI GGCC 7 cut(s) 25, 163, 320, 327, 626, 790, 964
ApoI RAATTY 4 cut(s) 19, 799, 1047, 1552
Asp700I GAANNNNTTC 2 cut(s) 645, 1541
AspLEI GCGC 1 cut(s) 944
AspS9I GGNCC 4 cut(s) 321, 328, 745, 965
AsuHPI GGTGA 2 cut(s) 674, 819
AsuII TTCGAA 1 cut(s) 170
AvaII GGWCC 1 cut(s) 745
BalI TGGCCA 4 cut(s) 27, 165, 628, 792
BamHI GGATCC 2 cut(s) 379, 1179
BanI GGYRCC 2 cut(s) 1495, 1631
BanII GRGCYC 1 cut(s) 876
Bbv12I GWGCWC 1 cut(s) 183
BccI CCATC 5 cut(s) 345, 386, 659, 854, 1340
BceAI ACGGC 2 cut(s) 921, 1509
BciT130I CCWGG 3 cut(s) 29, 963, 1212
BcoDI GTCTC 1 cut(s) 54
BfaI CTAG 7 cut(s) 608, 656, 836, 1008, 1095, 1272, 1436
BfmI CTRYAG 2 cut(s) 1407, 1650
BfrI CTTAAG 1 cut(s) 614
BfuAI ACCTGC 2 cut(s) 242, 838
BisI GCNGC 1 cut(s) 1449
BlsI GCNGC 1 cut(s) 1450
Bme1390I CCNGG 3 cut(s) 29, 963, 1212
Bme18I GGWCC 1 cut(s) 745
BmgBI CACGTC 1 cut(s) 825
BmgT120I GGNCC 4 cut(s) 321, 328, 745, 965
BmiI GGNNCC 6 cut(s) 381, 873, 1181, 1452, 1497, 1633
BmrFI CCNGG 3 cut(s) 29, 963, 1212
BmrI ACTGGG 1 cut(s) 835
BmsI GCATC 6 cut(s) 922, 1088, 1144, 1323, 1448, 1482
BmuI ACTGGG 1 cut(s) 835
BplI GAGNNNNNCTC 2 cut(s) 249, 281
BpmI CTGGAG 1 cut(s) 1194
Bpu10I CCTNAGC 1 cut(s) 177
Bpu14I TTCGAA 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 371
BsaBI GATNNNNATC 1 cut(s) 1578
BsaHI GRCGYC 1 cut(s) 908
BsaJI CCNNGG 4 cut(s) 61, 324, 629, 1491
BsaXI ACNNNNNCTCC 4 cut(s) 1435, 1465, 1507, 1537
Bsc4I CCNNNNNNNGG 2 cut(s) 886, 962
Bse1I ACTGG 6 cut(s) 166, 534, 793, 841, 927, 1511
Bse8I GATNNNNATC 1 cut(s) 1578
BseBI CCWGG 3 cut(s) 29, 963, 1212
BseDI CCNNGG 4 cut(s) 61, 324, 629, 1491
BseGI GGATG 6 cut(s) 10, 397, 846, 923, 1427, 1439
BseJI GATNNNNATC 1 cut(s) 1578
BseLI CCNNNNNNNGG 2 cut(s) 886, 962
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 6 cut(s) 166, 534, 793, 841, 927, 1511
BseRI GAGGAG 2 cut(s) 884, 1094
BsgI GTGCAG 1 cut(s) 816
BshFI GGCC 7 cut(s) 27, 165, 322, 329, 628, 792, 966
BshNI GGYRCC 2 cut(s) 1495, 1631
BsiHKAI GWGCWC 1 cut(s) 183
BslFI GGGAC 2 cut(s) 134, 518
BslI CCNNNNNNNGG 2 cut(s) 886, 962
BsmAI GTCTC 1 cut(s) 54
BsmFI GGGAC 2 cut(s) 134, 518
BsnI GGCC 7 cut(s) 27, 165, 322, 329, 628, 792, 966
Bsp119I TTCGAA 1 cut(s) 170
Bsp1286I GDGCHC 2 cut(s) 183, 876
Bsp143I GATC 9 cut(s) 354, 379, 547, 883, 949, 1155, 1179, 1416, 1573
BspACI CCGC 4 cut(s) 197, 850, 1448, 1529
BspANI GGCC 7 cut(s) 27, 165, 322, 329, 628, 792, 966
BspCNI CTCAG 1 cut(s) 169
BspLI GGNNCC 6 cut(s) 381, 873, 1181, 1452, 1497, 1633
BspMAI CTGCAG 1 cut(s) 1411
BspMI ACCTGC 2 cut(s) 242, 838
BspPI GGATC 7 cut(s) 362, 374, 387, 944, 1174, 1187, 1568
BspT104I TTCGAA 1 cut(s) 170
BspT107I GGYRCC 2 cut(s) 1495, 1631
BspTI CTTAAG 1 cut(s) 614
BsrBI CCGCTC 1 cut(s) 852
BsrI ACTGG 6 cut(s) 166, 534, 793, 841, 927, 1511
BssECI CCNNGG 4 cut(s) 61, 324, 629, 1491
BssMI GATC 9 cut(s) 354, 379, 547, 883, 949, 1155, 1179, 1416, 1573
BssNI GRCGYC 1 cut(s) 908
BssT1I CCWWGG 3 cut(s) 61, 324, 629
Bst2UI CCWGG 3 cut(s) 29, 963, 1212
Bst4CI ACNGT 2 cut(s) 302, 807
Bst6I CTCTTC 2 cut(s) 586, 1196
BstACI GRCGYC 1 cut(s) 908
BstAFI CTTAAG 1 cut(s) 614
BstBI TTCGAA 1 cut(s) 170
BstC8I GCNNGC 3 cut(s) 72, 980, 1588
BstDEI CTNAG 4 cut(s) 177, 946, 1397, 1627
BstDSI CCRYGG 1 cut(s) 1491
BstF5I GGATG 6 cut(s) 10, 397, 846, 923, 1427, 1439
BstHHI GCGC 1 cut(s) 944
BstKTI GATC 9 cut(s) 357, 382, 550, 886, 952, 1158, 1182, 1419, 1576
BstMAI GTCTC 1 cut(s) 54
BstMBI GATC 9 cut(s) 354, 379, 547, 883, 949, 1155, 1179, 1416, 1573
BstMWI GCNNNNNNNGC 8 cut(s) 214, 407, 518, 819, 941, 988, 1445, 1596
BstNI CCWGG 3 cut(s) 29, 963, 1212
BstNSI RCATGY 2 cut(s) 74, 1086
BstSCI CCNGG 3 cut(s) 27, 961, 1210
BstSFI CTRYAG 2 cut(s) 1407, 1650
BstX2I RGATCY 3 cut(s) 379, 949, 1179
BstXI CCANNNNNNTGG 2 cut(s) 1518, 1539
BstYI RGATCY 3 cut(s) 379, 949, 1179
BsuRI GGCC 7 cut(s) 27, 165, 322, 329, 628, 792, 966
BtgI CCRYGG 1 cut(s) 1491
BtgZI GCGATG 1 cut(s) 223
BtrI CACGTC 1 cut(s) 825
BtsCI GGATG 6 cut(s) 10, 397, 846, 923, 1427, 1439
BtsIMutI CAGTG 3 cut(s) 282, 800, 934
BveI ACCTGC 2 cut(s) 242, 838
Cac8I GCNNGC 3 cut(s) 72, 980, 1588
CfoI GCGC 1 cut(s) 944
Cfr13I GGNCC 4 cut(s) 321, 328, 745, 965
CseI GACGC 2 cut(s) 510, 897
Csp6I GTAC 5 cut(s) 260, 426, 1080, 1215, 1285
CviAII CATG 9 cut(s) 71, 116, 284, 545, 972, 992, 1040, 1083, 1518
CviQI GTAC 5 cut(s) 260, 426, 1080, 1215, 1285
DdeI CTNAG 4 cut(s) 177, 946, 1397, 1627
DpnI GATC 9 cut(s) 356, 381, 549, 885, 951, 1157, 1181, 1418, 1575
DpnII GATC 9 cut(s) 354, 379, 547, 883, 949, 1155, 1179, 1416, 1573
EaeI YGGCCR 4 cut(s) 25, 163, 626, 790
Eam1104I CTCTTC 2 cut(s) 586, 1196
EarI CTCTTC 2 cut(s) 586, 1196
Eco130I CCWWGG 3 cut(s) 61, 324, 629
Eco24I GRGCYC 1 cut(s) 876
Eco47I GGWCC 1 cut(s) 745
Eco57I CTGAAG 2 cut(s) 1052, 1177
EcoO109I RGGNCCY 1 cut(s) 328
EcoRI GAATTC 1 cut(s) 19
EcoRII CCWGG 3 cut(s) 27, 961, 1210
EcoT14I CCWWGG 3 cut(s) 61, 324, 629
EcoT22I ATGCAT 1 cut(s) 1137
EcoT38I GRGCYC 1 cut(s) 876
ErhI CCWWGG 3 cut(s) 61, 324, 629
FaeI CATG 9 cut(s) 74, 119, 287, 548, 975, 995, 1043, 1086, 1521
FaqI GGGAC 2 cut(s) 134, 518
FatI CATG 9 cut(s) 70, 115, 283, 544, 971, 991, 1039, 1082, 1517
FauI CCCGC 1 cut(s) 1536
FauNDI CATATG 1 cut(s) 1131
FblI GTMKAC 1 cut(s) 1296
Fnu4HI GCNGC 1 cut(s) 1449
FokI GGATG 6 cut(s) 17, 404, 833, 910, 1414, 1426
FriOI GRGCYC 1 cut(s) 876
Fsp4HI GCNGC 1 cut(s) 1449
FspBI CTAG 7 cut(s) 608, 656, 836, 1008, 1095, 1272, 1436
GlaI GCGC 1 cut(s) 943
GluI GCNGC 1 cut(s) 1449
GsuI CTGGAG 1 cut(s) 1194
HaeIII GGCC 7 cut(s) 27, 165, 322, 329, 628, 792, 966
HgaI GACGC 2 cut(s) 510, 897
HhaI GCGC 1 cut(s) 944
Hin1I GRCGYC 1 cut(s) 908
Hin1II CATG 9 cut(s) 74, 119, 287, 548, 975, 995, 1043, 1086, 1521
Hin6I GCGC 1 cut(s) 942
HinP1I GCGC 1 cut(s) 942
HindIII AAGCTT 2 cut(s) 811, 1620
HinfI GANTC 7 cut(s) 103, 112, 147, 280, 1124, 1403, 1522
HphI GGTGA 2 cut(s) 674, 819
Hpy166II GTNNAC 3 cut(s) 121, 525, 1297
Hpy188I TCNGA 6 cut(s) 102, 901, 1071, 1123, 1146, 1196
Hpy188III TCNNGA 5 cut(s) 358, 740, 1095, 1368, 1571
Hpy8I GTNNAC 3 cut(s) 121, 525, 1297
Hpy99I CGWCG 1 cut(s) 1222
HpyAV CCTTC 4 cut(s) 635, 978, 1313, 1645
HpyCH4III ACNGT 2 cut(s) 302, 807
HpyCH4IV ACGT 4 cut(s) 507, 711, 824, 1217
HpyCH4V TGCA 8 cut(s) 544, 571, 833, 978, 1039, 1135, 1314, 1409
HpyF10VI GCNNNNNNNGC 8 cut(s) 214, 407, 518, 819, 941, 988, 1445, 1596
HpyF3I CTNAG 4 cut(s) 177, 946, 1397, 1627
HpySE526I ACGT 4 cut(s) 507, 711, 824, 1217
Hsp92I GRCGYC 1 cut(s) 908
Hsp92II CATG 9 cut(s) 74, 119, 287, 548, 975, 995, 1043, 1086, 1521
HspAI GCGC 1 cut(s) 942
Kzo9I GATC 9 cut(s) 354, 379, 547, 883, 949, 1155, 1179, 1416, 1573
LmnI GCTCC 3 cut(s) 871, 1352, 1456
LweI GCATC 6 cut(s) 922, 1088, 1144, 1323, 1448, 1482
MaeI CTAG 7 cut(s) 608, 656, 836, 1008, 1095, 1272, 1436
MaeII ACGT 4 cut(s) 507, 711, 824, 1217
MaeIII GTNAC 4 cut(s) 689, 825, 1018, 1513
MalI GATC 9 cut(s) 356, 381, 549, 885, 951, 1157, 1181, 1418, 1575
MbiI CCGCTC 1 cut(s) 852
MboI GATC 9 cut(s) 354, 379, 547, 883, 949, 1155, 1179, 1416, 1573
MboII GAAGA 3 cut(s) 573, 1058, 1183
MfeI CAATTG 3 cut(s) 192, 694, 855
MflI RGATCY 3 cut(s) 379, 949, 1179
MhlI GDGCHC 2 cut(s) 183, 876
MlsI TGGCCA 4 cut(s) 27, 165, 628, 792
MluNI TGGCCA 4 cut(s) 27, 165, 628, 792
MlyI GAGTC 2 cut(s) 156, 1516
MmeI TCCRAC 3 cut(s) 80, 618, 1207
Mox20I TGGCCA 4 cut(s) 27, 165, 628, 792
Mph1103I ATGCAT 1 cut(s) 1137
MroXI GAANNNNTTC 2 cut(s) 645, 1541
MscI TGGCCA 4 cut(s) 27, 165, 628, 792
MseI TTAA 2 cut(s) 615, 1374
MslI CAYNNNNRTG 1 cut(s) 1087
Msp20I TGGCCA 4 cut(s) 27, 165, 628, 792
MspA1I CMGCKG 1 cut(s) 1448
MspCI CTTAAG 1 cut(s) 614
MspR9I CCNGG 3 cut(s) 29, 963, 1212
MunI CAATTG 3 cut(s) 192, 694, 855
MvaI CCWGG 3 cut(s) 29, 963, 1212
MwoI GCNNNNNNNGC 8 cut(s) 214, 407, 518, 819, 941, 988, 1445, 1596
NdeI CATATG 1 cut(s) 1131
NdeII GATC 9 cut(s) 354, 379, 547, 883, 949, 1155, 1179, 1416, 1573
NlaIII CATG 9 cut(s) 74, 119, 287, 548, 975, 995, 1043, 1086, 1521
NlaIV GGNNCC 6 cut(s) 381, 873, 1181, 1452, 1497, 1633
NmuCI GTSAC 4 cut(s) 689, 825, 1018, 1513
NsiI ATGCAT 1 cut(s) 1137
NspI RCATGY 2 cut(s) 74, 1086
NspV TTCGAA 1 cut(s) 170
PaeI GCATGC 1 cut(s) 74
PaqCI CACCTGC 1 cut(s) 838
PdmI GAANNNNTTC 2 cut(s) 645, 1541
PfeI GAWTC 5 cut(s) 103, 112, 280, 1124, 1403
PkrI GCNGC 1 cut(s) 1450
PleI GAGTC 2 cut(s) 155, 1516
PpsI GAGTC 2 cut(s) 155, 1516
PsiI TTATAA 1 cut(s) 588
Psp6I CCWGG 3 cut(s) 27, 961, 1210
PspGI CCWGG 3 cut(s) 27, 961, 1210
PspN4I GGNNCC 6 cut(s) 381, 873, 1181, 1452, 1497, 1633
PspPI GGNCC 4 cut(s) 321, 328, 745, 965
PstI CTGCAG 1 cut(s) 1411
PsuI RGATCY 3 cut(s) 379, 949, 1179
RsaI GTAC 5 cut(s) 261, 427, 1081, 1216, 1286
RsaNI GTAC 5 cut(s) 260, 426, 1080, 1215, 1285
RseI CAYNNNNRTG 1 cut(s) 1087
SaqAI TTAA 2 cut(s) 615, 1374
SatI GCNGC 1 cut(s) 1449
Sau3AI GATC 9 cut(s) 354, 379, 547, 883, 949, 1155, 1179, 1416, 1573
Sau96I GGNCC 4 cut(s) 321, 328, 745, 965
SchI GAGTC 2 cut(s) 156, 1516
ScrFI CCNGG 3 cut(s) 29, 963, 1212
SduI GDGCHC 2 cut(s) 183, 876
SfaNI GCATC 6 cut(s) 922, 1088, 1144, 1323, 1448, 1482
SfcI CTRYAG 2 cut(s) 1407, 1650
SfuI TTCGAA 1 cut(s) 170
SinI GGWCC 1 cut(s) 745
SmiMI CAYNNNNRTG 1 cut(s) 1087
SmlI CTYRAG 2 cut(s) 386, 614
SmoI CTYRAG 2 cut(s) 386, 614
SphI GCATGC 1 cut(s) 74
SsiI CCGC 4 cut(s) 197, 850, 1448, 1529
SspI AATATT 1 cut(s) 463
SspMI CTAG 7 cut(s) 608, 656, 836, 1008, 1095, 1272, 1436
StyD4I CCNGG 3 cut(s) 27, 961, 1210
StyI CCWWGG 3 cut(s) 61, 324, 629
TaaI ACNGT 2 cut(s) 302, 807
TaiI ACGT 4 cut(s) 510, 714, 827, 1220
TaqI TCGA 3 cut(s) 170, 1260, 1461
TatI WGTACW 1 cut(s) 259
TauI GCSGC 1 cut(s) 1451
TfiI GAWTC 5 cut(s) 103, 112, 280, 1124, 1403
Tru1I TTAA 2 cut(s) 615, 1374
Tru9I TTAA 2 cut(s) 615, 1374
TscAI CASTG 3 cut(s) 282, 800, 934
TseFI GTSAC 4 cut(s) 689, 825, 1018, 1513
Tsp45I GTSAC 4 cut(s) 689, 825, 1018, 1513
TspDTI ATGAA 8 cut(s) 104, 261, 272, 330, 769, 960, 1375, 1632
TspRI CASTG 3 cut(s) 282, 800, 934
Vha464I CTTAAG 1 cut(s) 614
VpaK11BI GGWCC 1 cut(s) 745
XapI RAATTY 4 cut(s) 19, 799, 1047, 1552
XbaI TCTAGA 1 cut(s) 1094
XceI RCATGY 2 cut(s) 74, 1086
XmiI GTMKAC 1 cut(s) 1296
XmnI GAANNNNTTC 2 cut(s) 645, 1541
XspI CTAG 7 cut(s) 608, 656, 836, 1008, 1095, 1272, 1436
Zsp2I ATGCAT 1 cut(s) 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.