MD01G1058500.v1.1

Acyl-coenzyme A thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003309
Physical Location & Seq
Reverse (-)
16268711 .. 16270298
1588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1058500.v1.1.491

Sequence Viewer

Length: 513 bp
ATGGACTCGGAATCAGTAATTGAGTTCCTGGGCCGCGTTCCTCTGCTTCAGAGGCTCCCCAGCTCCTCTCTCAAGAGAATCGCCCAGCTCGTCATTTTTAAACACTACGAGAGTGGAGAGTATGTTGTTCGCGAAGGCGAAACAGGGGACGTGGTTTACTTCATATGGGAAGGAGAGGCTGAAGTTATTGGTTCTGTTAATGCTGATGAAGGAAATCGACCTGAGTTCAAATTGAAACGATATGATTATTTTGGTTATGGTTTTGCATCAATTGAGCATCAGGCCGATGTGATTGCTTTATCTAAGCTGACTTGCTTGGTGCTACCCCATGGGAATTTTTCTCTGCTGCAGCCAAAATCAATCTGGAACGCTGATGAAGCTCAAGAGACATGCTCTCTTGTTGAGAATATATTACATTTGGATCCAAGTGAAGTATTTATCTACCAAATCTTCAAGAGAATACGTTCTCTGTCCTTTGTTTTATTTGGTTATAATGATGTTGAAGAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0006082 GO:0006139 GO:0006163 GO:0006629 GO:0006631 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009117 GO:0009150 GO:0009259 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010559 GO:0010561 GO:0010605 GO:0016042 GO:0016043 GO:0016054 GO:0016289 GO:0016290 GO:0016559 GO:0016787 GO:0016788 GO:0016790 GO:0019222 GO:0019637 GO:0019693 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031907 GO:0031974 GO:0032787 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043648 GO:0043649 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045223 GO:0045225 GO:0046395 GO:0046483 GO:0047617 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051186 GO:0051246 GO:0051248 GO:0052815 GO:0055086 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072521 GO:0080090 GO:1901135 GO:1901360 GO:1901564 GO:1901575 GO:1903018 GO:1903019 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01100 ko01110 map00062 map01040 map01100 map01110
Pfam Domains
Protein Families
4HBT_3 Acyl_CoA_thio cNMP_binding

Protein Analysis

171

Amino Acids

19.43

Weight (kDa)

4.76

Isoelectric Point (pI)

44.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cNMP_binding PF00027 34 - 113 1.1e-10 Cyclic nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 492
AccII CGCG 2 cut(s) 36, 132
AciI CCGC 1 cut(s) 34
AclWI GGATC 2 cut(s) 416, 429
AcsI RAATTY 1 cut(s) 334
AcuI CTGAAG 2 cut(s) 32, 201
AgsI TTSAA 4 cut(s) 229, 235, 454, 503
AjiI CACGTC 1 cut(s) 151
AjnI CCWGG 1 cut(s) 27
AluBI AGCT 4 cut(s) 63, 88, 307, 380
AluI AGCT 4 cut(s) 63, 88, 307, 380
Alw26I GTCTC 1 cut(s) 380
AlwI GGATC 2 cut(s) 416, 429
AoxI GGCC 2 cut(s) 31, 282
ApeKI GCWGC 2 cut(s) 346, 349
ApoI RAATTY 1 cut(s) 334
Asp700I GAANNNNTTC 1 cut(s) 463
AspS9I GGNCC 1 cut(s) 31
BamHI GGATCC 1 cut(s) 421
BbvI GCAGC 2 cut(s) 333, 361
BcgI CGANNNNNNTGC 2 cut(s) 275, 309
BciT130I CCWGG 1 cut(s) 29
BcoDI GTCTC 1 cut(s) 380
BfmI CTRYAG 1 cut(s) 347
BisI GCNGC 3 cut(s) 34, 347, 350
BlsI GCNGC 3 cut(s) 35, 348, 351
Bme1390I CCNGG 1 cut(s) 29
BmgBI CACGTC 1 cut(s) 151
BmgT120I GGNCC 1 cut(s) 31
BmiI GGNNCC 2 cut(s) 56, 423
BmrFI CCNGG 1 cut(s) 29
BmsI GCATC 2 cut(s) 275, 286
BplI GAGNNNNNCTC 2 cut(s) 377, 409
BpuEI CTTGAG 2 cut(s) 56, 366
BsaJI CCNNGG 2 cut(s) 28, 328
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 2 cut(s) 28, 328
BseMII CTCAG 1 cut(s) 213
BseRI GAGGAG 1 cut(s) 55
BseXI GCAGC 2 cut(s) 333, 361
BseYI CCCAGC 2 cut(s) 59, 84
Bsh1236I CGCG 2 cut(s) 36, 132
BshFI GGCC 2 cut(s) 33, 284
BslFI GGGAC 1 cut(s) 161
BsmAI GTCTC 1 cut(s) 380
BsmFI GGGAC 1 cut(s) 161
BsnI GGCC 2 cut(s) 33, 284
Bsp143I GATC 1 cut(s) 421
Bsp19I CCATGG 1 cut(s) 328
Bsp68I TCGCGA 1 cut(s) 132
BspACI CCGC 1 cut(s) 34
BspANI GGCC 2 cut(s) 33, 284
BspCNI CTCAG 1 cut(s) 214
BspFNI CGCG 2 cut(s) 36, 132
BspLI GGNNCC 2 cut(s) 56, 423
BspMAI CTGCAG 1 cut(s) 351
BspPI GGATC 2 cut(s) 416, 429
BssECI CCNNGG 2 cut(s) 28, 328
BssMI GATC 1 cut(s) 421
BssT1I CCWWGG 1 cut(s) 328
Bst2UI CCWGG 1 cut(s) 29
Bst6I CTCTTC 1 cut(s) 498
BstDEI CTNAG 2 cut(s) 222, 303
BstDSI CCRYGG 1 cut(s) 328
BstFNI CGCG 2 cut(s) 36, 132
BstKTI GATC 1 cut(s) 424
BstMAI GTCTC 1 cut(s) 380
BstMBI GATC 1 cut(s) 421
BstMWI GCNNNNNNNGC 2 cut(s) 52, 377
BstNI CCWGG 1 cut(s) 29
BstNSI RCATGY 1 cut(s) 393
BstSCI CCNGG 1 cut(s) 27
BstSFI CTRYAG 1 cut(s) 347
BstUI CGCG 2 cut(s) 36, 132
BstV1I GCAGC 2 cut(s) 333, 361
BstX2I RGATCY 1 cut(s) 421
BstYI RGATCY 1 cut(s) 421
BsuRI GGCC 2 cut(s) 33, 284
BtgI CCRYGG 1 cut(s) 328
BtrI CACGTC 1 cut(s) 151
BtuMI TCGCGA 1 cut(s) 132
Cfr13I GGNCC 1 cut(s) 31
CviAII CATG 2 cut(s) 329, 390
CviJI RGCY 9 cut(s) 33, 55, 63, 88, 179, 284, 307, 352, 380
CviKI_1 RGCY 9 cut(s) 33, 55, 63, 88, 179, 284, 307, 352, 380
DdeI CTNAG 2 cut(s) 222, 303
DpnI GATC 1 cut(s) 423
DpnII GATC 1 cut(s) 421
DraI TTTAAA 1 cut(s) 100
Eam1104I CTCTTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 498
Eco130I CCWWGG 1 cut(s) 328
Eco57I CTGAAG 2 cut(s) 32, 201
EcoRII CCWGG 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 328
ErhI CCWWGG 1 cut(s) 328
FaeI CATG 2 cut(s) 332, 393
FaiI YATR 9 cut(s) 123, 164, 166, 243, 258, 330, 391, 410, 492
FaqI GGGAC 1 cut(s) 161
FatI CATG 2 cut(s) 328, 389
FauNDI CATATG 1 cut(s) 164
Fnu4HI GCNGC 3 cut(s) 34, 347, 350
Fsp4HI GCNGC 3 cut(s) 34, 347, 350
GluI GCNGC 3 cut(s) 34, 347, 350
GsaI CCCAGC 2 cut(s) 63, 88
HaeIII GGCC 2 cut(s) 33, 284
Hin1II CATG 2 cut(s) 332, 393
HinfI GANTC 3 cut(s) 5, 11, 78
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 2 cut(s) 10, 51
Hpy188III TCNNGA 5 cut(s) 73, 131, 364, 383, 454
Hpy8I GTNNAC 1 cut(s) 157
HpyAV CCTTC 3 cut(s) 128, 164, 203
HpyCH4IV ACGT 2 cut(s) 150, 463
HpyCH4V TGCA 2 cut(s) 266, 349
HpyF10VI GCNNNNNNNGC 2 cut(s) 52, 377
HpyF3I CTNAG 2 cut(s) 222, 303
HpySE526I ACGT 2 cut(s) 150, 463
Hsp92II CATG 2 cut(s) 332, 393
Kzo9I GATC 1 cut(s) 421
LmnI GCTCC 2 cut(s) 60, 68
LpnPI CCDG 8 cut(s) 14, 41, 73, 98, 129, 234, 266, 349
Lsp1109I GCAGC 2 cut(s) 333, 361
LweI GCATC 2 cut(s) 275, 286
MaeII ACGT 2 cut(s) 150, 463
MalI GATC 1 cut(s) 423
MboI GATC 1 cut(s) 421
MboII GAAGA 1 cut(s) 442
MfeI CAATTG 1 cut(s) 270
MflI RGATCY 1 cut(s) 421
MluCI AATT 4 cut(s) 18, 230, 270, 334
MnlI CCTC 4 cut(s) 45, 51, 76, 169
MroXI GAANNNNTTC 1 cut(s) 463
MseI TTAA 2 cut(s) 99, 198
MspR9I CCNGG 1 cut(s) 29
MunI CAATTG 1 cut(s) 270
MvaI CCWGG 1 cut(s) 29
MvnI CGCG 2 cut(s) 36, 132
MwoI GCNNNNNNNGC 2 cut(s) 52, 377
NcoI CCATGG 1 cut(s) 328
NdeI CATATG 1 cut(s) 164
NdeII GATC 1 cut(s) 421
NlaIII CATG 2 cut(s) 332, 393
NlaIV GGNNCC 2 cut(s) 56, 423
NruI TCGCGA 1 cut(s) 132
NspI RCATGY 1 cut(s) 393
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PdmI GAANNNNTTC 1 cut(s) 463
PfeI GAWTC 2 cut(s) 11, 78
PkrI GCNGC 3 cut(s) 35, 348, 351
PsiI TTATAA 1 cut(s) 492
Psp6I CCWGG 1 cut(s) 27
PspFI CCCAGC 2 cut(s) 59, 84
PspGI CCWGG 1 cut(s) 27
PspN4I GGNNCC 2 cut(s) 56, 423
PspPI GGNCC 1 cut(s) 31
PstI CTGCAG 1 cut(s) 351
PsuI RGATCY 1 cut(s) 421
RruI TCGCGA 1 cut(s) 132
SaqAI TTAA 2 cut(s) 99, 198
SatI GCNGC 3 cut(s) 34, 347, 350
Sau3AI GATC 1 cut(s) 421
Sau96I GGNCC 1 cut(s) 31
ScrFI CCNGG 1 cut(s) 29
SetI ASST 7 cut(s) 65, 90, 153, 223, 309, 382, 466
SfaNI GCATC 2 cut(s) 275, 286
SfcI CTRYAG 1 cut(s) 347
SmlI CTYRAG 2 cut(s) 71, 381
SmoI CTYRAG 2 cut(s) 71, 381
Sse9I AATT 4 cut(s) 18, 230, 270, 334
SsiI CCGC 1 cut(s) 34
StyD4I CCNGG 1 cut(s) 27
StyI CCWWGG 1 cut(s) 328
TaiI ACGT 2 cut(s) 153, 466
TaqI TCGA 1 cut(s) 217
TasI AATT 4 cut(s) 18, 230, 270, 334
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 2 cut(s) 11, 78
Tru1I TTAA 2 cut(s) 99, 198
Tru9I TTAA 2 cut(s) 99, 198
TseI GCWGC 2 cut(s) 346, 349
TspDTI ATGAA 3 cut(s) 151, 222, 390
XapI RAATTY 1 cut(s) 334
XceI RCATGY 1 cut(s) 393
XcmI CCANNNNNNNNNTGG 1 cut(s) 360
XmnI GAANNNNTTC 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.