MD01G1058600.v1.1

Acyl-coenzyme A thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003309
Physical Location & Seq
Reverse (-)
16283332 .. 16289801
6470 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1058600.v1.1.491

Sequence Viewer

Length: 1287 bp
ATGGACTCGGAATCAGTAATTGAGTTCCTGGGCTGCGTTCCTCTGCTTCAGAGGCTCCCGAGCTCCTCTCTCAAGAAAATCGCCCAGCTCGTCGTTGTCAAACGCTACGAGAGTGGAGAGTATGTTGTTCGTGAAGGTGAAGCAGGCGACGGGGTTTACTTCATATGGGAAGGAGAGGCTGAAGTTACTGGTTCTGTTAATGCCTATGAAGGAAATCAACCCGAGTTCAAATTGAAACGATATGATTATTTTGGTTATGGTTTTGCATCAATTGAACATCAGGCCGATGTGATTGCTTTATCTAAGCTGACTTGCTTGGTGCTACCCCATGAGAACTGTACTCTGCTGCAGCCAAAATCAATCTGGAATGCTGATGAAGTTCAAGAGACATGCTCTCTTGTTGAGAATATACTGCATTTGGATCCAATTGAAGTAAACATATTTCGAGGGATTACTTTGCCGGATGCACCAAGATTCGGACAGGTTTTTGGAGGACAGTTAGTTGGACAGGCATTGGCTGCGGCAACGAAAACTGTTGATTGTCTTAAACTTGTCCATAGTCTGCATGCACATTTTCTGCTTGTTGGGGATTGTAACACACCAATTATATATCAAGTCCACAGAGTACGTGATGGGAAGAGCTTTGCTACTCGAAGAGTGGATGCAATACAAAAGGGGATTAACATTTTCACGTTATTGGCATCATTTAAAAAAGAAGAAGAAGGGTCTGTCCACCAGCAAGCAACAATGCTATCTGTGCCTGCTCCAGATAAACTTCTGTCAATGGAAGAACTACGTGAAAAACGTCTTCTTGATCCACGTCTTCCAAGGACTTATCGAATCAAGGCAGCTGCAAAGAAATTTGTGCCTTGGCCTATAGAGATACGATTTTGTGAGCCTAGCAGCTCAACCAATCAGACTAAATCTGCACCGAGTTTGAGGTTCTGGTTTAGAGCAAAAGGAAAACTTTCTGACGATCAGGCTTTGCATAGATGTGTTGTAGCATATGCTTCAGATTTGATATTTGCTGCTGTAAGTCTGAATCCCCACCGCAGAATTGGAGTTAAGTTGAGTCAAGTTAGTTTGGACCATGCGATGTGGTTTCACCGTCATGTTAGAGCTGATGACTGGTTACTGTATGTGATAGTGAGTCCGATTGCCTACGACACACGTGGATTTGTTTCAGGCCAAATGTTTAACAGAGAGGGAGAGCTTATTGTATCATTGACCCAAGAGTCCCTAAACAGGCCAATCAGACCAACTCCGGCTGCCACATCTAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0006082 GO:0006139 GO:0006163 GO:0006629 GO:0006631 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009117 GO:0009150 GO:0009259 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010559 GO:0010561 GO:0010605 GO:0016042 GO:0016043 GO:0016054 GO:0016289 GO:0016290 GO:0016559 GO:0016787 GO:0016788 GO:0016790 GO:0019222 GO:0019637 GO:0019693 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031907 GO:0031974 GO:0032787 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043648 GO:0043649 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045223 GO:0045225 GO:0046395 GO:0046483 GO:0047617 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051186 GO:0051246 GO:0051248 GO:0052815 GO:0055086 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072521 GO:0080090 GO:1901135 GO:1901360 GO:1901564 GO:1901575 GO:1903018 GO:1903019 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01100 ko01110 map00062 map01040 map01100 map01110
Pfam Domains
Protein Families
4HBT_3 Acyl_CoA_thio cNMP_binding

Protein Analysis

429

Amino Acids

48.08

Weight (kDa)

8.05

Isoelectric Point (pI)

34.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cNMP_binding PF00027 34 - 112 3.8e-10 Cyclic nucleotide-binding domain
4HBT_3 PF13622 157 - 236 1.7e-17 Acyl-CoA thioesterase N-terminal domain
Acyl_CoA_thio PF02551 301 - 412 3.9e-26 Acyl-CoA thioesterase
4HBT_3C PF20789 303 - 413 1.7e-21 Acyl-CoA thioesterase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1234
AciI CCGC 2 cut(s) 521, 1051
AclWI GGATC 3 cut(s) 416, 429, 809
AcsI RAATTY 1 cut(s) 860
AcuI CTGAAG 3 cut(s) 32, 201, 996
AcvI CACGTG 1 cut(s) 1172
AfaI GTAC 2 cut(s) 340, 627
AfiI CCNNNNNNNGG 2 cut(s) 476, 1245
AflIII ACRYGT 1 cut(s) 1169
AgsI TTSAA 5 cut(s) 229, 235, 275, 383, 431
AjiI CACGTC 1 cut(s) 821
AjnI CCWGG 1 cut(s) 27
AluBI AGCT 8 cut(s) 63, 88, 307, 642, 851, 906, 1121, 1213
AluI AGCT 8 cut(s) 63, 88, 307, 642, 851, 906, 1121, 1213
Alw21I GWGCWC 1 cut(s) 65
Alw26I GTCTC 1 cut(s) 380
AlwI GGATC 3 cut(s) 416, 429, 809
Ama87I CYCGRG 2 cut(s) 58, 221
AoxI GGCC 4 cut(s) 282, 872, 1186, 1247
ApeKI GCWGC 9 cut(s) 33, 346, 349, 518, 848, 851, 903, 1028, 1268
ApoI RAATTY 1 cut(s) 860
Asp700I GAANNNNTTC 1 cut(s) 967
AspS9I GGNCC 1 cut(s) 1087
AsuHPI GGTGA 2 cut(s) 149, 1097
AvaI CYCGRG 2 cut(s) 58, 221
AvaII GGWCC 1 cut(s) 1087
BamHI GGATCC 1 cut(s) 421
BanII GRGCYC 1 cut(s) 65
BbrPI CACGTG 1 cut(s) 1172
BbsI GAAGAC 2 cut(s) 800, 815
Bbv12I GWGCWC 1 cut(s) 65
BbvI GCAGC 9 cut(s) 20, 333, 361, 505, 838, 860, 915, 1015, 1255
BccI CCATC 1 cut(s) 626
BcgI CGANNNNNNTGC 2 cut(s) 275, 309
BciT130I CCWGG 1 cut(s) 29
BcoDI GTCTC 1 cut(s) 380
BfaI CTAG 1 cut(s) 900
BfmI CTRYAG 2 cut(s) 347, 876
Bme1390I CCNGG 1 cut(s) 29
Bme18I GGWCC 1 cut(s) 1087
BmeT110I CYCGRG 2 cut(s) 58, 221
BmgBI CACGTC 1 cut(s) 821
BmgT120I GGNCC 1 cut(s) 1087
BmiI GGNNCC 2 cut(s) 56, 423
BmrFI CCNGG 1 cut(s) 29
BmsI GCATC 4 cut(s) 275, 454, 652, 710
BpiI GAAGAC 2 cut(s) 800, 815
BplI GAGNNNNNCTC 4 cut(s) 52, 84, 377, 409
BpmI CTGGAG 1 cut(s) 750
BpuEI CTTGAG 1 cut(s) 56
BsaAI YACGTR 3 cut(s) 629, 797, 1172
BsaJI CCNNGG 3 cut(s) 28, 827, 869
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 2 cut(s) 476, 1245
Bse1I ACTGG 2 cut(s) 193, 1133
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 3 cut(s) 28, 827, 869
BseGI GGATG 2 cut(s) 469, 667
BseLI CCNNNNNNNGG 2 cut(s) 476, 1245
BseNI ACTGG 2 cut(s) 193, 1133
BseRI GAGGAG 1 cut(s) 55
BseXI GCAGC 9 cut(s) 20, 333, 361, 505, 838, 860, 915, 1015, 1255
BseYI CCCAGC 1 cut(s) 84
BsgI GTGCAG 1 cut(s) 912
BshFI GGCC 4 cut(s) 284, 874, 1188, 1249
BsiHKAI GWGCWC 1 cut(s) 65
BsiHKCI CYCGRG 2 cut(s) 58, 221
BsiSI CCGG 2 cut(s) 461, 1265
BslFI GGGAC 1 cut(s) 1222
BslI CCNNNNNNNGG 2 cut(s) 476, 1245
BsmAI GTCTC 1 cut(s) 380
BsmFI GGGAC 1 cut(s) 1222
BsmI GAATGC 1 cut(s) 373
BsnI GGCC 4 cut(s) 284, 874, 1188, 1249
BsoBI CYCGRG 2 cut(s) 58, 221
Bsp1286I GDGCHC 1 cut(s) 65
Bsp143I GATC 3 cut(s) 421, 814, 976
BspACI CCGC 2 cut(s) 521, 1051
BspANI GGCC 4 cut(s) 284, 874, 1188, 1249
BspLI GGNNCC 2 cut(s) 56, 423
BspMAI CTGCAG 1 cut(s) 351
BspPI GGATC 3 cut(s) 416, 429, 809
BspQI GCTCTTC 1 cut(s) 632
BsrI ACTGG 2 cut(s) 193, 1133
BssECI CCNNGG 3 cut(s) 28, 827, 869
BssMI GATC 3 cut(s) 421, 814, 976
BssT1I CCWWGG 2 cut(s) 827, 869
Bst2UI CCWGG 1 cut(s) 29
Bst4CI ACNGT 5 cut(s) 338, 498, 535, 1109, 1137
Bst6I CTCTTC 2 cut(s) 632, 649
BstAPI GCANNNNNTGC 1 cut(s) 518
BstBAI YACGTR 3 cut(s) 629, 797, 1172
BstC8I GCNNGC 4 cut(s) 145, 567, 741, 762
BstDEI CTNAG 2 cut(s) 303, 1278
BstF5I GGATG 2 cut(s) 469, 667
BstKTI GATC 3 cut(s) 424, 817, 979
BstMAI GTCTC 1 cut(s) 380
BstMBI GATC 3 cut(s) 421, 814, 976
BstMWI GCNNNNNNNGC 3 cut(s) 52, 518, 757
BstNI CCWGG 1 cut(s) 29
BstNSI RCATGY 2 cut(s) 393, 569
BstSCI CCNGG 1 cut(s) 27
BstSFI CTRYAG 2 cut(s) 347, 876
BstV1I GCAGC 9 cut(s) 20, 333, 361, 505, 838, 860, 915, 1015, 1255
BstV2I GAAGAC 2 cut(s) 800, 815
BstX2I RGATCY 1 cut(s) 421
BstYI RGATCY 1 cut(s) 421
BsuRI GGCC 4 cut(s) 284, 874, 1188, 1249
BtgZI GCGATG 1 cut(s) 1109
BtrI CACGTC 1 cut(s) 821
BtsCI GGATG 2 cut(s) 469, 667
Cac8I GCNNGC 4 cut(s) 145, 567, 741, 762
Cfr13I GGNCC 1 cut(s) 1087
Csp6I GTAC 2 cut(s) 339, 626
CviAII CATG 5 cut(s) 329, 390, 566, 1091, 1112
CviQI GTAC 2 cut(s) 339, 626
DdeI CTNAG 2 cut(s) 303, 1278
DpnI GATC 3 cut(s) 423, 816, 978
DpnII GATC 3 cut(s) 421, 814, 976
DraI TTTAAA 1 cut(s) 709
DrdI GACNNNNNNGTC 1 cut(s) 1234
DseDI GACNNNNNNGTC 1 cut(s) 1234
Eam1104I CTCTTC 2 cut(s) 632, 649
EarI CTCTTC 2 cut(s) 632, 649
Ecl136II GAGCTC 1 cut(s) 63
Eco130I CCWWGG 2 cut(s) 827, 869
Eco24I GRGCYC 1 cut(s) 65
Eco47I GGWCC 1 cut(s) 1087
Eco53kI GAGCTC 1 cut(s) 63
Eco57I CTGAAG 3 cut(s) 32, 201, 996
Eco72I CACGTG 1 cut(s) 1172
Eco88I CYCGRG 2 cut(s) 58, 221
EcoICRI GAGCTC 1 cut(s) 63
EcoRII CCWGG 1 cut(s) 27
EcoT14I CCWWGG 2 cut(s) 827, 869
EcoT38I GRGCYC 1 cut(s) 65
ErhI CCWWGG 2 cut(s) 827, 869
FaeI CATG 5 cut(s) 332, 393, 569, 1094, 1115
FalI AAGNNNNNCTT 2 cut(s) 951, 983
FaqI GGGAC 1 cut(s) 1222
FatI CATG 5 cut(s) 328, 389, 565, 1090, 1111
FauNDI CATATG 2 cut(s) 164, 1006
FokI GGATG 2 cut(s) 476, 674
FriOI GRGCYC 1 cut(s) 65
FspBI CTAG 1 cut(s) 900
GsaI CCCAGC 1 cut(s) 88
GsuI CTGGAG 1 cut(s) 750
HaeIII GGCC 4 cut(s) 284, 874, 1188, 1249
HapII CCGG 2 cut(s) 461, 1265
Hin1II CATG 5 cut(s) 332, 393, 569, 1094, 1115
HinfI GANTC 8 cut(s) 5, 11, 474, 840, 1042, 1072, 1150, 1235
HpaII CCGG 2 cut(s) 461, 1265
HphI GGTGA 2 cut(s) 149, 1097
Hpy166II GTNNAC 4 cut(s) 157, 436, 619, 733
Hpy188I TCNGA 9 cut(s) 10, 51, 479, 918, 973, 1015, 1041, 1155, 1256
Hpy188III TCNNGA 7 cut(s) 58, 73, 131, 364, 383, 767, 812
Hpy8I GTNNAC 4 cut(s) 157, 436, 619, 733
Hpy99I CGWCG 2 cut(s) 95, 152
HpyAV CCTTC 4 cut(s) 128, 164, 203, 716
HpyCH4III ACNGT 5 cut(s) 338, 498, 535, 1109, 1137
HpyCH4IV ACGT 6 cut(s) 628, 692, 796, 805, 820, 1171
HpyF10VI GCNNNNNNNGC 3 cut(s) 52, 518, 757
HpyF3I CTNAG 2 cut(s) 303, 1278
HpySE526I ACGT 6 cut(s) 628, 692, 796, 805, 820, 1171
Hsp92II CATG 5 cut(s) 332, 393, 569, 1094, 1115
Kzo9I GATC 3 cut(s) 421, 814, 976
LguI GCTCTTC 1 cut(s) 632
LmnI GCTCC 3 cut(s) 60, 68, 769
Lsp1109I GCAGC 9 cut(s) 20, 333, 361, 505, 838, 860, 915, 1015, 1255
LweI GCATC 4 cut(s) 275, 454, 652, 710
MaeI CTAG 1 cut(s) 900
MaeII ACGT 6 cut(s) 628, 692, 796, 805, 820, 1171
MaeIII GTNAC 3 cut(s) 184, 593, 1131
MalI GATC 3 cut(s) 423, 816, 978
MboI GATC 3 cut(s) 421, 814, 976
MboII GAAGA 7 cut(s) 649, 666, 728, 731, 800, 800, 815
MfeI CAATTG 2 cut(s) 270, 426
MflI RGATCY 1 cut(s) 421
MhlI GDGCHC 1 cut(s) 65
MluCI AATT 7 cut(s) 18, 230, 270, 426, 603, 860, 1056
MlyI GAGTC 3 cut(s) 1081, 1159, 1244
MmeI TCCRAC 1 cut(s) 484
MnlI CCTC 8 cut(s) 45, 51, 76, 169, 440, 485, 933, 1198
MroXI GAANNNNTTC 1 cut(s) 967
MseI TTAA 6 cut(s) 198, 546, 681, 708, 1065, 1197
MslI CAYNNNNRTG 2 cut(s) 993, 1110
MspA1I CMGCKG 1 cut(s) 851
MspI CCGG 2 cut(s) 461, 1265
MspR9I CCNGG 1 cut(s) 29
MunI CAATTG 2 cut(s) 270, 426
Mva1269I GAATGC 1 cut(s) 373
MvaI CCWGG 1 cut(s) 29
MwoI GCNNNNNNNGC 3 cut(s) 52, 518, 757
NdeI CATATG 2 cut(s) 164, 1006
NdeII GATC 3 cut(s) 421, 814, 976
NlaIII CATG 5 cut(s) 332, 393, 569, 1094, 1115
NlaIV GGNNCC 2 cut(s) 56, 423
NspI RCATGY 2 cut(s) 393, 569
PaeI GCATGC 1 cut(s) 569
PciSI GCTCTTC 1 cut(s) 632
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 802
PctI GAATGC 1 cut(s) 373
PdmI GAANNNNTTC 1 cut(s) 967
PfeI GAWTC 4 cut(s) 11, 474, 840, 1042
PleI GAGTC 3 cut(s) 1080, 1158, 1243
PmaCI CACGTG 1 cut(s) 1172
PmlI CACGTG 1 cut(s) 1172
PpsI GAGTC 3 cut(s) 1080, 1158, 1243
Ppu21I YACGTR 3 cut(s) 629, 797, 1172
Psp124BI GAGCTC 1 cut(s) 65
Psp6I CCWGG 1 cut(s) 27
PspCI CACGTG 1 cut(s) 1172
PspFI CCCAGC 1 cut(s) 84
PspGI CCWGG 1 cut(s) 27
PspN4I GGNNCC 2 cut(s) 56, 423
PspPI GGNCC 1 cut(s) 1087
PstI CTGCAG 1 cut(s) 351
PsuI RGATCY 1 cut(s) 421
PvuII CAGCTG 1 cut(s) 851
RsaI GTAC 2 cut(s) 340, 627
RsaNI GTAC 2 cut(s) 339, 626
RseI CAYNNNNRTG 2 cut(s) 993, 1110
SacI GAGCTC 1 cut(s) 65
SapI GCTCTTC 1 cut(s) 632
SaqAI TTAA 6 cut(s) 198, 546, 681, 708, 1065, 1197
Sau3AI GATC 3 cut(s) 421, 814, 976
Sau96I GGNCC 1 cut(s) 1087
SchI GAGTC 3 cut(s) 1081, 1159, 1244
ScrFI CCNGG 1 cut(s) 29
SduI GDGCHC 1 cut(s) 65
SfaNI GCATC 4 cut(s) 275, 454, 652, 710
SfcI CTRYAG 2 cut(s) 347, 876
SinI GGWCC 1 cut(s) 1087
SmiMI CAYNNNNRTG 2 cut(s) 993, 1110
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
SphI GCATGC 1 cut(s) 569
Sse9I AATT 7 cut(s) 18, 230, 270, 426, 603, 860, 1056
SsiI CCGC 2 cut(s) 521, 1051
SspMI CTAG 1 cut(s) 900
SstI GAGCTC 1 cut(s) 65
StyD4I CCNGG 1 cut(s) 27
StyI CCWWGG 2 cut(s) 827, 869
TaaI ACNGT 5 cut(s) 338, 498, 535, 1109, 1137
TaiI ACGT 6 cut(s) 631, 695, 799, 808, 823, 1174
TaqI TCGA 3 cut(s) 445, 652, 838
TasI AATT 7 cut(s) 18, 230, 270, 426, 603, 860, 1056
TatI WGTACW 1 cut(s) 338
TauI GCSGC 1 cut(s) 524
TfiI GAWTC 4 cut(s) 11, 474, 840, 1042
Tru1I TTAA 6 cut(s) 198, 546, 681, 708, 1065, 1197
Tru9I TTAA 6 cut(s) 198, 546, 681, 708, 1065, 1197
TseI GCWGC 9 cut(s) 33, 346, 349, 518, 848, 851, 903, 1028, 1268
TspDTI ATGAA 3 cut(s) 151, 222, 390
VpaK11BI GGWCC 1 cut(s) 1087
XapI RAATTY 1 cut(s) 860
XceI RCATGY 2 cut(s) 393, 569
XcmI CCANNNNNNNNNTGG 2 cut(s) 360, 1055
XmnI GAANNNNTTC 1 cut(s) 967
XspI CTAG 1 cut(s) 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.