AT1G01710

acyl-CoA thioesterase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003309
Physical Location & Seq
Forward (+)
262828 .. 266324
3497 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G01710.1

Sequence Viewer

Length: 1284 bp
ATGAACACCGAGTCAGTTGTCGAGTTCCTTGGAAACGTCCCTTTACTCCAGAAATTACCGAGCTCTTCTCTGAAGAAAATTGCTCAAGTTGTTGTGCCGAAACGTTACGGGAAAGGGGATTATGTGGTTCGTGAAGATCAAACTTGGGATGGATGTTATTTTATTTTGCAAGGAGAGGCTCAGGTTTCCGGACCAGACGAAGAAGATAACCGTTCCGAATTCCTCTTGAAACAGTATGATTACTTCGGCGTTGGTTTATCTGGGAACGTTCATTCAGCAGACATTGTTGCTATGTCCCAGCTTACGTGTTTGGTGTTGCCGCGTGATCACTGTCATTTGCTTGAGACTAATTCCATCTGGCAATCGGATACGTCACTTGACAAATGCTCTTTAGTGGAACGCATTTTGCAACTCGACCCTTTAGAATTGAATATCTTCAGGGGGATCACACTACCTGATGCTCCTATATTTGGAAAGGTTTTTGGAGGACAATTTGTCGGACAGGCTCTTGCTGCGGCGTCAAAAACTGTTGATTTTCTCAAGGTCGTCCACAGTTTACACTCCTATTTTCTTCTCGTTGGAGATATTGACATCCCCATTATTTATCAAGTTCACCGCATACGTGATGGGAACAACTTTGCCACCCGAAGAGTTGATGCAGTACAGAAAGGAAATATCATATTCATCTTGCTGGCATCATTTCAGAAAGAACAACAAGGATTTGAGCACCAGGAGTCAACCATGCCCTCTGTACCAGATCCTGATACGCTTCTATCACTGGAGGAGTTGCGTGAAAGCCGTATAACTGATCCTCATTTACCGAGGAGTTATCGGAACAAAGTTGCAACTAGAAACTTTGTTCCATGGCCTATAGAGATTCGATTTTGTGAGCCCAGCAATTCAACAAATCAGACAAAGTCTCCTCCGAGATTGAACTATTGGTTTAGAGCAAAGGGAAGGCTTTCTGATGATCAAGCTTTGCACCGATGTGTCGTTGCATTTGCCTCGGATTTAATATTTTGTGGTGTTGGTTTGAACCCTCACCGGAGAAAAGGGGTGAAATCCGCCGCACTTAGCCTGGACCATGCGATGTGGTTTCACCGACCTCTAAGAGCCGACGAGTGGTTGCTCTATGTGATTGTGAGTCCAACTGCGCATGAGACCCGTGGTTTTGTGACTGGTCAAATGTTCAACAGGAAAGGAGAGCTGGTTGTATCATTAACGCAAGAGGCCCTATTAAGAGAGGCTAGACCTCCAAAACCCTCCGGCACGTCGAAGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0006082 GO:0006139 GO:0006163 GO:0006629 GO:0006631 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009117 GO:0009150 GO:0009259 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010559 GO:0010561 GO:0010605 GO:0016042 GO:0016043 GO:0016054 GO:0016289 GO:0016290 GO:0016559 GO:0016787 GO:0016788 GO:0016790 GO:0019222 GO:0019637 GO:0019693 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031907 GO:0031974 GO:0032787 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043648 GO:0043649 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045223 GO:0045225 GO:0046395 GO:0046483 GO:0047617 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051186 GO:0051246 GO:0051248 GO:0052815 GO:0055086 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072521 GO:0080090 GO:1901135 GO:1901360 GO:1901564 GO:1901575 GO:1903018 GO:1903019 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01100 ko01110 map00062 map01040 map01100 map01110
Pfam Domains
Protein Families
4HBT_3 Acyl_CoA_thio cNMP_binding

Protein Analysis

427

Amino Acids

48.15

Weight (kDa)

7.21

Isoelectric Point (pI)

33.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cNMP_binding PF00027 34 - 110 2e-10 Cyclic nucleotide-binding domain
4HBT_3 PF13622 158 - 235 1.2e-15 Acyl-CoA thioesterase N-terminal domain
Acyl_CoA_thio PF02551 293 - 410 1.3e-24 Acyl-CoA thioesterase
4HBT_3C PF20789 298 - 412 1.6e-24 Acyl-CoA thioesterase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1155
AccII CGCG 1 cut(s) 322
AccIII TCCGGA 1 cut(s) 188
AciI CCGC 5 cut(s) 320, 515, 616, 1065, 1068
AclI AACGTT 2 cut(s) 103, 267
AclWI GGATC 3 cut(s) 452, 752, 803
AcsI RAATTY 1 cut(s) 218
AcuI CTGAAG 2 cut(s) 92, 421
AcyI GRCGYC 1 cut(s) 518
AfaI GTAC 2 cut(s) 663, 753
AfiI CCNNNNNNNGG 2 cut(s) 470, 1122
AflIII ACRYGT 1 cut(s) 305
AgsI TTSAA 6 cut(s) 229, 430, 903, 934, 1036, 1192
AhdI GACNNNNNGTC 1 cut(s) 494
AjiI CACGTC 1 cut(s) 1272
AjnI CCWGG 2 cut(s) 729, 1077
AleI CACNNNNGTG 1 cut(s) 987
AluBI AGCT 5 cut(s) 63, 301, 977, 1207, 1279
AluI AGCT 5 cut(s) 63, 301, 977, 1207, 1279
Alw21I GWGCWC 2 cut(s) 65, 729
Alw26I GTCTC 3 cut(s) 338, 924, 1154
AlwI GGATC 3 cut(s) 452, 752, 803
AlwNI CAGNNNCTG 1 cut(s) 761
Aor13HI TCCGGA 1 cut(s) 188
AoxI GGCC 2 cut(s) 866, 1230
ApeKI GCWGC 1 cut(s) 512
ApoI RAATTY 1 cut(s) 218
Asp700I GAANNNNTTC 2 cut(s) 434, 961
AspLEI GCGC 1 cut(s) 1156
AspS9I GGNCC 3 cut(s) 191, 1081, 1231
AsuHPI GGTGA 4 cut(s) 605, 1034, 1069, 1091
AvaII GGWCC 2 cut(s) 191, 1081
BanII GRGCYC 2 cut(s) 65, 894
BarI GAAGNNNNNNTAC 2 cut(s) 227, 259
Bbv12I GWGCWC 2 cut(s) 65, 729
BbvI GCAGC 1 cut(s) 499
BccI CCATC 3 cut(s) 143, 362, 620
BceAI ACGGC 1 cut(s) 783
BcgI CGANNNNNNTGC 2 cut(s) 987, 1021
BciT130I CCWGG 2 cut(s) 731, 1079
BciVI GTATCC 1 cut(s) 361
BclI TGATCA 2 cut(s) 325, 970
BcoDI GTCTC 3 cut(s) 338, 924, 1154
BfaI CTAG 2 cut(s) 849, 1248
BfmI CTRYAG 1 cut(s) 870
BfuI GTATCC 1 cut(s) 361
BisI GCNGC 4 cut(s) 320, 513, 516, 1068
BlsI GCNGC 4 cut(s) 321, 514, 517, 1069
Bme1390I CCNGG 2 cut(s) 731, 1079
Bme18I GGWCC 2 cut(s) 191, 1081
BmeRI GACNNNNNGTC 1 cut(s) 494
BmgBI CACGTC 1 cut(s) 1272
BmgT120I GGNCC 3 cut(s) 191, 1081, 1231
BmrFI CCNGG 2 cut(s) 731, 1079
BmsI GCATC 3 cut(s) 448, 646, 704
BplI GAGNNNNNCTC 2 cut(s) 52, 84
BpmI CTGGAG 2 cut(s) 32, 800
Bpu10I CCTNAGC 1 cut(s) 180
BpuEI CTTGAG 3 cut(s) 69, 362, 524
BsaAI YACGTR 2 cut(s) 306, 623
BsaHI GRCGYC 1 cut(s) 518
BsaI GGTCTC 1 cut(s) 1154
BsaJI CCNNGG 5 cut(s) 28, 821, 863, 1005, 1165
BsaWI WCCGGW 2 cut(s) 188, 1044
BsaXI ACNNNNNCTCC 4 cut(s) 904, 934, 1194, 1224
Bsc4I CCNNNNNNNGG 2 cut(s) 470, 1122
Bse1I ACTGG 2 cut(s) 783, 1183
BseAI TCCGGA 1 cut(s) 188
BseBI CCWGG 2 cut(s) 731, 1079
BseDI CCNNGG 5 cut(s) 28, 821, 863, 1005, 1165
BseGI GGATG 3 cut(s) 154, 158, 591
BseLI CCNNNNNNNGG 2 cut(s) 470, 1122
BseMII CTCAG 1 cut(s) 194
BseNI ACTGG 2 cut(s) 783, 1183
BseRI GAGGAG 3 cut(s) 797, 838, 912
BseXI GCAGC 1 cut(s) 499
BseYI CCCAGC 2 cut(s) 297, 893
Bsh1236I CGCG 1 cut(s) 322
BshFI GGCC 2 cut(s) 868, 1232
BsiHKAI GWGCWC 2 cut(s) 65, 729
BsiSI CCGG 3 cut(s) 189, 1045, 1266
BslFI GGGAC 2 cut(s) 23, 280
BslI CCNNNNNNNGG 2 cut(s) 470, 1122
BsmAI GTCTC 3 cut(s) 338, 924, 1154
BsmFI GGGAC 2 cut(s) 23, 280
BsnI GGCC 2 cut(s) 868, 1232
Bso31I GGTCTC 1 cut(s) 1154
Bsp1286I GDGCHC 3 cut(s) 65, 729, 894
Bsp13I TCCGGA 1 cut(s) 188
Bsp143I GATC 6 cut(s) 136, 325, 444, 757, 808, 970
Bsp19I CCATGG 1 cut(s) 863
BspACI CCGC 5 cut(s) 320, 515, 616, 1065, 1068
BspANI GGCC 2 cut(s) 868, 1232
BspCNI CTCAG 1 cut(s) 193
BspEI TCCGGA 1 cut(s) 188
BspFNI CGCG 1 cut(s) 322
BspPI GGATC 3 cut(s) 452, 752, 803
BspQI GCTCTTC 1 cut(s) 70
BspTNI GGTCTC 1 cut(s) 1154
BsrI ACTGG 2 cut(s) 783, 1183
BssECI CCNNGG 5 cut(s) 28, 821, 863, 1005, 1165
BssMI GATC 6 cut(s) 136, 325, 444, 757, 808, 970
BssNI GRCGYC 1 cut(s) 518
BssT1I CCWWGG 2 cut(s) 28, 863
Bst2UI CCWGG 2 cut(s) 731, 1079
Bst4CI ACNGT 5 cut(s) 212, 234, 332, 529, 554
Bst6I CTCTTC 2 cut(s) 70, 643
BstACI GRCGYC 1 cut(s) 518
BstBAI YACGTR 2 cut(s) 306, 623
BstC8I GCNNGC 1 cut(s) 693
BstDEI CTNAG 3 cut(s) 180, 1073, 1109
BstDSI CCRYGG 2 cut(s) 863, 1165
BstF5I GGATG 3 cut(s) 154, 158, 591
BstFNI CGCG 1 cut(s) 322
BstHHI GCGC 1 cut(s) 1156
BstKTI GATC 6 cut(s) 139, 328, 447, 760, 811, 973
BstMAI GTCTC 3 cut(s) 338, 924, 1154
BstMBI GATC 6 cut(s) 136, 325, 444, 757, 808, 970
BstMWI GCNNNNNNNGC 1 cut(s) 512
BstNI CCWGG 2 cut(s) 731, 1079
BstSCI CCNGG 2 cut(s) 729, 1077
BstSFI CTRYAG 1 cut(s) 870
BstUI CGCG 1 cut(s) 322
BstV1I GCAGC 1 cut(s) 499
BstX2I RGATCY 1 cut(s) 757
BstYI RGATCY 1 cut(s) 757
BsuI GTATCC 1 cut(s) 361
BsuRI GGCC 2 cut(s) 868, 1232
BtgI CCRYGG 2 cut(s) 863, 1165
BtgZI GCGATG 1 cut(s) 1103
BtrI CACGTC 1 cut(s) 1272
BtsCI GGATG 3 cut(s) 154, 158, 591
BtsIMutI CAGTG 2 cut(s) 328, 776
Cac8I GCNNGC 1 cut(s) 693
CaiI CAGNNNCTG 1 cut(s) 761
CfoI GCGC 1 cut(s) 1156
Cfr13I GGNCC 3 cut(s) 191, 1081, 1231
CseI GACGC 1 cut(s) 507
Csp6I GTAC 2 cut(s) 662, 752
CviAII CATG 4 cut(s) 742, 864, 1085, 1157
CviQI GTAC 2 cut(s) 662, 752
DdeI CTNAG 3 cut(s) 180, 1073, 1109
DpnI GATC 6 cut(s) 138, 327, 446, 759, 810, 972
DpnII GATC 6 cut(s) 136, 325, 444, 757, 808, 970
DriI GACNNNNNGTC 1 cut(s) 494
Eam1104I CTCTTC 2 cut(s) 70, 643
Eam1105I GACNNNNNGTC 1 cut(s) 494
EarI CTCTTC 2 cut(s) 70, 643
EciI GGCGGA 1 cut(s) 1054
Ecl136II GAGCTC 1 cut(s) 63
Eco130I CCWWGG 2 cut(s) 28, 863
Eco24I GRGCYC 2 cut(s) 65, 894
Eco31I GGTCTC 1 cut(s) 1154
Eco47I GGWCC 2 cut(s) 191, 1081
Eco53kI GAGCTC 1 cut(s) 63
Eco57I CTGAAG 2 cut(s) 92, 421
EcoICRI GAGCTC 1 cut(s) 63
EcoO109I RGGNCCY 1 cut(s) 1231
EcoRI GAATTC 1 cut(s) 218
EcoRII CCWGG 2 cut(s) 729, 1077
EcoT14I CCWWGG 2 cut(s) 28, 863
EcoT38I GRGCYC 2 cut(s) 65, 894
ErhI CCWWGG 2 cut(s) 28, 863
FaeI CATG 4 cut(s) 745, 867, 1088, 1160
FaqI GGGAC 2 cut(s) 23, 280
FatI CATG 4 cut(s) 741, 863, 1084, 1156
FbaI TGATCA 2 cut(s) 325, 970
Fnu4HI GCNGC 4 cut(s) 320, 513, 516, 1068
FokI GGATG 3 cut(s) 161, 165, 578
FriOI GRGCYC 2 cut(s) 65, 894
Fsp4HI GCNGC 4 cut(s) 320, 513, 516, 1068
FspBI CTAG 2 cut(s) 849, 1248
FspI TGCGCA 1 cut(s) 1155
GlaI GCGC 1 cut(s) 1155
GluI GCNGC 4 cut(s) 320, 513, 516, 1068
GsaI CCCAGC 2 cut(s) 301, 897
GsuI CTGGAG 2 cut(s) 32, 800
HaeIII GGCC 2 cut(s) 868, 1232
HapII CCGG 3 cut(s) 189, 1045, 1266
HgaI GACGC 1 cut(s) 507
HhaI GCGC 1 cut(s) 1156
Hin1I GRCGYC 1 cut(s) 518
Hin1II CATG 4 cut(s) 745, 867, 1088, 1160
Hin6I GCGC 1 cut(s) 1154
HinP1I GCGC 1 cut(s) 1154
HincII GTYRAC 1 cut(s) 738
HindII GTYRAC 1 cut(s) 738
HindIII AAGCTT 1 cut(s) 975
HinfI GANTC 4 cut(s) 11, 734, 877, 1144
HpaII CCGG 3 cut(s) 189, 1045, 1266
HphI GGTGA 4 cut(s) 605, 1034, 1069, 1091
Hpy166II GTNNAC 4 cut(s) 550, 557, 613, 738
Hpy188III TCNNGA 5 cut(s) 49, 131, 189, 226, 761
Hpy8I GTNNAC 4 cut(s) 550, 557, 613, 738
Hpy99I CGWCG 2 cut(s) 1121, 1276
HpyAV CCTTC 1 cut(s) 951
HpyCH4III ACNGT 5 cut(s) 212, 234, 332, 529, 554
HpyCH4IV ACGT 7 cut(s) 36, 103, 267, 305, 371, 622, 1271
HpyCH4V TGCA 6 cut(s) 169, 409, 659, 845, 982, 998
HpyF10VI GCNNNNNNNGC 1 cut(s) 512
HpyF3I CTNAG 3 cut(s) 180, 1073, 1109
HpySE526I ACGT 7 cut(s) 36, 103, 267, 305, 371, 622, 1271
Hsp92I GRCGYC 1 cut(s) 518
Hsp92II CATG 4 cut(s) 745, 867, 1088, 1160
HspAI GCGC 1 cut(s) 1154
Kpn2I TCCGGA 1 cut(s) 188
Ksp22I TGATCA 2 cut(s) 325, 970
Kzo9I GATC 6 cut(s) 136, 325, 444, 757, 808, 970
LguI GCTCTTC 1 cut(s) 70
LmnI GCTCC 1 cut(s) 466
Lsp1109I GCAGC 1 cut(s) 499
LweI GCATC 3 cut(s) 448, 646, 704
MaeI CTAG 2 cut(s) 849, 1248
MaeII ACGT 7 cut(s) 36, 103, 267, 305, 371, 622, 1271
MaeIII GTNAC 3 cut(s) 104, 372, 1174
MalI GATC 6 cut(s) 138, 327, 446, 759, 810, 972
MboI GATC 6 cut(s) 136, 325, 444, 757, 808, 970
MboII GAAGA 8 cut(s) 57, 85, 146, 212, 215, 427, 563, 660
MflI RGATCY 1 cut(s) 757
MhlI GDGCHC 3 cut(s) 65, 729, 894
MluCI AATT 7 cut(s) 53, 78, 218, 349, 425, 491, 898
MlyI GAGTC 3 cut(s) 20, 743, 1153
MmeI TCCRAC 3 cut(s) 478, 559, 1172
MroI TCCGGA 1 cut(s) 188
MroXI GAANNNNTTC 2 cut(s) 434, 961
MseI TTAA 3 cut(s) 1013, 1220, 1238
MslI CAYNNNNRTG 1 cut(s) 987
MspI CCGG 3 cut(s) 189, 1045, 1266
MspR9I CCNGG 2 cut(s) 731, 1079
MvaI CCWGG 2 cut(s) 731, 1079
MvnI CGCG 1 cut(s) 322
MwoI GCNNNNNNNGC 1 cut(s) 512
NcoI CCATGG 1 cut(s) 863
NdeII GATC 6 cut(s) 136, 325, 444, 757, 808, 970
NlaIII CATG 4 cut(s) 745, 867, 1088, 1160
NmuCI GTSAC 2 cut(s) 372, 1174
NsbI TGCGCA 1 cut(s) 1155
OliI CACNNNNGTG 1 cut(s) 987
PciSI GCTCTTC 1 cut(s) 70
PdmI GAANNNNTTC 2 cut(s) 434, 961
PfeI GAWTC 1 cut(s) 877
PflFI GACNNNGTC 1 cut(s) 916
PkrI GCNGC 4 cut(s) 321, 514, 517, 1069
PleI GAGTC 3 cut(s) 19, 742, 1152
PpsI GAGTC 3 cut(s) 19, 742, 1152
Ppu21I YACGTR 2 cut(s) 306, 623
Psp124BI GAGCTC 1 cut(s) 65
Psp1406I AACGTT 2 cut(s) 103, 267
Psp6I CCWGG 2 cut(s) 729, 1077
PspFI CCCAGC 2 cut(s) 297, 893
PspGI CCWGG 2 cut(s) 729, 1077
PspPI GGNCC 3 cut(s) 191, 1081, 1231
PstNI CAGNNNCTG 1 cut(s) 761
PsuI RGATCY 1 cut(s) 757
PsyI GACNNNGTC 1 cut(s) 916
RsaI GTAC 2 cut(s) 663, 753
RsaNI GTAC 2 cut(s) 662, 752
RseI CAYNNNNRTG 1 cut(s) 987
SacI GAGCTC 1 cut(s) 65
SapI GCTCTTC 1 cut(s) 70
SaqAI TTAA 3 cut(s) 1013, 1220, 1238
SatI GCNGC 4 cut(s) 320, 513, 516, 1068
Sau3AI GATC 6 cut(s) 136, 325, 444, 757, 808, 970
Sau96I GGNCC 3 cut(s) 191, 1081, 1231
SchI GAGTC 3 cut(s) 20, 743, 1153
ScrFI CCNGG 2 cut(s) 731, 1079
SduI GDGCHC 3 cut(s) 65, 729, 894
SfaNI GCATC 3 cut(s) 448, 646, 704
SfcI CTRYAG 1 cut(s) 870
SinI GGWCC 2 cut(s) 191, 1081
SmiMI CAYNNNNRTG 1 cut(s) 987
SmlI CTYRAG 3 cut(s) 84, 341, 539
SmoI CTYRAG 3 cut(s) 84, 341, 539
Sse9I AATT 7 cut(s) 53, 78, 218, 349, 425, 491, 898
SsiI CCGC 5 cut(s) 320, 515, 616, 1065, 1068
SspI AATATT 1 cut(s) 1017
SspMI CTAG 2 cut(s) 849, 1248
SstI GAGCTC 1 cut(s) 65
StyD4I CCNGG 2 cut(s) 729, 1077
StyI CCWWGG 2 cut(s) 28, 863
TaaI ACNGT 5 cut(s) 212, 234, 332, 529, 554
TaiI ACGT 7 cut(s) 39, 106, 270, 308, 374, 625, 1274
TaqI TCGA 4 cut(s) 21, 414, 880, 1274
TasI AATT 7 cut(s) 53, 78, 218, 349, 425, 491, 898
TatI WGTACW 1 cut(s) 661
TauI GCSGC 3 cut(s) 322, 518, 1070
TfiI GAWTC 1 cut(s) 877
Tru1I TTAA 3 cut(s) 1013, 1220, 1238
Tru9I TTAA 3 cut(s) 1013, 1220, 1238
TscAI CASTG 2 cut(s) 335, 783
TseFI GTSAC 2 cut(s) 372, 1174
TseI GCWGC 1 cut(s) 512
Tsp45I GTSAC 2 cut(s) 372, 1174
TspDTI ATGAA 3 cut(s) 17, 260, 673
TspRI CASTG 2 cut(s) 335, 783
Tth111I GACNNNGTC 1 cut(s) 916
VpaK11BI GGWCC 2 cut(s) 191, 1081
XapI RAATTY 1 cut(s) 218
XmnI GAANNNNTTC 2 cut(s) 434, 961
XspI CTAG 2 cut(s) 849, 1248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.