Rh5CG146100

DNA recombination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0003943
Physical Location & Seq
Forward (+)
13140157 .. 13145081
4925 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG146100.1

Sequence Viewer

Length: 654 bp
ATGGGAAGGCTGCAGATGCCGGCGTTCGTAGATTTGGCGGCTGGTCAGGTGGCAATGGCGGCAGTGCTGGATCTCTGGTTGATGATTTTCTTCCCGAGCAAGACACTGGGAGTGTCCAGCTGGGCCTGCTGGGTTTTCATGGTGGATGCAGGCCTCAGGACCTTGATTGGGATTTGGGCCCAAAAGGCAATAAGAAGCAAGAATAGGCCTAAGTGTGCTAAAGATGAGCTGCTGACAAATGGTGGAACTGTTGGGGATGCTGGTGCATCATCCTCAGCAAGCAAACCAGTCTTAAATAGAACTATGAGTACATGCATCTTCATCAATCCAAACATTCCCGACAGAGCGGAGTACAAACAAAGTTTTTCTGAACTATTTCTTGCTCCAACAAAACAAATAATAGGAGCTGATGGACTGGAGGATGTAGAAAAAAAGACAGTCAAAGAACTCAACCTACTAGATCCAGAAACAGACAAGATATATGGCACCTGTCAGAGCGATAAACAGAATTATACACCTCAGGATGTTTGCCAAGATTTTGCACCATTGGCAAGGAATGATGGAAACAACAGCTTTCAAATTAAGACATTTGTAAATCCCATAGTAGCACCTGTCTACCTAATTGCAGCAAAAATTGCTCATTCGATGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
DUF223 Helitron_like_N PIF1 REPA_OB_2 Rep_fac-A_C

Protein Analysis

217

Amino Acids

23.77

Weight (kDa)

7.57

Isoelectric Point (pI)

36.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 485
AccBSI CCGCTC 1 cut(s) 347
AccI GTMKAC 1 cut(s) 615
AciI CCGC 3 cut(s) 38, 59, 347
AclWI GGATC 2 cut(s) 78, 455
AfaI GTAC 2 cut(s) 310, 353
AfiI CCNNNNNNNGG 1 cut(s) 168
AgsI TTSAA 1 cut(s) 578
AluBI AGCT 4 cut(s) 120, 229, 407, 573
AluI AGCT 4 cut(s) 120, 229, 407, 573
AlwI GGATC 2 cut(s) 78, 455
Ama87I CYCGRG 1 cut(s) 94
AoxI GGCC 4 cut(s) 123, 151, 177, 206
ApaI GGGCCC 1 cut(s) 181
ApeKI GCWGC 3 cut(s) 10, 229, 626
Asp700I GAANNNNTTC 1 cut(s) 375
AspS9I GGNCC 4 cut(s) 123, 159, 177, 178
AvaI CYCGRG 1 cut(s) 94
AvaII GGWCC 1 cut(s) 159
AxyI CCTNAGG 2 cut(s) 155, 519
BaeGI GKGCMC 1 cut(s) 181
BanI GGYRCC 1 cut(s) 485
BanII GRGCYC 1 cut(s) 181
BbvCI CCTCAGC 1 cut(s) 274
BbvI GCAGC 2 cut(s) 216, 638
BccI CCATC 2 cut(s) 404, 554
BfaI CTAG 1 cut(s) 458
BfmI CTRYAG 1 cut(s) 11
BglI GCCNNNNNGGC 1 cut(s) 185
BisI GCNGC 5 cut(s) 11, 39, 60, 230, 627
BlsI GCNGC 5 cut(s) 12, 40, 61, 231, 628
Bme18I GGWCC 1 cut(s) 159
BmeT110I CYCGRG 1 cut(s) 94
BmgT120I GGNCC 4 cut(s) 123, 159, 177, 178
BmiI GGNNCC 2 cut(s) 179, 487
BmrI ACTGGG 1 cut(s) 116
BmsI GCATC 5 cut(s) 6, 136, 247, 275, 324
BmuI ACTGGG 1 cut(s) 116
BpmI CTGGAG 1 cut(s) 437
Bpu10I CCTNAGC 1 cut(s) 274
Bsc4I CCNNNNNNNGG 1 cut(s) 168
Bse118I RCCGGY 1 cut(s) 19
Bse1I ACTGG 3 cut(s) 111, 287, 420
Bse21I CCTNAGG 2 cut(s) 155, 519
Bse3DI GCAATG 1 cut(s) 60
BseGI GGATG 5 cut(s) 151, 262, 269, 427, 529
BseLI CCNNNNNNNGG 1 cut(s) 168
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 3 cut(s) 169, 288, 533
BseNI ACTGG 3 cut(s) 111, 287, 420
BseSI GKGCMC 1 cut(s) 181
BseXI GCAGC 2 cut(s) 216, 638
BseYI CCCAGC 2 cut(s) 120, 129
BshFI GGCC 4 cut(s) 125, 153, 179, 208
BshNI GGYRCC 1 cut(s) 485
BsiHKCI CYCGRG 1 cut(s) 94
BsiSI CCGG 1 cut(s) 20
BslI CCNNNNNNNGG 1 cut(s) 168
BsnI GGCC 4 cut(s) 125, 153, 179, 208
BsoBI CYCGRG 1 cut(s) 94
Bsp120I GGGCCC 1 cut(s) 177
Bsp1286I GDGCHC 1 cut(s) 181
Bsp143I GATC 2 cut(s) 70, 460
BspACI CCGC 3 cut(s) 38, 59, 347
BspANI GGCC 4 cut(s) 125, 153, 179, 208
BspCNI CTCAG 3 cut(s) 168, 287, 532
BspLI GGNNCC 2 cut(s) 179, 487
BspMAI CTGCAG 1 cut(s) 15
BspPI GGATC 2 cut(s) 78, 455
BspT107I GGYRCC 1 cut(s) 485
BsrBI CCGCTC 1 cut(s) 347
BsrDI GCAATG 1 cut(s) 60
BsrFI RCCGGY 1 cut(s) 19
BsrI ACTGG 3 cut(s) 111, 287, 420
BssAI RCCGGY 1 cut(s) 19
BssMI GATC 2 cut(s) 70, 460
Bst4CI ACNGT 2 cut(s) 250, 439
BstAPI GCANNNNNTGC 1 cut(s) 635
BstC8I GCNNGC 4 cut(s) 21, 127, 151, 280
BstDEI CTNAG 4 cut(s) 155, 210, 274, 519
BstF5I GGATG 5 cut(s) 151, 262, 269, 427, 529
BstKTI GATC 2 cut(s) 73, 463
BstMBI GATC 2 cut(s) 70, 460
BstMWI GCNNNNNNNGC 6 cut(s) 16, 59, 126, 185, 548, 635
BstNSI RCATGY 1 cut(s) 315
BstSFI CTRYAG 1 cut(s) 11
BstSLI GKGCMC 1 cut(s) 181
BstV1I GCAGC 2 cut(s) 216, 638
BstX2I RGATCY 2 cut(s) 70, 460
BstYI RGATCY 2 cut(s) 70, 460
Bsu36I CCTNAGG 2 cut(s) 155, 519
BsuRI GGCC 4 cut(s) 125, 153, 179, 208
BtsCI GGATG 5 cut(s) 151, 262, 269, 427, 529
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 2 cut(s) 69, 104
Cac8I GCNNGC 4 cut(s) 21, 127, 151, 280
Cfr10I RCCGGY 1 cut(s) 19
Cfr13I GGNCC 4 cut(s) 123, 159, 177, 178
Csp6I GTAC 2 cut(s) 309, 352
CviAII CATG 2 cut(s) 139, 312
CviQI GTAC 2 cut(s) 309, 352
DdeI CTNAG 4 cut(s) 155, 210, 274, 519
DpnI GATC 2 cut(s) 72, 462
DpnII GATC 2 cut(s) 70, 460
Eco147I AGGCCT 2 cut(s) 153, 208
Eco24I GRGCYC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 159
Eco81I CCTNAGG 2 cut(s) 155, 519
Eco88I CYCGRG 1 cut(s) 94
EcoO109I RGGNCCY 1 cut(s) 159
EcoT22I ATGCAT 1 cut(s) 317
EcoT38I GRGCYC 1 cut(s) 181
FaeI CATG 2 cut(s) 142, 315
FaiI YATR 7 cut(s) 140, 305, 313, 481, 483, 513, 602
FatI CATG 2 cut(s) 138, 311
FblI GTMKAC 1 cut(s) 615
Fnu4HI GCNGC 5 cut(s) 11, 39, 60, 230, 627
FokI GGATG 5 cut(s) 158, 256, 269, 434, 536
FriOI GRGCYC 1 cut(s) 181
Fsp4HI GCNGC 5 cut(s) 11, 39, 60, 230, 627
FspBI CTAG 1 cut(s) 458
GluI GCNGC 5 cut(s) 11, 39, 60, 230, 627
GsaI CCCAGC 2 cut(s) 124, 133
GsuI CTGGAG 1 cut(s) 437
HaeIII GGCC 4 cut(s) 125, 153, 179, 208
HapII CCGG 1 cut(s) 20
Hin1II CATG 2 cut(s) 142, 315
HpaII CCGG 1 cut(s) 20
Hpy166II GTNNAC 1 cut(s) 616
Hpy188I TCNGA 2 cut(s) 370, 495
Hpy188III TCNNGA 5 cut(s) 94, 157, 338, 464, 521
Hpy8I GTNNAC 1 cut(s) 616
HpyCH4III ACNGT 2 cut(s) 250, 439
HpyCH4V TGCA 6 cut(s) 13, 149, 266, 315, 542, 626
HpyF10VI GCNNNNNNNGC 6 cut(s) 16, 59, 126, 185, 548, 635
HpyF3I CTNAG 4 cut(s) 155, 210, 274, 519
Hsp92II CATG 2 cut(s) 142, 315
KroI GCCGGC 1 cut(s) 19
KroNI GCCGGC 1 cut(s) 21
Kzo9I GATC 2 cut(s) 70, 460
LmnI GCTCC 2 cut(s) 388, 404
Lsp1109I GCAGC 2 cut(s) 216, 638
LweI GCATC 5 cut(s) 6, 136, 247, 275, 324
MaeI CTAG 1 cut(s) 458
MalI GATC 2 cut(s) 72, 462
MbiI CCGCTC 1 cut(s) 347
MboI GATC 2 cut(s) 70, 460
MboII GAAGA 2 cut(s) 82, 310
MflI RGATCY 2 cut(s) 70, 460
MhlI GDGCHC 1 cut(s) 181
MluCI AATT 4 cut(s) 508, 579, 621, 633
MmeI TCCRAC 1 cut(s) 410
MnlI CCTC 4 cut(s) 164, 283, 412, 528
Mph1103I ATGCAT 1 cut(s) 317
MroNI GCCGGC 1 cut(s) 19
MroXI GAANNNNTTC 1 cut(s) 375
MseI TTAA 3 cut(s) 293, 582, 652
MspA1I CMGCKG 1 cut(s) 120
MspI CCGG 1 cut(s) 20
MwoI GCNNNNNNNGC 6 cut(s) 16, 59, 126, 185, 548, 635
NaeI GCCGGC 1 cut(s) 21
NdeII GATC 2 cut(s) 70, 460
NgoMIV GCCGGC 1 cut(s) 19
NlaIII CATG 2 cut(s) 142, 315
NlaIV GGNNCC 2 cut(s) 179, 487
NsiI ATGCAT 1 cut(s) 317
NspI RCATGY 1 cut(s) 315
PceI AGGCCT 2 cut(s) 153, 208
PdiI GCCGGC 1 cut(s) 21
PdmI GAANNNNTTC 1 cut(s) 375
PkrI GCNGC 5 cut(s) 12, 40, 61, 231, 628
PpuMI RGGWCCY 1 cut(s) 159
Psp5II RGGWCCY 1 cut(s) 159
PspFI CCCAGC 2 cut(s) 120, 129
PspN4I GGNNCC 2 cut(s) 179, 487
PspOMI GGGCCC 1 cut(s) 177
PspPI GGNCC 4 cut(s) 123, 159, 177, 178
PspPPI RGGWCCY 1 cut(s) 159
PsrI GAACNNNNNNTAC 4 cut(s) 292, 324, 438, 470
PstI CTGCAG 1 cut(s) 15
PsuI RGATCY 2 cut(s) 70, 460
PvuII CAGCTG 1 cut(s) 120
RsaI GTAC 2 cut(s) 310, 353
RsaNI GTAC 2 cut(s) 309, 352
SaqAI TTAA 3 cut(s) 293, 582, 652
SatI GCNGC 5 cut(s) 11, 39, 60, 230, 627
Sau3AI GATC 2 cut(s) 70, 460
Sau96I GGNCC 4 cut(s) 123, 159, 177, 178
SduI GDGCHC 1 cut(s) 181
SfaNI GCATC 5 cut(s) 6, 136, 247, 275, 324
SfcI CTRYAG 1 cut(s) 11
SinI GGWCC 1 cut(s) 159
Sse9I AATT 4 cut(s) 508, 579, 621, 633
SseBI AGGCCT 2 cut(s) 153, 208
SsiI CCGC 3 cut(s) 38, 59, 347
SspMI CTAG 1 cut(s) 458
StuI AGGCCT 2 cut(s) 153, 208
TaaI ACNGT 2 cut(s) 250, 439
TaqI TCGA 1 cut(s) 644
TasI AATT 4 cut(s) 508, 579, 621, 633
TatI WGTACW 2 cut(s) 308, 351
TauI GCSGC 2 cut(s) 41, 62
Tru1I TTAA 3 cut(s) 293, 582, 652
Tru9I TTAA 3 cut(s) 293, 582, 652
TscAI CASTG 2 cut(s) 69, 111
TseI GCWGC 3 cut(s) 10, 229, 626
TspDTI ATGAA 2 cut(s) 127, 310
TspRI CASTG 2 cut(s) 69, 111
VpaK11BI GGWCC 1 cut(s) 159
XceI RCATGY 1 cut(s) 315
XmiI GTMKAC 1 cut(s) 615
XmnI GAANNNNTTC 1 cut(s) 375
XspI CTAG 1 cut(s) 458
Zsp2I ATGCAT 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.