Rw4G010330

FHA domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002746
Physical Location & Seq
Forward (+)
23897694 .. 23904813
7120 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G010330.1

Sequence Viewer

Length: 1314 bp
ATGGGGCGTAATCTAAAAAATCATTCCGAATCTCCAGTACGTGACCATGGATCTCTTCCTAGAAGGAGCCCTTATAGGAAGAGTCAATCTCGAACAGAAAGATCACCTAATCAACATAGAAAGTCCCAGAGAGGCAGTTCTCCCGTTAGAGAGAAGCATTCAAGTCGCCCCAAGTCTCCCAAGCATGCAAGATCGCCCACTCCTCCTCCTCGCTCTCCTTCTCCTCGCACCAAATGGTTAAGAAGAGCTCAAGCTGGAAGAGTGCCTGAGAGAGAACATGAAAGGAAGAATGGTAGGGGGATCGGTAGGGATTTACTGGAGGGGGGTCCAGAGAGAGAAGTTGGGAGTGATAGAAATGAGAAATTGTCAGGAAGGGATCATACCGATGGTAAATCTTCAAGATCAAGGCATGTTACCTCTCCATCAGATCAACACCGGAGGAGTAGGCATAGATCTCCCTCACCTCAACATCCTGATGAAACAAAATATGGAGATAAAGTAATTGAGGGCCATGAAAGAAATCATCACAGAGGGAGTGATAGAAGTATGCAAAGGGAAAAGGGCTCAGATAGGGAAAATGAGACAGAAGGAATGGAGAGAGGGACAGAAAAAGACAGTTCTGGTCATAGGTCTTCAAGATCGAGACATGGGGAGTCTACTTCACCATTGAATGGACACCAGGAGAAAAAACACAAAACTCATTCGCCTCGACAAGCTGCAGATACCAGAGCTCACTATGAGGTGCCAGAGTCAAGGGAAGCTGAGCATGGGATTGATGAGAATGATTCAGTAGCAATGATGAGAGCTGCTGAGGAGGCCTTGGAAGCACAGAAAAAGCAAAAACCTTCATTTGAGCTATCTGGAAAGCTTGCTGCGGAAACCAATAGATTCAGGGGTATAACACTATTATACACTGAGCCAGCAGAAGGACGGCCACCTCGTGTAAAATGGCGGCTTTTTATGTTTTTAAGGGTGGTGAAGCTTTTAATGGCAAAACCCATTAAGATACATCGCCAAAGCTGTTACCTTTTCGGGAGGGAAAGAAGGGTGGCAGACATCCCTACGGATCACCCATCCTGCAGCAAGCAACATGCTGTTCTACAATTCCGGCAAGTAGAAAAGGAGCAACCAGACGGTACATTGTCAAAGGAAGTCAAGCCTTACGTAATGGATCTTGGAAGCACAAATAAAACTTTTGTCAATGAAACTGCAATTGAACCTCAGCGATATTATGAACTTATGGAAAAGGACACGATTAAATTTGGTAATAGTAGCCGAGAGTATGTTCTACTGCACAAGAATTCTATGGATTGA

Protein Analysis

437

Amino Acids

50.27

Weight (kDa)

10.1

Isoelectric Point (pI)

64.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FHA PF00498 342 - 422 1.1e-15 FHA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 742
AccB7I CCANNNNNTGG 1 cut(s) 671
AccI GTMKAC 1 cut(s) 656
AciI CCGC 2 cut(s) 875, 952
AclWI GGATC 5 cut(s) 58, 308, 384, 1074, 1179
AcoI YGGCCR 1 cut(s) 932
AcsI RAATTY 2 cut(s) 1259, 1300
AdeI CACNNNGTG 1 cut(s) 941
AfaI GTAC 2 cut(s) 39, 1138
AfiI CCNNNNNNNGG 2 cut(s) 671, 926
AgsI TTSAA 5 cut(s) 162, 399, 636, 670, 1217
AjnI CCWGG 1 cut(s) 678
Alw21I GWGCWC 2 cut(s) 250, 733
Alw26I GTCTC 3 cut(s) 180, 575, 637
AlwI GGATC 5 cut(s) 58, 308, 384, 1074, 1179
AoxI GGCC 3 cut(s) 508, 816, 932
ApeKI GCWGC 4 cut(s) 716, 806, 872, 1080
ApoI RAATTY 2 cut(s) 1259, 1300
AspS9I GGNCC 2 cut(s) 326, 508
AsuHPI GGTGA 5 cut(s) 96, 453, 654, 988, 1061
AvaII GGWCC 1 cut(s) 326
BanI GGYRCC 1 cut(s) 742
BanII GRGCYC 4 cut(s) 71, 250, 566, 733
BarI GAAGNNNNNNTAC 2 cut(s) 364, 396
BauI CACGAG 1 cut(s) 939
BbsI GAAGAC 1 cut(s) 624
Bbv12I GWGCWC 2 cut(s) 250, 733
BbvCI CCTCAGC 2 cut(s) 810, 1221
BbvI GCAGC 4 cut(s) 703, 793, 859, 1092
BccI CCATC 3 cut(s) 380, 430, 1081
BceAI ACGGC 1 cut(s) 947
BcgI CGANNNNNNTGC 2 cut(s) 146, 180
BciT130I CCWGG 1 cut(s) 680
BcoDI GTCTC 3 cut(s) 180, 575, 637
BfaI CTAG 1 cut(s) 60
BfmI CTRYAG 2 cut(s) 717, 1078
BglII AGATCT 1 cut(s) 452
BisI GCNGC 5 cut(s) 717, 807, 873, 953, 1081
BlpI GCTNAGC 1 cut(s) 762
BlsI GCNGC 5 cut(s) 718, 808, 874, 954, 1082
Bme1390I CCNGG 1 cut(s) 680
Bme18I GGWCC 1 cut(s) 326
BmgT120I GGNCC 2 cut(s) 326, 508
BmiI GGNNCC 3 cut(s) 68, 327, 744
BmrFI CCNGG 1 cut(s) 680
BpiI GAAGAC 1 cut(s) 624
BplI GAGNNNNNCTC 2 cut(s) 73, 105
BpmI CTGGAG 2 cut(s) 18, 338
Bpu10I CCTNAGC 2 cut(s) 810, 1221
Bpu1102I GCTNAGC 1 cut(s) 762
BpuEI CTTGAG 1 cut(s) 234
BsaAI YACGTR 2 cut(s) 41, 1165
BsaJI CCNNGG 2 cut(s) 46, 819
BsaWI WCCGGW 1 cut(s) 435
BsaXI ACNNNNNCTCC 4 cut(s) 190, 220, 483, 513
Bsc4I CCNNNNNNNGG 2 cut(s) 671, 926
Bse1I ACTGG 2 cut(s) 35, 321
Bse3DI GCAATG 1 cut(s) 801
BseBI CCWGG 1 cut(s) 680
BseDI CCNNGG 2 cut(s) 46, 819
BseGI GGATG 3 cut(s) 469, 1056, 1073
BseLI CCNNNNNNNGG 2 cut(s) 671, 926
BseMI GCAATG 1 cut(s) 801
BseMII CTCAG 6 cut(s) 258, 579, 753, 801, 906, 1235
BseNI ACTGG 2 cut(s) 35, 321
BseRI GAGGAG 6 cut(s) 192, 195, 198, 213, 454, 827
BseXI GCAGC 4 cut(s) 703, 793, 859, 1092
BsgI GTGCAG 1 cut(s) 1277
BshFI GGCC 3 cut(s) 510, 818, 934
BshNI GGYRCC 1 cut(s) 742
BsiHKAI GWGCWC 2 cut(s) 250, 733
BsiSI CCGG 2 cut(s) 436, 1108
BslFI GGGAC 2 cut(s) 109, 616
BslI CCNNNNNNNGG 2 cut(s) 671, 926
BsmAI GTCTC 3 cut(s) 180, 575, 637
BsmFI GGGAC 2 cut(s) 109, 616
BsmI GAATGC 1 cut(s) 157
BsnI GGCC 3 cut(s) 510, 818, 934
Bsp1286I GDGCHC 4 cut(s) 71, 250, 566, 733
Bsp1720I GCTNAGC 1 cut(s) 762
Bsp19I CCATGG 1 cut(s) 46
BspACI CCGC 2 cut(s) 875, 952
BspANI GGCC 3 cut(s) 510, 818, 934
BspCNI CTCAG 6 cut(s) 259, 578, 754, 802, 907, 1234
BspLI GGNNCC 3 cut(s) 68, 327, 744
BspMAI CTGCAG 2 cut(s) 721, 1082
BspPI GGATC 5 cut(s) 58, 308, 384, 1074, 1179
BspQI GCTCTTC 1 cut(s) 238
BspT107I GGYRCC 1 cut(s) 742
BsrDI GCAATG 1 cut(s) 801
BsrI ACTGG 2 cut(s) 35, 321
BssECI CCNNGG 2 cut(s) 46, 819
BssSI CACGAG 1 cut(s) 939
BssT1I CCWWGG 2 cut(s) 46, 819
Bst2BI CACGAG 1 cut(s) 939
Bst2UI CCWGG 1 cut(s) 680
Bst4CI ACNGT 2 cut(s) 617, 1136
Bst6I CTCTTC 4 cut(s) 60, 74, 238, 253
BstBAI YACGTR 2 cut(s) 41, 1165
BstC8I GCNNGC 4 cut(s) 186, 870, 921, 1085
BstDEI CTNAG 6 cut(s) 267, 565, 762, 810, 915, 1221
BstDSI CCRYGG 1 cut(s) 46
BstF5I GGATG 3 cut(s) 469, 1056, 1073
BstMAI GTCTC 3 cut(s) 180, 575, 637
BstMWI GCNNNNNNNGC 2 cut(s) 815, 824
BstNI CCWGG 1 cut(s) 680
BstNSI RCATGY 3 cut(s) 188, 413, 1094
BstSCI CCNGG 1 cut(s) 678
BstSFI CTRYAG 2 cut(s) 717, 1078
BstSNI TACGTA 1 cut(s) 1165
BstV1I GCAGC 4 cut(s) 703, 793, 859, 1092
BstV2I GAAGAC 1 cut(s) 624
BstX2I RGATCY 3 cut(s) 50, 452, 1171
BstYI RGATCY 3 cut(s) 50, 452, 1171
BsuRI GGCC 3 cut(s) 510, 818, 934
BtgI CCRYGG 1 cut(s) 46
BtgZI GCGATG 1 cut(s) 995
BtsCI GGATG 3 cut(s) 469, 1056, 1073
BtsIMutI CAGTG 1 cut(s) 912
Cac8I GCNNGC 4 cut(s) 186, 870, 921, 1085
Cfr13I GGNCC 2 cut(s) 326, 508
Csp6I GTAC 2 cut(s) 38, 1137
CviAII CATG 8 cut(s) 47, 185, 278, 410, 512, 647, 767, 1091
CviQI GTAC 2 cut(s) 38, 1137
DdeI CTNAG 6 cut(s) 267, 565, 762, 810, 915, 1221
DraIII CACNNNGTG 1 cut(s) 941
EaeI YGGCCR 1 cut(s) 932
Eam1104I CTCTTC 4 cut(s) 60, 74, 238, 253
EarI CTCTTC 4 cut(s) 60, 74, 238, 253
Ecl136II GAGCTC 2 cut(s) 248, 731
Eco105I TACGTA 1 cut(s) 1165
Eco130I CCWWGG 2 cut(s) 46, 819
Eco147I AGGCCT 1 cut(s) 818
Eco24I GRGCYC 4 cut(s) 71, 250, 566, 733
Eco47I GGWCC 1 cut(s) 326
Eco53kI GAGCTC 2 cut(s) 248, 731
EcoICRI GAGCTC 2 cut(s) 248, 731
EcoRI GAATTC 1 cut(s) 1300
EcoRII CCWGG 1 cut(s) 678
EcoT14I CCWWGG 2 cut(s) 46, 819
EcoT38I GRGCYC 4 cut(s) 71, 250, 566, 733
ErhI CCWWGG 2 cut(s) 46, 819
FaeI CATG 8 cut(s) 50, 188, 281, 413, 515, 650, 770, 1094
FalI AAGNNNNNCTT 2 cut(s) 55, 87
FaqI GGGAC 2 cut(s) 109, 616
FatI CATG 8 cut(s) 46, 184, 277, 409, 511, 646, 766, 1090
FblI GTMKAC 1 cut(s) 656
Fnu4HI GCNGC 5 cut(s) 717, 807, 873, 953, 1081
FokI GGATG 3 cut(s) 456, 1043, 1060
FriOI GRGCYC 4 cut(s) 71, 250, 566, 733
Fsp4HI GCNGC 5 cut(s) 717, 807, 873, 953, 1081
FspBI CTAG 1 cut(s) 60
GluI GCNGC 5 cut(s) 717, 807, 873, 953, 1081
GsuI CTGGAG 2 cut(s) 18, 338
HaeIII GGCC 3 cut(s) 510, 818, 934
HapII CCGG 2 cut(s) 436, 1108
Hin1II CATG 8 cut(s) 50, 188, 281, 413, 515, 650, 770, 1094
HindIII AAGCTT 2 cut(s) 866, 980
HinfI GANTC 6 cut(s) 29, 82, 653, 749, 785, 888
HpaII CCGG 2 cut(s) 436, 1108
HphI GGTGA 5 cut(s) 96, 453, 654, 988, 1061
Hpy166II GTNNAC 1 cut(s) 657
Hpy188I TCNGA 3 cut(s) 28, 427, 568
Hpy188III TCNNGA 9 cut(s) 90, 329, 369, 399, 473, 636, 642, 861, 1033
Hpy8I GTNNAC 1 cut(s) 657
HpyAV CCTTC 7 cut(s) 57, 228, 366, 581, 855, 920, 1038
HpyCH4III ACNGT 2 cut(s) 617, 1136
HpyCH4IV ACGT 2 cut(s) 40, 1164
HpyCH4V TGCA 6 cut(s) 188, 550, 719, 1080, 1211, 1294
HpyF10VI GCNNNNNNNGC 2 cut(s) 815, 824
HpyF3I CTNAG 6 cut(s) 267, 565, 762, 810, 915, 1221
HpySE526I ACGT 2 cut(s) 40, 1164
Hsp92II CATG 8 cut(s) 50, 188, 281, 413, 515, 650, 770, 1094
LguI GCTCTTC 1 cut(s) 238
LmnI GCTCC 2 cut(s) 66, 1123
Lsp1109I GCAGC 4 cut(s) 703, 793, 859, 1092
MaeI CTAG 1 cut(s) 60
MaeII ACGT 2 cut(s) 40, 1164
MaeIII GTNAC 3 cut(s) 41, 412, 1022
MboII GAAGA 7 cut(s) 47, 91, 255, 270, 298, 387, 624
MfeI CAATTG 1 cut(s) 1212
MflI RGATCY 3 cut(s) 50, 452, 1171
MhlI GDGCHC 4 cut(s) 71, 250, 566, 733
MluCI AATT 6 cut(s) 362, 501, 1103, 1212, 1259, 1300
MlyI GAGTC 3 cut(s) 91, 662, 758
MseI TTAA 5 cut(s) 239, 968, 986, 1002, 1257
MslI CAYNNNNRTG 2 cut(s) 384, 474
MspI CCGG 2 cut(s) 436, 1108
MspR9I CCNGG 1 cut(s) 680
MunI CAATTG 1 cut(s) 1212
Mva1269I GAATGC 1 cut(s) 157
MvaI CCWGG 1 cut(s) 680
MwoI GCNNNNNNNGC 2 cut(s) 815, 824
NcoI CCATGG 1 cut(s) 46
NlaIII CATG 8 cut(s) 50, 188, 281, 413, 515, 650, 770, 1094
NlaIV GGNNCC 3 cut(s) 68, 327, 744
NmeAIII GCCGAG 1 cut(s) 1301
NmuCI GTSAC 1 cut(s) 41
NspI RCATGY 3 cut(s) 188, 413, 1094
PaeI GCATGC 1 cut(s) 188
PceI AGGCCT 1 cut(s) 818
PciSI GCTCTTC 1 cut(s) 238
PcsI WCGNNNNNNNCGW 1 cut(s) 937
PctI GAATGC 1 cut(s) 157
PfeI GAWTC 3 cut(s) 29, 785, 888
PflMI CCANNNNNTGG 1 cut(s) 671
PkrI GCNGC 5 cut(s) 718, 808, 874, 954, 1082
PleI GAGTC 3 cut(s) 90, 661, 757
PpsI GAGTC 3 cut(s) 90, 661, 757
Ppu21I YACGTR 2 cut(s) 41, 1165
Psp124BI GAGCTC 2 cut(s) 250, 733
Psp6I CCWGG 1 cut(s) 678
PspGI CCWGG 1 cut(s) 678
PspN4I GGNNCC 3 cut(s) 68, 327, 744
PspPI GGNCC 2 cut(s) 326, 508
PstI CTGCAG 2 cut(s) 721, 1082
PsuI RGATCY 3 cut(s) 50, 452, 1171
RsaI GTAC 2 cut(s) 39, 1138
RsaNI GTAC 2 cut(s) 38, 1137
RseI CAYNNNNRTG 2 cut(s) 384, 474
SacI GAGCTC 2 cut(s) 250, 733
SapI GCTCTTC 1 cut(s) 238
SaqAI TTAA 5 cut(s) 239, 968, 986, 1002, 1257
SatI GCNGC 5 cut(s) 717, 807, 873, 953, 1081
Sau96I GGNCC 2 cut(s) 326, 508
SchI GAGTC 3 cut(s) 91, 662, 758
ScrFI CCNGG 1 cut(s) 680
SduI GDGCHC 4 cut(s) 71, 250, 566, 733
SfcI CTRYAG 2 cut(s) 717, 1078
SinI GGWCC 1 cut(s) 326
SmiMI CAYNNNNRTG 2 cut(s) 384, 474
SmlI CTYRAG 1 cut(s) 249
SmoI CTYRAG 1 cut(s) 249
SnaBI TACGTA 1 cut(s) 1165
SphI GCATGC 1 cut(s) 188
Sse9I AATT 6 cut(s) 362, 501, 1103, 1212, 1259, 1300
SseBI AGGCCT 1 cut(s) 818
SsiI CCGC 2 cut(s) 875, 952
SspMI CTAG 1 cut(s) 60
SstI GAGCTC 2 cut(s) 250, 733
StuI AGGCCT 1 cut(s) 818
StyD4I CCNGG 1 cut(s) 678
StyI CCWWGG 2 cut(s) 46, 819
TaaI ACNGT 2 cut(s) 617, 1136
TaiI ACGT 2 cut(s) 43, 1167
TaqI TCGA 3 cut(s) 91, 641, 709
TasI AATT 6 cut(s) 362, 501, 1103, 1212, 1259, 1300
TauI GCSGC 1 cut(s) 955
TfiI GAWTC 3 cut(s) 29, 785, 888
Tru1I TTAA 5 cut(s) 239, 968, 986, 1002, 1257
Tru9I TTAA 5 cut(s) 239, 968, 986, 1002, 1257
TscAI CASTG 1 cut(s) 919
TseFI GTSAC 1 cut(s) 41
TseI GCWGC 4 cut(s) 716, 806, 872, 1080
Tsp45I GTSAC 1 cut(s) 41
TspDTI ATGAA 6 cut(s) 294, 492, 528, 837, 1218, 1248
TspGWI ACGGA 1 cut(s) 1079
TspRI CASTG 1 cut(s) 919
Van91I CCANNNNNTGG 1 cut(s) 671
VpaK11BI GGWCC 1 cut(s) 326
XapI RAATTY 2 cut(s) 1259, 1300
XceI RCATGY 3 cut(s) 188, 413, 1094
XmiI GTMKAC 1 cut(s) 656
XspI CTAG 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.