Rorug03G0187300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002546
Physical Location & Seq
Reverse (-)
16284630 .. 16296004
11375 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0187300.1

Sequence Viewer

Length: 930 bp
ATGTCGGACCCAAAACCCAGACTAGTCTGCTGCATCGGCGACGTCCACGGCTTCCACACCAAGCTCCAAAACCTCTGGTCCAATCTCGAATCCTCCATCCCCTCTTCAGACTTCGCCACCGCCCTCGTCATCTTCCTCGGCGACTACTGCGACCGCGGCCCAGACACCAACAAAGTCCTCGACTTCCTCATTTCCCTCCCCTCCAGATACCCACGTCAGAAACACGTCTTCATCGCCGGAAACCACGAGTTCGCCTTCGCCGGCTTCTTGAGAGTCCTGCCTCCGCCGCCGGACGGGTCCGAGTTCCGGGAGGGGTGGAAGGATTTCGGGGACAATGAGGAGAGAGAAGGGTGGTTTAAGGGCGAGGGGTACGAGGGAATGCACTTGCAGGGGAGGAGATGGGCTGGAAGTATGAAGGGGTTTAATAAGGCTAAGGGGACTGAGTACCAGGGTTCCATTTATGATGCTGGGCCCACTTTTGAGTCCTATGGAGTGCCTCATGGCTCTGCCGATCTGGTGAAGGCAGTTCCTGATGATCATAAAAAGTTTCTTGCCGATATGGTTTGGGTGCATGAAGAGGATGATGTCTGTGTAGAGATTGAGGGTGAAACCAAACACTGTAAATTGATTGCTGTGCATGCCGGTTTGGAGAAAGATAAAGATGTCAAACAACAGTTGGAGTTTCTGAAAGCCAGAGATACTCGGGTACCTAAGATAGAGGCTCTTGCTGGCAGGAAAGATGTGTGGAATATACCAGAGGAACTGAAAAAGACTCCAACTGTTATTGTAAGTGGACATCATGGGAAACTTCATATTGAAGGCTTAAGGCTGATTATTGATGAAGGCGGTGGTATGGCAAAGAACCCGGTGGCTGCAATTTTACTCCCCTCTATGAGGATAGTCCGTGATACAGACGTTTTGGCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

309

Amino Acids

34.49

Weight (kDa)

5.91

Isoelectric Point (pI)

40.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 9 - 112 2e-12 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 45
Acc65I GGTACC 1 cut(s) 706
AccB1I GGYRCC 1 cut(s) 706
AccII CGCG 1 cut(s) 156
AciI CCGC 6 cut(s) 120, 154, 156, 284, 287, 846
AcuI CTGAAG 1 cut(s) 90
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 3 cut(s) 371, 446, 708
AfiI CCNNNNNNNGG 3 cut(s) 293, 306, 894
AflII CTTAAG 1 cut(s) 823
AflIII ACRYGT 1 cut(s) 223
AgsI TTSAA 1 cut(s) 818
AhlI ACTAGT 1 cut(s) 22
AjiI CACGTC 2 cut(s) 215, 226
AjnI CCWGG 1 cut(s) 447
AluBI AGCT 1 cut(s) 64
AluI AGCT 1 cut(s) 64
AlwNI CAGNNNCTG 1 cut(s) 530
Ama87I CYCGRG 1 cut(s) 702
AoxI GGCC 2 cut(s) 157, 470
ApaI GGGCCC 1 cut(s) 474
ApeKI GCWGC 2 cut(s) 30, 872
Asp700I GAANNNNTTC 1 cut(s) 323
Asp718I GGTACC 1 cut(s) 706
AspS9I GGNCC 6 cut(s) 7, 78, 158, 297, 470, 471
AsuC2I CCSGG 2 cut(s) 308, 866
AsuHPI GGTGA 2 cut(s) 529, 617
AvaI CYCGRG 1 cut(s) 702
AvaII GGWCC 3 cut(s) 7, 78, 297
BaeGI GKGCMC 1 cut(s) 474
BaeI ACNNNNGTAYC 7 cut(s) 199, 232, 690, 690, 723, 723, 900
BanI GGYRCC 1 cut(s) 706
BanII GRGCYC 1 cut(s) 474
BauI CACGAG 1 cut(s) 245
BbsI GAAGAC 1 cut(s) 220
BbvI GCAGC 2 cut(s) 17, 859
BccI CCATC 2 cut(s) 104, 393
BceAI ACGGC 1 cut(s) 64
BciT130I CCWGG 1 cut(s) 449
BclI TGATCA 1 cut(s) 535
BcnI CCSGG 2 cut(s) 308, 866
BcuI ACTAGT 1 cut(s) 22
BfaI CTAG 2 cut(s) 23, 928
BfrI CTTAAG 1 cut(s) 823
BisI GCNGC 4 cut(s) 31, 157, 287, 873
BlsI GCNGC 4 cut(s) 32, 158, 288, 874
Bme1390I CCNGG 3 cut(s) 308, 449, 866
Bme18I GGWCC 3 cut(s) 7, 78, 297
BmeT110I CYCGRG 1 cut(s) 702
BmgBI CACGTC 2 cut(s) 215, 226
BmgT120I GGNCC 6 cut(s) 7, 78, 158, 297, 470, 471
BmiI GGNNCC 5 cut(s) 9, 298, 454, 472, 708
BmrFI CCNGG 3 cut(s) 308, 449, 866
BmsI GCATC 2 cut(s) 42, 454
BpiI GAAGAC 1 cut(s) 220
BpmI CTGGAG 1 cut(s) 187
Bpu10I CCTNAGC 1 cut(s) 432
BpuEI CTTGAG 1 cut(s) 289
BpuMI CCSGG 2 cut(s) 308, 866
BsaHI GRCGYC 1 cut(s) 42
BsaJI CCNNGG 4 cut(s) 46, 136, 154, 448
Bsc4I CCNNNNNNNGG 3 cut(s) 293, 306, 894
Bse118I RCCGGY 2 cut(s) 260, 641
BseBI CCWGG 1 cut(s) 449
BseDI CCNNGG 4 cut(s) 46, 136, 154, 448
BseGI GGATG 2 cut(s) 96, 586
BseLI CCNNNNNNNGG 3 cut(s) 293, 306, 894
BseMII CTCAG 1 cut(s) 432
BseRI GAGGAG 2 cut(s) 353, 409
BseSI GKGCMC 1 cut(s) 474
BseXI GCAGC 2 cut(s) 17, 859
BseYI CCCAGC 1 cut(s) 467
Bsh1236I CGCG 1 cut(s) 156
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 2 cut(s) 159, 472
BshNI GGYRCC 1 cut(s) 706
BsiEI CGRYCG 1 cut(s) 154
BsiHKCI CYCGRG 1 cut(s) 702
BsiSI CCGG 6 cut(s) 237, 261, 290, 307, 642, 866
BslFI GGGAC 2 cut(s) 344, 451
BslI CCNNNNNNNGG 3 cut(s) 293, 306, 894
BsmFI GGGAC 2 cut(s) 344, 451
BsmI GAATGC 1 cut(s) 384
BsnI GGCC 2 cut(s) 159, 472
BsoBI CYCGRG 1 cut(s) 702
Bsp120I GGGCCC 1 cut(s) 470
Bsp1286I GDGCHC 1 cut(s) 474
Bsp143I GATC 2 cut(s) 511, 535
BspACI CCGC 6 cut(s) 120, 154, 156, 284, 287, 846
BspANI GGCC 2 cut(s) 159, 472
BspCNI CTCAG 1 cut(s) 433
BspFNI CGCG 1 cut(s) 156
BspLI GGNNCC 5 cut(s) 9, 298, 454, 472, 708
BspT107I GGYRCC 1 cut(s) 706
BspTI CTTAAG 1 cut(s) 823
BsrFI RCCGGY 2 cut(s) 260, 641
BssAI RCCGGY 2 cut(s) 260, 641
BssECI CCNNGG 4 cut(s) 46, 136, 154, 448
BssMI GATC 2 cut(s) 511, 535
BssNI GRCGYC 1 cut(s) 42
BssSI CACGAG 1 cut(s) 245
Bst2BI CACGAG 1 cut(s) 245
Bst2UI CCWGG 1 cut(s) 449
Bst4CI ACNGT 3 cut(s) 620, 675, 781
Bst6I CTCTTC 2 cut(s) 109, 570
BstACI GRCGYC 1 cut(s) 42
BstAFI CTTAAG 1 cut(s) 823
BstC8I GCNNGC 3 cut(s) 262, 639, 730
BstDEI CTNAG 3 cut(s) 432, 441, 711
BstDSI CCRYGG 2 cut(s) 46, 154
BstENI CCTNNNNNAGG 1 cut(s) 892
BstF5I GGATG 2 cut(s) 96, 586
BstFNI CGCG 1 cut(s) 156
BstKTI GATC 2 cut(s) 514, 538
BstMBI GATC 2 cut(s) 511, 535
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 5 cut(s) 36, 147, 156, 286, 638
BstNI CCWGG 1 cut(s) 449
BstNSI RCATGY 1 cut(s) 641
BstSCI CCNGG 3 cut(s) 306, 447, 864
BstSLI GKGCMC 1 cut(s) 474
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 2 cut(s) 17, 859
BstV2I GAAGAC 1 cut(s) 220
BsuRI GGCC 2 cut(s) 159, 472
BtgI CCRYGG 2 cut(s) 46, 154
BtgZI GCGATG 1 cut(s) 217
BtrI CACGTC 2 cut(s) 215, 226
BtsCI GGATG 2 cut(s) 96, 586
BtsIMutI CAGTG 1 cut(s) 616
Cac8I GCNNGC 3 cut(s) 262, 639, 730
CaiI CAGNNNCTG 1 cut(s) 530
Cfr10I RCCGGY 2 cut(s) 260, 641
Cfr13I GGNCC 6 cut(s) 7, 78, 158, 297, 470, 471
Cfr42I CCGCGG 1 cut(s) 157
Csp6I GTAC 3 cut(s) 370, 445, 707
CviAII CATG 4 cut(s) 500, 572, 638, 800
CviQI GTAC 3 cut(s) 370, 445, 707
DdeI CTNAG 3 cut(s) 432, 441, 711
DpnI GATC 2 cut(s) 513, 537
DpnII GATC 2 cut(s) 511, 535
Eam1104I CTCTTC 2 cut(s) 109, 570
EarI CTCTTC 2 cut(s) 109, 570
EciI GGCGGA 1 cut(s) 273
Eco24I GRGCYC 1 cut(s) 474
Eco47I GGWCC 3 cut(s) 7, 78, 297
Eco57I CTGAAG 1 cut(s) 90
Eco88I CYCGRG 1 cut(s) 702
EcoNI CCTNNNNNAGG 1 cut(s) 892
EcoRII CCWGG 1 cut(s) 447
EcoT38I GRGCYC 1 cut(s) 474
FaeI CATG 4 cut(s) 503, 575, 641, 803
FaqI GGGAC 2 cut(s) 344, 451
FatI CATG 4 cut(s) 499, 571, 637, 799
FbaI TGATCA 1 cut(s) 535
Fnu4HI GCNGC 4 cut(s) 31, 157, 287, 873
FokI GGATG 2 cut(s) 83, 593
FriOI GRGCYC 1 cut(s) 474
Fsp4HI GCNGC 4 cut(s) 31, 157, 287, 873
FspBI CTAG 2 cut(s) 23, 928
GluI GCNGC 4 cut(s) 31, 157, 287, 873
GsaI CCCAGC 1 cut(s) 471
GsuI CTGGAG 1 cut(s) 187
HaeIII GGCC 2 cut(s) 159, 472
HapII CCGG 6 cut(s) 237, 261, 290, 307, 642, 866
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 4 cut(s) 503, 575, 641, 803
HinfI GANTC 4 cut(s) 89, 273, 482, 772
HpaII CCGG 6 cut(s) 237, 261, 290, 307, 642, 866
HphI GGTGA 2 cut(s) 529, 617
Hpy166II GTNNAC 2 cut(s) 46, 794
Hpy188I TCNGA 5 cut(s) 7, 109, 219, 301, 687
Hpy188III TCNNGA 4 cut(s) 86, 204, 268, 530
Hpy8I GTNNAC 2 cut(s) 46, 794
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 7 cut(s) 265, 313, 341, 409, 514, 812, 836
HpyCH4III ACNGT 3 cut(s) 620, 675, 781
HpyCH4IV ACGT 4 cut(s) 42, 214, 225, 915
HpyCH4V TGCA 6 cut(s) 33, 382, 388, 571, 637, 875
HpyF10VI GCNNNNNNNGC 5 cut(s) 36, 147, 156, 286, 638
HpyF3I CTNAG 3 cut(s) 432, 441, 711
HpySE526I ACGT 4 cut(s) 42, 214, 225, 915
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 4 cut(s) 503, 575, 641, 803
KpnI GGTACC 1 cut(s) 710
KroI GCCGGC 1 cut(s) 260
KroNI GCCGGC 1 cut(s) 262
Ksp22I TGATCA 1 cut(s) 535
KspI CCGCGG 1 cut(s) 157
Kzo9I GATC 2 cut(s) 511, 535
LmnI GCTCC 1 cut(s) 69
Lsp1109I GCAGC 2 cut(s) 17, 859
LweI GCATC 2 cut(s) 42, 454
MaeI CTAG 2 cut(s) 23, 928
MaeII ACGT 4 cut(s) 42, 214, 225, 915
MalI GATC 2 cut(s) 513, 537
MboI GATC 2 cut(s) 511, 535
MboII GAAGA 4 cut(s) 96, 124, 220, 587
MhlI GDGCHC 1 cut(s) 474
MluCI AATT 2 cut(s) 623, 876
MlyI GAGTC 3 cut(s) 282, 491, 766
MmeI TCCRAC 2 cut(s) 657, 800
MroNI GCCGGC 1 cut(s) 260
MroXI GAANNNNTTC 1 cut(s) 323
MseI TTAA 3 cut(s) 357, 423, 824
MspA1I CMGCKG 1 cut(s) 156
MspCI CTTAAG 1 cut(s) 823
MspI CCGG 6 cut(s) 237, 261, 290, 307, 642, 866
MspR9I CCNGG 3 cut(s) 308, 449, 866
Mva1269I GAATGC 1 cut(s) 384
MvaI CCWGG 1 cut(s) 449
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 5 cut(s) 36, 147, 156, 286, 638
NaeI GCCGGC 1 cut(s) 262
NciI CCSGG 2 cut(s) 308, 866
NdeII GATC 2 cut(s) 511, 535
NgoMIV GCCGGC 1 cut(s) 260
NlaIII CATG 4 cut(s) 503, 575, 641, 803
NlaIV GGNNCC 5 cut(s) 9, 298, 454, 472, 708
NmeAIII GCCGAG 1 cut(s) 117
NspI RCATGY 1 cut(s) 641
PaeI GCATGC 1 cut(s) 641
PctI GAATGC 1 cut(s) 384
PdiI GCCGGC 1 cut(s) 262
PdmI GAANNNNTTC 1 cut(s) 323
PfeI GAWTC 1 cut(s) 89
PfoI TCCNGGA 1 cut(s) 306
PkrI GCNGC 4 cut(s) 32, 158, 288, 874
PleI GAGTC 3 cut(s) 281, 490, 766
PpsI GAGTC 3 cut(s) 281, 490, 766
Psp6I CCWGG 1 cut(s) 447
PspFI CCCAGC 1 cut(s) 467
PspGI CCWGG 1 cut(s) 447
PspN4I GGNNCC 5 cut(s) 9, 298, 454, 472, 708
PspOMI GGGCCC 1 cut(s) 470
PspPI GGNCC 6 cut(s) 7, 78, 158, 297, 470, 471
PstNI CAGNNNCTG 1 cut(s) 530
RsaI GTAC 3 cut(s) 371, 446, 708
RsaNI GTAC 3 cut(s) 370, 445, 707
SacII CCGCGG 1 cut(s) 157
SaqAI TTAA 3 cut(s) 357, 423, 824
SatI GCNGC 4 cut(s) 31, 157, 287, 873
Sau3AI GATC 2 cut(s) 511, 535
Sau96I GGNCC 6 cut(s) 7, 78, 158, 297, 470, 471
SchI GAGTC 3 cut(s) 282, 491, 766
ScrFI CCNGG 3 cut(s) 308, 449, 866
SduI GDGCHC 1 cut(s) 474
SetI ASST 7 cut(s) 45, 66, 75, 217, 228, 712, 918
SfaNI GCATC 2 cut(s) 42, 454
Sfr303I CCGCGG 1 cut(s) 157
SgrBI CCGCGG 1 cut(s) 157
SinI GGWCC 3 cut(s) 7, 78, 297
SmlI CTYRAG 2 cut(s) 268, 823
SmoI CTYRAG 2 cut(s) 268, 823
SpeI ACTAGT 1 cut(s) 22
SphI GCATGC 1 cut(s) 641
Sse9I AATT 2 cut(s) 623, 876
SsiI CCGC 6 cut(s) 120, 154, 156, 284, 287, 846
SspMI CTAG 2 cut(s) 23, 928
StyD4I CCNGG 3 cut(s) 306, 447, 864
TaaI ACNGT 3 cut(s) 620, 675, 781
TaiI ACGT 4 cut(s) 45, 217, 228, 918
TaqI TCGA 2 cut(s) 87, 180
TasI AATT 2 cut(s) 623, 876
TauI GCSGC 2 cut(s) 159, 289
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 3 cut(s) 357, 423, 824
Tru9I TTAA 3 cut(s) 357, 423, 824
TscAI CASTG 1 cut(s) 623
TseI GCWGC 2 cut(s) 30, 872
TspDTI ATGAA 5 cut(s) 220, 428, 588, 800, 855
TspGWI ACGGA 1 cut(s) 893
TspRI CASTG 1 cut(s) 623
Vha464I CTTAAG 1 cut(s) 823
VpaK11BI GGWCC 3 cut(s) 7, 78, 297
XagI CCTNNNNNAGG 1 cut(s) 892
XceI RCATGY 1 cut(s) 641
XmnI GAANNNNTTC 1 cut(s) 323
XspI CTAG 2 cut(s) 23, 928
ZraI GACGTC 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.