FvH4_7g14192

Acyl-coenzyme A thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003309
Physical Location & Seq
Reverse (-)
12560426 .. 12567085
6660 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g14192.t2

Sequence Viewer

Length: 1287 bp
ATGGACTCCGAATCAGTGATTGAGTTCCTGGGCTGCGTTCCTCTGCTGCAGCGCCTACCCAGCTCTTCCCTCAAGAAAATCGCCCAACTCGTTATTCCAAAGCACTACGATGCAGGAGAGTATGTGGTTCGTGAAGGGGAGGTTGGGATTGGGGTTTACTTCATTTGGGAAGGAGAGGCTGAAGTCACTGGGTCTGTTAATGCTGATGAAGATAACCGGCCGGAGTTTCAGTTGAAGAGATATGATTACTTCGGTTATGGTTTTAAAGCATTAGGTCATCAAGCTGATGTAATTGCTTCATCTAAGCTGACATGCTTGGTGCTACCCCATGAACATTGTTCTCTGCTGCAGCCAAAATCAATCTGGCATGCGGATAAATCTCTGGAGACATGCTCTCTTGTTGAGAATATATTGCATTTGGAGCCAATTGAAGCAAATATATTTCAGGGGATTACTTTACCAGAGGCACCAAGATTTGGACAGGTTTTCGGAGGACAGTTAGTTGGACAGGCATTGGCTGCAGCAACAAAAACTGTTGACTGTCTCAAAATCGTCCACAGTCTGCATGCACATTTTCTGCTTGTTGGGGATTTCAACACACCAATTATATATCAAGTTCACCGAGTACGTGATGGAAAGAGCTTTGTTACTCGAAGAGTAGACGCAATACAAAAAGGGATAAACATATTCACTTTACTGGCATCCTTTCAAAAAGAAGAACAAGGGTCTGTCCACCAGGAAGCCATAATGCCATCTGTTCCTTCCCCAGATACGCTTCCATCAATGGAAGAACTACGGGAAAGACGTCTTTTTGATCCCCGTCTTCCAAGAACTTATCGAATAAAAGTAGCTGCAAAGGAATTTGCCCCTTGGCCAATTGAGATACGATTTTGTGAACCTAGTAGCCGGACCAATCAGACTAAGTCTCCACCAAGTTTGAGGTTCTGGTTTAAAGCAAAAGGAAAACTTTCAGATGATCAGGCTTTGCATAGATGTGTTGTGGCTTATGCTTCAGATTTGATATTCTCTGGTGTAAGTCTAAATCCTCACCGTAAAAGAGGGCATAAAACAAGTCAAGTTAGTTTGGACCATGCAATGTGGTTTCACCGCCCCCTTAGAGCTGATGACTGGTTACTACATGTGATAGTGAGTCCCATTGCCTATAATGCACGCGGCTATGTCACAGGCCAAATGTTCAATACCAAGGGAGAGCTTGTTGTATCATTGACTCAAGAGTCCCTGAACAGGCCAATTAGACCAACTGCGGCTGTCACATCTAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0006082 GO:0006139 GO:0006163 GO:0006629 GO:0006631 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009117 GO:0009150 GO:0009259 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010559 GO:0010561 GO:0010605 GO:0016042 GO:0016043 GO:0016054 GO:0016289 GO:0016290 GO:0016559 GO:0016787 GO:0016788 GO:0016790 GO:0019222 GO:0019637 GO:0019693 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031907 GO:0031974 GO:0032787 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043648 GO:0043649 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045223 GO:0045225 GO:0046395 GO:0046483 GO:0047617 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051186 GO:0051246 GO:0051248 GO:0052815 GO:0055086 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072521 GO:0080090 GO:1901135 GO:1901360 GO:1901564 GO:1901575 GO:1903018 GO:1903019 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01100 ko01110 map00062 map01040 map01100 map01110
Pfam Domains
Protein Families
4HBT_3 Acyl_CoA_thio cNMP_binding

Protein Analysis

429

Amino Acids

48.12

Weight (kDa)

8.37

Isoelectric Point (pI)

38.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HBT_3 PF13622 143 - 222 6.3e-17 Acyl-CoA thioesterase N-terminal domain
4HBT_3C PF20789 287 - 398 2.5e-22 Acyl-CoA thioesterase C-terminal domain
Acyl_CoA_thio PF02551 290 - 397 1.1e-23 Acyl-CoA thioesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1234
AatII GACGTC 1 cut(s) 808
AccB1I GGYRCC 1 cut(s) 466
AccB7I CCANNNNNTGG 1 cut(s) 476
AccI GTMKAC 1 cut(s) 660
AccII CGCG 1 cut(s) 1173
AciI CCGC 4 cut(s) 371, 1108, 1173, 1265
AclWI GGATC 1 cut(s) 809
AcoI YGGCCR 2 cut(s) 218, 872
AcsI RAATTY 1 cut(s) 860
AcuI CTGAAG 2 cut(s) 201, 996
AcyI GRCGYC 1 cut(s) 805
AfaI GTAC 1 cut(s) 627
AfiI CCNNNNNNNGG 2 cut(s) 476, 1245
AflIII ACRYGT 1 cut(s) 1138
AgsI TTSAA 5 cut(s) 235, 431, 595, 710, 1198
AjnI CCWGG 2 cut(s) 27, 735
AjuI GAANNNNNNNTTGG 4 cut(s) 78, 110, 126, 158
AluBI AGCT 7 cut(s) 63, 284, 307, 642, 851, 1121, 1213
AluI AGCT 7 cut(s) 63, 284, 307, 642, 851, 1121, 1213
Alw26I GTCTC 3 cut(s) 380, 548, 930
AlwI GGATC 1 cut(s) 809
AoxI GGCC 4 cut(s) 218, 872, 1186, 1247
ApeKI GCWGC 8 cut(s) 33, 46, 49, 346, 349, 518, 521, 851
ApoI RAATTY 1 cut(s) 860
Asp700I GAANNNNTTC 1 cut(s) 967
AspLEI GCGC 1 cut(s) 54
AspS9I GGNCC 2 cut(s) 909, 1087
AsuHPI GGTGA 3 cut(s) 611, 1040, 1097
AvaII GGWCC 2 cut(s) 909, 1087
BalI TGGCCA 1 cut(s) 874
BanI GGYRCC 1 cut(s) 466
BbsI GAAGAC 1 cut(s) 815
BbvI GCAGC 8 cut(s) 20, 33, 61, 333, 361, 505, 533, 838
BccI CCATC 3 cut(s) 626, 760, 787
BciT130I CCWGG 2 cut(s) 29, 737
BclI TGATCA 1 cut(s) 976
BcoDI GTCTC 3 cut(s) 380, 548, 930
BfaI CTAG 1 cut(s) 900
BfmI CTRYAG 3 cut(s) 47, 347, 519
BfoI RGCGCY 1 cut(s) 55
Bme1390I CCNGG 2 cut(s) 29, 737
Bme18I GGWCC 2 cut(s) 909, 1087
BmgT120I GGNCC 2 cut(s) 909, 1087
BmiI GGNNCC 2 cut(s) 423, 468
BmrFI CCNGG 2 cut(s) 29, 737
BmrI ACTGGG 1 cut(s) 198
BmsI GCATC 2 cut(s) 100, 710
BmuI ACTGGG 1 cut(s) 198
BpiI GAAGAC 1 cut(s) 815
BplI GAGNNNNNCTC 2 cut(s) 377, 409
BpmI CTGGAG 1 cut(s) 404
BpuEI CTTGAG 2 cut(s) 56, 1215
BsaAI YACGTR 1 cut(s) 629
BsaHI GRCGYC 1 cut(s) 805
BsaJI CCNNGG 3 cut(s) 28, 869, 1203
BsaXI ACNNNNNCTCC 8 cut(s) 108, 138, 215, 245, 910, 940, 1200, 1230
Bsc4I CCNNNNNNNGG 2 cut(s) 476, 1245
Bse118I RCCGGY 1 cut(s) 216
Bse1I ACTGG 3 cut(s) 193, 702, 1133
Bse3DI GCAATG 2 cut(s) 1101, 1155
BseBI CCWGG 2 cut(s) 29, 737
BseDI CCNNGG 3 cut(s) 28, 869, 1203
BseGI GGATG 1 cut(s) 701
BseLI CCNNNNNNNGG 2 cut(s) 476, 1245
BseMI GCAATG 2 cut(s) 1101, 1155
BseNI ACTGG 3 cut(s) 193, 702, 1133
BseX3I CGGCCG 1 cut(s) 218
BseXI GCAGC 8 cut(s) 20, 33, 61, 333, 361, 505, 533, 838
BseYI CCCAGC 1 cut(s) 59
Bsh1236I CGCG 1 cut(s) 1173
Bsh1285I CGRYCG 1 cut(s) 221
BshFI GGCC 4 cut(s) 220, 874, 1188, 1249
BshNI GGYRCC 1 cut(s) 466
BsiEI CGRYCG 1 cut(s) 221
BsiSI CCGG 3 cut(s) 217, 221, 907
BslFI GGGAC 2 cut(s) 1137, 1222
BslI CCNNNNNNNGG 2 cut(s) 476, 1245
BsmAI GTCTC 3 cut(s) 380, 548, 930
BsmFI GGGAC 2 cut(s) 1137, 1222
BsnI GGCC 4 cut(s) 220, 874, 1188, 1249
Bsp143I GATC 2 cut(s) 814, 976
BspACI CCGC 4 cut(s) 371, 1108, 1173, 1265
BspANI GGCC 4 cut(s) 220, 874, 1188, 1249
BspFNI CGCG 1 cut(s) 1173
BspLI GGNNCC 2 cut(s) 423, 468
BspMAI CTGCAG 3 cut(s) 51, 351, 523
BspPI GGATC 1 cut(s) 809
BspQI GCTCTTC 1 cut(s) 70
BspT107I GGYRCC 1 cut(s) 466
BsrDI GCAATG 2 cut(s) 1101, 1155
BsrFI RCCGGY 1 cut(s) 216
BsrI ACTGG 3 cut(s) 193, 702, 1133
BssAI RCCGGY 1 cut(s) 216
BssECI CCNNGG 3 cut(s) 28, 869, 1203
BssMI GATC 2 cut(s) 814, 976
BssNI GRCGYC 1 cut(s) 805
BssT1I CCWWGG 2 cut(s) 869, 1203
Bst2UI CCWGG 2 cut(s) 29, 737
Bst4CI ACNGT 5 cut(s) 498, 535, 542, 560, 1052
Bst6I CTCTTC 3 cut(s) 70, 230, 649
BstACI GRCGYC 1 cut(s) 805
BstAPI GCANNNNNTGC 1 cut(s) 518
BstBAI YACGTR 1 cut(s) 629
BstC8I GCNNGC 3 cut(s) 369, 567, 1171
BstDEI CTNAG 4 cut(s) 303, 921, 1115, 1278
BstF5I GGATG 1 cut(s) 701
BstFNI CGCG 1 cut(s) 1173
BstH2I RGCGCY 1 cut(s) 55
BstHHI GCGC 1 cut(s) 54
BstKTI GATC 2 cut(s) 817, 979
BstMAI GTCTC 3 cut(s) 380, 548, 930
BstMBI GATC 2 cut(s) 814, 976
BstMCI CGRYCG 1 cut(s) 221
BstMWI GCNNNNNNNGC 4 cut(s) 60, 421, 518, 1166
BstNI CCWGG 2 cut(s) 29, 737
BstNSI RCATGY 5 cut(s) 315, 371, 393, 569, 1142
BstSCI CCNGG 2 cut(s) 27, 735
BstSFI CTRYAG 3 cut(s) 47, 347, 519
BstUI CGCG 1 cut(s) 1173
BstV1I GCAGC 8 cut(s) 20, 33, 61, 333, 361, 505, 533, 838
BstV2I GAAGAC 1 cut(s) 815
BstZI CGGCCG 1 cut(s) 218
BsuRI GGCC 4 cut(s) 220, 874, 1188, 1249
BtsCI GGATG 1 cut(s) 701
BtsIMutI CAGTG 2 cut(s) 21, 186
Cac8I GCNNGC 3 cut(s) 369, 567, 1171
CfoI GCGC 1 cut(s) 54
Cfr10I RCCGGY 1 cut(s) 216
Cfr13I GGNCC 2 cut(s) 909, 1087
CseI GACGC 1 cut(s) 671
Csp6I GTAC 1 cut(s) 626
CspCI CAANNNNNGTGG 2 cut(s) 918, 953
CviAII CATG 7 cut(s) 312, 329, 368, 390, 566, 1091, 1139
CviQI GTAC 1 cut(s) 626
DdeI CTNAG 4 cut(s) 303, 921, 1115, 1278
DpnI GATC 2 cut(s) 816, 978
DpnII GATC 2 cut(s) 814, 976
DraI TTTAAA 2 cut(s) 265, 952
DrdI GACNNNNNNGTC 1 cut(s) 1234
DseDI GACNNNNNNGTC 1 cut(s) 1234
EaeI YGGCCR 2 cut(s) 218, 872
EagI CGGCCG 1 cut(s) 218
Eam1104I CTCTTC 3 cut(s) 70, 230, 649
EarI CTCTTC 3 cut(s) 70, 230, 649
EclXI CGGCCG 1 cut(s) 218
Eco130I CCWWGG 2 cut(s) 869, 1203
Eco47I GGWCC 2 cut(s) 909, 1087
Eco52I CGGCCG 1 cut(s) 218
Eco57I CTGAAG 2 cut(s) 201, 996
EcoRII CCWGG 2 cut(s) 27, 735
EcoT14I CCWWGG 2 cut(s) 869, 1203
ErhI CCWWGG 2 cut(s) 869, 1203
FaeI CATG 7 cut(s) 315, 332, 371, 393, 569, 1094, 1142
FalI AAGNNNNNCTT 2 cut(s) 951, 983
FaqI GGGAC 2 cut(s) 1137, 1222
FatI CATG 7 cut(s) 311, 328, 367, 389, 565, 1090, 1138
FbaI TGATCA 1 cut(s) 976
FblI GTMKAC 1 cut(s) 660
FokI GGATG 1 cut(s) 688
FspBI CTAG 1 cut(s) 900
GlaI GCGC 1 cut(s) 53
GsaI CCCAGC 1 cut(s) 63
GsuI CTGGAG 1 cut(s) 404
HaeII RGCGCY 1 cut(s) 55
HaeIII GGCC 4 cut(s) 220, 874, 1188, 1249
HapII CCGG 3 cut(s) 217, 221, 907
HgaI GACGC 1 cut(s) 671
HhaI GCGC 1 cut(s) 54
Hin1I GRCGYC 1 cut(s) 805
Hin1II CATG 7 cut(s) 315, 332, 371, 393, 569, 1094, 1142
Hin6I GCGC 1 cut(s) 52
HinP1I GCGC 1 cut(s) 52
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HinfI GANTC 5 cut(s) 5, 11, 1150, 1228, 1235
HpaII CCGG 3 cut(s) 217, 221, 907
HphI GGTGA 3 cut(s) 611, 1040, 1097
Hpy166II GTNNAC 7 cut(s) 157, 538, 556, 619, 661, 733, 896
Hpy188I TCNGA 5 cut(s) 10, 491, 918, 973, 1015
Hpy188III TCNNGA 4 cut(s) 73, 131, 383, 1232
Hpy8I GTNNAC 7 cut(s) 157, 538, 556, 619, 661, 733, 896
HpyAV CCTTC 3 cut(s) 128, 164, 771
HpyCH4III ACNGT 5 cut(s) 498, 535, 542, 560, 1052
HpyCH4IV ACGT 2 cut(s) 628, 805
HpyF10VI GCNNNNNNNGC 4 cut(s) 60, 421, 518, 1166
HpyF3I CTNAG 4 cut(s) 303, 921, 1115, 1278
HpySE526I ACGT 2 cut(s) 628, 805
Hsp92I GRCGYC 1 cut(s) 805
Hsp92II CATG 7 cut(s) 315, 332, 371, 393, 569, 1094, 1142
HspAI GCGC 1 cut(s) 52
Ksp22I TGATCA 1 cut(s) 976
Kzo9I GATC 2 cut(s) 814, 976
LguI GCTCTTC 1 cut(s) 70
LmnI GCTCC 1 cut(s) 421
Lsp1109I GCAGC 8 cut(s) 20, 33, 61, 333, 361, 505, 533, 838
LweI GCATC 2 cut(s) 100, 710
MaeI CTAG 1 cut(s) 900
MaeII ACGT 2 cut(s) 628, 805
MaeIII GTNAC 5 cut(s) 184, 646, 1131, 1180, 1270
MalI GATC 2 cut(s) 816, 978
MboI GATC 2 cut(s) 814, 976
MboII GAAGA 7 cut(s) 57, 221, 247, 666, 728, 800, 815
MfeI CAATTG 2 cut(s) 426, 876
MlsI TGGCCA 1 cut(s) 874
MluCI AATT 6 cut(s) 291, 426, 603, 860, 876, 1251
MluNI TGGCCA 1 cut(s) 874
MlyI GAGTC 3 cut(s) 1159, 1222, 1244
MmeI TCCRAC 1 cut(s) 484
MnlI CCTC 9 cut(s) 51, 80, 133, 169, 457, 485, 933, 1052, 1056
Mox20I TGGCCA 1 cut(s) 874
MroXI GAANNNNTTC 1 cut(s) 967
MscI TGGCCA 1 cut(s) 874
MseI TTAA 3 cut(s) 198, 264, 951
MslI CAYNNNNRTG 2 cut(s) 108, 993
Msp20I TGGCCA 1 cut(s) 874
MspI CCGG 3 cut(s) 217, 221, 907
MspR9I CCNGG 2 cut(s) 29, 737
MunI CAATTG 2 cut(s) 426, 876
MvaI CCWGG 2 cut(s) 29, 737
MvnI CGCG 1 cut(s) 1173
MwoI GCNNNNNNNGC 4 cut(s) 60, 421, 518, 1166
NdeII GATC 2 cut(s) 814, 976
NlaIII CATG 7 cut(s) 315, 332, 371, 393, 569, 1094, 1142
NlaIV GGNNCC 2 cut(s) 423, 468
NmuCI GTSAC 3 cut(s) 184, 1180, 1270
NspI RCATGY 5 cut(s) 315, 371, 393, 569, 1142
PaeI GCATGC 2 cut(s) 371, 569
PciI ACATGT 1 cut(s) 1138
PciSI GCTCTTC 1 cut(s) 70
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 802
PdmI GAANNNNTTC 1 cut(s) 967
PfeI GAWTC 1 cut(s) 11
PflFI GACNNNGTC 1 cut(s) 922
PflMI CCANNNNNTGG 1 cut(s) 476
PleI GAGTC 3 cut(s) 1158, 1222, 1243
PpsI GAGTC 3 cut(s) 1158, 1222, 1243
Ppu21I YACGTR 1 cut(s) 629
PscI ACATGT 1 cut(s) 1138
Psp6I CCWGG 2 cut(s) 27, 735
PspFI CCCAGC 1 cut(s) 59
PspGI CCWGG 2 cut(s) 27, 735
PspN4I GGNNCC 2 cut(s) 423, 468
PspPI GGNCC 2 cut(s) 909, 1087
PstI CTGCAG 3 cut(s) 51, 351, 523
PsyI GACNNNGTC 1 cut(s) 922
RsaI GTAC 1 cut(s) 627
RsaNI GTAC 1 cut(s) 626
RseI CAYNNNNRTG 2 cut(s) 108, 993
SapI GCTCTTC 1 cut(s) 70
SaqAI TTAA 3 cut(s) 198, 264, 951
Sau3AI GATC 2 cut(s) 814, 976
Sau96I GGNCC 2 cut(s) 909, 1087
SchI GAGTC 3 cut(s) 1159, 1222, 1244
ScrFI CCNGG 2 cut(s) 29, 737
SfaNI GCATC 2 cut(s) 100, 710
SfcI CTRYAG 3 cut(s) 47, 347, 519
SinI GGWCC 2 cut(s) 909, 1087
SmiMI CAYNNNNRTG 2 cut(s) 108, 993
SmlI CTYRAG 2 cut(s) 71, 1230
SmoI CTYRAG 2 cut(s) 71, 1230
SphI GCATGC 2 cut(s) 371, 569
Sse9I AATT 6 cut(s) 291, 426, 603, 860, 876, 1251
SsiI CCGC 4 cut(s) 371, 1108, 1173, 1265
SspMI CTAG 1 cut(s) 900
StyD4I CCNGG 2 cut(s) 27, 735
StyI CCWWGG 2 cut(s) 869, 1203
TaaI ACNGT 5 cut(s) 498, 535, 542, 560, 1052
TaiI ACGT 2 cut(s) 631, 808
TaqI TCGA 2 cut(s) 652, 838
TasI AATT 6 cut(s) 291, 426, 603, 860, 876, 1251
TauI GCSGC 2 cut(s) 1176, 1268
TfiI GAWTC 1 cut(s) 11
Tru1I TTAA 3 cut(s) 198, 264, 951
Tru9I TTAA 3 cut(s) 198, 264, 951
TscAI CASTG 2 cut(s) 21, 193
TseFI GTSAC 3 cut(s) 184, 1180, 1270
TseI GCWGC 8 cut(s) 33, 46, 49, 346, 349, 518, 521, 851
Tsp45I GTSAC 3 cut(s) 184, 1180, 1270
TspDTI ATGAA 4 cut(s) 151, 222, 288, 345
TspRI CASTG 2 cut(s) 21, 193
Tth111I GACNNNGTC 1 cut(s) 922
Van91I CCANNNNNTGG 1 cut(s) 476
VpaK11BI GGWCC 2 cut(s) 909, 1087
XapI RAATTY 1 cut(s) 860
XceI RCATGY 5 cut(s) 315, 371, 393, 569, 1142
XcmI CCANNNNNNNNNTGG 1 cut(s) 360
XmiI GTMKAC 1 cut(s) 660
XmnI GAANNNNTTC 1 cut(s) 967
XspI CTAG 1 cut(s) 900
ZraI GACGTC 1 cut(s) 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.