Prupe.2G169700_v2.0.a1

Acyl-coenzyme A thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0003309
Physical Location & Seq
Reverse (-)
21707618 .. 21714005
6388 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G169700.2

Sequence Viewer

Length: 1287 bp
ATGGATTCCGAATCAGTGATTGAGTTCCTGGGCTGCGTTCCTCTGCTTCAGAGGCTTCCCAGCTCTTCTCTCAAGAAAGTCGCCCGGCTCGTCATTTTGAAGCACTACGATAGTGGAGAGTATGTTGTTCGTGAAGGTGAAACCGGAGATGGGGTTTACTTCATATGGGATGGGGAGGCTGAAGTTACTGGTTCAGTTAATGCTGATGAAGGAAATCGACCTGAGTTCAAATTGAAACGATACGATTATTTTGGTTATGGTTTTGCAGCATTGGAACATCAGGCTGATGTGATTGCTTTATCTAAGTTGACATGCTTGGTGCTGCCCCATGAACACTGTTCTCTTCTGCAGCCAAAATCAATCTGGAATGCTGATAAAGCTCATGATACGTGCTCTCTTGTTGAGAATATTTTGCATTTGGAACCAATTGAAGTAAACATATTTCAGGGGATTACTTTGCCAGATGCACCAAGATTCGGACAGGTTTTTGGAGGACAGTTAGTTGGACAGGCATTGGCTGCAGCAACGAAAACTGTTGATTGTCTTAAACTTGTCCATAGTTTGCATGCGCATTTTCTGCTTGTTGGGGATTTTAACACACCAATTATATATCAAGTTCACCGAGTACGTGATGGGAAGAGCTTTGCTACTCGAAGAGTGGACGCAATACAAAAAGGGATTAACATTTTCACGATACTGGCTTCATTTAAAAAAGAAGAAGAAGGGTCTGTCCATCAGGAAGCAAAAATGCCATCCGTGCCTGATCCAGATACGCTTCTGTCAATGGAAGAATTACGTGAAAAACGTCTCCTTGATCCGCGTCTTCCAAGGATTTACCGAATCAAGGCAGCTGCAAAGAAATTTGTCCCTTGGCCTATAGAGATACGATTTTGTGAGCCTAGCAGCTCAACCAATCAGACTAAATCCCCACCAAGTTTGAGGTTCTGGTTTAGAGCAAAAGGAAAACTTTCAGATGATCAGGCTTTGCATAGATGTGTTGTAGCATATGCTTCAGATTTGATATTTTCTGGTGTCAGTTTGAATCCCCATCGTAGAAGGGGAGTTAAGTCATCCCAAGTTAGTTTGGACCACGCGATGTGGTTTCACCGCCCAGTTAGAGCTGATGACTGGTTACTCTATGTGATAGTAAGTCCCATTGCCTATAATGCACGCGGATTTGTTTCAGGTCAAATGTTTAATAGAAATGGAGAGCTCATTGTTTCATTGACTCAAGAGTCCCTGAATAGGCCAATTAGACCAATTTCTGCTCCCACATCTAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0006082 GO:0006139 GO:0006163 GO:0006629 GO:0006631 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009117 GO:0009150 GO:0009259 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010559 GO:0010561 GO:0010605 GO:0016042 GO:0016043 GO:0016054 GO:0016289 GO:0016290 GO:0016559 GO:0016787 GO:0016788 GO:0016790 GO:0019222 GO:0019637 GO:0019693 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031907 GO:0031974 GO:0032787 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043648 GO:0043649 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045223 GO:0045225 GO:0046395 GO:0046483 GO:0047617 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051186 GO:0051246 GO:0051248 GO:0052815 GO:0055086 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072521 GO:0080090 GO:1901135 GO:1901360 GO:1901564 GO:1901575 GO:1903018 GO:1903019 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01100 ko01110 map00062 map01040 map01100 map01110
Pfam Domains
Protein Families
4HBT_3 Acyl_CoA_thio cNMP_binding

Protein Analysis

429

Amino Acids

48.1

Weight (kDa)

8.55

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1234
Acc16I TGCGCA 1 cut(s) 570
AccII CGCG 3 cut(s) 820, 1094, 1173
AciI CCGC 3 cut(s) 818, 1108, 1173
AclWI GGATC 2 cut(s) 758, 809
AcsI RAATTY 1 cut(s) 860
AcuI CTGAAG 3 cut(s) 32, 201, 996
AfaI GTAC 1 cut(s) 627
AfiI CCNNNNNNNGG 4 cut(s) 150, 476, 844, 1245
AgsI TTSAA 5 cut(s) 100, 229, 235, 431, 1042
AjnI CCWGG 1 cut(s) 27
AluBI AGCT 7 cut(s) 63, 380, 642, 851, 906, 1121, 1213
AluI AGCT 7 cut(s) 63, 380, 642, 851, 906, 1121, 1213
Alw21I GWGCWC 2 cut(s) 395, 1215
Alw26I GTCTC 1 cut(s) 812
AlwI GGATC 2 cut(s) 758, 809
AoxI GGCC 2 cut(s) 872, 1247
ApeKI GCWGC 9 cut(s) 33, 266, 322, 349, 518, 521, 848, 851, 903
ApoI RAATTY 1 cut(s) 860
Asp700I GAANNNNTTC 1 cut(s) 967
AspLEI GCGC 1 cut(s) 571
AspS9I GGNCC 1 cut(s) 1087
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 3 cut(s) 149, 611, 1097
AvaII GGWCC 1 cut(s) 1087
BanII GRGCYC 1 cut(s) 1215
BbsI GAAGAC 1 cut(s) 815
Bbv12I GWGCWC 2 cut(s) 395, 1215
BbvI GCAGC 9 cut(s) 20, 278, 309, 361, 505, 533, 838, 860, 915
BccI CCATC 6 cut(s) 143, 164, 626, 741, 760, 1056
BciT130I CCWGG 1 cut(s) 29
BclI TGATCA 1 cut(s) 976
BcnI CCSGG 1 cut(s) 85
BcoDI GTCTC 1 cut(s) 812
BfaI CTAG 1 cut(s) 900
BfmI CTRYAG 3 cut(s) 347, 519, 876
BisI GCNGC 9 cut(s) 34, 267, 323, 350, 519, 522, 849, 852, 904
BlsI GCNGC 9 cut(s) 35, 268, 324, 351, 520, 523, 850, 853, 905
Bme1390I CCNGG 2 cut(s) 29, 85
Bme18I GGWCC 1 cut(s) 1087
BmgT120I GGNCC 1 cut(s) 1087
BmiI GGNNCC 1 cut(s) 423
BmrFI CCNGG 2 cut(s) 29, 85
BmrI ACTGGG 1 cut(s) 1106
BmsI GCATC 1 cut(s) 454
BmuI ACTGGG 1 cut(s) 1106
BpiI GAAGAC 1 cut(s) 815
BpuEI CTTGAG 2 cut(s) 56, 1215
BpuMI CCSGG 1 cut(s) 85
BsaAI YACGTR 3 cut(s) 390, 629, 797
BsaJI CCNNGG 3 cut(s) 28, 827, 869
BsaWI WCCGGW 1 cut(s) 143
BsaXI ACNNNNNCTCC 4 cut(s) 108, 138, 138, 168
Bsc4I CCNNNNNNNGG 4 cut(s) 150, 476, 844, 1245
Bse1I ACTGG 4 cut(s) 193, 702, 1112, 1133
Bse3DI GCAATG 1 cut(s) 1155
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 3 cut(s) 28, 827, 869
BseGI GGATG 3 cut(s) 175, 752, 1070
BseLI CCNNNNNNNGG 4 cut(s) 150, 476, 844, 1245
BseMI GCAATG 1 cut(s) 1155
BseMII CTCAG 1 cut(s) 213
BseNI ACTGG 4 cut(s) 193, 702, 1112, 1133
BseXI GCAGC 9 cut(s) 20, 278, 309, 361, 505, 533, 838, 860, 915
BseYI CCCAGC 1 cut(s) 59
Bsh1236I CGCG 3 cut(s) 820, 1094, 1173
BshFI GGCC 2 cut(s) 874, 1249
BsiHKAI GWGCWC 2 cut(s) 395, 1215
BsiSI CCGG 2 cut(s) 85, 144
BslFI GGGAC 3 cut(s) 851, 1137, 1222
BslI CCNNNNNNNGG 4 cut(s) 150, 476, 844, 1245
BsmAI GTCTC 1 cut(s) 812
BsmBI CGTCTC 1 cut(s) 812
BsmFI GGGAC 3 cut(s) 851, 1137, 1222
BsmI GAATGC 1 cut(s) 373
BsnI GGCC 2 cut(s) 874, 1249
Bsp1286I GDGCHC 2 cut(s) 395, 1215
Bsp143I GATC 3 cut(s) 763, 814, 976
BspACI CCGC 3 cut(s) 818, 1108, 1173
BspANI GGCC 2 cut(s) 874, 1249
BspCNI CTCAG 1 cut(s) 214
BspFNI CGCG 3 cut(s) 820, 1094, 1173
BspHI TCATGA 1 cut(s) 382
BspLI GGNNCC 1 cut(s) 423
BspMAI CTGCAG 2 cut(s) 351, 523
BspPI GGATC 2 cut(s) 758, 809
BspQI GCTCTTC 2 cut(s) 70, 632
BsrDI GCAATG 1 cut(s) 1155
BsrI ACTGG 4 cut(s) 193, 702, 1112, 1133
BssECI CCNNGG 3 cut(s) 28, 827, 869
BssMI GATC 3 cut(s) 763, 814, 976
BssT1I CCWWGG 2 cut(s) 827, 869
Bst2UI CCWGG 1 cut(s) 29
Bst4CI ACNGT 3 cut(s) 338, 498, 535
Bst6I CTCTTC 4 cut(s) 70, 348, 632, 649
BstAPI GCANNNNNTGC 2 cut(s) 518, 577
BstBAI YACGTR 3 cut(s) 390, 629, 797
BstC8I GCNNGC 2 cut(s) 567, 1171
BstDEI CTNAG 3 cut(s) 222, 303, 1278
BstF5I GGATG 3 cut(s) 175, 752, 1070
BstFNI CGCG 3 cut(s) 820, 1094, 1173
BstHHI GCGC 1 cut(s) 571
BstKTI GATC 3 cut(s) 766, 817, 979
BstMAI GTCTC 1 cut(s) 812
BstMBI GATC 3 cut(s) 763, 814, 976
BstMWI GCNNNNNNNGC 6 cut(s) 52, 377, 518, 577, 757, 1166
BstNI CCWGG 1 cut(s) 29
BstNSI RCATGY 2 cut(s) 315, 569
BstSCI CCNGG 2 cut(s) 27, 83
BstSFI CTRYAG 3 cut(s) 347, 519, 876
BstUI CGCG 3 cut(s) 820, 1094, 1173
BstV1I GCAGC 9 cut(s) 20, 278, 309, 361, 505, 533, 838, 860, 915
BstV2I GAAGAC 1 cut(s) 815
BsuRI GGCC 2 cut(s) 874, 1249
BtgZI GCGATG 1 cut(s) 1109
BtsCI GGATG 3 cut(s) 175, 752, 1070
BtsIMutI CAGTG 2 cut(s) 21, 334
Cac8I GCNNGC 2 cut(s) 567, 1171
CciI TCATGA 1 cut(s) 382
CfoI GCGC 1 cut(s) 571
Cfr13I GGNCC 1 cut(s) 1087
CseI GACGC 2 cut(s) 671, 809
Csp6I GTAC 1 cut(s) 626
CspCI CAANNNNNGTGG 2 cut(s) 918, 953
CviAII CATG 4 cut(s) 312, 329, 383, 566
CviQI GTAC 1 cut(s) 626
DdeI CTNAG 3 cut(s) 222, 303, 1278
DpnI GATC 3 cut(s) 765, 816, 978
DpnII GATC 3 cut(s) 763, 814, 976
DraI TTTAAA 1 cut(s) 709
DrdI GACNNNNNNGTC 1 cut(s) 1234
DseDI GACNNNNNNGTC 1 cut(s) 1234
Eam1104I CTCTTC 4 cut(s) 70, 348, 632, 649
EarI CTCTTC 4 cut(s) 70, 348, 632, 649
Ecl136II GAGCTC 1 cut(s) 1213
Eco130I CCWWGG 2 cut(s) 827, 869
Eco24I GRGCYC 1 cut(s) 1215
Eco47I GGWCC 1 cut(s) 1087
Eco53kI GAGCTC 1 cut(s) 1213
Eco57I CTGAAG 3 cut(s) 32, 201, 996
EcoICRI GAGCTC 1 cut(s) 1213
EcoRII CCWGG 1 cut(s) 27
EcoT14I CCWWGG 2 cut(s) 827, 869
EcoT38I GRGCYC 1 cut(s) 1215
ErhI CCWWGG 2 cut(s) 827, 869
Esp3I CGTCTC 1 cut(s) 812
FaeI CATG 4 cut(s) 315, 332, 386, 569
FalI AAGNNNNNCTT 2 cut(s) 951, 983
FaqI GGGAC 3 cut(s) 851, 1137, 1222
FatI CATG 4 cut(s) 311, 328, 382, 565
FauNDI CATATG 2 cut(s) 164, 1006
FbaI TGATCA 1 cut(s) 976
Fnu4HI GCNGC 9 cut(s) 34, 267, 323, 350, 519, 522, 849, 852, 904
FokI GGATG 3 cut(s) 182, 739, 1057
FriOI GRGCYC 1 cut(s) 1215
Fsp4HI GCNGC 9 cut(s) 34, 267, 323, 350, 519, 522, 849, 852, 904
FspAI RTGCGCAY 1 cut(s) 570
FspBI CTAG 1 cut(s) 900
FspI TGCGCA 1 cut(s) 570
GlaI GCGC 1 cut(s) 570
GluI GCNGC 9 cut(s) 34, 267, 323, 350, 519, 522, 849, 852, 904
GsaI CCCAGC 1 cut(s) 63
HaeIII GGCC 2 cut(s) 874, 1249
HapII CCGG 2 cut(s) 85, 144
HgaI GACGC 2 cut(s) 671, 809
HhaI GCGC 1 cut(s) 571
Hin1II CATG 4 cut(s) 315, 332, 386, 569
Hin6I GCGC 1 cut(s) 569
HinP1I GCGC 1 cut(s) 569
HincII GTYRAC 1 cut(s) 309
HindII GTYRAC 1 cut(s) 309
HinfI GANTC 7 cut(s) 5, 11, 474, 840, 1042, 1228, 1235
HpaII CCGG 2 cut(s) 85, 144
HphI GGTGA 3 cut(s) 149, 611, 1097
Hpy166II GTNNAC 5 cut(s) 157, 309, 436, 619, 661
Hpy188I TCNGA 6 cut(s) 10, 51, 479, 918, 973, 1015
Hpy188III TCNNGA 8 cut(s) 73, 131, 364, 383, 691, 737, 767, 1232
Hpy8I GTNNAC 5 cut(s) 157, 309, 436, 619, 661
HpyAV CCTTC 4 cut(s) 128, 203, 716, 1050
HpyCH4III ACNGT 3 cut(s) 338, 498, 535
HpyCH4IV ACGT 4 cut(s) 389, 628, 796, 805
HpyCH4V TGCA 9 cut(s) 266, 349, 415, 467, 521, 565, 854, 988, 1169
HpyF10VI GCNNNNNNNGC 6 cut(s) 52, 377, 518, 577, 757, 1166
HpyF3I CTNAG 3 cut(s) 222, 303, 1278
HpySE526I ACGT 4 cut(s) 389, 628, 796, 805
Hsp92II CATG 4 cut(s) 315, 332, 386, 569
HspAI GCGC 1 cut(s) 569
Ksp22I TGATCA 1 cut(s) 976
Kzo9I GATC 3 cut(s) 763, 814, 976
LguI GCTCTTC 2 cut(s) 70, 632
LmnI GCTCC 1 cut(s) 1273
Lsp1109I GCAGC 9 cut(s) 20, 278, 309, 361, 505, 533, 838, 860, 915
LweI GCATC 1 cut(s) 454
MaeI CTAG 1 cut(s) 900
MaeII ACGT 4 cut(s) 389, 628, 796, 805
MaeIII GTNAC 2 cut(s) 184, 1131
MalI GATC 3 cut(s) 765, 816, 978
MboI GATC 3 cut(s) 763, 814, 976
MboII GAAGA 8 cut(s) 57, 335, 649, 666, 728, 731, 800, 815
MfeI CAATTG 1 cut(s) 426
MhlI GDGCHC 2 cut(s) 395, 1215
MluCI AATT 7 cut(s) 230, 426, 603, 791, 860, 1251, 1260
MlyI GAGTC 2 cut(s) 1222, 1244
MmeI TCCRAC 1 cut(s) 484
MnlI CCTC 5 cut(s) 45, 51, 169, 485, 933
MroXI GAANNNNTTC 1 cut(s) 967
MseI TTAA 7 cut(s) 198, 546, 594, 681, 708, 1065, 1197
MslI CAYNNNNRTG 1 cut(s) 993
MspA1I CMGCKG 1 cut(s) 851
MspI CCGG 2 cut(s) 85, 144
MspR9I CCNGG 2 cut(s) 29, 85
MunI CAATTG 1 cut(s) 426
Mva1269I GAATGC 1 cut(s) 373
MvaI CCWGG 1 cut(s) 29
MvnI CGCG 3 cut(s) 820, 1094, 1173
MwoI GCNNNNNNNGC 6 cut(s) 52, 377, 518, 577, 757, 1166
NciI CCSGG 1 cut(s) 85
NdeI CATATG 2 cut(s) 164, 1006
NdeII GATC 3 cut(s) 763, 814, 976
NlaIII CATG 4 cut(s) 315, 332, 386, 569
NlaIV GGNNCC 1 cut(s) 423
NsbI TGCGCA 1 cut(s) 570
NspI RCATGY 2 cut(s) 315, 569
PaeI GCATGC 1 cut(s) 569
PagI TCATGA 1 cut(s) 382
PciSI GCTCTTC 2 cut(s) 70, 632
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 802
PctI GAATGC 1 cut(s) 373
PdmI GAANNNNTTC 1 cut(s) 967
PfeI GAWTC 5 cut(s) 5, 11, 474, 840, 1042
PkrI GCNGC 9 cut(s) 35, 268, 324, 351, 520, 523, 850, 853, 905
PleI GAGTC 2 cut(s) 1222, 1243
PpsI GAGTC 2 cut(s) 1222, 1243
Ppu21I YACGTR 3 cut(s) 390, 629, 797
Psp124BI GAGCTC 1 cut(s) 1215
Psp6I CCWGG 1 cut(s) 27
PspFI CCCAGC 1 cut(s) 59
PspGI CCWGG 1 cut(s) 27
PspN4I GGNNCC 1 cut(s) 423
PspPI GGNCC 1 cut(s) 1087
PstI CTGCAG 2 cut(s) 351, 523
PvuII CAGCTG 1 cut(s) 851
RsaI GTAC 1 cut(s) 627
RsaNI GTAC 1 cut(s) 626
RseI CAYNNNNRTG 1 cut(s) 993
SacI GAGCTC 1 cut(s) 1215
SapI GCTCTTC 2 cut(s) 70, 632
SaqAI TTAA 7 cut(s) 198, 546, 594, 681, 708, 1065, 1197
SatI GCNGC 9 cut(s) 34, 267, 323, 350, 519, 522, 849, 852, 904
Sau3AI GATC 3 cut(s) 763, 814, 976
Sau96I GGNCC 1 cut(s) 1087
SchI GAGTC 2 cut(s) 1222, 1244
ScrFI CCNGG 2 cut(s) 29, 85
SduI GDGCHC 2 cut(s) 395, 1215
SfaNI GCATC 1 cut(s) 454
SfcI CTRYAG 3 cut(s) 347, 519, 876
SinI GGWCC 1 cut(s) 1087
SmiMI CAYNNNNRTG 1 cut(s) 993
SmlI CTYRAG 2 cut(s) 71, 1230
SmoI CTYRAG 2 cut(s) 71, 1230
SphI GCATGC 1 cut(s) 569
Sse9I AATT 7 cut(s) 230, 426, 603, 791, 860, 1251, 1260
SsiI CCGC 3 cut(s) 818, 1108, 1173
SspI AATATT 1 cut(s) 409
SspMI CTAG 1 cut(s) 900
SstI GAGCTC 1 cut(s) 1215
StyD4I CCNGG 2 cut(s) 27, 83
StyI CCWWGG 2 cut(s) 827, 869
TaaI ACNGT 3 cut(s) 338, 498, 535
TaiI ACGT 4 cut(s) 392, 631, 799, 808
TaqI TCGA 2 cut(s) 217, 652
TasI AATT 7 cut(s) 230, 426, 603, 791, 860, 1251, 1260
TfiI GAWTC 5 cut(s) 5, 11, 474, 840, 1042
Tru1I TTAA 7 cut(s) 198, 546, 594, 681, 708, 1065, 1197
Tru9I TTAA 7 cut(s) 198, 546, 594, 681, 708, 1065, 1197
TscAI CASTG 2 cut(s) 21, 341
TseI GCWGC 9 cut(s) 33, 266, 322, 349, 518, 521, 848, 851, 903
TspDTI ATGAA 5 cut(s) 151, 222, 345, 693, 1212
TspGWI ACGGA 1 cut(s) 745
TspRI CASTG 2 cut(s) 21, 341
VpaK11BI GGWCC 1 cut(s) 1087
XapI RAATTY 1 cut(s) 860
XceI RCATGY 2 cut(s) 315, 569
XcmI CCANNNNNNNNNTGG 1 cut(s) 360
XmnI GAANNNNTTC 1 cut(s) 967
XspI CTAG 1 cut(s) 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.