Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0002546

Physical Location & Seq

Genomic Coordinates Forward (+)

46866016 .. 46888574

Gene Structure 22559 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG343700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1449 bp

ATGGGTATCAGCCTATCAGGGGAAGAAGGTAACACTTGCAGTCCACTTCTTTGTTTACCACCTGGAATCCTGGATATAGCTCACCGCAACAAAGACTACTGGTTTATGGACTTTCTTGGGCTCTTGTGGTATCTGTCCTTTCTCATCTTTAAGATGGTTTCTATGCAGAGACGAAGATTTTACACCTACTTTCAGCTCGAGTTCAGGATATTGAAGTTGCTTCTTTTTCTTGGCTACATGTCAATCATGAGCGTCTTGTTTGTATTTTGTGATCTCACTATTTCAGATCTATTTGCTGCTATGCTTGCTTTCTTTCCCACAGGGTGGGCCCCTCTTCAGATAGGACAAGTATGCAGCGTGATGTTCAGGAGCCAAGGATTCTCAGAATCGATAAATGAGTTTGGAAGAGCATACGATTATATAACGGGAGTGATAATCTTCATGCCCATTGCCATTTGTCATGGTTCCTATTTGTATCAAAATGCCAAACACGCTTGCTCTTTAACCAAGCATCAAGTAGAGGCCTATAAAGAGAAGGCAATTGGTGCATTATCAGATCAAATTAGCTTGTCAAATTTCAGAAATAAGACGGGCTTTGCTATGACAGTATCCAATTATCGCGAAGTGTTAAGGAACGCGGACAATAAGAAATGGCAAGCTAGATTAGGGAAAGGCATATACATCAATAGCATTTACATGGAAACATTTGATGCTGAAGAGGAAGCTGCCAGAACTTCTGATATTGCAATGATAAGGGTGAGAGGTAGGAGTGCGTTCCCCAATTTTGATATAAGTCTATATGATGTCCAAGCCATTCTGGATAGCACAACCTTTCCAATAAAAGGTGCTTTGAAGATGTTAAAGCGGAATTCAGTTGATGATGTGCATCAAAAGAGAAGAAAGACTCGCAATGTCTCCAGCTCAACCCTTGGTGCCCCACCATCACTTCACCCAATAGATCAAACTCAAACAACAACAATCAATTCATGCCTTCAGTATCAATTTTTGATCCCTTCCCTCCAAGTTACTGTCCCTCATGGTTACCAAAACCCTACCTCTGAAGCAAACCGAAGTTTCAATCCTCAGTATGACTATGGTGGAAGTGAGCCAACAGCCCAGTTTCAACCTATCATACCCTTACTGATTCAAGAGTTTCCATCTCACCAGTACTCTAACGGCAGTTCCCAGCACCACCTTGATCAAACCCCTAGCTCTCAATTCCTTCACGACACTCAGAACCCTAATCTTGTTGCTAGGTTGAACAATCTAGCAAGTGAAAGCTGTATGGAAGAATTTTCCAGCAGTCAGGTTCATGACGCGAGTTTCAAGATGATGGGGACGAACGAGGGTGGTGCTCATAGTGATGGCAGCAATCAAGTAGTTGGAAATCTTGGTGATCCTGAGGTTGGGCTGGGCAATTCTGACTTGGATCCTGCTAGCTGGCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003843 GO:0006810 GO:0008150 GO:0008194 GO:0010232 GO:0010233 GO:0016740 GO:0016757 GO:0016758 GO:0032501 GO:0033036 GO:0033037 GO:0035251 GO:0046527 GO:0051179 GO:0051234 GO:0052545 GO:0080165

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

482

Amino Acids

54.15

Weight (kDa)

6.11

Isoelectric Point (pI)

45.54

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002546)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g23031
malus_domestica	MD17G1047700.v1.1
prunus_persica	Prupe.7G069100_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0480851 RchiOBHm_Chr3g0480891
rosa_laevigata	RLG00000023444 RLG00000023445 RLG00000023447 RLG00000034573
rosa_multiflora	Rmu_sc0004058.1_g000024 Rmu_sc0004838.1_g000011 Rmu_sc0006028.1_g000028 Rmu_sc0012984.1_g000002 Rmu_sc0012984.1_g000013
rosa_roxburghii	Rroxscaffold_1G00032960 Rroxscaffold_6G00400920 Rroxscaffold_6G00400960
rosa_rugosa	Rorug03G0186900 Rorug03G0187000 Rorug03G0187000 Rorug03G0187200 Rorug03G0187300 Rorug05G0241400
rosa_samantha	Rh3AG238500 Rh3AG238600 Rh3BG272000 Rh3BG272500 Rh3BG272700 Rh3BG272800 Rh3CG267800 Rh3CG268300 Rh3DG264100 Rh3DG264400 Rh5AG321600 Rh5BG331900 Rh5CG357200 Rh5DG343700
rosa_wichuraiana	Rw0G006160 Rw3G021350 Rw3G021360 Rw5G030350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	932
AccB7I	CCANNNNNTGG	1 cut(s)	324
AccII	CGCG	3 cut(s)	621, 638, 1319
AciI	CCGC	3 cut(s)	85, 638, 865
AclWI	GGATC	4 cut(s)	1003, 1391, 1424, 1437
AcsI	RAATTY	3 cut(s)	574, 868, 1292
AcuI	CTGAAG	4 cut(s)	320, 735, 977, 1080
AdeI	CACNNNGTG	1 cut(s)	324
AfaI	GTAC	1 cut(s)	1169
AfiI	CCNNNNNNNGG	4 cut(s)	19, 324, 842, 1406
AflIII	ACRYGT	1 cut(s)	237
AgsI	TTSAA	7 cut(s)	214, 853, 1078, 1124, 1148, 1261, 1327
AjnI	CCWGG	2 cut(s)	61, 69
AluBI	AGCT	9 cut(s)	80, 196, 567, 659, 725, 921, 1212, 1281, 1440
AluI	AGCT	9 cut(s)	80, 196, 567, 659, 725, 921, 1212, 1281, 1440
Alw21I	GWGCWC	1 cut(s)	1357
Alw26I	GTCTC	2 cut(s)	163, 919
AlwI	GGATC	4 cut(s)	1003, 1391, 1424, 1437
Ama87I	CYCGRG	1 cut(s)	197
AoxI	GGCC	2 cut(s)	327, 522
ApaI	GGGCCC	1 cut(s)	331
ApeKI	GCWGC	4 cut(s)	296, 354, 725, 1368
ApoI	RAATTY	3 cut(s)	574, 868, 1292
AspS9I	GGNCC	2 cut(s)	327, 328
AsuHPI	GGTGA	5 cut(s)	74, 769, 941, 1154, 1406
AsuNHI	GCTAGC	1 cut(s)	1436
AvaI	CYCGRG	1 cut(s)	197
AxyI	CCTNAGG	1 cut(s)	1401
BaeGI	GKGCMC	2 cut(s)	331, 937
BamHI	GGATCC	1 cut(s)	1429
BanI	GGYRCC	1 cut(s)	932
BanII	GRGCYC	2 cut(s)	123, 331
Bbv12I	GWGCWC	1 cut(s)	1357
BbvI	GCAGC	4 cut(s)	283, 366, 712, 1380
BccI	CCATC	5 cut(s)	148, 949, 1165, 1327, 1358
BceAI	ACGGC	1 cut(s)	1192
BcgI	CGANNNNNNTGC	2 cut(s)	1334, 1368
BciT130I	CCWGG	2 cut(s)	63, 71
BciVI	GTATCC	1 cut(s)	619
BclI	TGATCA	1 cut(s)	1198
BcoDI	GTCTC	2 cut(s)	163, 919
BfaI	CTAG	5 cut(s)	660, 1209, 1254, 1268, 1437
BfmI	CTRYAG	1 cut(s)	1445
BfuI	GTATCC	1 cut(s)	619
BglII	AGATCT	1 cut(s)	286
BisI	GCNGC	4 cut(s)	297, 355, 726, 1369
BlsI	GCNGC	4 cut(s)	298, 356, 727, 1370
BmcAI	AGTACT	1 cut(s)	1169
Bme1390I	CCNGG	2 cut(s)	63, 71
BmeT110I	CYCGRG	1 cut(s)	197
BmgT120I	GGNCC	2 cut(s)	327, 328
BmiI	GGNNCC	6 cut(s)	329, 330, 371, 466, 934, 1431
BmrFI	CCNGG	2 cut(s)	63, 71
BmrI	ACTGGG	1 cut(s)	1111
BmsI	GCATC	3 cut(s)	520, 700, 895
BmtI	GCTAGC	1 cut(s)	1440
BmuI	ACTGGG	1 cut(s)	1111
BpmI	CTGGAG	1 cut(s)	901
Bsa29I	ATCGAT	1 cut(s)	389
BsaBI	GATNNNNATC	1 cut(s)	885
BsaJI	CCNNGG	2 cut(s)	373, 928
Bsc4I	CCNNNNNNNGG	4 cut(s)	19, 324, 842, 1406
Bse1I	ACTGG	3 cut(s)	104, 1117, 1165
Bse21I	CCTNAGG	1 cut(s)	1401
Bse3DI	GCAATG	3 cut(s)	447, 753, 916
Bse8I	GATNNNNATC	1 cut(s)	885
BseBI	CCWGG	2 cut(s)	63, 71
BseCI	ATCGAT	1 cut(s)	389
BseDI	CCNNGG	2 cut(s)	373, 928
BseJI	GATNNNNATC	1 cut(s)	885
BseLI	CCNNNNNNNGG	4 cut(s)	19, 324, 842, 1406
BseMI	GCAATG	3 cut(s)	447, 753, 916
BseMII	CTCAG	4 cut(s)	396, 1097, 1247, 1392
BseNI	ACTGG	3 cut(s)	104, 1117, 1165
BseSI	GKGCMC	2 cut(s)	331, 937
BseXI	GCAGC	4 cut(s)	283, 366, 712, 1380
BseYI	CCCAGC	2 cut(s)	1185, 1411
Bsh1236I	CGCG	3 cut(s)	621, 638, 1319
BshFI	GGCC	2 cut(s)	329, 524
BshNI	GGYRCC	1 cut(s)	932
BshVI	ATCGAT	1 cut(s)	389
BsiHKAI	GWGCWC	1 cut(s)	1357
BsiHKCI	CYCGRG	1 cut(s)	197
BslFI	GGGAC	2 cut(s)	1016, 1351
BslI	CCNNNNNNNGG	4 cut(s)	19, 324, 842, 1406
BsmAI	GTCTC	2 cut(s)	163, 919
BsmBI	CGTCTC	1 cut(s)	163
BsmFI	GGGAC	2 cut(s)	1016, 1351
BsnI	GGCC	2 cut(s)	329, 524
BsoBI	CYCGRG	1 cut(s)	197
Bsp120I	GGGCCC	1 cut(s)	327
Bsp1286I	GDGCHC	4 cut(s)	123, 331, 937, 1357
Bsp143I	GATC	8 cut(s)	271, 286, 556, 958, 1008, 1198, 1396, 1429
Bsp68I	TCGCGA	1 cut(s)	621
BspACI	CCGC	3 cut(s)	85, 638, 865
BspANI	GGCC	2 cut(s)	329, 524
BspCNI	CTCAG	4 cut(s)	395, 1096, 1246, 1393
BspDI	ATCGAT	1 cut(s)	389
BspFNI	CGCG	3 cut(s)	621, 638, 1319
BspHI	TCATGA	2 cut(s)	246, 1312
BspLI	GGNNCC	6 cut(s)	329, 330, 371, 466, 934, 1431
BspOI	GCTAGC	1 cut(s)	1440
BspPI	GGATC	4 cut(s)	1003, 1391, 1424, 1437
BspQI	GCTCTTC	1 cut(s)	400
BspT107I	GGYRCC	1 cut(s)	932
BsrDI	GCAATG	3 cut(s)	447, 753, 916
BsrI	ACTGG	3 cut(s)	104, 1117, 1165
BssECI	CCNNGG	2 cut(s)	373, 928
BssMI	GATC	8 cut(s)	271, 286, 556, 958, 1008, 1198, 1396, 1429
BssT1I	CCWWGG	2 cut(s)	373, 928
Bst2UI	CCWGG	2 cut(s)	63, 71
Bst4CI	ACNGT	2 cut(s)	607, 1030
Bst6I	CTCTTC	3 cut(s)	339, 400, 711
BstAPI	GCANNNNNTGC	1 cut(s)	545
BstC8I	GCNNGC	5 cut(s)	306, 496, 657, 1438, 1442
BstDEI	CTNAG	4 cut(s)	382, 1083, 1233, 1401
BstEII	GGTNACC	1 cut(s)	1040
BstFNI	CGCG	3 cut(s)	621, 638, 1319
BstKTI	GATC	8 cut(s)	274, 289, 559, 961, 1011, 1201, 1399, 1432
BstMAI	GTCTC	2 cut(s)	163, 919
BstMBI	GATC	8 cut(s)	271, 286, 556, 958, 1008, 1198, 1396, 1429
BstMWI	GCNNNNNNNGC	3 cut(s)	305, 491, 545
BstNI	CCWGG	2 cut(s)	63, 71
BstNSI	RCATGY	1 cut(s)	241
BstPI	GGTNACC	1 cut(s)	1040
BstSCI	CCNGG	2 cut(s)	61, 69
BstSFI	CTRYAG	1 cut(s)	1445
BstSLI	GKGCMC	2 cut(s)	331, 937
BstUI	CGCG	3 cut(s)	621, 638, 1319
BstV1I	GCAGC	4 cut(s)	283, 366, 712, 1380
BstX2I	RGATCY	2 cut(s)	286, 1429
BstYI	RGATCY	2 cut(s)	286, 1429
Bsu15I	ATCGAT	1 cut(s)	389
Bsu36I	CCTNAGG	1 cut(s)	1401
BsuI	GTATCC	1 cut(s)	619
BsuRI	GGCC	2 cut(s)	329, 524
BsuTUI	ATCGAT	1 cut(s)	389
BtuMI	TCGCGA	1 cut(s)	621
Cac8I	GCNNGC	5 cut(s)	306, 496, 657, 1438, 1442
CciI	TCATGA	2 cut(s)	246, 1312
Cfr13I	GGNCC	2 cut(s)	327, 328
ClaI	ATCGAT	1 cut(s)	389
CseI	GACGC	2 cut(s)	241, 1325
Csp6I	GTAC	1 cut(s)	1168
CviAII	CATG	8 cut(s)	238, 247, 442, 461, 697, 987, 1037, 1313
CviQI	GTAC	1 cut(s)	1168
DdeI	CTNAG	4 cut(s)	382, 1083, 1233, 1401
DpnI	GATC	8 cut(s)	273, 288, 558, 960, 1010, 1200, 1398, 1431
DpnII	GATC	8 cut(s)	271, 286, 556, 958, 1008, 1198, 1396, 1429
DraIII	CACNNNGTG	1 cut(s)	324
Eam1104I	CTCTTC	3 cut(s)	339, 400, 711
EarI	CTCTTC	3 cut(s)	339, 400, 711
Eco130I	CCWWGG	2 cut(s)	373, 928
Eco147I	AGGCCT	1 cut(s)	524
Eco24I	GRGCYC	2 cut(s)	123, 331
Eco57I	CTGAAG	4 cut(s)	320, 735, 977, 1080
Eco81I	CCTNAGG	1 cut(s)	1401
Eco88I	CYCGRG	1 cut(s)	197
Eco91I	GGTNACC	1 cut(s)	1040
EcoO109I	RGGNCCY	1 cut(s)	328
EcoO65I	GGTNACC	1 cut(s)	1040
EcoRI	GAATTC	1 cut(s)	868
EcoRII	CCWGG	2 cut(s)	61, 69
EcoT14I	CCWWGG	2 cut(s)	373, 928
EcoT38I	GRGCYC	2 cut(s)	123, 331
ErhI	CCWWGG	2 cut(s)	373, 928
Esp3I	CGTCTC	1 cut(s)	163
FaeI	CATG	8 cut(s)	241, 250, 445, 464, 700, 990, 1040, 1316
FalI	AAGNNNNNCTT	2 cut(s)	578, 610
FaqI	GGGAC	2 cut(s)	1016, 1351
FatI	CATG	8 cut(s)	237, 246, 441, 460, 696, 986, 1036, 1312
FbaI	TGATCA	1 cut(s)	1198
Fnu4HI	GCNGC	4 cut(s)	297, 355, 726, 1369
FriOI	GRGCYC	2 cut(s)	123, 331
Fsp4HI	GCNGC	4 cut(s)	297, 355, 726, 1369
FspBI	CTAG	5 cut(s)	660, 1209, 1254, 1268, 1437
GluI	GCNGC	4 cut(s)	297, 355, 726, 1369
GsaI	CCCAGC	2 cut(s)	1189, 1415
GsuI	CTGGAG	1 cut(s)	901
HaeIII	GGCC	2 cut(s)	329, 524
HgaI	GACGC	2 cut(s)	241, 1325
Hin1II	CATG	8 cut(s)	241, 250, 445, 464, 700, 990, 1040, 1316
HinfI	GANTC	5 cut(s)	66, 378, 386, 904, 1144
HphI	GGTGA	5 cut(s)	74, 769, 941, 1154, 1406
Hpy166II	GTNNAC	2 cut(s)	44, 56
Hpy188I	TCNGA	9 cut(s)	286, 339, 385, 556, 581, 739, 1060, 1236, 1423
Hpy8I	GTNNAC	2 cut(s)	44, 56
HpyAV	CCTTC	5 cut(s)	20, 529, 1001, 1023, 1232
HpyCH4III	ACNGT	2 cut(s)	607, 1030
HpyCH4V	TGCA	6 cut(s)	39, 166, 354, 548, 746, 886
HpyF10VI	GCNNNNNNNGC	3 cut(s)	305, 491, 545
HpyF3I	CTNAG	4 cut(s)	382, 1083, 1233, 1401
Hsp92II	CATG	8 cut(s)	241, 250, 445, 464, 700, 990, 1040, 1316
Ksp22I	TGATCA	1 cut(s)	1198
Kzo9I	GATC	8 cut(s)	271, 286, 556, 958, 1008, 1198, 1396, 1429
LguI	GCTCTTC	1 cut(s)	400
LmnI	GCTCC	1 cut(s)	369
Lsp1109I	GCAGC	4 cut(s)	283, 366, 712, 1380
LweI	GCATC	3 cut(s)	520, 700, 895
MaeI	CTAG	5 cut(s)	660, 1209, 1254, 1268, 1437
MaeIII	GTNAC	3 cut(s)	29, 1024, 1040
MalI	GATC	8 cut(s)	273, 288, 558, 960, 1010, 1200, 1398, 1431
MboI	GATC	8 cut(s)	271, 286, 556, 958, 1008, 1198, 1396, 1429
MboII	GAAGA	9 cut(s)	35, 186, 326, 417, 430, 728, 865, 909, 1301
MfeI	CAATTG	1 cut(s)	540
MflI	RGATCY	2 cut(s)	286, 1429
MhlI	GDGCHC	4 cut(s)	123, 331, 937, 1357
MlyI	GAGTC	1 cut(s)	898
MmeI	TCCRAC	1 cut(s)	1363
MseI	TTAA	4 cut(s)	150, 503, 629, 860
MslI	CAYNNNNRTG	2 cut(s)	695, 1362
MspR9I	CCNGG	2 cut(s)	63, 71
MunI	CAATTG	1 cut(s)	540
MvaI	CCWGG	2 cut(s)	63, 71
MvnI	CGCG	3 cut(s)	621, 638, 1319
MwoI	GCNNNNNNNGC	3 cut(s)	305, 491, 545
NdeII	GATC	8 cut(s)	271, 286, 556, 958, 1008, 1198, 1396, 1429
NheI	GCTAGC	1 cut(s)	1436
NlaIII	CATG	8 cut(s)	241, 250, 445, 464, 700, 990, 1040, 1316
NlaIV	GGNNCC	6 cut(s)	329, 330, 371, 466, 934, 1431
NruI	TCGCGA	1 cut(s)	621
NspI	RCATGY	1 cut(s)	241
PaeR7I	CTCGAG	1 cut(s)	197
PagI	TCATGA	2 cut(s)	246, 1312
PceI	AGGCCT	1 cut(s)	524
PciI	ACATGT	1 cut(s)	237
PciSI	GCTCTTC	1 cut(s)	400
PfeI	GAWTC	4 cut(s)	66, 378, 386, 1144
PflMI	CCANNNNNTGG	1 cut(s)	324
PfoI	TCCNGGA	1 cut(s)	69
PkrI	GCNGC	4 cut(s)	298, 356, 727, 1370
PleI	GAGTC	1 cut(s)	898
PpsI	GAGTC	1 cut(s)	898
PscI	ACATGT	1 cut(s)	237
Psp6I	CCWGG	2 cut(s)	61, 69
PspEI	GGTNACC	1 cut(s)	1040
PspFI	CCCAGC	2 cut(s)	1185, 1411
PspGI	CCWGG	2 cut(s)	61, 69
PspN4I	GGNNCC	6 cut(s)	329, 330, 371, 466, 934, 1431
PspOMI	GGGCCC	1 cut(s)	327
PspPI	GGNCC	2 cut(s)	327, 328
PspXI	VCTCGAGB	1 cut(s)	197
PsuI	RGATCY	2 cut(s)	286, 1429
RruI	TCGCGA	1 cut(s)	621
RsaI	GTAC	1 cut(s)	1169
RsaNI	GTAC	1 cut(s)	1168
RseI	CAYNNNNRTG	2 cut(s)	695, 1362
SapI	GCTCTTC	1 cut(s)	400
SaqAI	TTAA	4 cut(s)	150, 503, 629, 860
SatI	GCNGC	4 cut(s)	297, 355, 726, 1369
Sau3AI	GATC	8 cut(s)	271, 286, 556, 958, 1008, 1198, 1396, 1429
Sau96I	GGNCC	2 cut(s)	327, 328
ScaI	AGTACT	1 cut(s)	1169
SchI	GAGTC	1 cut(s)	898
ScrFI	CCNGG	2 cut(s)	63, 71
SduI	GDGCHC	4 cut(s)	123, 331, 937, 1357
SfaNI	GCATC	3 cut(s)	520, 700, 895
SfcI	CTRYAG	1 cut(s)	1445
Sfr274I	CTCGAG	1 cut(s)	197
SlaI	CTCGAG	1 cut(s)	197
SmiMI	CAYNNNNRTG	2 cut(s)	695, 1362
SmlI	CTYRAG	1 cut(s)	197
SmoI	CTYRAG	1 cut(s)	197
SseBI	AGGCCT	1 cut(s)	524
SsiI	CCGC	3 cut(s)	85, 638, 865
SspMI	CTAG	5 cut(s)	660, 1209, 1254, 1268, 1437
StuI	AGGCCT	1 cut(s)	524
StyD4I	CCNGG	2 cut(s)	61, 69
StyI	CCWWGG	2 cut(s)	373, 928
TaaI	ACNGT	2 cut(s)	607, 1030
TaqI	TCGA	2 cut(s)	198, 389
TatI	WGTACW	1 cut(s)	1167
TfiI	GAWTC	4 cut(s)	66, 378, 386, 1144
Tru1I	TTAA	4 cut(s)	150, 503, 629, 860
Tru9I	TTAA	4 cut(s)	150, 503, 629, 860
TseI	GCWGC	4 cut(s)	296, 354, 725, 1368
TspDTI	ATGAA	3 cut(s)	430, 975, 1301
Van91I	CCANNNNNTGG	1 cut(s)	324
XapI	RAATTY	3 cut(s)	574, 868, 1292
XceI	RCATGY	1 cut(s)	241
XhoI	CTCGAG	1 cut(s)	197
XspI	CTAG	5 cut(s)	660, 1209, 1254, 1268, 1437
ZrmI	AGTACT	1 cut(s)	1169

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.